DOCSLIB.ORG

Clinical Immunodiagnostics

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Clinical Immunodiagnostics © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION ClinicalNOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC ImmunodiagnosticsNOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION Laboratory Principles and Practices © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION Ian C. Clift, PhD MLS(ASCP)CM © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION IndianaNOT University FOR SALE OR DISTRIBUTION Program Director, Clinical Laboratory Science Indiana University South Bend South Bend, Indiana © Jones & BartlettScientific Learning, Consultant, BiomedicalLLC Associates © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning,© Jones LLC & Bartlett Learning LLC, an Ascend Learning© Jones Company. & NOT Bartlett FOR SALE Learning, OR DISTRIBUTION. LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION 9781284173017_FMxx_Clift.indd 1 2020-01-03 2:46 PM © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC World Headquarters Jones & Bartlett LearningNOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION 5 Wall Street Burlington, MA 01803 978-443-5000 [email protected] www.jblearning.com © JonesJones & Bartlett & LearningBartlett books Learning,and products are availableLLC through most bookstores and online© Jones booksellers. & To Bartlettcontact Jones &Learning, Bartlett LLC NOTLearning FOR directly, SALE call 800-832-0034, OR DISTRIBUTION fax 978-443-8000, or visit our website, www.jblearning.com.NOT FOR SALE OR DISTRIBUTION Substantial discounts on bulk quantities of Jones & Bartlett Learning publications are available to corporations, professional associations, and other qualified organizations. For details and specific discount information, contact the special sales department at Jones & Bartlett Learning via the above contact information or send an email to [email protected]. © Jones & BartlettCopyright Learning, © 2021 by Jones &LLC Bartlett Learning, LLC, an Ascend Learning© Jones Company & Bartlett Learning, LLC NOT FOR SALEAll ORrights reserved.DISTRIBUTION No part of the material protected by this copyrightNOT may be reproducedFOR SALE or utilized OR in any DISTRIBUTION form, electronic or mechanical, including photocopying, recording, or by any information storage and retrieval system, without written permission from the copyright owner. The content, statements, views, and opinions herein are the sole expression of the respective authors and not that of Jones & Bartlett Learning, LLC. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not constitute or imply its endorsement or recommendation by Jones & Bartlett Learning, LLC and such reference shall not be used for advertising or product endorsement purposes. All trademarks displayed are the trademarks of the parties noted herein. Clinical Immunodiagnostics: Laboratory© Jones Principles & Bartlettand Practices is Learning, an independent publication LLC and has not been authorized, sponsored,© Jones or otherwise & Bartlett Learning, LLC approved by the ownersNOT of the FORtrademarks SALE or service OR marks DISTRIBUTION referenced in this product. NOT FOR SALE OR DISTRIBUTION There may be images in this book that feature models; these models do not necessarily endorse, represent, or participate in the activities represented in the images. Any screenshots in this product are for educational and instructive purposes only. Any individuals and scenarios featured in the case studies throughout this product may be real or fictitious, but are used for instructional purposes only. Production Credits © VP,Jones Product Management:& Bartlett Amanda Learning, Martin LLC Cover Design: Theresa© JonesManley & Bartlett Learning, LLC Director of Product Management: Cathy L. Esperti Text Design: Kristin E. Parker NOTProduct FOR Assistant: SALE Melina LeonOR DISTRIBUTION Senior Media DevelopmentNOT Editor:FOR Troy SALE Liston OR DISTRIBUTION Product Specialist: Andrew LaBelle Rights Specialist: Rebecca Damon Project Specialist: Nora Menzi Rights Specialist: Liz Kincaid Digital Products Manager: Jessica deMartin Cover Image (Title Page, Part Opener, Chapter Opener): Marketing Manager: Suzy Balk © Science photo/Shutterstock VP, Manufacturing and Inventory Control: Therese Connell Printing and Binding: LSC Communications © Jones & BartlettComposition Learning, and Project Management: LLC Exela Technologies © Jones & Bartlett Learning, LLC NOT FOR SALELibrary OR of DISTRIBUTIONCongress Cataloging-in-Publication Data NOT FOR SALE OR DISTRIBUTION Names: Clift, Ian C., author. Title: Clinical immunodiagnostics : laboratory principles and practices / Ian C. Clift. Description: First edition. | Burlington, MA : Jones & Bartlett Learning, [2021] | Includes bibliographical references and index. | Summary: “The purpose of this project is to provide a contemporary guide to the diagnostic principles and practices of immunology and serology in the clinical laboratory. This book will focus on an entry level understanding of clinical immunology, serology, and immunodiagnostics for use in the diagnostic© Jones clinical lab, & itBartlett will also touch Learning, on conceptual overlaps LLC with blood banking (immunohematology),© Jones transfusion & Bartlett Learning, LLC medicine, infectiousNOT disease, FOR cellular SALEphenotyping, OR and DISTRIBUTIONoncology as they pertain to immunology-based clinical diagnostics.NOT FOR The book SALE OR DISTRIBUTION will be selective in its coverage; emphasizing entry-level knowledge and contemporary practical approaches. Content will also cover important historical and emerging concepts in the field”— Provided by publisher. Identifiers: LCCN 2019033935 | ISBN 9781284173017 (paperback) Subjects: MESH: Immunologic Tests Classification: LCC RB46.5 | NLM QY 250 | DDC 616.07/56—dc23 © JonesLC record &available Bartlett at https://lccn.loc.gov/2019033935 Learning, LLC © Jones & Bartlett Learning, LLC NOT6048 FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION Printed in the United States of America 24 23 22 21 20 10 9 8 7 6 5 4 3 2 1 © Jones & Bartlett Learning,© Jones LLC & Bartlett Learning LLC, an Ascend Learning© Jones Company. & NOT Bartlett FOR SALE Learning, OR DISTRIBUTION. LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION 9781284173017_FMxx_Clift.indd 2 2020-01-03 2:46 PM © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION To my family and all forward-looking laboratory professionals © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning,© Jones LLC & Bartlett Learning LLC, an Ascend Learning© Jones Company. & NOT Bartlett FOR SALE Learning, OR DISTRIBUTION. LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION 9781284173017_FMxx_Clift.indd 3 2020-01-03 2:46 PM © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning, LLC © Jones & Bartlett Learning, LLC NOT FOR SALE OR DISTRIBUTION NOT FOR SALE OR DISTRIBUTION © Jones & Bartlett Learning,
Load more

Recommended publications
  • CCR7 Antibody (Y59) NB110-55680
    Product Datasheet CCR7 Antibody (Y59) NB110-55680 Unit Size: 0.1 ml Store at -20C. Avoid freeze-thaw cycles. Publications: 9 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/NB110-55680 Updated 5/28/2019 v.20.1 Earn rewards for product reviews and publications. Submit a publication at www.novusbio.com/publications Submit a review at www.novusbio.com/reviews/destination/NB110-55680 Page 1 of 6 v.20.1 Updated 5/28/2019 NB110-55680 CCR7 Antibody (Y59) Product Information Unit Size 0.1 ml Concentration This product is unpurified. The exact concentration of antibody is not quantifiable. Storage Store at -20C. Avoid freeze-thaw cycles. Clonality Monoclonal Clone Y59 Preservative 0.01% Sodium Azide Isotype IgG Purity Tissue culture supernatant Buffer 49% PBS, 0.05% BSA and 50% Glycerol Target Molecular Weight 45 kDa Product Description Host Rabbit Gene ID 1236 Gene Symbol CCR7 Species Human, Mouse, Rat, Monkey Reactivity Notes May cross reacts with Rhesus monkey. Specificity/Sensitivity The antibody does not cross-react with other G-protein coupled receptor 1 family members. Immunogen A synthetic peptide corresponding to residues in N-terminal extracellular domain of human CKR7 was used as immunogen. Notes Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. Product Application Details Applications Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry- Paraffin, Immunoprecipitation, Flow Cytometry (Negative) Recommended Dilutions Western Blot 1:1000-10000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:250, Immunoprecipitation 1:10, Immunohistochemistry-Paraffin 1:250, Immunohistochemistry-Frozen 1:250, Flow Cytometry (Negative) Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation.
