Tree Scale: 1 1.00 0.64

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Geopsychrobacteraceae g Geopsychrobacter s Geopsychrobacter electrodiphilus WGS ID ARWE01 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Geopsychrobacteraceae g Seleniibacterium s Seleniibacterium seleniigenes WGS ID JOMG01 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Geoalkalibacteraceae g GeoalkalibacterA s GeoalkalibacterA subterraneus RS GCF 000827125 1 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Geopsychrobacteraceae g Malonomonas s Malonomonas rubra WGS ID FQZT01 1 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Trichloromonadaceae g Deferrimonas s Deferrimonas soudanensis RS GCF 001278055 1 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Geopsychrobacteraceae g SLDV01 s SLDV01 sp007125165 WGS ID SKQT01 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Geopsychrobacteraceae g BM522 s BM522 sp002869665 WGS ID PKUG01 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Geopsychrobacteraceae g Desulfuromusa s Desulfuromusa kysingii WGS ID FNQN01 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Trichloromonadaceae g Thrichloromonas s Thrichloromonas acetexigens WGS ID FOJJ01 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Trichloromonadaceae g SZUA-540 s SZUA-540 sp003250235 WGS ID QKGY01 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f SZUA-401 g SZUA-401 s SZUA-401 sp002869605 WGS ID PKUI01 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g PB-SRB1 s PB-SRB1 sp003500125 WGS ID AVFQ01 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g Desulfofustis s Desulfofustis glycolicus WGS ID FQXS01 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f BM103 g BM103 s BM103 sp002869725 WGS ID PKTY01 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g GCA-2747805 s GCA-2747805 sp002747805 WGS ID PDQP01 1 BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g MADRE3 s MADRE3 sp004796425 WGS ID QZLB01 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g UBA8130 s UBA8130 sp003509305 WGS ID DOMU01 1 BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g Desulforhopalus s Desulforhopalus sp005116645 WGS ID SWCM01 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g UBA2775 s UBA2775 sp002352445 WGS ID DEIL01 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g Desulfotalea s Desulfotalea psychrophila RS GCF 000025945 1 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f UBA5852 g UBA5852 s UBA5852 sp002432195 WGS ID DJGG01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f SG8-13 g SG8-13 s SG8-13 sp001303025 WGS ID LJNK01 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g PB-SRB1 s PB-SRB1 sp003500125 WGS IDAVFQ01 2

BD d Bacteria p Desulfobacterota c BSN033 o BSN033 f UBA1163 g RBG-16-49-23 s RBG-16-49-23 sp001798855 WGS ID MGQQ01 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g Tol-SR s Tol-SR sp000769715 WGS ID JROS01 1 BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g Desulfocapsa s Desulfocapsa sulfexigens RS GCF 000341395 1 1

BD d Bacteria p Desulfobacterota B c Binatia o UBA9968 f UBA9968 g DP-36 s DP-36 sp005878685 WGS ID VBLN01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfobacteraceae g NBML01 s NBML01 sp002085465 WGS ID NBML01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfobacteraceae g Desulfospira s Desulfospira joergensenii WGS ID ATUG01 1

BD Desulfopila inferna DSM19738 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g UBA2270 s UBA2270 sp002347745 WGS ID DDXC01 1 BD Desulforhopalus vacuolatus DSM9700 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfobulbaceae g Desulfobulbus s Desulfobulbus elongatus WGS ID JHZB01 1

BD Desulfotalea psychrophila 1 BD Desulfoprunum benzoelyticum DSM28570 1

BD d Bacteria p Desulfobacterota Desulfuromonadia c o Geobacterales f Geobacteraceae g Citrifermentans s Citrifermentans pelophilum WGS ID BDQG01 2

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Geobacteraceae g Citrifermentans s Citrifermentans sp000023645 RS GCF 000023645 1 2 BD Desulfocapsa sulfexigens DSM10523 1 BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfobulbaceae g Desulfobulbus A s Desulfobulbus A japonicus WGS ID AUCV01 1 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Geobacteraceae g Geotalea s Geotalea daltonii RS GCF 000022265 1 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfobulbaceae g Desulfobulbus B s Desulfobulbus B propionicus A WGS ID PDTC01 1

BD Desulfobulbus marinus DSM2058 2

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Trichloromonadaceae g ATBO01 s ATBO01 sp000472285 WGS ID ATBO01 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Syntrophotaleaceae g Syntrophotalea s Syntrophotalea sp002435955 WGS ID DIWZ01 2

