Evidence-based practice How much UV-B does my reptile need? The UV-Tool, a guide to the selection of UV lighting for reptiles and amphibians in captivity Frances Baines1*, Joe Chattell2, James Dale3, Dan Garrick4, Iri Gill5, Matt Goetz6, Tim Skelton7 and Matt Swatman3 1UV Guide UK, Abergavenny, UK 2Reaseheath College, Nantwich, UK 3Chester Zoo, UK 4Marwell Zoo, UK 5Zoological Society of London, UK 6Durrell Wildlife Conservation Trust, Jersey 7Bristol Zoo Gardens, UK JZAR Evidence-based practice Evidence-based JZAR *Correspondence: Frances Baines, UV Guide UK, Greenfield, School Lane, Govilon, Abergavenny NP7 9NT, UK; [email protected] Keywords: Abstract microhabitat design, UV-B, UV index, Guidance is almost non-existent as to suitable levels of UV lighting for reptiles and amphibians, or UV lamps, UV requirements, vivarium how to achieve satisfactory UV gradients using artificial lighting. The UV-Tool is a working document lighting that seeks to address this problem, by considering the range of UV experienced by each species in the wild. The UV-Tool contains an editable and expanding database of the microhabitat requirements and Article history: basking behaviour of reptile and amphibian species, as derived from field studies, or inferred from Received: 9 July 2015 observed behaviour in captivity. Since an animal’s UV-B exposure is determined by its behaviour within Accepted: 15 January 2016 its native microhabitat, estimation of its natural range of daily UV-B exposure is then possible. The Published online: 31 January 2016 current version of the UV-Tool assigns 254 species to each of four ‘zones’ of UV-B exposure (Ferguson zones) based upon UV-index measurements. Once the likely UV requirement of any species of reptile or amphibian is ascertained, the next step is to plan safe but effective UV gradients within the captive OPEN ACCESS environment. To do this requires knowledge of the UV spectrum and output of the lamps to be used. The UV-Tool therefore includes test reports and UV-index gradient maps for commercially available UV-B lighting products, and a guide to selection of appropriate lamps for use in vivaria and in larger zoo enclosures. There are reports on 24 different products in the current version of the UV-Tool. This document has been compiled by members of the British and Irish Association of Zoos and Aquaria (BIAZA) Reptile and Amphibian Working Group (RAWG) with contributions from zookeepers and herpetologists from the UK and abroad. Further input is welcome and encouraged. Introduction from infra-red to ultraviolet (UV) may be utilised by these animals, and are received in amounts that depend upon their The provision of UV lighting to captive reptiles and amphibians microhabitat and their daily activity patterns. Ultraviolet is a is widely recommended (e.g. Rossi 2006; Carmel and Johnson normal component of sunlight. It is subdivided by wavelength; 2014; Tapley et al. 2015). However, guidance as to suitable levels natural sunlight consists of a short-wavelength fraction, UV-B of UV-B for different species, and how to achieve satisfactory (290–320 nm) and a longer-wavelength fraction, UV-A (320– UV gradients, is almost non-existent. The aim of this project 400 nm). is to create a working document that can be used as a guide UV-A from around 350 nm is within the visual range of many to suitable UV lighting for all reptiles and amphibians kept in reptiles and amphibians, which use it in recognising conspecifics captivity. The project was initiated by the UV Focus Group of and food items (Govardovskii and Zueva 1974; Moehn 1974; the British and Irish Association of Zoos and Aquaria (BIAZA) Fleishman et al. 1993; Honkavaara et al. 2002); its provision Reptile and Amphibian Working Group (RAWG). within the spectrum is therefore very important. Every aspect of the life of a reptile or amphibian is governed Short-wavelength UV-B (290–315nm) enables the conversion by its daily experience of solar light and heat – or the artificial of 7-dehydrocholesterol (7DHC), a sterol in the skin, to pre- equivalent, when it is housed indoors. All wavelengths vitamin D3. In skin this undergoes a temperature-dependent Journal of Zoo and Aquarium Research 4(1) 2016 42 A UV-B lighting guide for reptiles and amphibians isomerisation into vitamin 3D , which is metabolised by the liver 1988, 1989, 1991; Hertz et al 1994; Dickinson and Fa 1997) and and subsequently by the kidney into the vital endocrine hormone in some cases for UV photoregulation (Manning and Grigg 1997; calcitriol, controlling calcium metabolism. It is also metabolised into Ferguson et al. 2003; Karsten et al. 2009). The animal’s response calcitriol intracellularly throughout the body, where in mammals will determine its exposure within these gradients. Variation in it has been shown to perform multiple autocrine and paracrine behaviour creates enormous differences in UV exposure between functions, controlling transcription of as many as 2000 