Instant FAMETM for Sherlock

Culture to Identification in Less than 15 Minutes

3 Minute Sample New Instant FAME Sample Preparation Method Preparation Sherlock Instant FAME is a new sample preparation method for the Sherlock® Microbial Identification System. This new procedure takes 2-3 minutes to prepare an extract from 3mg of pure culture. Once the Less Than 3mg extract is prepared, less than 9 minutes is required to ID the isolate. of Cells Needed The Sherlock® Microbial Identification System Clinical and environmental laboratories worldwide have used the Sherlock® Microbial Identification System since 1985. The Sherlock Inexpensive Per System identifies microbes by analyzing the fatty acids present in the Sample Cost cell membrane. The fatty acids are separated from the cell and then analyzed with an Agilent Technologies gas chromatograph.

Same Procedure for The Sherlock pattern recognition software compares the fatty acid Bacteria and Yeast pattern of each sample to libraries of stored patterns to make an identification. Additional software tools allow for strain tracking, custom library generation, 21CFR Part 11 compliance and DNA sequencing analyses. Libraries for Clinical, Environmental and The Instant FAME libraries are unique to the Instant FAME sample Water Isolates preparation. Instant FAME libraries cannot be compared to libraries that use the traditional 2-hour MIDI sample preparation.

Instant FAME Identification Procedure

Methylation Extraction Separation

# 1 # 2 # 3

3 mg Cells Vortex Vortex Top Layer

9 min

3 min Analysis Identification

Less Than 15 min Benefits Compared to Other Microbial Identification Systems

Rapid sample preparation and analysis - The Instant FAME identification process takes less than 15 minutes from pure culture to identification, compared to 4-24 hours required of other phenotypic ID systems.

Increased sensitivity - The Instant FAME procedure uses less than 3 mg of cells, compared to the 40mg of cells needed with other identification systems.

Low cost per sample - The Instant FAME cost per sample is 50% less than biochemical-based ID systems and 75% less than DNA sequence-based ID systems.

Standardized procedure for all microbes - Aerobic bacteria and yeast isolates are prepared using the same procedure, so there are no biochemical cards or offline tests required. Benefits Compared to the Traditional MIDI Sherlock Sample Preparation

Reduced extraction time - The entire extraction procedure is reduced from 2 hours to 3 minutes because of a novel fatty acid-based chemistry developed by MIDI. The cells can typically be harvested from the first subculture, unlike the traditional method, which requires 2 subcultures.

Reduced volume of reagents used - There are 3 reagents used during the Instant FAME procedure and only 250ul of each reagent is required. Less than 1ml of waste is generated with each extract.

Less corrosive reagents used - The reagents used are less corrosive. MTBE, a ground water and soil contaminant, is no longer a reagent used within this extraction procedure.

No more water baths, laboratory shakers or chemical fume hoods - The Instant FAME procedure does not require water baths or laboratory shakers like the traditional method does. Depending on the laboratory SOPs, the use of chemical fume hoods may not be necessary because of the extremely small volume of solvents now used with the Instant FAME procedure.

Standard media and growth temperatures used - Aerobic libraries were developed under the following media/growth requirements: o Environmental bacteria library (ITSA1): grown on TSA Agar at 30C. o Clinical bacteria library (IBA1): grown on TSA Agar with 5% defibrinated sheep blood at 35C. o Water bacteria library (IR2A1): grown on R2A Agar at 30C. o Yeast library (ITY1): grown on TSA Agar at 30C.

Improved Entries with updated taxonomy - Up-to-date taxonomy was used to develop the Instant FAME library entries.