BD d Bacteria p Desulfobacterota c MBNT15 o MBNT15 f MBNT15 g FEB-35 s FEB-35 sp003105225 WGS ID PQAK01 1

BD Desulfocapsa thiozymogenes 1 BD d Bacteria p Desulfobacterota c MBNT15 o MBNT15 f MBNT15 g RBG-16-64-85 s RBG-16-64-85 sp001798885 WGS ID MGQW01 1

BD d Bacteria p Desulfobacterota c MBNT15 o MBNT15 f MBNT15 g GWC2-65-14 s GWC2-65-14 sp001797615 WGS ID MGPV01 1

BD Desulfobulbus propionicus DSM2032 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfobulbaceae g QNYF01 s QNYF01 sp003978695 WGS ID QNYD01 1 BD Desulfobulbus rhabdoformis DSM8777 1

BD Desulfobulbus alkaliphilus DSM24258 1 BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f Smithellaceae g UBA4810 s UBA4810 sp001797845 WGS ID MGQN01 1 BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f UBA2251 g UBA2251 s UBA2251 sp002347815 WGS ID DDXV01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f QNYZ01 g QNYZ01 s QNYZ01 sp003973265 WGS ID QNYZ01 1

BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f Smithellaceae g UBA8904 s UBA8904 sp002840435 WGS ID PHAT01 1

BD d Bacteria p Desulfobacterota c Dissulfuribacteria o Dissulfuribacterales f QOAL01 g QOAL01 s QOAL01 sp003978135BD WGS Desulfobulbus ID QOAL01 marinus 1 DSM2058 1 BD d Bacteria p Desulfobacterota c Desulfarculia o Desulfarculales f Desulfarculaceae g Desulfocarbo s Desulfocarbo indianensis WGS ID LBMP01 2

BD d Bacteria p Desulfobacterota c Syntrophobacteria o Syntrophobacterales f Syntrophobacteraceae g SbD1 s SbD1 sp900290365 WGS ID OMOE01 3 1.00 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfosarcinaceae g Desulfosarcina s Desulfosarcina cetonica WGS ID BBCC01 2

BD d BacteriaBD Desulfobacter p Desulfobacterota hydrogenophilus c DesulfobacteriaBD d DSM3380 Bacteria o Desulfobacterales 3 p Desulfobacterota f Desulfobacteraceae c Desulfobacteria g Desulfobacterium o Desulfobacterales B s Desulfobacterium f BUBA2174 autotrophicum g UBA2174 RS GCF 000020365 s UBA2174 1 2 sp002327445 WGS ID DCWA01 2 BD d Bacteria p Desulfobacterota c Syntrophobacteria o Syntrophobacterales f Syntrophobacteraceae g Desulforhabdus s Desulforhabdus sp007244385 WGS ID SCVX01 3 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfosarcinaceae g Desulfatitalea s Desulfatitalea tepidiphila WGS ID BCAG01 2 BD d BacteriaBD d Bacteria p Desulfobacterota p Desulfobacterota c Desulfobacteria c Syntrophobacteria o Desulfobacterales o BM002 f UBA2156 f BM002 g GCA-002779465g BM002 s BM002 s GCA-002779465 sp002899795 WGS sp002771315 ID PPDG01 WGS 1 ID PCSF01 1 BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfurivibrionaceae g DB1 s DB1 sp001914235 WGS ID MQUF01 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfurivibrionaceae g SURF-16 s SURF-16 sp003605035 WGS ID QZKJ01 1 BD Desulfobulbus oralis 1

