Product Datasheet Anti-BASP1 antibody ab101855

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Overview

Product name Anti-BASP1 antibody Description Rabbit polyclonal to BASP1 Tested applications WB, IHC-P, IHC-Fr, ICC/IF Species reactivity Reacts with: Mouse, Rat, Human

Immunogen A synthetic peptide from the N terminal region of Mouse BASP1 conjugated to an immunogenic carrier .

Positive control This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: SKNSH. Properties

Form Liquid Storage instructions Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. Storage buffer Preservative: None Constituents: Whole serum, 1X PBS Purity Whole antiserum Clonality Polyclonal Isotype IgG Research Areas Neuroscience Cell Type Marker Neuron marker Growth Cone Neuroscience Neurology process Neural Signal Transduction Epigenetics and Nuclear Signaling Transcription Other factors Applications

Our Abpromise guarantee covers the use of ab101855 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes WB 1/300 - 1/2000. Predicted molecular weight: 23 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. IHC-P 1/300 - 1/2000. Antigen retrieval is recommended. IHC-Fr 1/300 - 1/2000. ICC/IF Use a concentration of 5 µg/ml. Target

Relevance BASP1 is a brain abundant membrane bound protein with several transient phosphorylation sites and PEST motifs. BASP1 binds calmodulin, is phosphorylated by protein kinase C and undergoes N-terminal myristoylation. BASP1 is expressed specifically in nervous tissue. Intercellular signaling and nerve outgrowth are influenced by a number of expressed at nerve endings and synapses of which BASP1 is one.

http://www.abcam.com/BASP1-antibody-ab101855.html 1 Product Datasheet Cellular localization Cell membrane; Lipid-anchor. Cell projection; growth cone. Note: Associated with the membranes of growth cones that form the tips of elongating axons. Database links : 10409 Human Entrez Gene: 70350 Mouse Entrez Gene: 64160 Rat Omim: 605940 Human SwissProt: P80723 Human SwissProt: Q91XV3 Mouse SwissProt: Q05175 Rat Unigene: 201641 Human Unigene: 29586 Mouse Unigene: 37779 Rat Alternative names 22 kDa neuronal tissue enriched acidic protein antibody BASP 1 antibody BASP1 protein antibody Brain abundant membrane attached signal protein 1 antibody Brain Abundant Signal Protein Membrane Attached 1 antibody Brain Acid Soluble Protein 1 antibody CAP 23 antibody CAP23 antibody MGC8555 antibody NAP 22 antibody NAP22 antibody Neuronal axonal membrane protein NAP 22 antibody Neuronal tissue enriched acidic protein antibody Anti-BASP1 antibody images

ICC/IF image of ab101855 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101855 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to Immunocytochemistry/ label plasma membranes (red) at a 1/200 Immunofluorescence - Anti-BASP1 dilution for 1h. DAPI was used to stain the cell antibody (ab101855) nuclei (blue) at a concentration of 1.43µM.

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Guarantee only valid for products bought direct from Abcam or one of our authorized distributors **Regional variations to our Abpromise may apply to the following countries: China, Korea, Singapore, Malaysia, Taiwan and Thailand, which operate a 120 day guarantee. Please contact your regional office for further details Antibodies tested only in recombinant samples are not covered for use on endogenous samples. Please check the datasheet for information on tested species "Predicted to react" information on datasheets is based on and provided for reference only "Predicted to react" species are not guaranteed, as they have not been tested Fast Track products are not covered under the Abpromise. Please see Fast Track terms and condition for additional information Abcam biochemicals are novel compounds and we have not tested their biological activity in house. Please use the literature to identify how to use these products effectively. If you require further assistance please contact the scientific support team

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