8.3 the Process of Photosynthesis
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Light-Induced Psba Translation in Plants Is Triggered by Photosystem II Damage Via an Assembly-Linked Autoregulatory Circuit
Light-induced psbA translation in plants is triggered by photosystem II damage via an assembly-linked autoregulatory circuit Prakitchai Chotewutmontria and Alice Barkana,1 aInstitute of Molecular Biology, University of Oregon, Eugene, OR 97403 Edited by Krishna K. Niyogi, University of California, Berkeley, CA, and approved July 22, 2020 (received for review April 26, 2020) The D1 reaction center protein of photosystem II (PSII) is subject to mRNA to provide D1 for PSII repair remain obscure (13, 14). light-induced damage. Degradation of damaged D1 and its re- The consensus view in recent years has been that psbA transla- placement by nascent D1 are at the heart of a PSII repair cycle, tion for PSII repair is regulated at the elongation step (7, 15–17), without which photosynthesis is inhibited. In mature plant chloro- a view that arises primarily from experiments with the green alga plasts, light stimulates the recruitment of ribosomes specifically to Chlamydomonas reinhardtii (Chlamydomonas) (18). However, we psbA mRNA to provide nascent D1 for PSII repair and also triggers showed recently that regulated translation initiation makes a a global increase in translation elongation rate. The light-induced large contribution in plants (19). These experiments used ribo- signals that initiate these responses are unclear. We present action some profiling (ribo-seq) to monitor ribosome occupancy on spectrum and genetic data indicating that the light-induced re- cruitment of ribosomes to psbA mRNA is triggered by D1 photo- chloroplast open reading frames (ORFs) in maize and Arabi- damage, whereas the global stimulation of translation elongation dopsis upon shifting seedlings harboring mature chloroplasts is triggered by photosynthetic electron transport. -
Lecture 29 Spring 2007
Geol. 656 Isotope Geochemistry Lecture 29 Spring 2007 ISOTOPE FRACTIONATION IN THE BIOSPHERE INTRODUCTION As we noted, biological processes often involve large isotopic fractionations. Indeed, biological proc- esses are the most important cause of variations in the isotope composition of carbon, nitrogen, and sul- fur. For the most part, the largest fractionations occur during the initial production of organic matter by the so-called primary producers, or autotrophs. These include all plants and many kinds of bacteria. The most important means of production of organic matter is photosynthesis, but organic matter may also be produced by chemosynthesis, for example at mid-ocean ridge hydrothermal vents. Large frac- tions of both carbon and nitrogen occur during primary production. Additional fractionations also oc- cur in subsequent reactions and up through the food chain as hetrotrophs consume primary producers, but these are generally smaller. CARBON ISOTOPE FRACTIONATION DURING PHOTOSYNTHESIS The most important of process producing isotopic fractionation of carbon is photosynthesis. As we earlier noted, photosynthetic fractionation of carbon isotopes is primarily kinetic. The early work of Park and Epstein (1960) suggested fractionation occurred in several steps. Subsequent work has eluci- dated the fractionations involved in these steps, which we will consider in more detail here. For terrestrial plants (those utilizing atmospheric CO2), the first step is diffusion of CO2 into the boundary layer surrounding the leaf, through the stomata, and internally in the leaf. The average δ13C of various species of plants has been correlated with the stomatal conductance (Delucia et al., 1988), in- dicating that diffusion into the plant is indeed important in fractionating carbon isotopes. -
4.3 the Light-Dependent 4B, 9B Photosynthesis Indetail 9B Transfers Energy
DO NOT EDIT--Changes must be made through “File info” CorrectionKey=B 4.3 Photosynthesis in Detail 4B, 9B KEY CONCEPT Photosynthesis requires a series of chemical reactions. VOCABULARY MAIN IDEAS photosystem The first stage of photosynthesis captures and transfers energy. electron transport chain The second stage of photosynthesis uses energy from the first stage to make sugars. ATP synthase Calvin cycle Connect to Your World In a way, the sugar-producing cells in leaves are like tiny factories with assembly lines. 