0.85

BD d Bacteria p Desulfobacterota c Syntrophorhabdia o Syntrophorhabdales f WCHB1-27 g BOG-1155 s BOG-1155 sp002067405 WGS ID MVRP01 3 BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfurivibrionaceae g BM506 s BM506 sp002868945 WGS ID PKTX01 1 BD Desulfococcus multivorans DSM2059 2 BD d Bacteria p DesulfobacterotaBD cd DesulfobacteriaBacteria p Desulfobacterota o Desulfatiglandales c Desulfobacteria f HGW-15 g HGW-15 o Desulfobacterales s HGW-15 sp002840535 f Desulfococcaceae WGS ID PHBH01 g 1Desulfococcus s Desulfococcus multivorans WGS ID ATHJ01 2 BD d Bacteria p DesulfobacterotaBD d Bacteria c Desulfobacteria p Desulfobacterota o Desulfobacterales c Desulfobacteria f Desulfococcaceae o Desulfobacterales g Desulfonema f Desulfosarcinaceae s Desulfonema g ishimotonii UBA2230 WGS s UBA2230 ID BEXT01 sp002348005 2 WGS ID DDYQ01 1 0.89 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f SURF-15 g SURF-15 s SURF-15 sp003599475 WGS ID QZKK01 2 BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfurivibrionaceae g UBA2262 s UBA2262 sp002347095 WGS ID RCMW01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f SKZT01 g SKZT01 s SKZT01 sp007127235 WGS ID PXDV01 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g UBA6079 s UBA6079 sp002428705 WGS ID DIXN01 2 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfatirhabdiaceae g RAAP-1 s RAAP-1 sp001443525 WGS ID LKPX01 2 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Desulfuromonadaceae g Pseudodesulfuromonas s Pseudodesulfuromonas thiophila WGS ID FNAQ01 1 BD d Bacteria p Desulfobacterota c DesulfobacteriaBD o Desulfobacterales d Bacteria p Desulfobacterota f Desulfatirhabdiaceae c Desulfovibrionia g UBA4064 s oUBA4064 Desulfovibrionales sp002382065 f Desulfovibrionaceae WGS ID DFZE01 3 g Pseudodesulfovibrio s Pseudodesulfovibrio hydrargyri WGS ID LKAQ01 2 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Alteridesulfovibrio s Alteridesulfovibrio inopinatus WGS ID AUBP01 2

BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Solidesulfovibrio s Solidesulfovibrio sp000177215 WGS ID ADFE02 2 BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f UBA2192 g UBA2192 s UBA2192 sp002327315 WGS ID DCVI01 1 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Desulfuromonadaceae g Desulfuromonas s Desulfuromonas acetoxidans WGS ID AAEW02 1 BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f UBA2207 g UBA4054 s UBA4054 sp002383495 WGS ID DIKY01 1 0.99 BD d Bacteria p Desulfobacterota c Syntrophobacteria o Syntrophobacterales f Syntrophobacteraceae g Delta-01 s Delta-01 sp001603845 WGS ID LUZZ01 2 BD d Bacteria p Desulfobacterota c Syntrophobacteria o Syntrophobacterales f Syntrophobacteraceae g Syntrophobacter s Syntrophobacter fumaroxidans RS GCF 000014965 1 2 BD d BacteriaBD d pBacteria Desulfobacterota p Desulfobacterota c Syntrophobacteria c Syntrophia o Syntrophobacteraleso Syntrophales f UBA4778 f Syntrophobacteraceae g UBA4778 s UBA4778 g Syntrophobacter sp002403175 s Syntrophobacter WGS ID DHHG01 fumaroxidans 1 RS GCF 000014965 1 3 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfoplanaceae g Desulfoplanes s Desulfoplanes formicivorans WGS ID BDFE01 1 BD d Bacteria BDp Desulfobacterota d Bacteria p Desulfobacterota c Syntrophia o Syntrophalesc Syntrophobacteria f UBA2185 o Syntrophobacterales g Fen-1135 s Fen-1135 f Syntrophobacteraceae sp003153775 WGS ID g UBA696PMBI01 1s UBA696 sp002298165 WGS ID DBMI01 2

1.00 BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f UBA6109 g UBA6109 s UBA6109 sp002428445 WGS ID DIWJ01 1 BD Desulfopila inferna DSM19738 2 BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfocapsaceae g Desulforhopalus s Desulforhopalus sp005116645 WGS ID SWCM01 2

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfatiglandales f NaphS2 g NaphS2 s NaphS2 sp000179315 WGS ID ADZZ01 1

0.99

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfurivibrionaceae g UBA5123 s UBA5123 sp002414225 WGS ID DHYV01 1

BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfurivibrionaceae g UBA5628 s UBA5628 sp002421565 WGS ID DIKC01 1

0.31 BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfurivibrionaceae g CG2-30-60-27 s CG2-30-60-27 sp001873115 WGS ID MNYK01 1 0.20 0.46 0.86 0.27 Tree scale: 1 1.00 0.64

