Epitect® Methyl II Complete PCR Array (94) Human Leukemia & Lymphoma

EpiTect® Methyl II Complete PCR Array (94) Human Leukemia & Lymphoma

Cat. no. 335222 EAHS-8070ZA

For DNA methylation analysis using MethylScreen™ technology

The Human Leukemia & Lymphoma EpiTect Methyl II Complete PCR Arrays profile the promoter methylation status of a panel of 94 tumor suppressor gene promoters whose hypermethylation has been reported in the literature to occur frequently in a variety of leukemia and lymphomas. Profiling cellular or fresh tissue genomic DNA samples with these arrays may help correlate CpG island methylation status with biological phenotypes. The results may also provide insights into the molecular mechanisms and biological pathways behind oncogenesis and cancer pathology. With a simple restriction enzyme digestion and real-time PCR, research studies can analyze the promoter methylation status of 94 different leukemia and lymphoma genes with this DNA methylation PCR array. The EpiTect Methyl II PCR Arrays use MethylScreen™ technology provided under license from Orion Genomics, LLC. For further details, consult the EpiTect Methyl II PCR Array Handbook.

Shipping and storage

EpiTect Methyl II Complete PCR Arrays are shipped at room temperature (15–25°C), on dry ice, or on blue ice depending on the destination and accompanying products. They should be stored at –20°C. Ensure that you have the correct EpiTect Methyl II Complete PCR Array format for your instrument before starting the experiment.

Note: Open the package and store the products appropriately immediately on receipt.

Sample & Assay Technologies Contents

Product Contents Cat. no.

EpiTect Methyl II Complete 2 x set of 4 of 96-well plates, 12 Optical Thin-Wall Varies PCR Array (94) Format A 8-Cap Strips; for use with the following real-time cyclers: Applied Biosystems® models 5700, 7000, 7300, 7500, 7700, 7900HT, ViiA™ 7 (96-well block); Bio-Rad® models iCycler®, iQ™5, MyiQ™, MyiQ2; Bio-Rad/MJ Research Chromo4™; Eppendorf® MasterCycler® ep realplex models 2, 2s, 4, 4s; Stratagene® models Mx3005P®, Mx3000P ®

EpiTect Methyl II Complete 2 x set of 4 of 96-well plates; Optical Adhesive Film; Varies PCR Array (94) Format C for use with the following real-time cyclers: Applied Biosystems models 7500 (Fast block), 7900HT (Fast block), StepOnePlus™, ViiA 7 (Fast block)

EpiTect Methyl II Complete 2 x set of 4 of x 96-well plates, 12 Optical Thin-Wall Varies PCR Array (94) Format D 8-Cap Strips; for use with the following real-time cyclers: Bio-Rad CFX96™; Bio-Rad/MJ Research models DNA Engine Opticon®, DNA Engine Opticon 2; Stratagene Mx4000®

EpiTect Methyl II Complete 2, 12, or 24 x 384-well plates; 2, 12, or 24 sets of Varies PCR Array (94) Format E 384EZLoad Covers; Optical Adhesive Film; for use with the following real-time cyclers: Applied Biosystems models 7900HT (384-well block), ViiA 7 (384-well block); Bio-Rad CFX384™

EpiTect Methyl II Complete 2 x set of 4 of 96-well plates; Optical Adhesive Film; Varies PCR Array (94) Format F for use with the following real-time cyclers: Roche® LightCycler® 480 (96-well block)

EpiTect Methyl II Complete 2, 12, or 24 x 384-well plates; 2, 12, or 24 sets of Varies PCR Array (94) Format G 384EZLoad Covers; Optical Adhesive Film; for use with the following real-time cyclers: Roche LightCycler 480 (384-well block)

