TNNT1 Antibody Cat. No.: 25-740
TNNT1 Antibody
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human, Mouse
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human TNNT1.
TESTED APPLICATIONS: ELISA, WB
TNNT1 antibody can be used for detection of TNNT1 by ELISA at 1:312500. TNNT1 APPLICATIONS: antibody can be used for detection of TNNT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
POSITIVE CONTROL: 1) Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate
PREDICTED MOLECULAR 33 kDa WEIGHT:
Properties
PURIFICATION: Antibody is purified by peptide affinity chromatography method.
CLONALITY: Polyclonal
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
September 25, 2021 1 https://www.prosci-inc.com/tnnt1-antibody-25-740.html Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.
CONCENTRATION: batch dependent
For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: TNNT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
OFFICIAL SYMBOL: TNNT1
ALTERNATE NAMES: TNNT1, ANM, MGC104241, TNT, NEM5, STNT, TNTS
ACCESSION NO.: NP_003274
PROTEIN GI NO.: 187173288
GENE ID: 7138
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
TNNT1 is a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year.This gene BACKGROUND: encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene.
REFERENCES: 1) Witt, S.H., (2005) J. Mol. Biol. 350 (4), 713-722.
ANTIBODIES FOR RESEARCH USE ONLY.
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