0.67 0.77 1.00 1.00 1.00 BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f BM004 g YD12-FULL-57-12 s YD12-FULL-57-12 sp001799275 WGS ID MGTE01 1 1.00 0.99 1.001.00 1.00 1.00 0.98 0.78 1.000.700.44 0.890.86 0.81 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Trichloromonadaceae g DDH964 s DDH964 sp001611275 RS GCF 001611275 1 1 0.560.53 1.00 0.25 0.60 0.790.75 0.62 0.830.83 0.81 0.87 0.52 0.59 0.76 0.87 0.91 0.93 0.99 0.71 0.65 0.68 1.00 0.97 0.63 0.79 1.00 0.96 0.83 0.97 1.00 0.86 0.93 0.95 BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f BM004 g BM004 s BM004 sp002868955 WGS ID PKTW01 1 0.53 0.76 0.86 0.99 0.840.92 1.00 0.69 0.96 0.770.70 0.530.740.590.760.770.740.800.770.880.95 0.99 0.90

1.00

1.00 0.99 0.99

1.00 1.00

1.00

BD Desulfofundulus thermobenzoicus subsp thermosyntrophicus DSM14055 1 1.00 0.98 BD Desulfotomaculum nigrificans 1

1.00 0.98

0.85 0.99

0.81

0.97 BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f Fen-1087 g Fen-1087 s Fen-1087 sp003161855 WGS ID PMAY01 1 BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f UBA8958 g UBA8958 s UBA8958 sp003451635 WGS ID DMAT01 1 BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f PHBD01 g PHBD01 s PHBD01 sp002841865 WGS ID PHBD01 1 1.00 BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f Syntrophaceae g Syntrophus s Syntrophus gentianae WGS ID FOBS01 1

1.00 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Geobacteraceae g Citrifermentans s Citrifermentans pelophilum WGS ID BDQG01 1 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Geobacteraceae g Citrifermentans s Citrifermentans sp000023645 RS GCF 000023645 1 1 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Trichloromonadaceae g UBA2197 s UBA2197 sp002841765 WGS ID PHAV01 1 1.00 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Syntrophotaleaceae g SFB93 s SFB93 sp001887775 RS GCF 001887775 1 1 1.00 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Syntrophotaleaceae g Syntrophotalea s Syntrophotalea carbinolica RS GCF 000012885 1 1 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Syntrophotaleaceae g Syntrophotalea s Syntrophotalea sp002435955 WGS ID DIWZ01 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Geobacteraceae g RPRC01 s RPRC01 sp003857395 WGS ID RPRC01 1

1.00 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Geobacteraceae g UBA4078 s UBA4078 sp003314535 WGS ID POSG01 1 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Geobacteraceae g QAXR01 s QAXR01 sp003053505 WGS ID QAXR01 1 0.56 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Desulfuromonadales f Trichloromonadaceae g BM515 s BM515 sp002869635 WGS ID PKUF01 1 0.69 1.00 1.00 0.990.860.570.75 0.770.88 0.71 BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f UBA2207 g UBA2207 s UBA2207 sp002418885 WGS ID DCUT01 1 1.00 0.92 0.85 0.800.690.740.550.97 0.960.71 BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f UBA6807 g UBA6807 s UBA6807 sp002441265 WGS ID DKFB01 1 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Geobacteraceae g H2GEO s H2GEO sp003574885 WGS ID PJFC01 1

0.99 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Pseudopelobacteraceae g Pseudopelobacter s Pseudopelobacter sp003574895 WGS ID PJFB01 1

BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Geobacteraceae g OR-1 s OR-1 sp000813145 WGS ID BAZF01 1 0.76 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Pseudopelobacteraceae g Trichlorobacter s Trichlorobacter thiogenes WGS ID FUWR01 1 0.900.770.68 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Geobacteraceae g Geobacter s Geobacter metallireducens WGS ID AGJM01 1 0.710.58 0.97 0.79 BD d Bacteria p Desulfobacterota c Desulfuromonadia o Geobacterales f Geobacteraceae g Geobacter s Geobacter sulfurreducens RS GCF 003574175 1 1 0.65 0.90 0.840.73 0.69 1.001.00 0.91 0.830.980.94 0.77 0.61 0.99 0.92 0.98 0.870.44 0.70 0.79 0.91 1.00 0.45 0.951.00 0.910.69 0.24 1.00

