Revision 1 C 0 2 - t METTL14 (D8K8W) Rabbit mAb a e r o t S Orders: 877-616-CELL (2355)
[email protected] Support: 877-678-TECH (8324) 4 0 1 Web:
[email protected] 1 www.cellsignal.com 5 # 3 Trask Lane Danvers Massachusetts 01923 USA For Research Use Only. Not For Use In Diagnostic Procedures. Applications: Reactivity: Sensitivity: MW (kDa): Source/Isotype: UniProt ID: Entrez-Gene Id: WB H M R Mk Endogenous 65 Rabbit IgG Q9HCE5 57721 Product Usage Information 5. Ping, X.L. et al. (2014) Cell Res 24, 177-89. 6. Lin, S. et al. (2016) Mol Cell 62, 335-45. Application Dilution 7. Jin, D.I. et al. (2012) Cancer Sci 103, 2102-9. 8. Jo, H.J. et al. (2013) J Gastroenterol 48, 1271-82. Western Blotting 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. Specificity / Sensitivity METTL14 (D8K8W) Rabbit mAb recognizes endogenous levels of total METL14 protein. Species Reactivity: Human, Mouse, Rat, Monkey Species predicted to react based on 100% sequence homology: Chicken, Xenopus Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro130 of human METTL14 protein. Background Methyltransferase-like protein 3 (METTL3) and methytransferase-like protein 14 (METTL14) are the two catalytic subunits of an N6-methyltransferase complex that methylates adenosine residues in RNA (1). Methylation of adenosine residues regulates mRNA splicing, processing, translation efficiency, editing and stability, in addition to regulating primary miRNA processing, and is critical for proper regulation of the circadian clock, embryonic stem cell self-renewal, immune tolerance, response to various stimuli, meiosis and mouse fertility (2,3).