    [Show full text]
  • Memoranaurns by the Participants in Signes Par Les Partici- I the Meeting
    Memoranda are state- Les Memorandums ments concerning the exposent les conclu- /e , conclusions or recom- sions et recomman- M e mmooranrantedaa mendations of certain dations de certaines / a t w / /WHO scientific meet- /reunions scientifiques ings; they are signed de /'OMS; ils sont Memoranaurns by the participants in signes par les partici- I the meeting. pants a ces reunions. Bulletin ofthe World Health Organization, 62 (2): 217-227 (1984) © World Health Organization 1984 Immunodiagnosis simplified: Memorandum from a WHO Meeting* Technologies suitable for the development ofsimplified immunodiagnostic tests were reviewed by a Working Group of the WHO Advisory Committee on Medical Research in Geneva in June 1983. They included agglutination tests and use ofartificialparticles coated with immunoglobulins, direct visual detection of antigen-antibody reactions, enzyme- immunoassays, and immunofluorescence and fluoroimmunoassays. The use of mono- clonal antibodies,in immunodiagnosis and of DNA /RNA probes to identify viruses was also discussed in detail. The needfor applicability of these tests at three levels, i.e., field conditions (or primary health care level), local laboratories, and central laboratories, was discussed and their use at thefield level was emphasized. Classical serological techniques have been used for All these tests can be carried out in laboratories that a long time for diagnostic purposes, e.g., for con- are equipped with basic instruments as well as special- firmation of clinical diagnoses, epidemiological ized apparatus (e.g., gamma counters for RIA, ultra- studies, testing of blood donors, etc. Some of these violet microscopes for IMF, etc.), which are usually techniques have been standardized to a high degree of available only in the larger, central laboratories.
    [Show full text]
  • Cytokine Immunocytochemistry Fax (65) 860-1590 Fax (49) 40 531 58 92 Fax (81) 3 541-381-55 E-Mail: [email protected]
    PharMingen International United States Canada PharMingen Europe Japan PharMingen Canada Tel 619-812-8800 Asia Pacific Becton Dickinson GmbH Nippon Becton Dickinson Toll-Free 1-888-259-0187 Orders 1-800-848-6227 BD Singapore HQ PharMingen Europe Company Ltd. Tel 905-542-8028 Tech Service 1-800-825-5832 Tel (65) 860-1478 Tel (49) 40 532 84 48 0 Tel (81) 3 541-382-51 Fax 905-542-9391 Fax 619-812-8888 Cytokine Immunocytochemistry Fax (65) 860-1590 Fax (49) 40 531 58 92 Fax (81) 3 541-381-55 e-mail: [email protected] http://www.pharmingen.com Africa Germany Latin/South America Spain Becton Dickinson Worldwide Inc. Becton Dickinson GmbH BDIS (USA) Becton Dickinson S.A. Reagents and Techniques Tel (254) 2 449 608 HQ PharMingen Europe Tel (408) 954-2157 Tel (34) 91 848 8100 Fax (254) 2 449 619 Tel (49) 40 532 84 48 0 Fax (408) 526-1804 Fax (34) 91 848 8105 Fax (49) 40 531 58 92 Orders for Microscopic Analysis of Cytokine-Producing Cells Australia Malaysia Tel (34) 91 848 8182 Becton Dickinson Pty Ltd Greece Becton Dickinson Sdn Bhd Fax (34) 91 848 8104 Tel (612) 9978-6800 Becton Dickinson Hellas S.A. Tel (03) 7571323 Fax (612) 9978-6850 Tel (30) 1 9407741 Fax (03) 7571153 Sweden Fax (30) 1 9407740 Becton Dickinson AB Austria Mexico Tel (46) 8 775 51 00 Becton Dickinson GmbH Hong Kong Becton Dickinson de Mexico Fax (46) 8 645 08 08 HQ PharMingen Europe Becton Dickinson Asia Ltd Tel (52-5) 237-12-98 Tel (49) 40 532 84 48 0 Tel (852) 2572-8668 Fax (52-5) 237-12-93 Switzerland Fax (49) 40 531 58 92 Fax (852) 2520-1837 Becton Dickinson GmbH Middle East HQ PharMingen Europe Belgium Hungary Becton Dickinson Tel (41) 06 1-385 4422 Becton Dickinson Benelux N.V.