0.92

0.94 1.00 1.00 0.88 0.74 0.94 0.93 0.61 0.99 0.53 0.72 0.82 1.00 0.97 0.83 1.00 0.99 0.61 1.00 0.66 1.00 0.90 1.00 0.85 1.00 0.78 0.49 1.00 0.750.42 0.72 0.87 0.82 0.57 0.97 0.850.840.760.45 0.68 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfonatronaceae g Desulfonatronum s Desulfonatronum thiodismutans WGS ID JPIK01 1 0.420.860.20 0.69 0.93 0.650.41 1.00 0.920.900.990.82 0.99 0.96 1.00 1.00 0.840.85 0.75 0.95 BD Desulfobacter curvatus DSM3379 1 0.571.00 1.00 0.62 0.16 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfonatronovibrionaceae g Desulfonatronospira s Desulfonatronospira thiodismutans WGS ID ACJN02 1 0.92 1.00 0.36 0.92 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfonatronovibrionaceae g Desulfonatronovibrio s Desulfonatronovibrio hydrogenovorans WGS ID JMKT01 1 0.81 0.89 1.00 BD Desulfobacter hydrogenophilus DSM3380 1 1.00 1.00 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfobacteraceae g Desulfobacter s Desulfobacter postgatei WGS ID AGJR02 1 1.00 0.94 BD Desulfurivibrio alkaliphilus AHT2 1

1.00 BD d Bacteria p Desulfobacterota c Desulfobulbia o Desulfobulbales f Desulfurivibrionaceae g Desulfurivibrio s Desulfurivibrio alkaliphilus RS GCF 000092205 1 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfonauticaceae g Desulfonauticus s Desulfonauticus submarinus WGS ID FNIN01 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfohalobiaceae A g Desulfothermus s Desulfothermus okinawensis WGS ID BBBM01 2 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfohalobiaceae g Desulfohalobium s Desulfohalobium retbaense RS GCF 000024325 1 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfobacteraceae g Desulfotignum s Desulfotignum phosphitoxidans WGS ID APJX01 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfomicrobiaceae g Desulfomicrobium s Desulfomicrobium sp002841885 WGS ID PHBE01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfobacteraceae g Desulfobacula s Desulfobacula toluolica WGS ID FNLL01 1 BD d BacteriaBD d Bacteria p Desulfobacterota p Desulfobacterota c Desulfovibrionia c Desulfovibrionia o Desulfovibrionales o Desulfovibrionales f Desulfomicrobiaceae f Desulfovibrionaceae g UBA12183 g Maridesulfovibrio s UBA12183 sp003530935 s Maridesulfovibrio WGS bastiniiID DPEO01 WGS 1 ID AUCX01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfobacteraceae g Desulfobacula s Desulfobacula toluolica RS GCF 000307105 1 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfobacteraceae g MADRE2 s MADRE2 sp004796375 WGS ID QZLA01 1 BD d BacteriaBD d Bacteria p Desulfobacterota p Desulfobacterota c Desulfovibrionia c Desulfovibrionia o Desulfovibrionales o Desulfovibrionales f Desulfovibrionaceae f Desulfovibrionaceae g Desulfovibrio g MADRE1 Q s Desulfovibrio s MADRE1 sp004796465Q ferrophilus RS WGS GCF ID 003966735QZKZ01 1 1 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Desulfocurvus s Desulfocurvus vexinensis WGS ID JAEX01 1

BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Paradesulfovibrio s Paradesulfovibrio bizertensis WGS ID FUYA01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfobacteraceae g Desulfobacterium A s Desulfobacterium A vacuolatum WGS ID FWXY01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfobacteraceae g Desulfamplus s Desulfamplus magnetovallimortis WGS ID FWEV01 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Desulfohalovibrio s Desulfohalovibrio alkalitolerans WGS ID ATHI01 1 BD d BacteriaBD d Bacteria p Desulfobacterota p Desulfobacterota c Desulfovibrionia c Desulfovibrionia o Desulfovibrionales o Desulfovibrionales f Desulfovibrionaceae f Desulfovibrionaceae g Desulfocurvibacter g UBA6079 s sUBA6079 Desulfocurvibacter sp002428705 africanus WGS RS ID DIXN01GCF 000212675 1 2 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f B30-G6 g B30-G6 s B30-G6 sp003647375 WGS ID QMMS01 2 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Desulfocurvibacter s Desulfocurvibacter africanus WGS ID AOSV01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfobacteraceae g Desulfobacterium B s Desulfobacterium B autotrophicum RS GCF 000020365 1 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Paucidesulfovibrio s Paucidesulfovibrio gracilis WGS ID FUYC01 1

