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Patient Sperm Cryopreservation Program

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Patient Sperm Cryopreservation Program Andrology Laboratory Center for Advanced Reproductive Care PATIENT SPERM CRYOPRESERVATION PROGRAM PATIENT SPERM BANK PROGRAM Semen Cryopreservation Conceiving with Cryopreserved Sperm Sperm cryopreservation allows patients to preserve or Intrauterine Insemination (IUI) Patients with normal, “bank” sperm prior to undergoing treatment for cancer, pre-cryopreservation, sperm parameters (20 million vasectomy, gender affirming treatments or face other motile sperm and >50% motility) may be able to procedures or situations that may lead to reduced conceive with intrauterine insemination. For intrauterine fertility or sterilization. insemination the cryopreserved sperm are thawed, washed, concentrated in a small volume and inserted Sperm Viability After Cryopreservation into the wife’s uterus on the day of expected ovulation. Approximately 50% of the motile sperm retain motility This procedure may be performed in a physician’s when thawed. Sperm count, percent motility, and office and is the least expensive medically assisted post-thaw viability will vary for each semen sample. reproduction technique and has a pregnancy rate of Poor quality specimens do not freeze as well as 15% per cycle. The optimal number of post-thaw motile normal semen samples. The number of motile sperm sperm for achieving a pregnancy with intrauterine pre-cryopreservation required for medically assisted insemination is 10 million/insemination. Patients may reproduction techniques ranges from less than wish to try this method first, while reserving enough 10,000 (in vitro fertilization with micromanipulation) frozen sperm for use in the in vitro fertilization program to 20 million (intrauterine insemination). We will if a pregnancy is not achieved through intrauterine thaw a small portion of the specimen shortly after insemination. Health insurance plans may cover some cryopreservation to determine the percentage of motile or none of these charges. sperm that are expected to survive cryopreservation. This information will be valuable to physicians should In Vitro Fertilization (IVF) Patients with less than you need a medically assisted reproduction procedure 20 million motile sperm cryopreserved are likely to in the future. require IVF and embryo transfer (IVF-ET). IVF facilitates the process of fertilization by inseminating eggs Morphological Evaluation of Sperm outside the body. Embryos resulting from this process Evaluation of sperm morphology is a method to are transferred to the uterus 5 days after the egg measure sperm quality. A small sample of the retrieval. Health insurance plans may cover some or specimen to be frozen is fixed onto a microscope none of these charges. slide and two hundred sperm are observed and rated according to a standard set of values for head For more information about the intrauterine shape, length, width and any other abnormalities that insemination or in vitro fertilization program please may be present. The ability of the sperm to fertilize visit our website: uihc.org/andrology-laboratory an egg in vitro is correlated with the percentage of morphologically normal sperm in the ejaculate. This or contact: information will be important for selecting a medically University of Iowa Health Care assisted reproduction technique for future pregnancy Center for Advanced Reproductive Care attempts. A morphology slide will be retained by 1360 N Dodge Street, Suite 2000 the Andrology Laboratory and will be evaluated upon Iowa City, IA 52245 physician request. 319-356-8483 for IVF or 319-356-1767 for IUI Directions from I-80 • Exit 246 • Turn South onto Dodge Street • Turn Right on N Dubuque Rd • Facility will be on the right. Andrology Laboratory Center for Advanced Reproductive Care PATIENT SPERM CRYOPRESERVATION PROGRAM SCHEDULING, PRE-BANKING REQUIREMENTS, SAMPLE COLLECTION, CRYOPRESERVATION, AND STORAGE PROCEDURES Scheduling cryopreservation and the specimen is transferred You may schedule an appointment at the UI Health Care to cryovials. The average ejaculate will fill 1-3 vials. Andrology Laboratory for sperm cryopreservation by The sperm are then frozen and stored in a liquid calling 319-467-5299. Appointment times are Monday- nitrogen tank at -196ºC. Sperm may be stored at this Friday, between 8 a.m.- 2 p.m. A physician requisition is temperature indefinitely. required for all testing. Storage Before you store a semen sample you need to be tested The UI Health Care Andrology Laboratory is a member for the following infectious diseases: of the ReproTech, Ltd. Fertility Preservation Network. • HIV I/II Antibody (or HIV Antigen/Antibody Combo) The network is based on ReproTech’s long history in • Hepatitis B Surface Antigen sperm banking and their leadership and expertise in long term storage. The cryopreserved sperm will be • Hepatitic C Antibody sent to ReproTech for long-term sperm storage. For If testing is done outside of University of Iowa Hospitals more information about ReproTech please visit their & Clinics, please have your referring physician’s office website: http://www.reprotech.com/ send us the test results. Results Two to seven days of abstinence from ejaculation is Results may be viewed by patients via MyChart within recommended prior to the day of specimen collection 24 hours of testing. A final copy of the results will to optimize the number of motile sperm in the ejaculate. be mailed within seven business days to ordering You should schedule your appointments accordingly. physicians outside UI Health Care. The Andrology Laboratory is located at Fees • UI Health Care North Dodge Semen Freeze $331.00 1360 North Dodge Street, Level 2 (Includes $174.00 charge for Seminal Fluid Analysis) Specimen Collection Sperm Morphology $169.00 (only if requested by physician) The specimen will be collected in a private room. In order to keep the specimen as sterile as possible, For scheduling or further information about the UI it must be collected into a sterile container by Health Care Andrology Laboratory, please contact us: masturbation. The laboratory will provide a sterile, non- University of Iowa Health Care North Dodge spermicidal lubricant if you require a lubricant. Attn: Andrology Laboratory You will be asked to sign consent forms to ship and 1360 N Dodge Street, Suite 2000 store your cryopreserved sperm at the ReproTech Iowa City, IA 52245-6104 storage facility. PHONE: 319-467-5299 Cryopreservation FAX: 319-384-8353 The semen analysis will be completed approximately one hour after the specimen arrives at the laboratory. We evaluate the ejaculate volume, seminal fluid consistency, sperm count, and sperm motility. Following the analysis, the semen is diluted with a cryoprotectant to protect the sperm during .
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