Array layout EAHS-071Z

1 2 3 4 5 6 7 8 9 10 11 12

A AFF1 CD9 CEBPD CTNNA1 DRD2 EXT1 FANCC FANCL HCK HOXA7 HOXB5 JUNB

B LMNA MAFB MEN1 NFATC1 NPM1 PER2 SLC5A8 SPOCK2 TLE1 TP53 EP_SEC EP_DEC

C AFF1 CD9 CEBPD CTNNA1 DRD2 EXT1 FANCC FANCL HCK HOXA7 HOXB5 JUNB

D LMNA MAFB MEN1 NFATC1 NPM1 PER2 SLC5A8 SPOCK2 TLE1 TP53 EP_SEC EP_DEC

E AFF1 CD9 CEBPD CTNNA1 DRD2 EXT1 FANCC FANCL HCK HOXA7 HOXB5 JUNB

F LMNA MAFB MEN1 NFATC1 NPM1 PER2 SLC5A8 SPOCK2 TLE1 TP53 EP_SEC EP_DEC

G AFF1 CD9 CEBPD CTNNA1 DRD2 EXT1 FANCC FANCL HCK HOXA7 HOXB5 JUNB

H LMNA MAFB MEN1 NFATC1 NPM1 PER2 SLC5A8 SPOCK2 TLE1 TP53 EP_SEC EP_DEC

EAHS-072Z

1 2 3 4 5 6 7 8 9 10 11 12

A ABCG2 AHR ALOX12 APAF1 APC ARF4 ARHGAP29 ASNS ATM BNIP3 CADM1 CCND1

B CDH1 CDKN1B CDKN1C CDKN2C CHFR CTNNB1 DAPK1 DBC1 DPP4 DTL EGR3 EPHA7

C ABCG2 AHR ALOX12 APAF1 APC ARF4 ARHGAP29 ASNS ATM BNIP3 CADM1 CCND1

D CDH1 CDKN1B CDKN1C CDKN2C CHFR CTNNB1 DAPK1 DBC1 DPP4 DTL EGR3 EPHA7

E ABCG2 AHR ALOX12 APAF1 APC ARF4 ARHGAP29 ASNS ATM BNIP3 CADM1 CCND1

F CDH1 CDKN1B CDKN1C CDKN2C CHFR CTNNB1 DAPK1 DBC1 DPP4 DTL EGR3 EPHA7

G ABCG2 AHR ALOX12 APAF1 APC ARF4 ARHGAP29 ASNS ATM BNIP3 CADM1 CCND1

H CDH1 CDKN1B CDKN1C CDKN2C CHFR CTNNB1 DAPK1 DBC1 DPP4 DTL EGR3 EPHA7

EAHS-073Z

1 2 3 4 5 6 7 8 9 10 11 12

A FBXW7 FHIT GADD45A GFPT2 GGH GIPC2 GPR37 GSTM1 GSTP1 HLA-G HOXB6 HOXB7

B HS3ST2 ID4 IRF4 KLF11 KLF4 LATS1 LATS2 LTF MEG3 MEIS1 MLH1 MME

C FBXW7 FHIT GADD45A GFPT2 GGH GIPC2 GPR37 GSTM1 GSTP1 HLA-G HOXB6 HOXB7

D HS3ST2 ID4 IRF4 KLF11 KLF4 LATS1 LATS2 LTF MEG3 MEIS1 MLH1 MME

E FBXW7 FHIT GADD45A GFPT2 GGH GIPC2 GPR37 GSTM1 GSTP1 HLA-G HOXB6 HOXB7

F HS3ST2 ID4 IRF4 KLF11 KLF4 LATS1 LATS2 LTF MEG3 MEIS1 MLH1 MME