4B investigate and explain cellular processes, including In a factory, different workers with separate jobs have to work together to put homeostasis, energy conversions, together a finished product. Similarly, in photosynthesis many different chemical transport of molecules, and synthesis of new molecules and 9B reactions, enzymes, and ions work together in a precise order to make the sugars compare the reactants and products that are the finished product. of photosynthesis and cellular respiration in terms of energy and matter MaiN IDEA 4B, 9B The first stage of photosynthesis captures and transfers energy. In Section 2, you read a summary of photosynthesis. However, the process is much more involved than that general description might suggest. For exam- ple, during the light-dependent reactions, light energy is captured and trans- ferred in the thylakoid membranes by two groups of molecules called photosystems. The two photosystems are called photosystem I and photosys- tem II. Overview of the Light-Dependent Reactions FIGURE 3.1 The light-dependent The light-dependent reactions are the photo- part of photosynthesis. During reactions capture energy from sun- light and transfer energy through the light-dependent reactions, chlorophyll and other light-absorbing electrons. -
Chapter 3 the Title and Subtitle of This Chapter Convey a Dual Meaning
3.1. Introduction Chapter 3 The title and subtitle of this chapter convey a dual meaning. At first reading, the subtitle Photosynthetic Reaction might seem to indicate that the topic of the structure, function and organization of Centers: photosynthetic reaction centers is So little time, so much to do exceedingly complex and that there is simply insufficient time or space in this brief article to cover the details. While this is John H. Golbeck certainly the case, the subtitle is Department of Biochemistry additionally meant to convey the idea that there is precious little time after the and absorption of a photon to accomplish the Molecular Biology task of preserving the energy in the form of The Pennsylvania State University stable charge separation. University Park, PA 16802 USA The difficulty is there exists a fundamental physical limitation in the amount of time available so that a photochemically induced excited state can be utilized before the energy is invariably wasted. Indeed, the entire design philosophy of biological reaction centers is centered on overcoming this physical, rather than chemical or biological, limitation. In this chapter, I will outline the problem of conserving the free energy of light-induced charge separation by focusing on the following topics: 3.2. Definition of the problem: the need to stabilize a charge-separated state. 3.3. The bacterial reaction center: how the cofactors and proteins cope with this problem in a model system. 3.4. Review of Marcus theory: what governs the rate of electron transfer in proteins? 3.5. Photosystem II: a variation on a theme of the bacterial reaction center. -
Lecture Inhibition of Photosynthesis Inhibition at Photosystem I
1 Lecture Inhibition of Photosynthesis Inhibition at Photosystem I 1. General Information The popular misconception is that susceptible plants treated with these herbicides “starve to death” because they can no longer photosynthesize. In actuality, the plants die long before the food reserves are depleted. The photosynthetic inhibitors can be divided into two distinct groups, the inhibitors of Photosystem I and inhibitors of Photosystem II. Both of these groups work in the energy production step of photosynthesis, or the light reactions. Light is required as well as photosynthesis for these herbicides to kill susceptible plants. Herbicides that inhibit Photosystem I are considered to be contact herbicides and are often referred to as membrane disruptors. The end result is that cell membranes are rapidly destroyed resulting in leakage of cell contents into the intercellular spaces. These herbicides act as “electron interceptors” or “electron thieves” within Photosystem I of the light reaction of photosynthesis. They divert electrons from the normal electron transport sequence necessary in Photosystem I. This in turn inhibits photosynthesis. The membrane disruption occurs as a result of secondary responses. Herbicides that inhibit Photosystem I are represented by only one family, the bipyridyliums. See chemical structure shown under herbicide families. These molecules are cationic (positively charged) and are therefore highly water soluble. Their cationic properties also make them highly adsorbed to soil colloids resulting in no soil activity. 2. Mode of Action See Figure 7.1 (The electron transport chain in photosynthesis and the sites of action of herbicides that interfere with electron transfer in this chain (Q = electron acceptor; PQ = plastoquinone). -
Photosystem I-Based Applications for the Photo-Catalyzed Production of Hydrogen and Electricity