    [Show full text]
  • AG 39: Immunofluorescence Assays (PDF)
    ibidi Application Guide Immunofluorescence Assays The Principle of Immunofluorescence Immunofluorescence Applied: Assays . 2 Experimental Examples . 13 Rat Hippocampal Neuron and Astrocyte Staining 14 Immunofluorescence Staining: Visualization of Endothelial Cell Junctions . 13 A Typical Workflow . 3 Immunostaining of Rat Dorsal Root Ganglionic Experiment Planning and Sample Preparation . 4 Cells and Schwann Cells . 13 Sample Fixation . 4 Adherens Junctions and Actin Cytoskeleton of Cell Permeabilization . 5 HUVECs Under Flow . 14 Blocking . 5 Mitochondria Staining of MDCK cells . 14 Primary Antibody Incubation . 5 Focal Adhesions of Differentiated Mouse Fibroblasts on an Elastic Surface . 15 Secondary Antibody Incubation . 6 Counterstain and Mounting . 7 Microscopy . 7 Troubleshooting . 8 Selected Publications Immunofluorescence C. Xu, et al. NPTX2 promotes colorectal cancer growth and liver With the ibidi Chambers . 9 metastasis by the activation of the canonical Wnt/beta-catenin pathway via FZD6. Cell Death & Disease, 2019, 10.1038/s41419- Comparison of Immunocytochemistry Protocols . 10 019-1467-7 Chambered Coverslips . 11 read abstract Kobayashi, T., et al. Principles of early human development and Channel Slides . 11 germ cell program from conserved model systems. Nature, Chamber Slides . 12 2017, 10.1038/nature22812 read abstract H. Tada et al. Porphyromonas gingivalis Gingipain-Dependently Enhances IL-33 Production in Human Gingival Epithelial Cells. PloS one, 2016, 10.1371/journal.pone.0152794 read abstract N. J. Foy, M. Akhrymuk, A. V. Shustov, E. I. Frolova and I. Frolov. Hypervariable Domain of Nonstructural Protein nsP3 of Venezuelan Equine Encephalitis Virus Determines Cell-Specific Mode of Virus Replication. Journal of Virology, 2013, 10.1128/ jvi.00720-13 read abstract .com The Principle of Immunofluorescence Assays Immunofluorescence (IF) is a powerful approach for getting insight into cellular structures and processes using microscopy .
    [Show full text]
  • 116588NCJRS.Pdf
    If you have issues viewing or accessing this file contact us at NCJRS.gov. " "'- u.s. Department of Justice Federal Bureau of Investigation PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON FORENSIC IMMUNOLOGY FBI ACADEMY QUANTICO, VIRGINIA JUNE 23-26, 1986 Proceedings of the International Symposium on Forensic Immunology co 00 L() \0 u, r-l O§ r-l Ql C. If) 05~ Ql 0- 0 ~ o~ Ql E C/) Ql >. c:0 If) C/) ~ * ....,a: () a: z Ql '*02 Ql iii :5 ...... ....,::> a Ql {ij "...:0- Ql c: !!lc: °E ::>;: B 00 {ij c: 0 ~I::> z~ "e CD ~~ :5 .... ~o- S 1::C: Lt .~ Host Laboratory Division Federal Bureau of Investigation June 23-26, 1986 Forensic Science Research and Training Center FBI Academy Quantico, Virginia NOTICE This publication was prepared by the U. S. Government. Neither the U. S. Government nor the U. S. Department of Justice nor any of their employees makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness or usefulness of any information, apparatus, product or process disclosed, or represents that in use would not infringe privately owned rights. Reference herein to any specific commercial product, process or service by trade name, mark, manufacturer or otherwise does not necessarily constitute or imply its endorsement, recommendation or favoring by the U. S. Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the U. S. Government or any agency thereof. Published by: The Laboratory Division Roger T. Castonguay Assistant Director in Charge Federal Bureau of Investigation U.
    [Show full text]
  • Laboratory Analysis of the Immune Response 859
    Chapter 20 | Laboratory Analysis of the Immune Response 859 Chapter 20 Laboratory Analysis of the Immune Response Figure 20.1 Lab-on-a-chip technology allows immunological assays to be miniaturized so tests can be done rapidly with minimum quantities of expensive reagents. The chips contain tiny flow tubes to allow movement of fluids by capillary action, reactions sites with embedded reagents, and data output through electronic sensors. (credit: modification of work by Maggie Bartlett, NHGRI) Chapter Outline 20.1 Polyclonal and Monoclonal Antibody Production 20.2 Detecting Antigen-Antibody Complexes 20.3 Agglutination Assays 20.4 EIAs and ELISAs 20.5 Fluorescent Antibody Techniques Introduction Many laboratory tests are designed to confirm a presumptive diagnosis by detecting antibodies specific to a suspected pathogen. Unfortunately, many such tests are time-consuming and expensive. That is now changing, however, with the development of new, miniaturized technologies that are fast and inexpensive. For example, researchers at Columbia University are developing a “lab-on-a-chip” technology that will test a single drop of blood for 15 different infectious diseases, including HIV and syphilis, in a matter of minutes.[1] The blood is pulled through tiny capillaries into reaction chambers where the patient’s antibodies mix with reagents. A chip reader that attaches to a cell phone analyzes the results and sends them to the patient’s healthcare provider. Currently the device is being field tested in Rwanda to check pregnant women for chronic diseases. Researchers estimate that the chip readers will sell for about $100 and individual chips for $1.[2] 1.