genes species, ranging from mid-day full-sun baskers to nocturnal and that influence functions as diverse as growth, insulin production crepuscular animals, which may receive the majority of their and the immune system (Hossein-nezhad and Holick 2013). ultraviolet exposure from small amounts of daylight reaching Overproduction of vitamin D3 is prevented by the conversion of them in their diurnal retreats. excess pre-vitamin D3 and vitamin D3 into inert photoproducts by The creation of similar superimposed heat, light and UV UV-B and short-wavelength UV-A (range 290–335nm), effectively gradients using UV-B-emitting lamps, often in combination making this natural process, in sunlight, self-limiting (MacLaughlin with other sources of heat and light, is possible because their et al. 1982; Webb et al. 1989). Although most research on vitamin irradiance is proportional to the distance from the lamp. The task D3 has been carried out on mammals, studies conducted on other requires knowledge of (1) the range of irradiance appropriate for taxa indicate that vitamin D pathways are similar in most terrestrial the species and (2) the gradients created by individual lighting vertebrates (Holick et al. 1995; Bidmon and Stumpf 1996; Antwis products, which may be used individually or in combination to and Browne 2009). produce the desired effect. As well as its role in enabling and regulating cutaneous vitamin D synthesis, UV has direct effects upon skin, which include The range of irradiance appropriate for the species modulation of the cutaneous immune system, strengthening of Research on this topic is in its infancy, even with regard to skin barrier functions and increasing pigment formation. It also human beings. There is hardly any scientific data to back the stimulates production of beta endorphins, giving sunlight its recommendation of any particular level of UV-B for any particular ‘feel good’ factor, and induces nitric oxide production, which has species. Until very recently, no practical methods existed for localised protective effects (Juzeniene and Moan 2012). Solar UV recording ambient UV-B in the microhabitat of free-living reptiles is also an effective disinfectant (McGuigan et al. 2012) that can and amphibians. However, Ferguson et al. (2010) reported the UV destroy bacteria, fungi and viruses on the surface of the skin. exposure of 15 species of reptiles in the field during their daily Excessive exposure to UV must, however, be avoided. High and seasonal peak of activity, using the unitless UV index (UVI), doses and/or exposure to unnaturally short-wavelength UV from as measured with a Solarmeter 6.5 UV Index meter (Solartech artificial sources can result in eye and skin damage, reproductive Inc., Harrison Township, Michigan, USA), and demonstrated failure or even the death of amphibians (Blaustein and Belden that knowledge of the basking/daylight exposure habits of any 2003) and reptiles (Gardiner et al. 2009), and in mammals, can species enables a reasonable estimation of likely UV exposures to lead to the formation of skin cancers (Soehnge 1997). Squamous be made. They allocated species into four sun exposure groups cell carcinomas have been reported in captive reptiles but the or ‘zones’, which have since been designated ‘UV-B Zones’ or significance of their association with the use of artificial UV ‘Ferguson zones’ (Carmel and Johnson 2014; Ferguson et al. 2014). lighting is as yet undetermined (Duarte and Baines 2009; Hannon For each zone, a range of figures was given for the mean voluntary et al. 2011). UVI exposures calculated from all readings (Zone Range), and for Species vary widely in their basking behaviours or lack of them the maximum UVI in which the animals were encountered. The (Avery 1982; Tattersall et al. 2006; Michaels and Preziosi 2013), Ferguson zones are summarised in Table 1. their skin permeability to UV radiation (Porter 1967; Nietzke 1990) Any species can be assigned to one of the four zones based and in their response to UV-B in terms of vitamin D3 production upon its basking behaviour. The authors suggest that a suitable UV (Carman et al. 2000). These behavioural and morphological gradient may then be provided in the captive animal’s environment characteristics optimise their UV exposure for vitamin D synthesis using these figures as a guide. Such a gradient should enable the and the other beneficial effects of sunlight, whilst simultaneously animal to self-regulate its exposure from zero (full shade) to the minimising the risk of UV damage, but these adaptations are only maximum indicated for that zone, which would be provided at the relevant for the solar irradiation they experience in their native animal’s closest access point to the lamp. microhabitat. Thus it would seem very important to match the solar UV spectrum as closely as possible, and to recreate the levels of irradiance found in this microhabitat, when providing reptiles and amphibians with artificial lighting.