Reduced wear on the GC liner - GC liners last twice as long as they did in the 2-hour standard extraction procedure. Specifications

Instant FAME Start Up Kit Contents

 Aerobic Environmental Library (ITSA) – Bacteria grown on TSA Agar at 30C  Aerobic Clinical Library (IBA) – Bacteria grown on TSA Agar with 5% defibrinated sheep blood at 35C  Aerobic Water Isolate Library (IR2A) – Bacteria grown on R2A Agar at 30C  Yeast Library (ITY) – Yeast grown on TSA Agar at 30C  3 liquid dispensers  Vortexer mixer  2 vial rack trays  1 Eppendorf Reference Pipette  100 Glass Spring Foot Inserts  200 Clear 2ml GC Vials  200 PTFE/Silicon/PTFE caps  3 Reagents used for the Fast Extraction shipped in Qorpack bottles  Wash Reagents (Methanol/Hexane)  100 - 1ul sterile inoculation loops  96 Eppendorf pipette tips  1 MIDI pipetting aid  1 GC Column liners (MIDI Part # 1221 / 1221-F for flip top GC)  1 Ampoule Rapid Calibration Mix (MIDI Part # 1300-AA)

Instant FAME Refill Kit Contents – 2 sizes available: 96 samples and 400 samples

 Glass Spring Foot Inserts  Clear 2ml GC Vials  PTFE/Silicon/PTFE caps  3 Reagents used for the Fast Extraction shipped in Qorpack bottles  Wash Reagents (Methanol/Hexane)  1ul sterile inoculation loops  Eppendorf pipette tips  GC Column liners (MIDI Part # 1221 / 1221-F for flip top GC)  Rapid Calibration Mix (MIDI Part # 1300-AA)

Customer Required Items  Sherlock® Microbial Identification System version 6.0 or higher  30C Incubator for Environmental Bacteria (ITSA), Water Bacteria (IR2A) and Yeast (ITY) Libraries  35C Incubator for Clinical Bacteria (IBA1) Library  BBL Brand Trypticase Soy Agar – prepared plates (for ITSA and ITY Libraries)  Difco Brand R2A Agar – dehydrated media (for IR2A Library)  BBL Brand Trypticase Soy Agar with 5% defibrinated sheep blood – prepared plates (for IBA Library) Customer Required Items (continued)

 Petri dishes- 100mm x 15mm (for IR2A Library)  Pasteur pipettes, 5in., disposable  Chemical storage cabinets (for corrosives and flammables)  Hazardous chemical waste disposal container  Hazardous medical waste disposal container  Chemical fume hood (may or may not be necessary depending on SOPs) Sherlock Instant FAME Libraries – 2009

Genus-species IBA1 IR2A1 ITSA1 The following Instant FAME libraries Afipia-felis    are part of the Instant FAME Startup Kit: Aggregatibacter-aphrophilus    Alcaligenes-faecalis    1. IBA1 Instant Clinical Blood Agar library Alloiococcus-otitis    (35°C), version 1.10 Amycolatopsis-orientalis    Aneurinibacillus-aneurinilyticus    2. ITSA1 Instant Environmental TSA library Arcanobacterium-haemolyticum    (30°C) version 1.10 Arcanobacterium-pyogenes    Arcobacter-cryaerophilus    3. IR2A1 Instant Environmental R2A library Arthrobacter-atrocyaneus    (30°C) version 1.10 Arthrobacter-aurescens    Arthrobacter-citreus    4. ITY1 Instant Environmental TSA Yeast Arthrobacter-crystallopoietes    library (30°C) version 1.00 Arthrobacter-globiformis    Arthrobacter-ilicis    Arthrobacter-oxydans    Arthrobacter-pascens    Genus-species IBA1 IR2A1 ITSA1 Arthrobacter-species    Acetobacter-aceti    Avibacterium-avium    Achromobacter-xylosoxidans    Bacillus-atrophaeus    Acidovorax-avenae    Bacillus-badius    Acidovorax-delafieldii    Bacillus-cereus    Acidovorax-facilis    Bacillus-circulans    Acidovorax-temperans    Bacillus-coagulans    Acinetobacter-baumannii    Bacillus-firmus    Acinetobacter-calcoaceticus    Bacillus-flexus    Acinetobacter-genomospecies 9    Bacillus-freudenreichii"    Acinetobacter-haemolyticus    Bacillus-fusiformis    Acinetobacter-johnsonii    Bacillus-gibsonii    Acinetobacter-junii    Bacillus-lentus    Acinetobacter-lwoffii    Bacillus-licheniformis    Acinetobacter-radioresistens    Bacillus-longisporus"    Actinobacillus-equuli    Bacillus-marisflavi    Actinobacillus-lignieresii    Bacillus-megaterium    Actinobacillus-seminis    Bacillus-mycoides    Actinobacillus-suis    Bacillus-oleronius    Actinobacillus-ureae    Bacillus-pumilus    Aerococcus-viridans    Bacillus-simplex    Aeromonas-caviae    Bacillus-sphaericus    Aeromonas-eucrenophila    Bacillus-subtilis    Aeromonas-hydrophila    Bacillus-thuringiensis    Aeromonas-ichthiosmia    Bartonella-henselae    Aeromonas-jandaei    Bergeyella-zoohelcum    Aeromonas-salmonicida    Bibersteinia-trehalosi    Aeromonas-schubertii    Bordetella-avium    Aeromonas-sobria    Bordetella-bronchiseptica    Aeromonas-trota    Bordetella-holmesii    Aeromonas-veronii    Bordetella-parapertussis    Afipia-broomeae    Bordetella-pertussis    Afipia-clevelandensis    Sherlock Instant FAME Libraries – 2009