    [Show full text]
  • Immunocytochemistry of Cytology Specimens for Predictive Biomarkers in Lung Cancer
    905 Review Article on Selected Highlights of the 2019 Pulmonary Pathology Society Biennial Meeting Immunocytochemistry of cytology specimens for predictive biomarkers in lung cancer Sinchita Roy-Chowdhuri Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA Correspondence to: Sinchita Roy-Chowdhuri, MD, PhD. Department of Pathology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd. Unit 85, Houston, TX 77030, USA. Email: [email protected]. Abstract: With a growing number of predictive biomarkers that have emerged in non-small cell lung carcinoma (NSCLC), there has been a paradigm shift in the management of these patients. Of the various predictive biomarker testing methods, immunohistochemistry (IHC) is the most widely available, cost- effective, and commonly used methods. However, most predictive IHC assays are validated primarily on formalin-fixed paraffin-embedded (FFPE) histologic tissue samples and translating these assays to cytologic specimens requires additional and rigorous validation. This is part due to the lack of standardized processing protocols in cytology resulting in a variety of preanalytic variables that can impact the antigenicity of antibodies used for predictive biomarker testing. The review article discusses the various preanalytical and analytical factors that impact immunocytochemistry (ICC) in cytologic specimens and summarizes the current published literature on ALK, ROS1, PD-L1, and other predictive biomarker ICC in cytology. Keywords: Immunocytochemistry (ICC); lung cancer; cytology; biomarkers; non-small cell lung carcinoma (NSCLC) Submitted Dec 14, 2019. Accepted for publication Dec 25, 2019. doi: 10.21037/tlcr.2019.12.31 View this article at: http://dx.doi.org/10.21037/tlcr.2019.12.31 Predictive biomarkers are markers of therapeutic efficacy, embedded (FFPE) histologic tissue samples, a large fraction the results of which are essential for determining patient of NSCLC patients are diagnosed on cytology samples, care.
    [Show full text]
  • EUROPEAN PHARMACOPOEIA Free Access to Supportive Pharmacopoeial Texts in the Field of Vaccines for Human Use During the Coronavirus Disease (COVID-19) Pandemic
    EUROPEAN PHARMACOPOEIA Free access to supportive pharmacopoeial texts in the field of vaccines for human use during the coronavirus disease (COVID-19) pandemic Updated package - October 2020 Published in accordance with the Convention on the Elaboration of a European Pharmacopoeia (European Treaty Series No. 50) European Directorate Direction européenne for the Quality de la qualité of Medicines du médicament & HealthCare & soins de santé Council of Europe Strasbourg Free access to supportive pharmacopoeial texts in the field of vaccines for human use during the coronavirus disease (COVID-19) pandemic Updated package The EDQM is committed to supporting users during the coronavirus disease (COVID-19) pandemic – as well as contributing to the wider global effort to combat the virus – by openly sharing knowledge and providing access to relevant guidance/standards. To support organisations involved in the development, manufacture or testing of COVID-19 vaccines worldwide, many of which are universities and small and medium-sized enterprises, the EDQM is offeringte mporary free access to texts of the European Pharmacopoeia (Ph. Eur.) in the field of vaccines. This package includes quality standards for vaccines which developers can take into account to help build appropriate analytical control strategies for their COVID-19 candidate vaccines and ensure the quality and safety of the final product. Application of such quality requirements may ultimately help to facilitate regulatory acceptance of a subsequent marketing authorisation application. For ease of reading, a summary table listing the pharmacopoeial texts, with information regarding the vaccine types or vaccine platforms concerned (e.g. live attenuated viral vaccine, recombinant viral-vectored vaccines) is provided.