G FBXW7 FHIT GADD45A GFPT2 GGH GIPC2 GPR37 GSTM1 GSTP1 HLA-G HOXB6 HOXB7

H HS3ST2 ID4 IRF4 KLF11 KLF4 LATS1 LATS2 LTF MEG3 MEIS1 MLH1 MME

EAHS-074Z

1 2 3 4 5 6 7 8 9 10 11 12

A MMP2 MSX2 MT2A OGG1 PARK2 PEG10 PLK2 PMS1 PRAME PROX1 PTEN PTPRO

B PYCARD RARB RB1 SALL1 SDC4 SFRP1 SFRP4 SLC26A4 SLC2A14 THBS1 TNFRSF8 ZAP70

C MMP2 MSX2 MT2A OGG1 PARK2 PEG10 PLK2 PMS1 PRAME PROX1 PTEN PTPRO

D PYCARD RARB RB1 SALL1 SDC4 SFRP1 SFRP4 SLC26A4 SLC2A14 THBS1 TNFRSF8 ZAP70

E MMP2 MSX2 MT2A OGG1 PARK2 PEG10 PLK2 PMS1 PRAME PROX1 PTEN PTPRO

F PYCARD RARB RB1 SALL1 SDC4 SFRP1 SFRP4 SLC26A4 SLC2A14 THBS1 TNFRSF8 ZAP70

G MMP2 MSX2 MT2A OGG1 PARK2 PEG10 PLK2 PMS1 PRAME PROX1 PTEN PTPRO

H PYCARD RARB RB1 SALL1 SDC4 SFRP1 SFRP4 SLC26A4 SLC2A14 THBS1 TNFRSF8 ZAP70 Gene table EAHS-071Z

GenBank OR Gene Position Unigene miRNA Symbol OR Description Gene Name Accession miRNA ID AF4, MGC134969, MLLT2, A01, C01, E01, G01 Hs.480190 NM_005935 AFF1 AF4/FMR2 family, member 1 PBM1 BTCC-1, DRAP-27, FLJ99568, A02, C02, E02, G02 Hs.114286 NM_001769 CD9 CD9 molecule MIC3, MRP-1, TSPAN-29, TSPAN29 CCAAT/enhancer binding protein C, EBP-delta, CELF, CRP3, A03, C03, E03, G03 Hs.440829 NM_005195 CEBPD (C/EBP), delta NF-IL6-beta Catenin (cadherin-associated protein), CAP102, FLJ36832, A04, C04, E04, G04 Hs.534797 NM_001903 CTNNA1 alpha 1, 102kDa FLJ52416 A05, C05, E05, G05 Hs.73893 NM_000795 DRD2 Dopamine receptor D2 D2DR, D2R A06, C06, E06, G06 Hs.492618 NM_000127 EXT1 Exostosin 1 EXT, LGCR, LGS, TRPS2, TTV Fanconi anemia, complementation A07, C07, E07, G07 Hs.494529 NM_000136 FANCC FA3, FAC, FACC, FLJ14675 group C Fanconi anemia, complementation FAAP43, FLJ10335, PHF9, A08, C08, E08, G08 Hs.708194 NM_018062 FANCL group L POG