University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Doctoral Dissertations Graduate School 12-2014 Photosystem I-Based Applications for the Photo-catalyzed Production of Hydrogen and Electricity Rosemary Khuu Le University of Tennessee - Knoxville, [email protected] Follow this and additional works at: https://trace.tennessee.edu/utk_graddiss Part of the Biochemical and Biomolecular Engineering Commons Recommended Citation Le, Rosemary Khuu, "Photosystem I-Based Applications for the Photo-catalyzed Production of Hydrogen and Electricity. " PhD diss., University of Tennessee, 2014. https://trace.tennessee.edu/utk_graddiss/3146 This Dissertation is brought to you for free and open access by the Graduate School at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Doctoral Dissertations by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a dissertation written by Rosemary Khuu Le entitled "Photosystem I- Based Applications for the Photo-catalyzed Production of Hydrogen and Electricity." I have examined the final electronic copy of this dissertation for form and content and recommend that it be accepted in partial fulfillment of the equirr ements for the degree of Doctor of Philosophy, with a major in Chemical Engineering. Paul D. Frymier, Major Professor We have read this dissertation and recommend its acceptance: Eric T. Boder, Barry D. Bruce, Hugh M. O'Neill Accepted for the Council: Carolyn R. Hodges Vice Provost and Dean of the Graduate School (Original signatures are on file with official studentecor r ds.) Photosystem I-Based Applications for the Photo-catalyzed Production of Hydrogen and Electricity A Dissertation Presented for the Doctor of Philosophy Degree The University of Tennessee, Knoxville Rosemary Khuu Le December 2014 Copyright © 2014 by Rosemary Khuu Le All rights reserved. -
Lecture 7 - the Calvin Cycle and the Pentose Phosphate Pathway
Lecture 7 - The Calvin Cycle and the Pentose Phosphate Pathway Chem 454: Regulatory Mechanisms in Biochemistry University of Wisconsin-Eau Claire 1 Introduction The Calvin cycle Text The dark reactions of photosynthesis in green plants Reduces carbon from CO2 to hexose (C6H12O6) Requires ATP for free energy and NADPH as a reducing agent. 2 2 Introduction NADH versus Text NADPH 3 3 Introduction The Pentose Phosphate Pathway Used in all organisms Glucose is oxidized and decarboxylated to produce reduced NADPH Used for the synthesis and degradation of pentoses Shares reactions with the Calvin cycle 4 4 1. The Calvin Cycle Source of carbon is CO2 Text Takes place in the stroma of the chloroplasts Comprises three stages Fixation of CO2 by ribulose 1,5-bisphosphate to form two 3-phosphoglycerate molecules Reduction of 3-phosphoglycerate to produce hexose sugars Regeneration of ribulose 1,5-bisphosphate 5 5 1. Calvin Cycle Three stages 6 6 1.1 Stage I: Fixation Incorporation of CO2 into 3-phosphoglycerate 7 7 1.1 Stage I: Fixation Rubisco: Ribulose 1,5- bisphosphate carboxylase/ oxygenase 8 8 1.1 Stage I: Fixation Active site contains a divalent metal ion 9 9 1.2 Rubisco Oxygenase Activity Rubisco also catalyzes a wasteful oxygenase reaction: 10 10 1.3 State II: Formation of Hexoses Reactions similar to those of gluconeogenesis But they take place in the chloroplasts And use NADPH instead of NADH 11 11 1.3 State III: Regeneration of Ribulose 1,5-Bisphosphosphate Involves a sequence of transketolase and aldolase reactions. 12 12 1.3 State III: -
Answer Key Chapter 16: Standard Review Worksheet – + 1
Answer Key Chapter 16: Standard Review Worksheet – + 1. H3PO4(aq) + H2O(l) H2PO4 (aq) + H3O (aq) + + NH4 (aq) + H2O(l) NH3(aq) + H3O (aq) 2. When we say that acetic acid is a weak acid, we can take either of two points of view. Usually we say that acetic acid is a weak acid because it doesn’t ionize very much when dissolved in water. We say that not very many acetic acid molecules dissociate. However, we can describe this situation from another point of view. We could say that the reason acetic acid doesn’t dissociate much when we dissolve it in water is because the acetate ion (the conjugate base of acetic acid) is extremely effective at holding onto protons and specifically is better at holding onto protons than water is in attracting them. + – HC2H3O2 + H2O H3O + C2H3O2 Now what would happen if we had a source of free acetate ions (for example, sodium acetate) and placed them into water? Since acetate ion is better at attracting protons than is water, the acetate ions would pull protons out of water molecules, leaving hydroxide ions. That is, – – C2H3O2 + H2O HC2H3O2 + OH Since an increase in hydroxide ion concentration would take place in the solution, the solution would be basic. Because acetic acid is a weak acid, the acetate ion is a base in aqueous solution. – – 3. Since HCl, HNO3, and HClO4 are all strong acids, we know that their anions (Cl , NO3 , – and ClO4 ) must be very weak bases and that solutions of the sodium salts of these anions would not be basic. -
Can Ferredoxin and Ferredoxin NADP(H) Oxidoreductase Determine the Fate of Photosynthetic Electrons?
