Catalog # Aliquot Size

U232-911-05 3 x 5 nmol U232-911-20 3 x 20 nmol U232-911-50 3 x 50 nmol

UBE2O siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # U232-911

Lot # Z2109-30

Specificity Formulation UBE2O siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human UBE2O expression. shipped at room temperature.

Product Description Reconstitution Protocol UBE2O siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human UBE2O mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The Resuspend the siRNA in 50 µl of DEPC-treated water accession number is BC022237. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot E2-230K, FLJ12878, KIAA1734 1x stock solutions for storage.

Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or UBE2N (UBC13) U231-30H below -20oC. After resuspension, siRNA stock solutions ≥2 UBE2O Protein U232-30G µM can undergo up to 50 freeze-thaw cycles without UBE2R1 Protein U234-30H significant degradation. For long-term storage, it is UBE2R2 (UBC3B) Protein U235-30H recommended that the siRNA is stored at -70oC. For most UBE2U Protein U237-30G favorable performance, avoid repeated handling and UBE2V2 Protein U239-30H multiple freeze/thaw cycles. UBE2W (UBC16) Protein U240-30G UBE2Z (USE1) Protein U241-31H Scientific Background UBE2O or -conjugating enzyme E2O is a part of the ubiquitin-mediated protein degradation pathway in UBE2O siRNA Set I siRNA duplexes targeted against three exon regions which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes Catalog # U232-911 with ubiquitin. HIV-1 Tat has been identified to have a Lot # Z2109-30 physical interaction with ubiquitin-conjugating enzyme Packaging Specifications 2.5 nmol/tube for 3 x 5nmol E2O (UBE2O) in human HEK293 and Jurkat cell lines by using affinity tagging and purification mass spectrometry Format Lyophilized powder Stability 1yr at -70oC from date of shipment analyses (1). Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized References and resuspended siRNAs are stored at 1. Rumbaugh JA. et.al: HIV immune complexes prevent or below -20oC. After resuspension, excitotoxicity by interaction with NMDA receptors. siRNA stock solutions ≥2 µ M can Neurobiol Dis, 2012 Aug 25. undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.