Genus-species IBA1 IR2A1 ITSA1 Genus-species IBA1 IR2A1 ITSA1 Bordetella-trematum    Chryseomonas-luteola    Bradyrhizobium-japonicum    Citrobacter-amalonaticus    Brevibacillus-agri    Citrobacter-braakii    Brevibacillus-brevis    Citrobacter-farmeri    Brevibacillus-centrosporus    Citrobacter-freundii    Brevibacillus-choshinensis    Citrobacter-koseri    Brevibacillus-laterosporus    Citrobacter-murliniae    Brevibacillus-parabrevis    Citrobacter-werkmanii    Brevibacterium-casei    Citrobacter-youngae    Brevibacterium-epidermidis    Clavibacter-michiganensis    Brevibacterium-iodinum    Comamonas-terrigena    Brevibacterium-linens    Comamonas-testosteroni    Brevibacterium-stationis    Corynebacterium-afermentans    Brevundimonas-diminuta    Corynebacterium-amycolatum    Brevundimonas-vesicularis    Corynebacterium-auris    Brucella-melitensis    Corynebacterium-bovis    Budvicia-aquatica    Corynebacterium-callunae    Burkholderia-ambifaria    Corynebacterium-coyleae    Burkholderia-andropogonis    Corynebacterium-diphtheriae    Burkholderia-anthina    Corynebacterium-flavescens    Burkholderia-caryophylli    Corynebacterium-jeikeium    Burkholderia-cenocepacia    Corynebacterium-kutscheri    Burkholderia-cepacia    Corynebacterium-matruchotii    Burkholderia-cocovenenans    Corynebacterium-minutissimum    Burkholderia-gladioli    Corynebacterium-propinquum    Burkholderia-glathei    Corynebacterium-    Burkholderia-multivorans    pseudodiphtheriticum Burkholderia-pyrrocinia    Corynebacterium-    pseudotuberculosis Burkholderia-stabilis    Corynebacterium-renale    Buttiauxella-agrestis    Corynebacterium-seminale    Campylobacter-coli    Corynebacterium-striatum    Campylobacter-fetus    Corynebacterium-urealyticum    Campylobacter-hyointestinalis    Corynebacterium-xerosis    Campylobacter-jejuni    Cupriavidus-necator    Campylobacter-lari    Curtobacterium-flaccumfaciens    Campylobacter-sputorum    Curtobacterium-luteum    Cardiobacterium-hominis    Curtobacterium-pusillum    Cardiobacterium-valvarum    Cytophaga-allerginae"    Caulobacter-species    Deinococcus-radiodurans    Cedecea-davisae    Delftia-acidovorans    Cedecea-lapagei    Dermabacter-hominis    Cellulomonas-fimi    Dermacoccus-nishinomiyaensis    Cellulosimicrobium-cellulans    Dermatophilus-congolensis    Chitinophaga-arvensicola    Dietzia-maris    Chromobacterium-violaceum    Dysgonomonas-       Chryseobacterium-balustinum capnocytophagoides    Chryseobacterium-gleum Edwardsiella-hoshinae       Chryseobacterium-indologenes Edwardsiella-tarda       Chryseobacterium-indoltheticum Eikenella-corrodens    Sherlock Instant FAME Libraries – 2009