BD Desulfococcus multivorans DSM2059 1 BD Desulfovibrio brasiliensis JCM12178 1 BD Desulfovibrio oxyclinae DSM11498 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f SURF-7 g SURF-7 s SURF-7 sp003599865 WGS ID QZKS01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfosarcinaceae g Desulfosarcina s Desulfosarcina cetonica WGS ID BBCC01 1 BD Desulfovibrio aminophilus DSM12254 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Aminidesulfovibrio s Aminidesulfovibrio aminophilus WGS ID AUMA01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f SURF-3 g SURF-33 s SURF-33 sp003597945 WGS ID QZJS01 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Pseudodesulfovibrio s Pseudodesulfovibrio piezophilus RS GCF 000341895 1 1 BD Desulfovibrio sulfodismutans DSM3696 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Alteridesulfovibrio s Alteridesulfovibrio inopinatus WGS ID AUBP01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f SURF-3 g SURF-3 s SURF-3 sp003599885 WGS ID QZKW01 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Pseudodesulfovibrio s Pseudodesulfovibrio hydrargyri WGS ID LKAQ01 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Humidesulfovibrio s Humidesulfovibrio mexicanus WGS ID FZOC01 1 BD Desulfovibrio mexicanus DSM13116 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Fundidesulfovibrio s Fundidesulfovibrio putealis WGS ID AUBQ01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f UBA5616 g UBA5616 s UBA5616 sp002423615 WGS ID DIKO01 1

BD d BacteriaBD d Bacteria p Desulfobacterota p Desulfobacterota c Desulfovibrionia c Desulfovibrionia o Desulfovibrionales o Desulfovibrionales f Desulfovibrionaceae f Desulfovibrionaceae g Megalodesulfovibrio g Solidesulfovibrio s Megalodesulfovibrio s Solidesulfovibrio gigas sp000177215WGS ID AUBO01 WGS 1 ID ADFE02 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f RPRI01 g RPRI01 s RPRI01 sp003818505 WGS ID RPRI01 1

BD d Bacteria p Desulfobacterota c Dissulfuribacteria o Dissulfuribacterales f Dissulfuribacteraceae g Dissulfuribacter s Dissulfuribacter thermophilus WGS ID MAGO01 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Nitratidesulfovibrio s Nitratidesulfovibrio termitidis WGS ID AZAO01 1

BD d Bacteria p Desulfobacterota c Dissulfuribacteria o Dissulfuribacterales f UBA5754 g UBA5754 s UBA5754 sp002419025 WGS ID DIFG01 1 BD d Bacteria p DesulfobacterotaBD d Bacteria p c Desulfobacterota Desulfovibrionia o Desulfovibrionalesc Desulfovibrionia f oDesulfovibrionaceae Desulfovibrionales g fLawsonia Desulfovibrionaceae s Lawsonia intracellularis g Desulfovibrio WGS sID Desulfovibrio MTPJ01 1 sp002237645 WGS ID MJUZ01 1 BD d BacteriaBD pd DesulfobacterotaBacteria p Desulfobacterota c Desulfovibrionia c Desulfovibrionia o Desulfovibrionales o Desulfovibrionales f Desulfovibrionaceae f Desulfovibrionaceae g Mailhella s Mailhella g Lawsonia sp003150275 s Lawsonia WGS intracellularis ID UQYY01 1RS GCF 000055945 1 1

BD d Bacteria p Desulfobacterota c Desulfarculia o Desulfarculales f Desulfarculaceae g Desulfocarbo s Desulfocarbo indianensis WGS ID LBMP01 1 BD Desulfovibrio vulgaris 1

BD d Bacteria p Desulfobacterota c DesulfovibrioniaBD d Bacteria o Desulfovibrionales p Desulfobacterota f Desulfovibrionaceae c Desulfovibrionia g Frigididesulfovibrio o Desulfovibrionales s Frigididesulfovibrio f Desulfovibrionaceae cuneatus WGS ID g AUCY01 Halodesulfovibrio 1 s Halodesulfovibrio aestuarii WGS ID ARQF01 1 BD d Bacteria p Desulfobacterota c Desulfarculia o Desulfarculales f Desulfarculaceae g Dethiosulfatarculus s Dethiosulfatarculus sandiegensis WGSAZAC01 ID 1 BD d Bacteria p Desulfobacterota c Thermodesulfobacteria o Thermodesulfobacteriales f Thermodesulfatatoraceae g ThermodesulfatatorBD d Bacteria s Thermodesulfatator p Desulfobacterota atlanticus WGS ID c ATXH01 Desulfovibrionia 1 o Desulfovibrionales f Desulfovibrionaceae g Oleidesulfovibrio s Oleidesulfovibrio alaskensis RS GCF 000012665 1 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfococcaceae g Desulfococcus s Desulfococcus multivorans WGS ID ATHJ01 1 BD d Bacteria p Desulfobacterota c Desulfovibrionia o Desulfovibrionales f Desulfovibrionaceae g Oleidesulfovibrio s Oleidesulfovibrio alaskensis WGS ID AXWQ01 1