A09, C09, E09, G09 Hs.655210 NM_002110 HCK Hemopoietic cell kinase JTK9 ANTP, HOX1, HOX1.1, A10, C10, E10, G10 Hs.446318 NM_006896 HOXA7 Homeobox A7 HOX1A HHO.C10, HOX2, HOX2A, A11, C11, E11, G11 Hs.654456 NM_002147 HOXB5 Homeobox B5 HU-1, Hox2.1 A12, C12, E12, G12 Hs.25292 NM_002229 JUNB Jun B proto-oncogene AP-1 CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, B01, D01, F01, H01 Hs.594444 NM_005572 LMNA Lamin A/C HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, PRO1 V-maf musculoaponeurotic B02, D02, F02, H02 Hs.169487 NM_005461 MAFB fibrosarcoma oncogene homolog B KRML, MGC43127 (avian) B03, D03, F03, H03 Hs.423348 NM_000244 MEN1 Multiple endocrine neoplasia I MEAI, MENIN, SCG2 Nuclear factor of activated T-cells, MGC138448, NF-ATC, B04, D04, F04, H04 Hs.534074 NM_172390 NFATC1 cytoplasmic, calcineurin-dependent 1 NFAT2, NFATc Nucleophosmin (nucleolar B05, D05, F05, H05 Hs.557550 NM_199185 NPM1 B23, MGC104254, NPM phosphoprotein B23, numatrin) B06, D06, F06, H06 Hs.58756 NM_022817 PER2 Period homolog 2 (Drosophila) FASPS, KIAA0347 Solute carrier family 5 (iodide AIT, MGC125354, SMCT, B07, D07, F07, H07 Hs.444536 NM_145913 SLC5A8 transporter), member 8 SMCT1 Sparc/osteonectin, cwcv and kazal-like B08, D08, F08, H08 Hs.523009 NM_014767 SPOCK2 FLJ97039, testican-2 domains proteoglycan (testican) 2 Transducin-like enhancer of split 1 B09, D09, F09, H09 Hs.197320 NM_005077 TLE1 ESG, ESG1, GRG1 (E(sp1) homolog, Drosophila) B10, D10, F10, H10 Hs.654481 NM_000546 TP53 Tumor protein p53 FLJ92943, LFS1, P53, TRP53 B11, D11, F11, H11 N/A SA_00148 EP_SEC SEC-sensitive enzyme control assay SEC B12, D12, F12, H12 N/A SA_00149 EP_DEC DEC-dependent enzyme control assay DEC