    [Show full text]
  • Immunohistochemical (IHC) Marker Template for Integral Markers in Clinical Trials
    ! "#$%&'()*+,%-.',')'/!01!!!!!!!!!!!!!! -.',')'/!$(2*3,456,'.1!!!!!!!!!!!!!!!!!!!!!!!!!!! Immunohistochemical (IHC) Marker Template For Integral Markers in Clinical Trials This is a template to describe the analytical and clinical performance of an assay that is essential for performance of a trial. It will be used to assess whether assays are ready for use in a trial by Disease Steering Committees and CTEP. The FDA may also use it to evaluate integral assays and diagnostics for their pre-IDE evaluation. Not all parameters may be known a priori. Please enter as much information as you can and N/A for not available or applicable where appropriate. This template requires detailed information that may be known only by laboratorians, scientists who work in clinical laboratories, and should be collaborating closely with clinical trialists. Please be sure to collect the appropriate responses before filling out this form. The template has the following sections with information needed from trialists and laboratorians: 1. Assay, Patient and Specimen Information –Trialists and Laboratorians 2. Primary Antibody Characteristics – Laboratorians 3. Design of Immunohistochemical Assay - Laboratorians 4. Assay Performance – Laboratorians 5. Laboratory Information – Trialists and Laboratorians ! 7! ! "#$%&'()*+,%-.',')'/!01!!!!!!!!!!!!!! -.',')'/!$(2*3,456,'.1!!!!!!!!!!!!!!!!!!!!!!!!!!! Section 1. Assay, Patient and Specimen Information A. Name of marker (Please use HUGO gene or protein name for molecular marker or the Atlas for Genetics in Hematology and Oncology for cytogenetic or FISH markers) HUGO Site: http://www.genenames.org/ Atlas Site: http://atlasgeneticsoncology.org/index.html B. How will assay and its marker be used in clinical trial? Integral Marker Integrated Marker Research Marker • Integral markers are required for the trial to proceed (e.g., patient eligibility, assignment to treatment, stratification, risk classifier or medical decision-making - often requires performance in a CLIA laboratory).
    [Show full text]
  • Neural Cell Markers Neural Cell Markers
    Neural Cell Markers Neural Cell Markers Neurons and glia in neural tissue or cultures are commonly visualized and identified by immunodetection of cell-specific antigenic markers, including transcription factors, enzymes, cytoskeletal proteins, cell surface proteins, and secreted factors. The Bio-Techne brands R&D Systems and Novus Biologicals together offer an unparalleled selection of antibodies directed against these intracellular and cell surface proteins that can be used for the identification and characterization of different neural cell types. R&D Systems also offers a variety of different immunnoassays, including the gold standard Quantikine® ELISA Kits, for detecting secreted molecules. The Bio-Techne brand Tocris offers a novel and exclusive collection of tools, including bioactive small molecules, caged compounds, and fluorescent probes, for the functional identification of neural cells. Table of Contents Neuronal Markers . 1–8 General Markers. .1 Dendritic Markers. 2 Axonal Markers. 2 Presynaptic Markers. 3 Active Zone Markers. 4 Postsynaptic Markers. .4 Growth Cone Markers. .5 Cholinergic Neuron Markers. 6 Dopaminergic Neuron Markers. .6 GABAergic Neuron Markers. 7 Glutamatergic Neuron Markers. .7 Glycinergic Neuron Markers. 8 Serotonergic Neuron Markers. 8 Microglia Markers. 9–12 Steady-State Microglia Markers. .9 M1 Microglia Markers. .10–11 M2 Microglia Markers. 12 Astrocyte Markers. 13 General Markers. 13 Oligodendrocyte Markers. 14–15 Oligodendrocyte Precursor Cell Markers. .14 Immature Oligodendrocyte Markers . 14 Mature, Non-Myelinating Oligodendrocyte Markers. 15 Mature, Myelinating Oligodendrocyte Markers. 15 Additional Tools for Visualizing and Identifying Neural Cells. 16 Conjugated Primary Antibodies. 16 R&D Systems® VisUCyte™ HRP Polymer. 16 R&D Systems® Multiplex Assays . .16 Tocris® Products to Investigate Neural Function.