BD d Bacteria p Desulfobacterota c Thermodesulfobacteria o Thermodesulfobacteriales f Thermodesulfobacteriaceae g Thermosulfurimonas s Thermosulfurimonas dismutans WGS ID LWLG01 1

BD d Bacteria p Desulfobacterota c Thermodesulfobacteria o Thermodesulfobacteriales f Thermodesulfobacteriaceae g Caldimicrobium s Caldimicrobium sp002441555BD WGSd Bacteria ID DJWK01 1 p Desulfobacterota c Thermodesulfobacteria o Thermodesulfobacteriales f Thermodesulfobacteriaceae g QOAM01 s QOAM01 sp003978075 WGS ID QOAM01 1

BD d Bacteria p Desulfobacterota c Thermodesulfobacteria o Thermodesulfobacteriales f Thermodesulfobacteriaceae g Caldimicrobium s Caldimicrobium thiodismutans RS GCF 001548275 1 1

BD d Bacteria p Desulfobacterota c MBNT15 o MBNT15 f MBNT15 g UBA2219 s UBA2219 sp002327765 WGS ID DCUH01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfococcaceae g Desulfonema s Desulfonema ishimotonii WGS ID BEXT01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfatiglandales f NaphS2 g UBA5623 s UBA5623 sp900258555 WGS ID OJIN01 1 BD d Bacteria p Desulfobacterota c Thermodesulfobacteria o Thermodesulfobacteriales f Thermodesulfobacteriaceae g Thermodesulfobacterium s Thermodesulfobacterium commune RS GCF 000734015 1 1 BD d Bacteria p Desulfobacterota c Thermodesulfobacteria o Thermodesulfobacteriales f Thermodesulfobacteriaceae g Thermodesulfobacterium s Thermodesulfobacterium commune WGS ID JQLF01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfatiglandales f CG2-30-51-40 g CG2-30-51-40 s CG2-30-51-40 sp001874005 WGS ID MNYJ01 1 BD d Bacteria p Desulfobacterota c Syntrophia o Syntrophales f CG2-30-49-12 g CG2-30-49-12 s CG2-30-49-12 sp001873675 WGS ID PCQZ01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f UBA2174 g UBA2174 s UBA2174 sp002327445 WGS ID DCWA01 1

BD d Bacteria pBD Desulfobacterota d Bacteria p Desulfobacterota c Desulfobacteria c Desulfobacteria o Desulfobacterales o Desulfobacterales f YD0425bin51 f Desulfatirhabdiaceaeg YD0425bin51 s YD0425bin51 g RAAP-1 s sp002753225 RAAP-1 sp001443525 WGS ID PDZS01WGS ID LKPX011 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfatirhabdiaceae g UBA4064 s UBA4064 sp002382065 WGS ID DFZE01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f B30-G6 g B30-G6 s B30-G6 sp003647375 WGS ID QMMS01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f SURF-15 g SURF-15 s SURF-15 sp003599475 WGS ID QZKK01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f BuS5 g UBA11574 s UBA11574 sp002868985 WGS ID PKTV01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfosarcinaceae g Desulfatitalea s Desulfatitalea tepidiphila WGS ID BCAG01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f YD0425bin50 g YD0425bin50 s YD0425bin50 sp002753105 WGS ID PDZT01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Magnetomoraceae g Magnetomorum s Magnetomorum sp002753725 WGS ID PEAL01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f GCA-2748835 g GCA-2748835 s GCA-2748835 sp002748835 WGS ID PDTL01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfolunaceae g Desulfoluna s Desulfoluna spongiiphila WGS ID FMUX01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f UBA5852 g UBA5852 s UBA5852 sp002432195 WGS ID DJGG01 2 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulforegulaceae g Desulfobotulus s Desulfobotulus sp006175995 WGS ID VDMB01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulforegulaceae g Desulforegula s Desulforegula conservatrix WGS ID AUEY01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfatibacillaceae g Desulfatibacillum s Desulfatibacillum aliphaticivorans WGS ID AUCT01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfosudaceae g Desulfosudis s Desulfosudis oleovorans RS GCF 000018405 1 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f Desulfatibacillaceae g Desulfatibacillum s Desulfatibacillum aliphaticivorans RS GCF 000021905 1 1

