First data on the genital anatomy of the genus Jeanneretia (: Cepolidae), endemic of the western region of Cuba

Maike Hernández Quinta1 & Thierry Backeljau2,3

1Institute of Ecology and Systematics, Carretera Varona Km 3 1/5, Capdevila, Havana, Cuba Email: [email protected] 2Royal Belgian Institute of Natural Sciences, Vautierstraat 29, B-1000 Brussels, Belgium Email: [email protected] 3Evolutionary Ecology Group, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium

Introduction The genus Jeanneretia Pfeiffer, 1877 (, Helicoidea: Cepolidae) is an endemic taxon in western Cuba J. parraiana ssp2 Figures J. parraiana ssp1 so (Province of Matanzas to the Province of Pinar del Rio, and on the Isle of Pines). It comprizes two subgenera, viz. aco agls ag Jeanneretia s.s. (4 species) without and Guladentia (5 species) with a gular fold (fig. 9c). However, all Jeanneretia hd species were described on conchological features only (1), so that the taxonomy of this genus needs to be consolidated. 6a As such, we provide here the very first anatomical data of several Jeanneretia species, representing the two subgenera. aco so ag 6b 7d Distribution of the genus and localities of sampling. pe agl bcd bcd vd e bc fl 7b bc gland duct 6c pe aco e aco fl - J. bicincta: 1, 2, 3 - J. p. ssp2: 11 copulatory - J. sp: 4 - J. torrei: 11 organ - J. parraiana ssp1: 5 - J. subtussulcata: 7, 8 7a as - J. p. carinata: 6,7 mg Black bar- 2 mm - J. p. depressa: 8 as 7c Orange bar- 1mm - J. p. arangoi: 9, 10 Distribution limit

Measurements of the morphological variables of the reproductive organs in Jeanneretia species. Length J. bicicta J. p. carinata J. p. depressa J. p. arangoi J. p. ssp2 J. p. ssp1 J. sp J. subtussulcata (mm) (7) (8) (13) (3) (1) (1) (13) (3) Materials y methods X± SD X± SD X ± SD Min - Max X ± SD Min - Max Jeanneretia species were fixed in 70% ethanol. Adult specimens were dissected and the genital anatomy was studied fl 78 ± 18 64 ± 11 77 ± 27 85 - 98 260 95 90 ± 8 23 – 25 pe 5 ± 1 7 ± 1 7 ± 1 6 - 7 14 7 7 ± 1 6 – 9 using a Carl Zeiss Stereomicroscope. Subsequently, the reproductive organs were drawn from photographs taken ep 4 ± 1 4 ± 1 4 ± 1 4 13 4 4 ± 1 10 – 15 with a Canon camera and a Dino-Eye Digital Microscope Eyepiece Camera. aco 12 ± 5 8 ± 3 8 ± 1 11 - 15 22 20 11 ± 2 11 agl 17 ± 4 13 ± 3 14 ± 3 18 - 25 30 18 25 ± 4 6 - 9 bcd 56 ± 23 44 ± 13 37 ± 15 29 - 59 80 80 44 ± 12 23 - 33 Results so 17 ± 3 22 ± 5 25 ± 5 20 - 21 38 - 20 ± 4 35 - 40 The subgenus Jeanneretia has a long, slender penis (pe) and a cylindrical epiphallus (e) with a weak retractor muscle

(rm). The distal tip of the epiphallus splits into a long flagellum (fl) (fig: 1-7) and the vas deferens (vd). The vagina The genitalia of the subgenus Guladentia are similar to those of Jeanneretia. The flagellum and the bursa copulatrix (va) is short and gives rise to the duct of the bursa copulatrix (bcd) and the oviduct (ov). The bursa copulatrix is duct can be short or long. The epiphallus is longer than the penis (fig. 8b, 9b). This subgenus can have an only elongated and the duct is longer than the spermoviduct (so), but (most often) shorter than the flagellum (fig: 1-7). The alveolar gland (8c) or a gland formed by two cylindrical lobes (9b). carrefour presents a fertilization pouch and single, long spermathecal tube (sp) (fig 5b). The auxiliary copulatory Figures organ (aco), unlike the rests of the cepolids, consists of (1) an elongated gland (mg) inserted apically on a muscular J. subtusulcata J. torrei papilla, (2) a dart-like structure (covered by a sheath, fig. 7d), and (3) a two accessory tubular glands (agls) inserted in ag 8a bc the base of the atrial sac (ac). The dissected individuals did not show a dart, while their duct of the mucus gland was aco 8d 9c hd connected directly with the muscular papilla (fig. 7c), which continues with a similar structure to a dart (fig. 2d). mg hd papilla bcd bc ag Gular fold fl Figures J. bicincta 1a J. sp as 2a fl bc so so vd bcd pg ag agl bcd rm 2c e ov hd mg aco 9a e aco hd pe aco agl va agls sp pe bc so bcd papilla so ag 1c Black bar- 2 mm aco pg 8b pg fl agl 8c Orange bar- 1 mm 9b aco agl as mg vd Jeanneretia ss. differs much from other cepolid subgenera, the main difference being the absence of a dart apparatus agls ov aco va rm as (da) typical of the family (fig. 10-14). Other cepolids lack a dart sac (ds) and/or a dart (2). The accessory glands are e e pe the longest in the family, and they are not joined as the remainders genus (except Eurycampta) where they can be fl pe 1b 2b joined in the base (fig. 11), unit for a common duct (10), bilobed (12) or an unique gland (fig. 9b, 14). The presence 2d of a flagellum and a duct of the bursa long copulatrix distinguish the subgenus. J. parraiana depressa 3a 4a J. p. carinata Figures sp Cysticopsis naevula muscarum ag ag hd 4d ag 10 12 hd 11 3d sp ag bc 3c hd Blackmg bae sp so bc bc so pg bcd so papilla da pg so bc ds so agls mg bc aco agls as ov fl aco da agl agl papilla mg pe aco agl ov bcd fl as ds prm agl pe e 4c pe agl fl fl pe aco fl bc e 3b 4b Eurycampta pinarensis ag hd 13 Setipellis stigmatica J. p. arangoi bcd 5a mg ag 5c fl so 14 pg as pg bc bcd so agl so aco bc da fl pe va mg aco as Black bar- 2 mm agls agl sheath 5d fl References Black bar- 2 mm (1) Clench J.W. and Aguayo, C. G. (1951): The Cuban genus Jeanneretia. Revista de la Sociedad Malacológica “Carlos Orange bar- 1 mm papilla e pe de la Torre”. 7 (3): 81-92. 5b (2) Méndez, A. 2009. Variabilidad del dardo en especies de moluscos de la familia Cepolidae: implicaciones para la selección sexual. Thesis Master. B.Sc. Dissertation. 65 pp.