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Atrazine Chlorohydrolase from Pseudomonas Sp
JOURNAL OF BACTERIOLOGY, Aug. 1996, p. 4894–4900 Vol. 178, No. 16 0021-9193/96/$04.0010 Copyright q 1996, American Society for Microbiology Atrazine Chlorohydrolase from Pseudomonas sp. Strain ADP: Gene Sequence, Enzyme Purification, and Protein Characterization† 1,2 2,3,4 1,2,4 MERVYN L. DE SOUZA, MICHAEL J. SADOWSKY, AND LAWRENCE P. WACKETT * Department of Biochemistry and Biological Processes Technology Institute,1 Department of Soil, Water, and Climate,3 Department of Microbiology,4 and Center for Biodegradation Research and Informatics,2 University of Minnesota, St. Paul, Minnesota 55108 Received 26 April 1996/Accepted 30 May 1996 Pseudomonas sp. strain ADP metabolizes atrazine to carbon dioxide and ammonia via the intermediate hydroxyatrazine. The genetic potential to produce hydroxyatrazine was previously attributed to a 1.9-kb AvaI DNA fragment from strain ADP (M. L. de Souza, L. P. Wackett, K. L. Boundy-Mills, R. T. Mandelbaum, and M. J. Sadowsky, Appl. Environ. Microbiol. 61:3373–3378, 1995). In this study, sequence analysis of the 1.9-kb AvaI fragment indicated that a single open reading frame, atzA, encoded an activity transforming atrazine to hydroxyatrazine. The open reading frame for the chlorohydrolase was determined by sequencing to be 1,419 nucleotides and encodes a 473-amino-acid protein with a predicted subunit molecular weight of 52,421. The deduced amino acid sequence matched the first 10 amino acids determined by protein microsequencing. The protein AtzA was purified to homogeneity by ammonium sulfate precipitation and anion-exchange chroma- tography. The subunit and holoenzyme molecular weights were 60,000 and 245,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration chromatography, respectively. -
Biodegradation of Atrazine by Atrazine Chlorohydrolase: Characterization of Mutant Enzyme and Immobilization System for Water Purification
Biodegradation of Atrazine by Atrazine Chlorohydrolase: Characterization of Mutant Enzyme and Immobilization System for Water Purification. A THESIS SUBMITTED TO THE FACULTY OF THE GRADUATE SCHOOL OF THE UNIVERSITY OF MINNESOTA BY Amit Aggarwal IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE Lawrence P. Wackett & Ping Wang JANUARY 2012 © Amit Aggarwal 2011 Acknowledgement I want to thank my advisor, Dr. Larry Wackett, for the opportunity to work in his laboratory, his support and patience, and for allowing me to work independently and always being there to guide me. I would like to thank my mentor Dr. Jennifer Seffernick, for training me on different techniques in laboratory, and patiently helping me out throughout my association with the lab and teaching me good laboratory practices for research. Thank you so much for taking me under your wing. You are the best mentor I ever had. I would also like to thank Dr. Alptekin Aksan and Dr. Mike Sadowsky for guiding us throughout the immobilization of atrazine chlorohydrolase project. Dr. Alptekin Aksan guided us in development of a robust and efficient immobilization system and Dr. Mike Sadowsky provided us with valuable direction about making the immobilization system safe to be used in water purifications systems. I would like to thank my administrative adviser Dr. Ping Wang for always being supportive of my work and helping me out with planning my degree and ensuring that I meet all requirements in time. I am thankful to each of my committee members, Dr. Larry Wackett, Dr. Ping Wang and Dr. Jonathan Schilling for their time, for reading my thesis and for all the helpful suggestions. -
Atrazine Mineralization Potential and Catabolic Gene Detection In