EAHS-072Z

GenBank OR Gene Position Unigene miRNA Symbol OR Description Gene Name Accession miRNA ID ABC15, ABCP, BCRP, BCRP1, ATP-binding cassette, sub-family G BMDP, CD338, CDw338, A01, C01, E01, G01 Hs.480218 NM_004827 ABCG2 (WHITE), member 2 EST157481, MGC102821, MRX, MXR, MXR1 A02, C02, E02, G02 Hs.171189 NM_001621 AHR Aryl hydrocarbon receptor bHLHe76 A03, C03, E03, G03 Hs.654431 NM_000697 ALOX12 Arachidonate 12-lipoxygenase 12-LOX, 12S-LOX, LOG12 APAF-1, CED4, A04, C04, E04, G04 Hs.728891 NM_001160 APAF1 Apoptotic peptidase activating factor 1 DKFZp781B1145 A05, C05, E05, G05 Hs.158932 NM_000038 APC Adenomatous polyposis coli BTPS2, DP2, DP2.5, DP3, GS A06, C06, E06, G06 Hs.695943 NM_001660 ARF4 ADP-ribosylation factor 4 ARF2 A07, C07, E07, G07 Hs.483238 NM_004815 ARHGAP29 Rho GTPase activating protein 29 PARG1, RP11-255E17.1 Asparagine synthetase A08, C08, E08, G08 Hs.489207 NM_183356 ASNS TS11 (glutamine-hydrolyzing) AT1, ATA, ATC, ATD, ATDC, GenBank OR Gene Position Unigene miRNA Symbol OR Description Gene Name Accession miRNA ID ATE, DKFZp781A0353, A09, C09, E09, G09 Hs.367437 NM_000051 ATM Ataxia telangiectasia mutated MGC74674, TEL1, TELO1 BCL2/adenovirus E1B 19kDa A10, C10, E10, G10 Hs.144873 NM_004052 BNIP3 NIP3 interacting protein 3 BL2, DKFZp686F1789, IGSF4, IGSF4A, MGC149785, MGC51880, A11, C11, E11, G11 Hs.370510 NM_014333 CADM1 Cell adhesion molecule 1 NECL2, Necl-2, RA175, ST17, SYNCAM, TSLC1, sTSLC-1, sgIGSF, synCAM1 BCL1, D11S287E, PRAD1, A12, C12, E12, G12 Hs.523852 NM_053056 CCND1 Cyclin D1 U21B31 Cadherin 1, type 1, E-cadherin Arc-1, CD324, CDHE, ECAD, B01, D01, F01, H01 Hs.461086 NM_004360 CDH1 (epithelial) LCAM, UVO Cyclin-dependent kinase inhibitor 1B CDKN4, KIP1, MEN1B, B02, D02, F02, H02 Hs.238990 NM_004064 CDKN1B (p27, Kip1) MEN4, P27KIP1 Cyclin-dependent kinase inhibitor 1C B03, D03, F03, H03 Hs.106070 NM_000076 CDKN1C BWCR, BWS, KIP2, WBS, p57 (p57, Kip2) Cyclin-dependent kinase inhibitor 2C B04, D04, F04, H04 Hs.728783 NM_078626 CDKN2C INK4C, p18, p18-INK4C (p18, inhibits CDK4) Checkpoint with forkhead and ring FLJ10796, FLJ33629, B05, D05, F05, H05 Hs.656770 NM_018223 CHFR finger domains RNF116, RNF196 Catenin (cadherin-associated protein), CTNNB, DKFZp686D02253, B06, D06, F06, H06 Hs.476018 NM_001904 CTNNB1 beta 1, 88kDa FLJ25606, FLJ37923 B07, D07, F07, H07 Hs.380277 NM_004938 DAPK1 Death-associated protein kinase 1 DAPK, DKFZp781I035 B08, D08, F08, H08 Hs.532316 NM_014618 DBC1 Deleted in bladder cancer 1 DBCCR1, FAM5A ADABP, ADCP2, CD26, B09, D09, F09, H09 Hs.368912 NM_001935 DPP4 Dipeptidyl-peptidase 4 DPPIV, TP103 B10, D10, F10, H10 Hs.656473 NM_016448 DTL Denticleless homolog (Drosophila) CDT2, DCAF2, L2DTL, RAMP B11, D11, F11, H11 Hs.534313 NM_004430 EGR3 Early growth response 3 EGR-3, MGC138484, PILOT B12, D12, F12, H12 Hs.73962 NM_004440 EPHA7 EPH receptor A7 EHK3, HEK11

