Monoclonal Antibody to CD305 / LAIR1

SM6003 OriGene Technologies Inc. OriGene EU

Acris Antibodies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected]

Monoclonal Antibody to CD305 / LAIR1 - Purified Alternate names: LAIR-1, Leukocyte-associated immunoglobulin-like receptor 1, hLAIR1 Catalog No.: SM6003 Quantity: 0.1 ml Concentration: 1.0 mg/ml Background: LAIR-1 is a member of the leukocyte-associated Ig like receptor family. The LAIR-1 is a transmembrane glycoprotein (~32kDa) expressed on the majority of human peripheral blood mononuclear leukocytes including T cells , B cells, NK cells, macrophages and dendritic cells (1,2). LAIR-1 recruits SHP-1 and SHP-2 tyrosine phosphatases upon activation, and cross-linking of the LAIR-1 molecule on NK cells results in strong inhibition of NK cell-mediated cytotoxicity. Uniprot ID: Q6GTX8 NCBI: NP_002278.1 GeneID: 3903 Host / Isotype: Mouse / IgG1 Recommended SM10P (for use in human samples), AM03095PU-N Isotype Controls: Clone: LC12 Immunogen: Recombinant Human LAIR-1 (22-125 aa) purified from E. coli Format: State: Liquid purified Ig fraction Purification: Protein-G affinity chromatography Buffer System: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol Applications: ELISA. Western blot (Recombinant protein). Flow Cytometry. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: Reacts with extracellular domain of the LAIR-1. Does not react with KIR2DL1, KIR2DL3 and KIR2DS4 in ELISA or Western blot. Species: Human. Other species not tested. Add. Information: This product is routinely tested on peripheral blood mononuclear cells (PBMCs) or natural killer(NK) cells. The histogram is an example of the expected reactivity.

For research and in vitro use only. Not for diagnostic or therapeutic work. MS/20150812 Material Safety Datasheets are available at www.acris-antibodies.com or on request. Acris Antibodies is now part of the OriGene family. Learn more at www.origene.com 1 / 2 SM6003: Monoclonal Antibody to CD305 / LAIR1 - Purified Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Meyaard L, Adema GJ, Chang C, Woollatt E, Sutherland GR, Lanier LL, et al. LAIR-1, a novel inhibitory receptor expressed on human mononuclear leukocytes. Immunity. 1997 Aug;7(2):283-90. PubMed PMID: 9285412. 2. Meyaard L, Hurenkamp J, Clevers H, Lanier LL, Phillips JH. Leukocyte-associated Ig-like receptor-1 functions as an inhibitory receptor on cytotoxic T cells. J Immunol. 1999 May 15;162(10):5800-4. PubMed PMID: 10229813. Pictures: Immunohistochemistry: SM6003 CD305/LAIR1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase- streptavidin and chromogen.

Profile of LAIR-1 positive PBMC cells analyzed by Flow Cytometry.