Genus-species IBA1 IR2A1 ITSA1 Genus-species IBA1 IR2A1 ITSA1 Elizabethkingia-meningoseptica    Gordonia-sputi    Empedobacter-brevis    Gordonia-terrae    Enterobacter-aerogenes    Grimontia-hollisae    Enterobacter-amnigenus    Haemophilus-influenzae    Enterobacter-asburiae    Haemophilus-parainfluenzae    Enterobacter-cancerogenus    Hafnia-alvei    Enterobacter-cloacae    Helicobacter-cinaedi    Enterobacter-gergoviae    Helicobacter-pylori    Enterobacter-hormaechei    Herbaspirillum-huttiense    Enterobacter-pyrinus    Hydrogenophaga-palleronii    Enterobacter-sakazakii    Hydrogenophaga-pseudoflava    Enterococcus-avium    Hydrogenophaga-taeniospiralis    Enterococcus-casseliflavus    Janthinobacterium-lividum    Enterococcus-cecorum    Jonesia-denitrificans    Enterococcus-columbae    Kingella-denitrificans    Enterococcus-dispar    Kingella-kingae    Enterococcus-durans    Klebsiella-oxytoca    Enterococcus-faecalis    Klebsiella-pneumoniae    Enterococcus-faecium    Kluyvera-ascorbata    Enterococcus-gallinarum    Kluyvera-intermedia    Enterococcus-hirae    Kocuria-kristinae    Enterococcus-malodoratus    Kocuria-rhizophila    Enterococcus-mundtii    Kocuria-rosea    Enterococcus-raffinosus    Kocuria-varians    Erysipelothrix-rhusiopathiae    Kytococcus-sedentarius    Escherichia-coli    Lactobacillus-delbrueckii    Escherichia-fergusonii    Lactobacillus-jensenii    Escherichia-hermannii    Lactobacillus-pentosus    Escherichia-vulneris    Lactobacillus-plantarum    Ewingella-americana    Lactobacillus-reuteri    Exiguobacterium-acetylicum    Lactobacillus-rhamnosus    Flavobacterium-aquatile    Lactobacillus-salivarius    Flavobacterium-johnsoniae    Lactococcus-garvieae    Flavobacterium-mizutaii    Lactococcus-lactis    Flavobacterium-resinovorum    Lactococcus-plantarum    Fluoribacter-bozemanae    Lechevalieria-aerocolonigenes    Fluoribacter-dumoffii    Lechevalieria-flava    Fluoribacter-gormanii    Leclercia-adecarboxylata    Francisella-philomiragia    Legionella-adelaidensis    Francisella-tularensis    Legionella-anisa    Gardnerella-vaginalis    Legionella-birminghamensis    Gemella-haemolysans    Legionella-brunensis    Gemella-morbillorum    Legionella-cherrii    Geobacillus-stearothermophilus    Legionella-cincinnatiensis    Gluconobacter-asaii    Legionella-fairfieldensis    Gordonia-aichiensis    Legionella-feeleii    Gordonia-amarae    Legionella-geestiana    Gordonia-bronchialis    Legionella-gratiana    Gordonia-rubripertincta    Legionella-jamestowniensis    Sherlock Instant FAME Libraries – 2009

