Polyclonal Antibody to DLK1 - Aff - Purified

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AP51272PU-N Polyclonal Antibody to DLK1 - Aff - Purified

Alternate names: DLK, DLK-1, PREF1, Preadipocyte factor 1, Protein delta homolog 1, pG2 Quantity: 0.4 ml Concentration: 0.5 mg/ml (Lot specific) Background: This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. Uniprot ID: P80370 NCBI: NP_003827 GeneID: 8788 Host / Isotype: Rabbit / Ig Immunogen: Recombinant protein of Human DLK Genename: DLK1 Format: State: Liquid purified Ig fraction Purification: Purified through a Protein A column followed by peptide affinity purification Buffer System: PBS with 0.09% (W/V) Sodium Azide Applications: Immunohistochemistry on Paraffin Sections: 1/50-1/100. Western blot: 1/1000. Enzyme immunoassay: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody recognizes Human DLK1. Other species not tested. Add. Information: Molecular Weight: 41300 Da Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Yanai, H. et al., J. Biochem. 148(1): 85-92 (2010). 2. Yu, F. et al., Liver Int. 30(5): 703-714 (2010). 3. Akiyama, J. et al., Biochem. Biophys. Res. Commun. 393(4): 662-667 (2010). 4. Wermter, A.K. et al., Eur. J. Hum. Genet. 16(9): 1126-1134 (2008). 5. Charlier, C. et al., Genome Res. 11(5): 850-862 (2001).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20161116 1 / 2 AP51272PU-N: Polyclonal Antibody to DLK1 - Aff - Purified

Pictures: Immunohistochemistry analysis in human placenta tissue (Formalin-fixed, Paraffin-embedded) using DLK1 antibody Cat.-No. AP51272PU-N, followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of DLK1 antibody for IHC. Clinical relevance has not been evaluated.

Western blot analysis in CEM cell line lysates (35ug/lane) using DLK1 antibody Cat.-No. AP51272PU-N. This demonstrates the DLK1 antibody detected the DLK1 protein (arrow).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20161116 2 / 2