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Dissertation Final DEVELOPMENT AND APPLICATION OF MANAGEMENT FOR FOUR SHRIMP DISEASES (TSV, YHV, WSSV AND NHP) IN THE WHITE SHRIMP Penaeus vannamei THROUGH DIFFERENT STRATEGIES Item Type text; Electronic Dissertation Authors Aranguren, Luis Fernando Publisher The University of Arizona. Rights Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. Download date 29/09/2021 12:17:45 Link to Item http://hdl.handle.net/10150/205231 1 DEVELOPMENT AND APPLICATION OF MANAGEMENT FOR FOUR SHRIMP DISEASES (TSV, YHV, WSSV AND NHP) IN THE WHITE SHRIMP Penaeus vannamei THROUGH DIFFERENT STRATEGIES by Luis Fernando Aranguren Caro Copyright © Luis Fernando Aranguren Caro 2011 A Dissertation Submitted to the Faculty of the DEPARTMENT OF VETERINARY SCIENCE AND MICROBIOLOGY In Partial Fulfillment of the Requirements For the Degree of DOCTOR OF PHILOSOPHY WITH A MAJOR IN MICROBIOLOGY AND PATHOBIOLOGY In the Graduate College THE UNIVERSITY OF ARIZONA 2011 2 THE UNIVERSITY OF ARIZONA GRADUATE COLLEGE As members of the Dissertation Committee, we certify that we have read the dissertation prepared by Luis Fernando Aranguren Caro entitled: Development and application of management for four shrimp diseases (TSV, YHV, WSSV and NHP) in the white shrimp Penaeus vannamei through different strategies and recommend that it be accepted as fulfilling the dissertation requirement for the Degree of Doctor of Philosophy __________________________________________________ Date: September 29, 2011 Donald V. Lightner, Ph.D. __________________________________________________ Date: September 29, 2011 Beth Jacobs, Ph.D. __________________________________________________ Date: September 29, 2011 Kathy F.J Tang-Nelson, Ph.D. __________________________________________________ Date: September 29, 2011 David Besselsen, Ph.D. __________________________________________________ Date: September 29, 2011 Carlos Reggiardo, Ph.D. Final approval and acceptance of this dissertation is contingent upon the candidate’s submission of the final copies of the dissertation to the Graduate College. I hereby certify that I have read this dissertation prepared under my direction and recommend that it be accepted as fulfilling the dissertation requirement. __________________________________________________ Date: September 29, 2011 Dissertation Director: Dr. Donald V. Lightner 3 STATEMENT BY AUTHOR This dissertation has been submitted in partial fulfillment of requirements for an advanced degree at The University of Arizona and is deposited in the University Library to be made available to borrowers under rules of the library. Brief quotations from this dissertation are allowable without special permission, provided that accurate acknowledgement of source is made. Requests for permission for extended quotation from or reproduction of this manuscript in whole or in part may be granted by the copyright holder. Luis Fernando Aranguren Caro 4 ACKNOWLEDGEMENTS I would like to thanks the Colombian Corporation Center for Research in Aquaculture CENIACUA and The “Departamento Administrativo de Ciencia, Tecnología y Educación” COLCIENCIAS for providing me the scholarship award for my graduate studies at the University of Arizona. I would like to thank my mentor and major advisor, Dr. Donald V. Lightner, for allowing me to become part of the Aquaculture Pathology Laboratory for these 5 years and for his endless patience, and for providing me valuable advice and feedback. To my graduate committee members: Dr. Kathy Tang-Nelson, Dr. Beth Jacobs, Dr. Carlos Reggiardo and Dr. David Besselsen. Your guidance, teaching, and advice have been essential in completing this work. I would like to thank to Dr. Chuck Sterling and Liz Gradillas in the Department of Veterinary Sciences and Microbiology for providing graduate teaching assistantships. I would like to thanks Marcela Salazar for her guidance with my research. Special thanks are extended to lab members: Linda Nunan, Dr. Carlos Pantoja, Leone Mohney, Rita Redman, Solangel Navarro, Brenda Noble and Paul Schofield for their assistance, teaching and patience. I thank fellow graduate students, past and present: Thales Andrade, Lisbeth Echavarria and Allan Heres for their friendship and encouragement. 5 DEDICATION To my parents: Augusto and Elsa de Aranguren for giving me their love and living experiences To my wife: Yessenia, my sons Andrés and Lucas and my daughter Juliana, for being the inspiration every day To my siblings Augusto and Monica for being more that a brother and a sister, my best friends To shrimp 6 TABLE OF CONTENTS LIST OF FIGURES .......................................................................................................8 LIST OF TABLES...........................................................................................................10 ABSTRACT......................................................................................................................11 CHAPTER 1. INTRODUCTION ..........................................................................12 Literature review .........................................................................................12 Description of etiological agents used in this research ...............................13 Strategies for diseases management ...................................................................16 Strategy for TSV management ..........................................................................16 Strategy for YHV management ...................................................................18 Strategy for WSSV management ...................................................................19 Strategy for NHP management ..........................................................................21 CHAPTER 2. CHARACTERIZATION OF A HIGHLY PATHOGENIC STRAIN OF TAURA SYNDROME VIRUS (TSV) FROM COLOMBIA SHRIMP CULTURE ........................................................................................................................23 Abstract .....................................................................................................23 Introduction........................................................................................................24 Materials and Methods.......................................................................................27 Results................................................................................................................32 Discussion..........................................................................................................38 Acknowledgments..............................................................................................43 Tables and Figures.............................................................................................44 CHAPTER 3. PROTECTION FROM YELLOW HEAD VIRUS (YHV) INFECTION IN Penaeus vannamei PRE-INFECTED WITH TAURA SYNDROME VIRUS (TSV) .............................................................................................................49 Abstract ...................................................................................................49 Introduction ....................................................................................................50 Materials and Methods .................................................................................53 Results ....................................................................................................57 Discussion ....................................................................................................59 Acknowledgments.............................................................................................63 Tables and Figures ……...................................................................................65 7 TABLE OF CONTENTS - Continued CHAPTER 4. USE OF A MARINE BACTERIUM (Vibrio sp.) AS A DELIVERY VEHICLE FOR WSSV VP28 GENE INTO SHRIMP Penaeus vannamei............69 Abstract.............................................................................................................69 Introduction.......................................................................................................70 Materials and Methods......................................................................................73 Results...............................................................................................................79 Discussion.........................................................................................................81 Acknowledgments.............................................................................................84 Tables and Figures............................................................................................85 CHAPTER 5. QUANTIFICATION OF THE BACTERIAL AGENT OF NECROTIZING HEPATOPANCREATITIS (NHP-B) BY REAL-TIME PCR AND COMPARISON OF SURVIVAL AND NHP LOAD OF TWO SHRIMP POPULATIONS ...................................................................................................91 Abstract..................................................................................................................91 Introduction............................................................................................................92 Materials and Methods...........................................................................................94
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