NSUN2 Cat. No.: 55-438

NSUN2 Antibody

Western blot analysis in mouse spleen tissue lysates (35ug/lane).

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse

This NSUN2 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 423-451 amino acids from the Central region of human NSUN2.

TESTED APPLICATIONS: WB

APPLICATIONS: For WB starting dilution is: 1:1000

PREDICTED MOLECULAR 86 kDa WEIGHT:

September 27, 2021 1 https://www.prosci-inc.com/nsun2-antibody-55-438.html Properties

This antibody is purified through a A column, followed by peptide affinity PURIFICATION: purification.

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: NSUN2

tRNA (cytosine(34)-C(5))-, Myc-induced SUN domain-containing protein, Misu, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, ALTERNATE NAMES: tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolog, hTrm4, NSUN2, SAKI, TRM4

ACCESSION NO.: Q08J23

PROTEIN GI NO.: 148887180

GENE ID: 54888

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA . In tRNA- leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a BACKGROUND: modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).

REFERENCES: 1) Bailey, S.D., et al. Diabetes Care (2010) In press :

2) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

3) Frye, M., et al. Cancer Lett. 289(1):71-80(2010)

September 27, 2021 2 https://www.prosci-inc.com/nsun2-antibody-55-438.html 4) Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

ANTIBODIES FOR RESEARCH USE ONLY.

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