NSUN2 Antibody Cat. No.: 55-438
NSUN2 Antibody
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human, Mouse
This NSUN2 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 423-451 amino acids from the Central region of human NSUN2.
TESTED APPLICATIONS: WB
APPLICATIONS: For WB starting dilution is: 1:1000
PREDICTED MOLECULAR 86 kDa WEIGHT:
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This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification.
CLONALITY: Polyclonal
ISOTYPE: Rabbit Ig
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION: batch dependent
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: NSUN2
tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein, Misu, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, ALTERNATE NAMES: tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolog, hTrm4, NSUN2, SAKI, TRM4
ACCESSION NO.: Q08J23
PROTEIN GI NO.: 148887180
GENE ID: 54888
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA- leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a BACKGROUND: modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).
REFERENCES: 1) Bailey, S.D., et al. Diabetes Care (2010) In press :
2) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
3) Frye, M., et al. Cancer Lett. 289(1):71-80(2010)
September 27, 2021 2 https://www.prosci-inc.com/nsun2-antibody-55-438.html 4) Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
ANTIBODIES FOR RESEARCH USE ONLY.
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