ATRAZINE MINERALIZATION POTENTIAL AND CATABOLIC GENE DETECTION IN AGRICULTURAL AND WETLAND SITES DISSERTATION Presented in Partial Fulfillment of the Requirements of the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Kristen Lynn Anderson, B. S. * * * * * The Ohio State University 2003 Dissertation Committee: Approved by Dr. Olli H. Tuovinen, Advisor Dr. Michael J. Boehm _____________________________ Dr. Mark Morrison Advisor Dr. Samuel J. Traina Department of Microbiology ABSTRACT Atrazine (2-chloro-4-ethylamine-6-isopropylamino-1,3,5 triazine) is a commonly applied herbicide in corn fields. Although the fate of atrazine in agricultural systems has been well studied, the environmental fate of atrazine in wetland systems is less well characterized. The majority of research in this area has focused on aerobic mineralization of atrazine, although anaerobic conditions are commonly found in wetland sediments and bulk soils associated with agricultural fields. The hypothesis for this work was that atrazine would be actively mineralized in agricultural and wetland sites. It was further hypothesized that active mineralization in soils could be correlated with the presence of selected genes involved in atrazine metabolism. Soil, sediment, and water samples were obtained from three sites in Ohio. Atrazine mineralization was investigated under aerobic and anaerobic conditions in these samples using a biometer system in 14 which CO2 evolution was correlated with atrazine mineralization. All samples mineralized atrazine under aerobic conditions. Under anaerobic conditions, some external electron acceptor amendments inhibited mineralization, while others enhanced it. The effect on mineralization varied with the sample and season. Attempts were made to amplify some of the genes involved in atrazine mineralization. -
Proteomic Analysis of the Response of Funnelifor Mismosseae/Medicago Sativa to Atrazine Stress Xin Sui1,2† ,Qiwu1,2†, Wei Chang1,2, Xiaoxu Fan1,2 and Fuqiang Song1,2*
Sui et al. BMC Plant Biology (2018) 18:289 https://doi.org/10.1186/s12870-018-1492-1 RESEARCH ARTICLE Open Access Proteomic analysis of the response of Funnelifor mismosseae/Medicago sativa to atrazine stress Xin Sui1,2† ,QiWu1,2†, Wei Chang1,2, Xiaoxu Fan1,2 and Fuqiang Song1,2* Abstract Background: Arbuscular mycorrhizal (AM) fungi form symbiotic associations with host plants can protect host plants against diverse biotic and abiotic stresses, and promote biodegradation of various contaminants. However, the molecular mechanisms of how the arbuscular mycorrhizal fungi and host plant association on atrazine stress were still poorly understood. To better characterize how arbuscular mycorrhizal fungi and host plant interactions increase atrazine stress, we performed physiological and proteomic analysis of Funneliformis mosseae (mycorrhizal fungi) and Medicago sativa (alfalfa) association under atrazine stress. Results: The results showed that in the Arbuscular mycorrhizal, protective enzymes were up regulated and the malondialdehyde content increased relative to those of non-mycorrhizal M.sativa. We also examined the atrazine degradation rates within the nutrient solution, and a 44.43% reduction was observed with the mycorrhizal M.sativa, with 30.83% of the reduction attributed to F. mosseae. The accumulation content in root and stem of mycorrhizal M.sativa were obviously increased 11.89% and 16.33% than those of non- mycorrhizal M.sativa. The activity of PPO, POD, CAT and SOD in mycorrhizal M.sativa were obviously higher than non mycorrhizal M.sativa under atrazine stess. We identified differential root proteins using isobaric tags for relative and absolute quantization coupled with liquid chromatography–mass spectrometry, with 533 proteins identified (276 unregulated and 257 downregulated). -
Review Course Lectures
Review Course Lectures International Anesthesia Research Society IARS 2011 REVIEW COURSE LECTURES The material included in the publication has not undergone peer review or review by the Editorial Board of Anesthesia and Analgesia for this publication. Any of the material in this publication may have been transmitted by the author to IARS in various forms of electronic medium. IARS has used its best efforts to receive and format electronic submissions for this publication but has not reviewed each abstract for the purpose of textual error correction and is not liable in any way for any formatting, textual, or grammatical error or inaccuracy. 