EAHS-073Z

GenBank OR Gene Position Unigene miRNA Symbol OR Description Gene Name Accession miRNA ID AGO, CDC4, DKFZp686F23254, FBW6, F-box and WD repeat domain A01, C01, E01, G01 Hs.679796 NM_033632 FBXW7 FBW7, FBX30, FBXO30, containing 7 FBXW6, FLJ16457, SEL-10, SEL10 A02, C02, E02, G02 Hs.715588 NM_002012 FHIT Fragile histidine triad gene AP3Aase, FRA3B Growth arrest and A03, C03, E03, G03 Hs.80409 NM_001924 GADD45A DDIT1, GADD45 DNA-damage-inducible, alpha Glutamine-fructose-6-phosphate A04, C04, E04, G04 Hs.696497 NM_005110 GFPT2 FLJ10380, GFAT2 transaminase 2 Gamma-glutamyl hydrolase A05, C05, E05, G05 Hs.78619 NM_003878 GGH (conjugase, folylpolygammaglutamyl GH hydrolase) GIPC PDZ domain containing family, FLJ20075, SEMCAP-2, A06, C06, E06, G06 Hs.659356 NM_017655 GIPC2 member 2 SEMCAP2 G protein-coupled receptor 37 A07, C07, E07, G07 Hs.406094 NM_005302 GPR37 EDNRBL, PAELR, hET(B)R-LP (endothelin receptor type B-like) GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, A08, C08, E08, G08 Hs.301961 NM_000561 GSTM1 Glutathione S-transferase mu 1 GTH4, GTM1, H-B, MGC26563, MU, MU-1 DFN7, FAEES3, GST3, GSTP, A09, C09, E09, G09 Hs.523836 NM_000852 GSTP1 Glutathione S-transferase pi 1 PI Major histocompatibility complex, class A10, C10, E10, G10 Hs.512152 NM_002127 HLA-G MHC-G I, G HOX2, HOX2B, HU-2, Hox-2. A11, C11, E11, G11 Hs.98428 NM_018952 HOXB6 Homeobox B6 2 HHO.C1, HOX2, HOX2C, A12, C12, E12, G12 Hs.436181 NM_004502 HOXB7 Homeobox B7 Hox-2.3 Heparan sulfate (glucosamine) GenBank OR Gene Position Unigene miRNA Symbol OR Description Gene Name Accession miRNA ID B01, D01, F01, H01 Hs.115830 NM_006043 HS3ST2 3-O-sulfotransferase 2 30ST2, 3OST2 Inhibitor of DNA binding 4, dominant B02, D02, F02, H02 Hs.519601 NM_001546 ID4 IDB4, bHLHb27 negative helix-loop-helix protein B03, D03, F03, H03 Hs.401013 NM_002460 IRF4 Interferon regulatory factor 4 LSIRF, MUM1 FKLF, FKLF1, MODY7, TIEG2, B04, D04, F04, H04 Hs.12229 NM_003597 KLF11 Kruppel-like factor 11 Tieg3 B05, D05, F05, H05 Hs.376206 NM_004235 KLF4 Kruppel-like factor 4 (gut) EZF, GKLF LATS, large tumor suppressor, B06, D06, F06, H06 Hs.716697 NM_004690 LATS1 WARTS, wts homolog 1 (Drosophila) LATS, large tumor suppressor, B07, D07, F07, H07 Hs.78960 NM_014572 LATS2 FLJ13161, KPM homolog 2 (Drosophila) B08, D08, F08, H08 Hs.529517 NM_002343 LTF Lactotransferrin GIG12, HLF2, LF FLJ31163, FLJ42589, Maternally expressed 3 (non-protein FLJ44703, FP504, GTL2, B09, D09, F09, H09 Hs.728801 NR_002766 MEG3 coding) NCRNA00023, PRO0518, PRO2160, prebp1 B10, D10, F10, H10 Hs.526754 NM_002398 MEIS1 Meis homeobox 1 MGC43380 MutL homolog 1, colon cancer, COCA2, FCC2, HNPCC, B11, D11, F11, H11 Hs.195364 NM_000249 MLH1 nonpolyposis type 2 (E. coli) HNPCC2, MGC5172, hMLH1 CALLA, CD10, DKFZp686O16152, B12, D12, F12, H12 Hs.307734 NM_000902 MME Membrane metallo-endopeptidase MGC126681, MGC126707, NEP, SFE

