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United States Department of the Interior FISH AND WILDLIFE SERVICE Ecological Services Carlsbad Fish and Wildlife Office 2177 Salk Avenue, Suite 250 Carlsbad, California 92008 Study Guide for Vernal Pool Branchiopod Test The following is a list of the vernal pool branchiopod species that are listed or that are most likely to be found in or near the same geographical range as a listed species. Any or all species from this list may be included in the identification test. 1. Artemia sp. (ID to genus OK) 2. Branchinecta coloradensis 3. Branchinecta conservatio 4. Branchinecta dissmilis 5. Branchinecta gigas 6. Branchinecta lindahli 7. Branchinecta lynchi 8. Branchinecta mackini 9. Branchinecta mesovallensis 10. Branchinecta sandiegonensis 11. Eubranchipus serratus (ID to genus OK) 12. Eubranchipus oregonus (ID to genus OK) 13. Lepidurus packardi 14. Linderiella occidentalis (ID to genus OK) 15. Streptocephalus sealii 16. Streptocephalus woottoni 17. Triops longicaudatus (ID to genus OK) 18. Branchinecta longiantenna The test will be set up with approximately 17 to 20 single-species vials, with one to six specimens in each, and with an additional two or three vials containing two species together. We require that all listed species on the permit being applied for or renewed be accurately identified, along with all species likely to be found near the listed species geographic range. We give the test by appointment. We encourage those taking the test to bring their own stereoscopes and any reference materials they require to make appropriate identifications. "Soft" forceps, watchglasses, and probes will be provided. When making the appointment, allow enough time to complete the test without rushing (the average time is 3 to 4 hours, but can range from 2 to 8 hours). Please call the Recovery Permit Coordinator at (760) 431-9440 to schedule a test appointment at the Carlsbad Fish and Wildlife Office. Appointments can also be made at the Sacramento Fish and Wildlife Office at (916) 414-6600. .

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Fig. Ap. 2.1. Denton Tending His Fairy Shrimp Collection
Fig. Ap. 2.1. Denton tending his fairy shrimp collection. 176 Appendix 1 Hatching and Rearing Back in the bowels of this book we noted that However, salts may leach from soils to ultimately if one takes dry soil samples from a pool basin, make the water salty, a situation which commonly preferably at its deepest point, one can then "just turns off hatching. Tap water is usually unsatis- add water and stir". In a day or two nauplii ap- factory, either because it has high TDS, or because pear if their cysts are present. O.K., so they won't it contains chlorine or chloramine, disinfectants always appear, but you get the idea. which may inhibit hatching or kill emerging If your desire is to hatch and rear fairy nauplii. shrimps the hi-tech way, you should get some As you have read time and again in Chapter 5, guidance from Brendonck et al. (1990) and temperature is an important environmental cue for Maeda-Martinez et al. (1995c). If you merely coaxing larvae from their dormant state. You can want to see what an anostracan is like, buy some guess what temperatures might need to be ap- Artemia cysts at the local aquarium shop and fol- proximated given the sample's origin. Try incu- low directions on the container. Should you wish bation at about 3-5°C if it came from the moun- to find out what's in your favorite pool, or gather tains or high desert. If from California grass- together sufficient animals for a study of behavior lands, 10° is a good level at which to start.
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