21.10.2014TERF2 antibody (pAb)

Rabbit Anti -Human Telomeric repeat binding factor 2 (TRBF2, TRF2)

Instruction Manual

Catalog Number PK-AB718-7727 Synonyms Telomeric repeat binding factor 2, Telomeric DNA-binding , Telomeric repeat-binding factor 2, TRBF2, TRF2, TTAGGG repeat-binding factor 2 Description The telomeric repeat binding factor family (TERF1 and TERF2) plays a key role in cellular immortalization and cellular senescence. TERF2, as a component of the complex, is present at in of the . TERF2 is a second negative regulator of telomere length and binds as a dimer to TTAGGG repeats at ends of (telomeres), where it blocks inappropriate activation of the ATM/ pathway. TERF1 negatively regulates telomere elongation, while TERF2 protects the ends by inhibiting end-to- end fusions. Down-regulation of TRF expression in tumor cells may contribute to cell immortalization and malignant progression. Quantity 100 µg Source / Host Rabbit Immunogen TERF2 antibody was raised against a 17 amino peptide near the amino terminus of human TERF2. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human Specificity TERF2 antibody is human specific. Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. TERF2 antibody can be used for detection of TERF2 by Western blot at 1 - 2 µg/ml. Images Available upon request. References Broccoli D, Smogorzewska A, Chong L, et al. Human telomeres contain two distinct Myb-related , TRF1 and TRF2. Nat. Genet. 1997; 17:231-5. Bilaud T, Brun C, Ancelin K, et al. Telomeric localization of TRF2, a novel human telobox protein. Nat. Genet. 1997; 17:236-9. van Steensel B and Smogorzewska A. TRF2 protects human telomeres from end-to-end fusions. Cell 1998; 92:401-13. Matsutani N, Yokozaki H, Tahara E, et al. Expression of TRF1 and 2 and TRF1-interacting nuclear protein 2 in human gastric carcinomas. Int. J. Oncol. 2001; 19:507-12. Images Available upon request. Related Products Cat. No. PK-AB718-7727P - TERF2 Peptide Cat. No. PK-AB718-1210 - 293 Cell Lysate

FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014