    [Show full text]
  • IHC/ICC Protocol Guide
    RnDSy-lu-2945 IHC/ICC Protocol Guide This guide provides an introduction to the techniques, protocols, and troubleshooting methods that lead to a successful immunohistochemistry (IHC) or immunocytochemistry (ICC) experiment. Contributors include scientists from our IHC/ICC core facility who have experience qualifying thousands of antibodies for these applications and our expert technical service team who have guided researchers through a range of IHC/ICC-related issues. To view these protocols online, please visit rndsystems.com/IHCProtocol. To learn more about R&D Systems brand antibodies, please visit rndsystems.com/Antibodies. This page also allows you to search for primary and secondary antibodies for your research. Use the drop-down menu to filter by species, application, and conjugate. Cover images generated using R&D Systems brand antibodies. To view the continuously updated IHC/ICC image gallery, please visit rndsystems.com/IHCimages. Table of Contents Introduction to the IHC/ICC Method. .1–11 IHC/ICC Protocols. 13–22 Designing a Successful IHC/ICC Experiment. 3 Making a 4% Formaldehyde Solution in PBS .....................14 Sample Preparation. 4 Preparation of Gelatin-Coated Slides for Histological Tissue Sections .............................. 15 Paraffin-Embedded Tissue Chromogenic Staining of Tissue Sections Frozen Tissue Using HRP-DAB Detection ................................16–17 Cell Samples Slide Preparation Fixation. 5 Chromogenic Staining Using HRP-DAB Detection Formaldehyde IHC Staining of Tissue Sections for Fluorescence Microscopy ...... 18 Alcohols Antigen Retrieval Using Heat-Induced Epitope Retrieval (HIER) ..... 19 Acetone Preparation of Coverslips and Cell Fixation for ICC ............... 20 Fixation of Tissues Coverslip Preparation Using Gelatin Fixation of Cultured Cells Preparation and Fixation of Cells on Coverslips Preventing Non-Specific Staining .
    [Show full text]
  • Immunocytochemistry (ICC) Handbook
    Novus-lu-2945 Immunocytochemistry (ICC) Handbook Learn more | novusbio.com Learn more | novusbio.com Excitation/ Emission Laser Conjugate Notes Emission Color (Excitation Source) DyLightTM 405 400/420 Violet Violet (405 nm) Bright and photostable Alexa Fluor® 405 401/421 Violet Violet (405 nm) Best when used with more abundant targets Can be used with DyLightTM 488, 594 and 647 DyLightTM 350 353/432 Violet-Blue Ultraviolet (355 nm) in multiplexing Often used with Alexa Fluor® 488, 594 and 647 in Alexa Fluor® 350 346/442 Violet-Blue Ultraviolet (355 nm) multiplexing, best for high-abundance targets Brighter, photostable replacement for FITC; DyLightTM 488 493/518 Green Blue (488) not suitable for use with GFP Photostable over a broad pH range; Alexa Fluor® 488 495/519 Green Blue (488) replaces FITC Small organic fluorophore; cannot be used with FITC 495/519 Green Blue (488) DyLightTM 488, Alexa Fluor® 488 or GFP Violet Superior alternative to Pacific Orange; good choice DyLightTM 405LS 397/572 Yellow (405 nm) for multicolor applications on the violet laser Photostable over a broad pH range; Alexa Fluor® 546 556/573 Yellow Yellow-Green (561 nm) brighter than Cy3 DyLightTM 550 562/576 Yellow Yellow-Green (561 nm) Subject to photobleaching; can be excited by the PE 565/578 Yellow Yellow-Green (561 nm) 488, 532, and 561nm lasers on flow cytometers Very bright fluorescence; use a tunable dye laser Texas Red® 595/613 Orange Yellow-Green (561 nm) to avoid leaking when multiplexed with PE Alexa Fluor® 594 590/617 Orange Yellow-Green (561 nm) Better photostability than Texas Red DyLightTM 650 654/673 Red Red (633 nm) Bright fluorescent protein; do not use with APC 650/660 Red Red (633 nm) DyLightTM 650 due to overlapping emission spec- tra Extremely photostable, good Alexa Fluor® 647 650/665 Red Red (633 nm) replacement for Cy5 or APC Some fluorescence quenching when Cy5TM 647/665 Red Red (633 nm) conjugated DyLightTM is a registered trademark of Thermo Fisher Scientific Inc.
    [Show full text]