bioRxiv preprint doi: https://doi.org/10.1101/2021.02.22.432361; this version posted February 24, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. TP-MAP - an Integrated Software Package for the Analysis of 1D and 2D Thermal Profiling Data Felix Feyertag1,2 and Kilian V.M. Huber1,2* 1 Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom 2 Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom * Corresponding author:
[email protected] Abstract Thermal profiling (TP) has emerged as a promising experimental methodology for elucidating the molecular targets of drugs and metabolites on a proteome-wide scale. Here, we present the Thermal Profiling Meltome Analysis Program (TP-MAP) software package for the analysis and ranking of 1D and 2D thermal profiling datasets. TP-MAP provides a user-friendly interface to quickly identify hit candidates and further explore targets of interest via intersection and crosslinking to public databases. 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.22.432361; this version posted February 24, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Introduction Assessing molecular perturbations in living cells caused by drug treatment, environmental changes, genetic mutations or alterations in metabolic flux in an unbiased and proteome- wide manner constitutes a key challenge in chemical biology and systems pharmacology.