Genus-species IBA1 IR2A1 ITSA1 Genus-species IBA1 IR2A1 ITSA1 Legionella-jordanis    Microbacterium-flavescens    Legionella-lansingensis    Microbacterium-hominis    Legionella-londiniensis    Microbacterium-    Legionella-longbeachae    hydrocarbonoxydans Legionella-nautarum    Microbacterium-imperiale    Legionella-oakridgensis    Microbacterium-keratanolyticum    Legionella-parisiensis    Microbacterium-lacticum    Legionella-pneumophila    Microbacterium-laevaniformans    Legionella-quateirensis    Microbacterium-liquefaciens    Legionella-quinlivanii    Microbacterium-luteolum    Legionella-rubrilucens    Microbacterium-maritypicum    Legionella-sainthelensi    Microbacterium-oxydans    Legionella-santicrucis    Microbacterium-paraoxydans    Legionella-shakespearei    Microbacterium-phyllosphaerae    Legionella-spiritensis    Microbacterium-resistens    Legionella-tucsonensis    Microbacterium-saperdae    Legionella-wadsworthii    Microbacterium-schleiferi    Legionella-worsleiensis    Microbacterium-species    Leifsonia-aquatica    Microbacterium-terrae    Leminorella-grimontii    Microbacterium-testaceum    Leminorella-richardii    Microbacterium-    trichothecenolyticum Leuconostoc-mesenteroides    Microbacterium-ulmi    Leuconostoc-    pseudomesenteroides Micrococcus-luteus    Listeria-grayi    Micrococcus-lylae    Listeria-innocua    Midi-calibration mix 1    Listeria-ivanovii    Moellerella-wisconsensis    Listeria-monocytogenes    Moraxella-atlantae    Listeria-seeligeri    Moraxella-bovis    Macrococcus-caseolyticus    Moraxella-canis    Marinibacillus-marinus    Moraxella-catarrhalis    Methylobacterium-aminovorans    Moraxella-lacunata    Methylobacterium-aquaticum    Moraxella-lincolnii    Methylobacterium-extorquens    Moraxella-nonliquefaciens    Methylobacterium-fujisawaense    Moraxella-osloensis    Methylobacterium-mesophilicum    Moraxella-ovis    Methylobacterium-organophilum    Morganella-morganii    Methylobacterium-radiotolerans    Mycobacterium-abscessus    Methylobacterium-rhodesianum    Mycobacterium-africanum    Methylobacterium-rhodinum    Mycobacterium-agri    Methylobacterium-zatmanii    Mycobacterium-aichiense    Microbacterium-aerolatum    Mycobacterium-asiaticum    Microbacterium-arborescens    Mycobacterium-aurum    Microbacterium-aurantiacum    Mycobacterium-brumae    Microbacterium-aurum    Mycobacterium-celatum    Microbacterium-barkeri    Mycobacterium-chelonae    Microbacterium-chocolatum    Mycobacterium-chitae    Microbacterium-dextranolyticum    Mycobacterium-    chlorophenolicum Microbacterium-esteraromaticum    Mycobacterium-chubuense    Sherlock Instant FAME Libraries – 2009

Genus-species IBA1 IR2A1 ITSA1 Genus-species IBA1 IR2A1 ITSA1 Mycobacterium-confluentis    Nocardia-seriolae    Mycobacterium-diernhoferi    Nocardia-transvalensis    Mycobacterium-fallax    Nocardioides-albus    Mycobacterium-flavescens    Nocardiopsis-dassonvillei    Mycobacterium-fortuitum    Nocardiopsis-prasina    Mycobacterium-gastri    Novosphingobium-    Mycobacterium-gordonae    aromaticivorans Mycobacterium-intermedium    Novosphingobium-subterraneum    Mycobacterium-intracellulare    Ochrobactrum-anthropi    Mycobacterium-kansasii    Oerskovia-turbata    Mycobacterium-komossense    Oligella-ureolytica    Mycobacterium-marinum    Oligella-urethralis    Mycobacterium-moriokaense    Paenibacillus-alvei    Mycobacterium-mucogenicum    Paenibacillus-amylolyticus    Mycobacterium-neoaurum    Paenibacillus-glucanolyticus    Mycobacterium-    Paenibacillus-lautus    nonchromogenicum Paenibacillus-macerans    Mycobacterium-obuense    Paenibacillus-pabuli    Mycobacterium-peregrinum    Paenibacillus-polymyxa    Mycobacterium-phlei    Paenibacillus-thiaminolyticus    Mycobacterium-scrofulaceum    Paenibacillus-validus    Mycobacterium-simiae    Pandoraea-apista    Mycobacterium-smegmatis    Pandoraea-pnomenusa    Mycobacterium-sphagni    Pandoraea-pulmonicola    Mycobacterium-szulgai    Pantoea-agglomerans    Mycobacterium-thermoresistibile    Pantoea-dispersa    Mycobacterium-triviale    Paracoccus-denitrificans    Mycobacterium-vaccae    Pasteurella-bettyae    Mycobacterium-valentiae"    Pasteurella-canis    Mycobacterium-xenopi    Pasteurella-multocida    Myroides-odoratus    Pectobacterium-carotovorum    Neisseria-cinerea    Pectobacterium-cypripedii    Neisseria-elongata    Pediococcus-acidilactici    Neisseria-flavescens    Pediococcus-dextrinicus    Neisseria-gonorrhoeae    Pediococcus-parvulus    Neisseria-meningitidis    Pediococcus-pentosaceus    Neisseria-mucosa    Photobacterium-damselae    Neisseria-sicca    Phyllobacterium-myrsinacearum    Neisseria-subflava    Phyllobacterium-rubiacearum    Neisseria-weaveri    Plesiomonas-shigelloides    Nesterenkonia-halobia    Pragia-fontium    Nocardia-asteroides    Proteus-hauseri    Nocardia-brasiliensis    Proteus-mirabilis    Nocardia-brevicatena    Proteus-penneri    Nocardia-farcinica    Proteus-vulgaris    Nocardia-globerula    Providencia-alcalifaciens    Nocardia-nova    Providencia-heimbachae    Nocardia-otitidiscaviarum    Providencia-rettgeri    Nocardia-pseudobrasiliensis    Providencia-rustigianii    Sherlock Instant FAME Libraries – 2009