Send Orders for Reprints to [email protected] Current Protein and Peptide Science, 2014, 15, 385-393 385 The End of the Line: Can Ferredoxin and Ferredoxin NADP(H) Oxidoreductase Determine the Fate of Photosynthetic Electrons? Tatjana Goss and Guy Hanke* Department of Plant Physiology, Faculty of Biology and Chemistry, University of Osnabrück,11 Barbara Strasse, Osnabrueck, DE-49076, Germany Abstract: At the end of the linear photosynthetic electron transfer (PET) chain, the small soluble protein ferredoxin (Fd) transfers electrons to Fd:NADP(H) oxidoreductase (FNR), which can then reduce NADP+ to support C assimilation. In addition to this linear electron flow (LEF), Fd is also thought to mediate electron flow back to the membrane complexes by different cyclic electron flow (CEF) pathways: either antimycin A sensitive, NAD(P)H complex dependent, or through FNR located at the cytochrome b6f complex. Both Fd and FNR are present in higher plant genomes as multiple gene cop- ies, and it is now known that specific Fd iso-proteins can promote CEF. In addition, FNR iso-proteins vary in their ability to dynamically interact with thylakoid membrane complexes, and it has been suggested that this may also play a role in CEF. We will highlight work on the different Fd-isoproteins and FNR-membrane association found in the bundle sheath (BSC) and mesophyll (MC) cell chloroplasts of the C4 plant maize. These two cell types perform predominantly CEF and LEF, and the properties and activities of Fd and FNR in the BSC and MC are therefore specialized for CEF and LEF re- spectively. -
Thylakoid Membrane Architecture in Cyanobacteria
THYLAKOID MEMBRANE ARCHITECTURE IN CYANOBACTERIA Dissertation zur Erlangung des Grades eines Doktors der Naturwissenschaften an der Fakultät für Biologie der Ludwig-Maximilians-Universität München vorgelegt von Anna Margareta Rast München, 03. Mai 2018 1. Gutachter: Prof. Dr. Jörg Nickelsen 2. Gutachter: Prof. Dr. Andreas Klingl Tag der Abgabe: 03.05.2018 Tag der mündlichen Prüfung: 22.06.2018 2 TABLE OF CONTENT TABLE OF CONTENT SUMMARY 5 ZUSAMMENFASSUNG 7 1 INTRODUCTION 9 1.1 Evolution of oxygenic photosynthesis 9 1.2 Thylakoid structure 10 1.2.1 Thylakoid structure in plastids 10 1.2.2 Thylakoid structure in cyanobacteria 11 1.3 Thylakoid membrane shaping factors 13 1.4 Photosynthesis in cyanobacteria 17 1.4.1 Electron transport chain 17 1.4.2 Light harvesting 19 1.4.3 Lateral heterogeneity 20 1.5 PSII biogenesis and repair in cyanobacteria 21 1.6 Cryo-electron tomography 23 2 AIMS OF THIS WORK 27 3 RESULTS 28 3.1 The role of Slr0151, a tetratricopeptide repeat protein from Synechocystis sp. PCC 6803, during photosystem II assembly and repair 28 3.2 Thylakoid membrane architecture in Synechocystis depends on CurT, a homolog of the granal CURVATURE THYLAKOID1 proteins 41 3.3 In situ cryo-electron tomography of cyanobacterial thylakoid convergence zones reveals a biogenic membrane connecting thylakoids to the plasma membrane. 65 4 DISCUSSION 86 4.1 Structural and photosystem II specific role of Slr0151 and CurT 86 4.1.1 The role of Slr0151 86 4.1.2 The role of CurT 88 4.1.3 Connection between Slr0151 and CurT via phosphorylation? 90 4.2 The thylapse – a contact area between thylakoids and plasma membrane 91 4.2.1 Factors possibly involved in thylapse formation 93 4.2.2 Membrane contact – a common feature 94 4.3 Lateral heterogeneity in cyanobacteria 95 4.4 Conclusions and future perspectives 97 3 TABLE OF CONTENT 5 REFERENCES 99 6 APPENDIX 112 6.1 Biogenesis of thylakoid membranes 112 6.2 Supplementary Material - The role of Slr0151, a tetratricopeptide repeat protein from Synechocystis sp. -
