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ZP4 Antibody Cat ZP4 Antibody Cat. No.: 30-535 ZP4 Antibody Antibody used in WB on recombinant human ZP4 protein. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human ZP4. TESTED APPLICATIONS: ELISA, WB ZP4 antibody can be used for detection of ZP4 by ELISA at 1:1562500. ZP4 antibody can be APPLICATIONS: used for detection of ZP4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL: 1) Cat. No. 1211 - HepG2 Cell Lysate PREDICTED MOLECULAR 49 kDa WEIGHT: September 29, 2021 1 https://www.prosci-inc.com/zp4-antibody-30-535.html Properties PURIFICATION: Antibody is purified by peptide affinity chromatography method. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose. CONCENTRATION: batch dependent For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZP4 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. Additional Info OFFICIAL SYMBOL: ZP4 ALTERNATE NAMES: ZP4, ZBP, ZP1, ZPB, Zp-4 ACCESSION NO.: NP_067009 PROTEIN GI NO.: 10863987 GENE ID: 57829 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix.The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane BACKGROUND: domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. September 29, 2021 2 https://www.prosci-inc.com/zp4-antibody-30-535.html REFERENCES: 1) Gregory, S.G., (2006) Nature 441 (7091), 315-321. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 29, 2021 3 https://www.prosci-inc.com/zp4-antibody-30-535.html.
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