BD d Bacteria p Desulfobacterota c Syntrophobacteria o B119-G9 f B119-G9 g B119-G9 s B119-G9 sp003646715 WGS ID QMLL01 1 BD d Bacteria p Desulfobacterota c Desulfobacteria o BDDesulfobacterales d Bacteria p Desulfobacterota f Desulfatirhabdiaceae c Syntrophorhabdia g Desulfatirhabdium o Syntrophorhabdales s Desulfatirhabdium f WCHB1-27 g butyrativorans BOG-1155 s BOG-1155 WGS ID AUCU01sp002067405 1 WGS ID MVRP01 1

BD d Bacteria p Desulfobacterota c Desulfobacteria o Desulfobacterales f UBA2156 g RBG-13-49-15 s RBG-13-49-15 sp001797695 WGS ID MGQD01 1

BD d Bacteria p Desulfobacterota c Syntrophorhabdia o Syntrophorhabdales f WCHB1-27 g WCHB1-27 s WCHB1-27 sp002328485 WGS ID DMVO01 1

BD d Bacteria p Desulfobacterota c Desulfobaccia o Desulfobaccales f 0-14-0-80-60-11 g SURF-52 s SURF-52 sp003599195 WGS ID QZIZ01 1

BD d Bacteria p Desulfobacterota c SyntrophobacteriaBD d Bacteria p Desulfobacterota o Syntrophobacterales c Syntrophobacteria f Syntrophobacteraceae o Syntrophobacterales g Delta-01 Syntrophobacteraceae f s Delta-01 sp001603845 g SbD1 s SbD1 WGS sp900290365 ID LUZZ01 WGS 1 ID OMOE01 1

BD d Bacteria p Desulfobacterota c Desulfarculia o Desulfarculales f Desulfarculaceae g JdFR-95 s JdFR-95 ID MTPE01 sp002011835 1 WGS

BD d Bacteria p Desulfobacterota c Desulfarculia o Desulfarculales f Desulfarculaceae g SURF-10 s SURF-10 sp003605055 WGS ID QZKP01 1

BD d Bacteria p Desulfobacterota c Syntrophobacteria o Syntrophobacterales f DSM-9756 g Desulfosoma s Desulfosoma caldarium WGS ID RJVA01 1 BD d Bacteria p Desulfobacterota c Syntrophobacteria o Syntrophobacterales f Syntrophobacteraceae g UBA696 s UBA696 sp002298165 WGS ID DBMI01 1

BD d Bacteria p Desulfobacterota c Desulfarculia o Desulfarculales f Desulfarculaceae g Desulfarculus s Desulfarculus baarsii RS GCF 000143965 1 1 BD d Bacteria p Desulfobacterota c Syntrophorhabdia o Syntrophorhabdales f Syntrophorhabdaceae g Syntrophorhabdus s Syntrophorhabdus aromaticivorans WGS ID AJUN01BD d Bacteria 1p Desulfobacterota c Syntrophobacteria o Syntrophobacterales f DSM-9756 g Desulfacinum s Desulfacinum hydrothermale WGS ID FWXF01 1

BD d Bacteria p Desulfobacterota c Desulfobaccia o Desulfobaccales f 0-14-0-80-60-11 g 0-14-0-80-60-11 s 0-14-0-80-60-11 sp002779455 WGS ID PEWM01 1

BD d Bacteria p Desulfobacterota c Desulfomonilia o Desulfomonilales f Desulfomonilaceae g Desulfomonile s Desulfomonile tiedjei RS GCF 000266945 1 2

BD d Bacteria p Desulfobacterota c Syntrophobacteria o Syntrophobacterales f Syntrophobacteraceae g Desulforhabdus s Desulforhabdus sp007244385 WGS ID SCVX01 1 BD d Bacteria p Desulfobacterota c Desulfomonilia o Desulfomonilales f Desulfomonilaceae g Desulfomonile s Desulfomonile tiedjei RS GCF 000266945 1 1 BD d Bacteria p Desulfobacterota c Syntrophobacteria o Syntrophobacterales f Syntrophobacteraceae g Syntrophobacter s Syntrophobacter fumaroxidans RS GCF 000014965 1 1

BD d Bacteria p Desulfobacterota c Syntrophobacteria o Syntrophobacterales f Thermodesulforhabdaceae g Thermodesulforhabdus s Thermodesulforhabdus norvegica WGS ID FOUU01 1