P983 Abstract (poster session) Prevalence of Chlamydophila pneumoniae in tissue of children with chronic adenoiditis E. Podsiadly*, A. Bielicka, B. Zielnik-Jurkiewicz, U. Demkow (Warsaw, PL)

The aim of the study was to determine the presence of C. in adenoid tissue and estimate risk factor of C. pneumonia infection in children with chronic adenoiditis. Two hundred consecutive children aged 2-16 years undergoing planned between 02.2010 and 05.2011 were enrolled to the study. Eligibility criteria: upper airways obstruction, caused by adenoid hypertrophy and/or with chronic adenoiditis. were analysed for the presence of C. pneumonia DNA by real-time PCR on a LightCycler® 2.0 with LightMix® Kit Chlamydophila pneumoniae (Tib Molbiol GmbH, Germany). DNA from a tissue was isolated with High Pure PCR Kit (Roche Diagnostics, Germany). Smears from the adenoids were cultured on bacteriological media. Additionally, adenoid tissue samples from 22 patients were examined with immunohistochemical methods with application of monoclonal anti-C. pneumoniae antibodies (Thermo Scientific) for the presence of chlamydia. C. pneumoniae DNA in the adenoid was present in 11 children (5,5%). C. pneumoniae DNA was detected most frequently (24,1%) in children 10-16 years old. Girls were infected more often than boys 63,6% vs 36,26%. No statistically significant differences were observed between PCR (+) and PCR (-) children as a result of, a , recurrent respiratory infections, , an , atopic diseases and frequency of antibiotic courses. In PCR (+) children more frequently (54,5%) than in PCR negative children (14,2%) poor standard of living was observed (p<0,01). C. pneumonia PCR (+) children tend to have prolonging >= 3 weeks (90,9% vs 68,7%). Interestingly, C. pneumoniae DNA was detected in one case in adenoid removed from siblings. In nine out of 11 (81,82%) C. pneumoniae PCR (+) children other pathogenic were detected: S.pneumoniae (4), S. aureus (4), S. pyogenes (3) and H. influenzae (2). In two of the PCR (+) patients no co-pathogens were found. Results of immunohistochemical staining showed presence of C. pneumoniae in lymphocytes and epithelium of analysed tonsils. Existence of C. pneumoniae in an adenoid tissue suggest participation of the bacteria in adenoid hypertrophy. However, comparison of results of bacteriological culture from adenoid swabs with PCR results suggests co-participation of C. pneumonia in pathogenesis rather than decisive part. This etiology specially concerns school children who are more prone to C. pneumoniae infections.