2 ©2011 International Anesthesia Research Society. Unauthorized Use Prohibited IARS 2011 REVIEW COURSE LECTURES Table of Contents Perioperative Implications of Emerging Concepts In Management of the Malignant Hyperthermia Vascular Aging, Health And Disease Patient In Ambulatory Surgery Charles W. Hogue, MD ..............................1 Denise J. Wedel, MD ...............................38 Professor of Anesthesiology and Professor of Anesthesiology, Mayo Clinic Critical Care Medicine Rochester, Minnesota Chief, Division of Adult Anesthesia The Johns Hopkins University School of Medicine, Central Venous Access Guideline The Johns Hopkins Hospital Development and Recommendations Baltimore, Maryland Stephen M. Rupp, MD ..............................41 Anesthesiologist Perioperative Management of Pain and PONV in Medical Director, Perioperative Services Ambulatory Surgery Virginia Mason Medical Center, Seattle, Washington Spencer S. Liu, MD .................................5 Clinical Professor of Anesthesiology Pediatric Anesthesia and Analgesia Outside the OR: Director of Acute Pain Service What You Need To Know Hospital for Special Surgery Pierre Fiset, MD, FRCPC............................47 New York, New York Department Head, Anesthesiology Montreal Children’s Hospital Colloid or Crystalloid: Any Differences In Outcomes? Montreal, Quebec, Canada Tong J. -
Studies on the Biodegradation of Atrazine
Universidade de Lisboa Faculdade de Ciências Departamento de Biologia Vegetal STUDIES ON THE BIODEGRADATION OF ATRAZINE IN SOILS CONTAMINATED WITH A COMMERCIAL FORMULATION CONTAINING ATRAZINE AND S-METOLACHLOR: SCALE-UP OF A BIOREMEDIATION TOOL BASED ON PSEUDOMONAS SP. ADP AND EVALUATION OF ITS EFFICACY Ana Catarina da Silva Portinha e Costa Mestrado em Biologia Celular e Biotecnologia Setembro 2008 Universidade de Lisboa Faculdade de Ciências Departamento de Biologia Vegetal STUDIES ON THE BIODEGRADATION OF ATRAZINE IN SOILS CONTAMINATED WITH A COMMERCIAL FORMULATION CONTAINING ATRAZINE AND S-METOLACHLOR: SCALE-UP OF A BIOREMEDIATION TOOL BASED ON PSEUDOMONAS SP. ADP AND EVALUATION OF ITS EFFICACY Ana Catarina da Silva Portinha e Costa Mestrado em Biologia Celular e Biotecnologia Dissertação orientada por: Doutora Cristina Anjinho Viegas (Instituto Superior Técnico, Universidade Técnica de Lisboa) Doutora Maria Isabel Caçador (Faculdade de Ciências, Universidade de Lisboa) Setembro 2008 ABSTRACT Atrazine has been used worldwide since 1952 and is frequently detected above the levels established by regulatory authorities in consumption waters. Therefore, and because of its ecotoxicological properties, its use has been forbidden in most European countries, including Portugal. However, atrazine is still used in many countries worldwide. The main purpose of the present work was to examine the efficacy of the atrazine- degrading bacteria Pseudomonas sp. ADP ( P. ADP) as bioaugmentation agent in soils contaminated with high doses (~20x and ~50xRD; RD – Recommended dose) of the commercial formulation, Primextra S-Gold, that contains atrazine, and also S-metolachlor and benoxacor as main active ingredients. It was also tested the effect of combining bioaugmentation and biostimulation using soil amendment with trisodium citrate in open soil microcosms, with the purpose of scaling-up this bioremediation tool. -
Final List of Participants
Final list of participants 1) States and European Community 2) Entities and intergovernmental organizations having received a Standing invitation from the United Nations General Assembly 3) United Nations Secretariat and Organs 4) United Nations Specialized Agencies 5) Associate Members of Regional Commissions 6) Other invited intergovernmental organizations 7) Non governmental organizations (NGOs) and civil society organizations 8) Business Sector Entities 1) STATES AND EUROPEAN COMMUNITY Afghanistan Representatives: H.E. Mr Mohammad M. STANEKZAI, Ministre des Communications, Afghanistan, [email protected] H.E. Mr Shamsuzzakir KAZEMI, Ambassadeur, Representant permanent, Mission permanente de l'Afghanistan, [email protected] Mr Abdelouaheb LAKHAL, Representative, Delegation