BIOLOGICAL SCIENCE FIFTH EDITION Freeman Quillin Allison 10
BIOLOGICAL SCIENCE FIFTH EDITION Freeman Quillin Allison 10 Lecture Presentation by Cindy S. Malone, PhD, California State University Northridge © 2014 Pearson Education, Inc. Roadmap 10 In this chapter you will learn how Photosynthesis links life to the power of the Sun by previewing by examining Conversion of light How photosynthetic pigments energy into chemical capture light energy 10.2 energy 10.1 then looking closer at Energy flow and ATP Photosystem II production10.3 Photosystem I and exploring CO2 fixation and reduction to The Calvin cycle form sugars 10.4 © 2014 Pearson Education, Inc. ▪ Photosynthesis – Is the process of using sunlight to produce carbohydrate – Requires sunlight, carbon dioxide, and water – Produces oxygen as a by-product ▪ The overall reaction when glucose is the carbohydrate: 6 CO2 6 H2O light energy C6H12O6 6 O2 © 2014 Pearson Education, Inc. ▪ Photosynthesis contrasts with cellular respiration – Photosynthesis is endergonic – Reduces CO2 to sugar – Cellular respiration is exergonic – Oxidizes sugar to CO2 Electrons are Electrons are pulled __________; pulled _______________; C is _________ O is _________ Potential energy increases 6 CO2 6 H2O Input of 6 O2 (carbon dioxide) (water) energy Glucose (oxygen) © 2014 Pearson Education, Inc. ▪ Light-dependent reactions – Produce O2 from H2O ▪ Calvin cycle reactions – Produce sugar from CO2 ▪ The reactions are linked by electrons – Released in the light-dependent reactions – When water is split to form oxygen gas – Then transferred to the electron carrier NADP+, forming NADPH © 2014 Pearson Education, Inc. ▪ The Calvin cycle Figure 10.2 then uses Sunlight (Light – These electrons energy) – The potential Light- energy in ATP capturing reactions – To reduce CO2 to (Chemical make sugars energy) Calvin cycle (Chemical energy) © 2014 Pearson Education, Inc. -
AP Biology-00001310.Cdr
® INTERNATIONAL ACADEMY OF SCIENCE Acellus AP Biology AP Biology Course Curriculum Unit 1 - Evolution Drives the Diversity and Unity of Life 46 Photosystems 1 Intro to AP Biology 47 Photophosphorylation 2 Nature of Science 48 Carbon Fixation (or Calvin Cycle) 3 Evidence for Evolution 49 Putting It Together - Photosynthesis and Respiration 4 Natural Selection - Descent with Modification 50 Feedback Mechanisms 5 Hardy - Weinberg Theorem 51 Cell Communication 6 Hardy - Weinberg Equilibrium Unit 6 - The Cell Cycle 7 Biological Evolution 52 Why Do Cells Divide? 8 Phylogeny - Evolutionary History 53 Origin of the Cell Cycle 9 Modern Synthesis Theory of Evolution 54 Chromosome Structure Unit 2 - Water Potential 55 Phases of the Cycle 10 Abiogenesis 56 Lab: Cell Division - Part I 11 Properties of Water 57 Lab: Cell Division - Part II 12 Organic Molecules 58 Variances in the Cell Cycle 13 Origin of Cells 59 Control of the Cell Cycle 14 Endosymbiosis 60 Uncontrolled Cell Cycle 15 Characteristics of Life 61 Lab: Cell Division - Part III 16 Cell Membranes - Structure Unit 7 - Mitosis and Meiosis 17 Selective Permeability 62 Two Types of Cell Reproduction 18 Diffusion and Cell Size 63 Meiosis Overview 19 Water Potential - Concentration Gradient 64 The Phases of Meiosis 20 Lab: Water Potential 65 Meiosis and Genetic Variation Unit 3 - Cell Structure 66 Lab: Cell Division - Part IV 21 Basic Cell Structure 67 Lab: Cell Division - Part V 22 Prokaryotes 68 Meiosis and Gamete Formation 23 Eukaryotes Unit 8 - History of Genetics 24 Mitochondria and Chloroplasts