EAHS-074Z

GenBank OR Gene Position Unigene miRNA Symbol OR Description Gene Name Accession miRNA ID Matrix metallopeptidase 2 (gelatinase CLG4, CLG4A, MMP-II, A01, C01, E01, G01 Hs.513617 NM_004530 MMP2 A, 72kDa gelatinase, 72kDa type IV MONA, TBE-1 collagenase) CRS2, FPP, HOX8, MSH, PFM, A02, C02, E02, G02 Hs.89404 NM_002449 MSX2 Msh homeobox 2 PFM1 A03, C03, E03, G03 Hs.647371 NM_005953 MT2A Metallothionein 2A MT2 HMMH, HOGG1, MUTM, A04, C04, E04, G04 Hs.380271 NM_002542 OGG1 8-oxoguanine DNA glycosylase OGH1 Parkinson protein 2, E3 ubiquitin A05, C05, E05, G05 Hs.132954 NM_004562 PARK2 AR-JP, LPRS2, PDJ, PRKN protein ligase (parkin) EDR, HB-1, KIAA1051, A06, C06, E06, G06 Hs.147492 NM_001040152 PEG10 Paternally expressed 10 MEF3L, Mar2, Mart2, RGAG3 A07, C07, E07, G07 Hs.398157 NM_006622 PLK2 Polo-like kinase 2 SNK PMS1 postmeiotic segregation DKFZp781M0253, FLJ98259, A08, C08, E08, G08 Hs.111749 NM_000534 PMS1 increased 1 (S. cerevisiae) HNPCC3, PMSL1, hPMS1 Preferentially expressed antigen in A09, C09, E09, G09 Hs.30743 NM_006115 PRAME CT130, MAPE, OIP4 melanoma A10, C10, E10, G10 Hs.585369 NM_002763 PROX1 Prospero homeobox 1 10q23del, BZS, DEC, GLM2, A11, C11, E11, G11 Hs.500466 NM_000314 PTEN Phosphatase and tensin homolog MGC11227, MHAM, MMAC1, PTEN1, TEP1 GLEPP1, NPHS6, PTP-OC, Protein tyrosine phosphatase, receptor A12, C12, E12, G12 Hs.160871 NM_002848 PTPRO PTP-U2, PTPROT, PTPU2, type, O R-PTP-O ASC, CARD5, MGC10332, B01, D01, F01, H01 Hs.499094 NM_013258 PYCARD PYD and CARD domain containing TMS, TMS-1, TMS1 B02, D02, F02, H02 Hs.654490 NM_000965 RARB Retinoic acid receptor, beta HAP, NR1B2, RRB2 OSRC, RB, p105-Rb, pRb, B03, D03, F03, H03 Hs.408528 NM_000321 RB1 Retinoblastoma 1 pp110 B04, D04, F04, H04 Hs.135787 NM_002968 SALL1 Sal-like 1 (Drosophila) HSAL1, Sal-1, TBS, ZNF794 B05, D05, F05, H05 Hs.632267 NM_002999 SDC4 Syndecan 4 MGC22217, SYND4 FRP, FRP-1, FRP1, FrzA, B06, D06, F06, H06 Hs.713546 NM_003012 SFRP1 Secreted frizzled-related protein 1 SARP2 B07, D07, F07, H07 Hs.658169 NM_003014 SFRP4 Secreted frizzled-related protein 4 FRP-4, FRPHE, MGC26498 B08, D08, F08, H08 Hs.571246 NM_000441 SLC26A4 Solute carrier family 26, member 4 DFNB4, EVA, PDS, TDH2B Solute carrier family 2 (facilitated DKFZp564K1672, GLUT14, B09, D09, F09, H09 Hs.655169 NM_153449 SLC2A14 glucose transporter), member 14 SLC2A3P3 THBS, THBS-1, TSP, TSP-1, GenBank OR Gene Position Unigene miRNA Symbol OR Description Gene Name Accession miRNA ID B10, D10, F10, H10 Hs.164226 NM_003246 THBS1 Thrombospondin 1 TSP1 Tumor necrosis factor receptor B11, D11, F11, H11 Hs.1314 NM_001243 TNFRSF8 CD30, D1S166E, Ki-1 superfamily, member 8 Zeta-chain (TCR) associated protein FLJ17670, FLJ17679, SRK, B12, D12, F12, H12 Hs.234569 NM_207519 ZAP70 kinase 70kDa STD, TZK, ZAP-70 Related products

Product Contents Cat. no.

EpiTect Methyl II DNA Reagents for the cleavage of methylated and 335452 Restriction Kit (12) unmethylated DNA for processing up to 12 DNA samples; 5x Restriction Digestion Buffer, Methylation-sensitive Enzyme A, Methylation-dependent Enzyme B

RT² SYBR® Green qPCR For 2 x 96 assays in 96-well plates; suitable for use 330500 Mastermixes(2)* with real-time cyclers that do not require a reference dye, including: Bio-Rad models CFX96, CFX384; Bio-Rad/MJ Research models Chromo4, DNA Engine Opticon 2; Roche LightCycler 480 (96-well and 384-well); all other cyclers

