Chicken Gut Microbiota – towards a new generation of probiotics in poultry

Ivan Rychlik

Veterinary Research Institute, Brno, Czech Republic

Discover µworld Development of chicken caecal microbiota

100 15 16 14 G- 1 - Bifidobacteriaceae 10 13 90 12 2 - Bacteroidaceae 3 - Porphyromonadaceae 80 11 9 G+ 4 - Prevotellaceae 70 5 - Rikenellaceae 60 6 - unclassified Bacteroidales 7 - microbiota 50 7 6 8 - Lachnospiraceae total 40 4 9 - Ruminococcaceae

of of 10 - unclassified Clostridiales 30 5 % % 8 G- 11 - Veillonellaceae 20 12 - Lactobacillaceae 3 13 – Acidaminococcaceae 10 5 2 14 – Fusobacteriaceae 0 1 G+ 15 - Desulfovibrionaceae 0 10 20 30 40 50 60 16 – Enterobacteriaceae age of chickens (weeks)

Discover µworld Videnska et al. Succession and replacement of bacterial populations in the caecum of egg laying hens over their whole . PLoS One 2014;9:e115142. Development of chicken caecal microbiota 100 80

60 microbiota

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Kubasova et al. Contact with adult hen affects development of caecal microbiota in newly hatched chicks. PLoS One 2019;14:e0212446.

Rychlik. Composition and Function of Chicken Gut Microbiota. 2020;10(1). log CFU/ g Chickens Varmuzova Microbiota Control to etal. Salmonella Composition Contact L Enteritidis iver and WCHA of Gut Gut of Infection Salmonella Microbiota YCFA . Front Influences

log CFU/ g Microbiol Control resistance Resistance 2016;7:957 Contact Spleen of of . Newly WCHA Hatched YCFA WCHA

log CFU/ g Control Contact YCFA Caecum WCHA YCFA ATTGCGCGAAGTGTGAAAGCGCGAGGATGTAC ACAGTCCACAGCACGAGAGCTCTCCGTGTGCT CGCCTGTCGCTCGCTCGTATATAATGATCATCAT CATCAGTCGATACATTATAATCCTTGCGCCTC

Crhanova et al. Systematic Culturomics Shows Medvecky et al. Whole genome sequencing and that Half of Chicken Caecal Microbiota function prediction of 133 gut anaerobes isolated Members can be Grown in Vitro Except for from chicken caecum in pure cultures. BMC Two Lineages of Clostridiales and a Single Genomics 2018;19:561 Lineage of Bacteroidetes. Microorganisms 2019;7(11). Clostridium IV Veillonellaceae

Clostridium XlVb Ruminococcaceae

Clostridium XlVa Lachnospiraceae

Fusobacteria

Bacteroidaceae Erysipelotrichaceae

Clostridium XVIII

Prevotellaceae Lactobacillaceae Porphyromonadaceae Rikenellaceae Kubasova et al. Gut Anaerobes Capable of Chicken Caecum Colonisation. Microorganisms 2019;7(12). % of total microbiota Ability of tested gut anaerob es to colonise chicken caecum Discover µ world Real time PCR quantification of Salmonella Enteritidis in chickens colonised by different defined mixtures of gut anaerobes

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Salmonella 0.0000001 % % 0.00000001 Farm testing 10 800 female chickens of broiler parents 7 500 female chickens of broiler parents + 7 500 male chickens of broiler parents 2 x 7 500 female chickens of broiler parents 18 800 broilers 18 800 broilers 18 800 broilers Death rates

BP1 BP2a BP2b

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BP3b B1-3

BP3a (%)

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Probio Control Colonisation rate

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% % % % Discover µworld Day 1-3, specific fermented feed, day 7 challenge, day 11 bacterial counts

Salmonella Salmonella Campylobacter 9 Caecum 4.5 Liver 12 Caecum 8 4.0 10

7 3.5

g g g 6 3.0 8 5 2.5 6

4 2.0

log log CFU/ log log CFU/ log log CFU/ 3 1.5 4 2 1.0 2 1 0.5 0 0 0 fermented liquid CTRL fermented liquid CTRL fermented liquid CTRL BPVLC BPVLC BPVLC BPVLC BPVLC BPVLC

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There is no (consensual) age dependent microbiota development in chickens.

Bacteroidetes, Veillonellaceae and particular Proteobacteria are efficiently transferred from hens to offspring – natural, environmental, sources are limited.

Chicken gut anaerobes can be obtained in pure cultures and their defined mixtures can be used to increase chicken resistance to Salmonella or Campylobacter.

Mode and time of probiotic administration may affect its efficacy, under field conditions in particular.

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Manipulace se složením střevní mikroflóry monogastrů jako cesta k potlačení E. coli infekcí a snížení spotřeby antimikrobik, QK1810462, Probiom

Innovations in Poultry Medicine, ATCZ194, INPOMED

Probiotické baktérie střevní mikroflóry jako základ zdraví a pohody zvířat CZ.02.1.01/0.0/0.0/16_025/0007404 Enorma timonensis Mediterranea massiliensis

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Drancourtella massiliensis Bacteroides dorei

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