of Afghanistan Mr Fawad Ahmad MUSLIM, Directeur de la technologie, Ministère des affaires étrangères, [email protected] Mr Mohammad H. PAYMAN, Président, Département de la planification, Ministère des communications, [email protected] Mr Ghulam Seddiq RASULI, Deuxième secrétaire, Mission permanente de l'Afghanistan, [email protected] Albania Representatives: Mr Vladimir THANATI, Ambassador, Permanent Mission of Albania, [email protected] Ms Pranvera GOXHI, First Secretary, Permanent Mission of Albania, [email protected] Mr Lulzim ISA, Driver, Mission Permanente d'Albanie, [email protected] Algeria Representatives: H.E. Mr Amar TOU, Ministre, Ministère de la poste et des technologies -
List of Congress Delegates
List of Congress Delegates № Company Name Surname Position 1 AB "RUSSIA" Pavel Petrovskiy Vice President, Director of corporate business Department 2 AB "RUSSIA" Alexey Vinogradov Head of corporate business corporate business Department 3 Acceleration Management Solutions Angelo Codignoni President 4 Acceleration Management Solutions Morel Jean-Pierre Consultant 5 Acceleration management Solutions Mauro Sipsz Director "Adult Education and Working Life Services 6 Ilya Ouretski Salpaus Further Education" 7 AEM-technology Eugeniy Pakermanov Director General 8 Aeroflot Igor Kozhurov Department of internal control, Director 9 Afrikantov OKB Mechanical Engineering Sergey Dushev Deputy Chief Designer of Fuel Handling Equipment 10 Afrikantov OKB Mechanical Engineering Sergey Fateev Lead Engineer 11 Afrikantov OKB Mechanical Engineering Nadezhda Knyazeva Engineer 12 Afrikantov OKB Mechanical Engineering Feliks Lisitsa Director Consultant "Chief Auditor - the chief of service 13 Afrikantov OKB Mechanical Engineering Lyudmila Manuilova internal control and audit" 14 Afrikantov OKB Mechanical Engineering Evgeniy Naumov Deputy Director for HR Management and Social Issues 15 Afrikantov OKB Mechanical Engineering Sergey Salnikov Head of International Relations and Foreign Economic Activity Department 16 Afrikantov OKB Mechanical Engineering Igor Shmelev Head of Strategic Development and Foreign Economic Activity Division 17 Akdeniz University Muzaffer Karasulu Professor 18 Akkuyu NPP Tahir Agaev PR/GR manager 19 Akkuyu NPP Fuad Ahundov Director General -
Physician Directory Jacobi Health Professional by License And
NYC Health + Hospitals Physician Directory Corporate Finance Jacobi Updated as of March 25, 2016 Health Professional By License and NPI FACILITY NAME DOCTOR LAST NAME DOCTOR FIRST NAME Jacobi AAGAARD PHILIP Jacobi AARON ANDREA Jacobi AARON MIRANDA Jacobi ABADI JACOBO Jacobi ABADI MARIA Jacobi ABADIR DALE Jacobi ABADIRHALLOCK MICHELLE Jacobi ABAPO BLANCA Jacobi ABARCA FRANCISCO Jacobi ABARE MARCE Jacobi ABAYEVA IRINA Jacobi ABBADESSA BENJAMIN Jacobi ABBALEMATTEO DAVID Jacobi ABBAS NAEEM Jacobi ABBASOVA SABINA Jacobi ABBATEMATTEO DAVID Jacobi ABBIATI ROBERT Jacobi ABDELDAYEM HANEEN Jacobi ABDO MUFI Jacobi ABDOU EMAD Jacobi ABDULQUADER MOHAMMED Jacobi ABDURRAHEIM NURI Jacobi ABELARDO DEANDA Jacobi ABELES EDWARD Jacobi ABELES HANS Jacobi ABELL REBECCA Jacobi ABESKHERON JOLLY Jacobi ABIAAD SIMON Page 1 of 262 NYC Health + Hospitals Physician Directory Corporate Finance Jacobi Updated as of March 25, 2016 Health Professional By License and NPI FACILITY NAME DOCTOR LAST NAME DOCTOR FIRST NAME Jacobi ABITBOL AGNES Jacobi ABITBOL AGNES Jacobi ABOAGYE ALEX Jacobi ABOGUNRIN GRACE Jacobi ABORDO ERIKA Jacobi ABRAHAM SAFER Jacobi ABRAHAM BINU Jacobi ABRAHAM AMI Jacobi ABRAMS KIM Jacobi ABRUZZOFOGARASSY MARY Jacobi ABUMEHLHA ADBULAZIZ Jacobi ACEVEDO DORA Jacobi ACEVEDO NATTASHA Jacobi ACHARYA ANJALI Jacobi ACKER JESSICA Jacobi ACKERMAN JEREMY Jacobi ACKERMAN NINA Jacobi ACOSTA NIVIA Jacobi ACOSTA ROBERT Jacobi ACUNAVILLAORDUNA ANA Jacobi ADAMO ARTHUR Jacobi ADAMS HENRY Jacobi ADARAMOLAOJO MOJISOLA Jacobi ADEBANJO OLUGBENGA Jacobi ADEL MEHRON Jacobi ADEOYE -
The Influence of Vegetation, Microbial Inoculation, and Aging of Pesticide
Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 2001 The influence of vegetation, microbial inoculation, and aging of pesticide residues on the degradation of atrazine and metolachlor in soils Shaohan Zhao Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Agriculture Commons, Environmental Sciences Commons, and the Soil Science Commons Recommended Citation Zhao, Shaohan, "The influence of vegetation, microbial inoculation, and aging of pesticide residues on the degradation of atrazine and metolachlor in soils " (2001). Retrospective Theses and Dissertations. 693. https://lib.dr.iastate.edu/rtd/693 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, sbme thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. -
European Youth Olympic Festival X Festival Olympique De La Jeunesse
10th Summer edition of the European Youth Olympic Festival 18–25 July X Festival Olympique de la Jeunesse Européenne Edition d’été 18–25 juillet EUROOPAN NUORTEN OLYMPIAFESTIVAALIT 18.–25.7. EYOF Tampere 2009 is organised by EYOF is owned by and organised In Cooperation with HLU – Regional Event ResultsThe European1 EYOF Tampere 2009 Ministry of City of Tampere Finnish Olympic Committee Sports Federation Olympic Committees Education PARTNERS: Finnish Olympic Committee´s Main Sponsors EYOF SPORTS: Athletics by Tampereen Pyrintö Basketball by Tampereen Pyrintö Cycling by Tampereen Yritys Gymnastics by Tampereen Voimistelijat and Tampereen Sisu Handball by Finnish Handball Association Judo by Tampereen Judo Swimming by Tampereen Uimaseura, KOO-VEE and TaTu Tennis by TATS Volleyball by Isku Volley Event Results 2 EYOF Tampere 2009 RESULTS RÉSULTATS 10th Summer Edition of the European Youth Olympic Festival 2009 X Festival Olympique de la Jeunesse Européenne Edition d’été 2009 Tampere 19 – 24 July 2009 | Tampere 19 – 24 juillet 2009 Sincerely Yours! EYOF TAMPERE 2009 ORGANISING COMMITTEE Pekka P. Paavola Pirjo Puskala Chair Chief Executive INDEX Athletics ..........................................................................................4 Basketball .....................................................................................44 Cycling ........................................................................................48 Gymnastics ...................................................................................52 Handball -
New Aspects on Atrazine Biodegradation
487 Vol.53, n. 2: pp. 487-496, March-April 2010 BRAZILIAN ARCHIVES OF ISSN 1516-8913 Printed in Brazil BIOLOGY AND TECHNOLOGY AN INTERNATIONAL JOURNAL New Aspects on Atrazine Biodegradation Luciane Sene 1*, Attilio Converti 2, Geslaine Aparecida Ribeiro Secchi 1 and Rita de Cássia Garcia Simão 1 1Centro de Ciências Médicas e Farmacêuticas; Universidade Estadual do Oeste do Paraná; Rua Universitária 2069; Cascavel - PR - Brasil. 2Facoltà di Ingegneria; Dipartimento di Ingegneria Chimica e di Processo; Università degli Studi di Genova; Via dell’Opera Pia 1; I-16145; Genoa - Italy ABSTRACT The world practice of using agrochemicals for long periods, in an indiscriminated and abusive way, has been a concern of the authorities involved in public health and sustainability of the natural resources, as a consequence of environmental contamination. Agrochemicals refer to a broad range of insecticides, fungicides and herbicides, and among them stands out atrazine, a herbicide intensively used in sugarcane, corn and sorghum cultures, among others. Researches have demonstrated that atrazine has toxic effects in algae, aquatic plants, aquatic insects, fishes and mammals. Due to the toxicity and persistence of atrazine in the environment, the search of microbial strains capable of degrading it is fundamental to the development of bioremediation processes, as corrective tools to solve the current problems of the irrational use of agrochemicals. This review relates the main microbial aspects and research on atrazine degradation by isolated microbial species and microbial consortia, as well as approaches on the development of techniques for microbial removal of atrazine in natural environments. Key words : atrazine, biodegradation, bacteria, fungi, microbial consortium INTRODUCTION thus, inhibiting the photosynthetic electron transport.