RT² SYBR Green ROX™ qPCR For 2 x 96 assays in 96-well plates; suitable for use 330520 Mastermixes(2)* with the following real-time cyclers: Applied Biosystems models 5700, 7000, 7300, 7500 [Standard and Fast], 7700, 7900HT 96-well block [Standard and Fast] and 384-well block, StepOnePlus; Eppendorf Mastercycler ep realplex models 2, 2S, 4, 4S; Stratagene models Mx3000P, Mx3005P, Mx4000

RT² SYBR Green Fluor qPCR For 2 x 96 assays in 96-well plates; suitable for use 330510 Mastermixes(2)* with the following real-time cyclers: Bio-Rad models iCycler, iQ5, MyiQ, MyiQ2

Accessories

EpiTect Methyl II Custom PCR For methylation analysis of customer-selected genes 335112 Array in a 96-well or 384-well plate format

EpiTect Methyl II Signature PCR For methylation analysis of 22 genes in a 96-well or 335212 Array (22) 384-well plate format

EpiTect Methyl II PCR Assay Laboratory-tested forward and reverse primers for 335002 (200) 200 x 25 µl reactions; 25 µl per primer; total volume: 200 µl

RT² PCR Array Loading 12 x 5 ml capacity, irradiation sterilized reservoirs 338162 Reservoir for convenient sample loading on PCR arrays

* Larger kit sizes available; please inquire. EpiTect Methyl II Signature PCR Array products are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

QIAGEN reserves the right to occasionally redesign individual assays on the EpiTect Methyl II PCR Arrays for improved performance. This revision history can be obtained by contacting technical support and providing the batch numbers from your arrays.

For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen. com or can be requested from QIAGEN Technical Services or your local distributor.

Purchaser agrees that use of this product and data therefrom is limited solely to the purchaser and for only the purchaser's own internal molecular biology research applications ("Permitted Use"), and shall not be re-sold or used for any other purposes (all of which are expressly prohibited), including without limitation diagnostic purposes, uses that could require regulatory approval for diagnostics from an agency of any government or regulatory entity anywhere in the world, diagnosis, prevention, or treatment of disease, and the right to perform commercial services of any kind, including without limitation, reporting the results of purchaser's activities, including without limitation, for a fee or other commercial consideration. Except for the Permitted Use, no rights, titles, or interests in or to any tangible or intangible property rights are conveyed or shall be deemed conveyed by implication, estoppel or otherwise. The performance characteristics of the product other than for the Permitted Use are unknown. Trademarks: QIAGEN®, EpiTect® (QIAGEN Group); MethylScreen™ (Orion Genomics, LLC); Roche®, LightCycler® (Roche Group); Applied Biosystems®, ViiA™, StepOnePlus™ (Applera Corporation or its subsidiaries); Eppendorf®, Mastercycler® (Eppendorf AG); Stratagene®, Mx3005P®, Mx3000P®, Mx4000® (Stratagene); Bio-Rad®, iCycler®, Chromo4™, CFX96™, DNA Engine Opticon®, CFX384™, iQ™, MyiQ™ (Bio-Rad Laboratories, Inc.); SYBR® (Molecular Probes, Inc.).

Purchaser agrees that use of this product and data therefrom is limited solely to the purchaser and for only the purchaser’s own internal molecular biology research applications (“Permitted Use”), and shall not be re-sold or used for any other purposes (all of which are expressly prohibited), including without limitation diagnostic purposes, uses that could require regulatory approval for diagnostics from an agency of any government or regulatory entity anywhere in the world, diagnosis, prevention, or treatment of disease, and the right to perform commercial services of any kind, including without limitation, reporting the results of purchaser’s activities, including without limitation, for a fee or other commercial consideration. Except for the Permitted Use, no rights, titles, or interests in or to any tangible or intangible property rights are conveyed or shall be deemed conveyed by implication, estoppel or otherwise. The performance characteristics of the product other than for the Permitted Use are unknown.

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Sample & Assay Technologies