Genus-species IBA1 IR2A1 ITSA1 Genus-species IBA1 IR2A1 ITSA1 Providencia-stuartii    Shigella-flexneri    Pseudoalteromonas-nigrifaciens    Shigella-sonnei    Pseudomonas-aeruginosa    Sphingobacterium-antarcticum    Pseudomonas-alcaligenes    Sphingobacterium-multivorum    Pseudomonas-balearica    Sphingobacterium-spiritivorum    Pseudomonas-fluorescens    Sphingobacterium-thalpophilum    Pseudomonas-mendocina    Sphingobium-chlorophenolicum    Pseudomonas-oryzihabitans    Sphingobium-herbicidovorans    Pseudomonas-pertucinogena    Sphingobium-yanoikuyae    Pseudomonas-    Sphingomonas-adhaesiva    pseudoalcaligenes Sphingomonas-capsulata    Pseudomonas-putida    Sphingomonas-echinoides    Pseudomonas-stutzeri    Sphingomonas-parapaucimobilis    Pseudomonas-syringae    Sphingomonas-paucimobilis    Psychrobacter-immobilis    Sphingomonas-sanguinis    Psychrobacter-phenylpyruvicus    Sphingomonas-species b    Ralstonia-pickettii    Sphingomonas-species f    Ralstonia-solanacearum    Sphingopyxis-macrogoltabida    Rhizobium-radiobacter    Sphingopyxis-terrae    Rhodobacter-sphaeroides    Staphylococcus-arlettae    Rhodococcus-coprophilus    Staphylococcus-aureus    Rhodococcus-equi    Staphylococcus-auricularis    Rhodococcus-erythropolis    Staphylococcus-capitis    Rhodococcus-fascians    Staphylococcus-caprae    Rhodococcus-globerulus    Staphylococcus-carnosus    Rhodococcus-rhodnii    Staphylococcus-chromogenes    Rhodococcus-rhodochrous    Staphylococcus-cohnii    Rhodococcus-wratislaviensis    Staphylococcus-delphini    Riemerella-anatipestifer    Staphylococcus-epidermidis    Roseomonas-cervicalis    Staphylococcus-equorum    Roseomonas-fauriae    Staphylococcus-felis    Roseomonas-genomospecies    Staphylococcus-gallinarum    Roseomonas-genomospecies 4    Staphylococcus-haemolyticus    Roseomonas-genomospecies 5    Staphylococcus-hominis    Roseomonas-genomospecies 6    Staphylococcus-hyicus    Roseomonas-gilardii    Staphylococcus-intermedius    Rothia-dentocariosa    Staphylococcus-kloosii    Rothia-mucilaginosa    Staphylococcus-lentus    Salmonella-bongori    Staphylococcus-lugdunensis    Salmonella-enterica    Staphylococcus-lutrae    Serratia-liquefaciens    Staphylococcus-muscae    Serratia-marcescens    Staphylococcus-pasteuri    Serratia-odorifera    Staphylococcus-piscifermentans    Serratia-plymuthica    Staphylococcus-pulvereri    Serratia-rubidaea    Staphylococcus-saccharolyticus    Shewanella-algae    Staphylococcus-saprophyticus    Shewanella-putrefaciens    Staphylococcus-schleiferi    Shigella-boydii    Staphylococcus-sciuri    Shigella-dysenteriae    Staphylococcus-simulans    Sherlock Instant FAME Libraries – 2009

Genus-species IBA1 IR2A1 ITSA1 Genus-species IBA1 IR2A1 ITSA1 Staphylococcus-vitulinus    Virgibacillus-halodenitrificans    Staphylococcus-warneri    Virgibacillus-pantothenticus    Staphylococcus-xylosus    Weeksella-virosa    Stenotrophomonas-maltophilia    Xanthobacter-agilis    Streptococcus-agalactiae    Xanthobacter-autotrophicus    Streptococcus-anginosus    Xanthobacter-flavus    Streptococcus-bovis    Xanthomonas-axonopodis    Streptococcus-canis    Yersinia-entercolitica    Streptococcus-cristatus    Yersinia-enterocolitica    Streptococcus-dysgalactiae    Yersinia-frederiksenii    Streptococcus-equi    Yersinia-intermedia    Streptococcus-equinus    Yersinia-kristensenii    Streptococcus-gallolyticus    Yersinia-pestis    Streptococcus-gordonii    Yersinia-pseudotuberculosis    Streptococcus-infantarius    Yersinia-ruckeri    Streptococcus-infantis    Yokenella-regensburgei    Streptococcus-iniae    Streptococcus-intermedius    Streptococcus-mitis    Streptococcus-mutans    Streptococcus-oralis    Streptococcus-parasanguinis    Streptococcus-peroris    Streptococcus-pneumoniae    Streptococcus-porcinus    Streptococcus-pyogenes    Streptococcus-salivarius    Streptococcus-sanguinis    Streptococcus-sobrinus    Streptococcus-species    Streptococcus-suis    Streptococcus-uberis    Streptococcus-vestibularis    Suttonella-indologenes    Tatlockia-maceachernii    Tatlockia-micdadei    Tatumella-ptyseos    Tetragenococcus-solitarius    Tetrasphaera-duodecadis    Trabulsiella-guamensis    Tsukamurella-inchonensis    Tsukamurella-paurometabola    Vibrio-alginolyticus    Vibrio-cholerae    Vibrio-fluvialis    Vibrio-mimicus    Vibrio-natriegens    Vibrio-parahaemolyticus    Vibrio-vulnificus    Sherlock Instant FAME Libraries – 2009

Instant Yeast Library (ITY1) Candida-albicans Candida-boidinii Candida-catenulata Candida-colliculosa Candida-famata Candida-glabrata Candida-guilliermondii Candida-haemulonii Candida-inconspicua Candida-intermedia Candida-kefyr Candida-krusei Candida-lambica Candida-lusitaniae Candida-magnoliae Candida-norvegensis Candida-parapsilosis Candida-rugosa Candida-sake Candida-sphaerica Candida-tropicalis Candida-utilis Candida-zeylanoides Cryptococcus-albidus Cryptococcus-laurentii Cryptococcus-neoforman Cryptococcus-terreus Kloeckera-apiculata Kluyveromyces-lactis Kodamaea-ohmeri Pichia-farinosa Prototheca-wickerhamii Rhodotorula-glutinis Rhodotorula-minuta Rhodotorula-mucilagino Saccharomyces-cerevisi Stephanoascus-ciferrii Trichosporon-inkin Trichosporon-mucoides Yarrowia-lipolytica Zygosaccharomyces-bail The information in this publication is subject to change without notice. Copyright  2009 MIDI, Inc. All Rights Reserved Date: 04 May 2009 MIDI, Inc. 125 Sandy Drive Newark, Delaware 19713 Phone: 302-737-4297 Fax: 302-737-7781 Email: [email protected] www.midi-inc.com