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Diapositiva 1 Figura Suplementaria S20. Dendrograma de MALDI-TOF MS obtenido a partir de todos los espectros proteicos de los aislados pertenecientes a la familia Staphylococcaceae. En el dendrograma se incluyen todas las cepas de la base de datos del programa Biotyper (indicadas en negro) y las cepas del estudio previo realizado en el Hospital Son Llàtzer (indicadas en azul), pertenecientes a esta familia. En rojo se marcan los aislados de los cuales se dispone de secuencia del gen 16S rRNA y su identificación presuntiva. A la derecha de la figura se ha incluido un resumen del origen y el número de aislados para cada muestreo analizados, en rojo (Nov-12), azul (Feb-13), verde (May-13), naranja (Ago-13) y gris (Nov-13). Los cuadros sin relleno muestran el número de aislados secuenciados de cada muestreo. Staphylococcus sp[1] 4 PIM Staphylococcus sp[1] CS 331_1 BRB Staphylococcus hominis 18 ESL Staphylococcus hominis Mb18788_1 CHB Staphylococcus hominis ssp novobiosepticus DSM 15614ᵀ Staphylococcus hominis ssp hominis DSM 20328ᵀ Staphylococcus hominis ssp hominis DSM 20329 Staphylococcus hominis ssp hominis DSM 20330 Staphylococcus haemolyticus Mb18803_2 CHB Staphylococcus haemolyticus DSM 20265 Staphylococcus haemolyticus 19 ESL Staphylococcus haemolyticus DSM 20264 Staphylococcus haemolyticus 10024 CHB Staphylococcus haemolyticus DSM 20228 Staphylococcus haemolyticus DSM 20263ᵀ Staphylococcus sp[2] CS 546_1 BRB Staphylococcus simulans DSM 20322ᵀ Staphylococcus simulans DSM 20323 Staphylococcus simulans DSM 20324 Staphylococcus carnosus ssp carnosus DSM 20501ᵀ Staphylococcus condimenti DSM 11674ᵀ Staphylococcus condimenti DSM 11675 Staphylococcus piscifermentans DSM 7373ᵀ Staphylococcus carnosus ssp utilis DSM 11676ᵀ Staphylococcus carnosus ssp utilis DSM 11677 Staphylococcus simulans DSM 20723 Staphylococcus kloosii DSM 20677 Dᵀ Staphylococcus kloosii DSM 20676ᵀ Staphylococcus kloosii DSM 20677 Staphylococcus arlettae DSM 20672ᵀ Staphylococcus arlettae DSM 20673 Staphylococcus arlettae CS 678_7 BRB Staphylococcus succinus ssp casei DSM 15096ᵀ Staphylococcus succinus ssp succinus DSM 14617ᵀ Staphylococcus xylosus DSM 20266ᵀ Staphylococcus xylosus DSM 20267 Staphylococcus xylosus DSM 6179 Staphylococcus xylosus FI FLR Staphylococcus saprophyticus ssp saprophyticus DSM 20038 Staphylococcus saprophyticus ssp saprophyticus DSM 20229ᵀ Staphylococcus cohnii ssp cohnii DSM 20260ᵀ Staphylococcus cohnii ssp cohnii DSM 20261 Staphylococcus cohnii ssp cohnii DSM 20262 Staphylococcus cohnii ssp urealyticus DSM 6718ᵀ Staphylococcus cohnii ssp urealyticus DSM 6719 Staphylococcus nepalensis DSM 15150T Staphylococcus nepalensis DSM 15151 Staphylococcus saprophyticus ssp bovis DSM 18669ᵀ Staphylococcus saprophyticus ssp saprophyticus CCM 2354 Staphylococcus saprophyticus ssp saprophyticus CCM 3318 Staphylococcus saprophyticus ssp saprophyticus CCM 2602 Staphylococcus saprophyticus ssp saprophyticus CCM 3319 Staphylococcus equorum ssp equorum DSM 20674ᵀ Staphylococcus equorum ssp equorum DSM 20675 Staphylococcus equorum V794 MCRF Staphylococcus schleiferi ssp schleiferi DSM 4809 Staphylococcus schleiferi ssp schleiferi DSM 4807ᵀ Staphylococcus schleiferi ssp schleiferi DSM 4808 Staphylococcus schleiferi RV_BA_03_C LBK Staphylococcus schleiferi ssp coagulans 0903M10_070501 IBS Staphylococcus chromogenes DSM 20454ᵀ Staphylococcus hyicus DSM 20459ᵀ Staphylococcus hyicus DSM 17421 Staphylococcus lutrae DSM 10246 Staphylococcus lutrae DSM 10244ᵀ Staphylococcus lutrae DSM 10245 Staphylococcus felis DSM 7377ᵀ Staphylococcus felis V248 MCRF Staphylococcus felis V351 MCRF Staphylococcus felis V461 MCRF Staphylococcus felis V766 MCRF Staphylococcus auricularis DSM 20609 Staphylococcus muscae DSM 7068ᵀ Staphylococcus intermedius DSM 203731ᵀ Staphylococcus intermedius DSM 20373ᵀ Staphylococcus delphini DSM 20771ᵀ Staphylococcus intermedius 08_STAINT MVO Staphylococcus pseudintermedius DSM 21284ᵀ Staphylococcus pseudintermedius CCUG 32915 Staphylococcus sciuri ssp sciuri DSM 20345ᵀ Staphylococcus sciuri ssp carnaticus DSM 15613ᵀ Staphylococcus sciuri ssp sciuri DSM 6671 Staphylococcus lentus DSM 20352ᵀ Staphylococcus lentus DSM 6672 Staphylococcus fleurettii DSM 13212ᵀ Staphylococcus sciuri ssp rodentium DSM 16827ᵀ Staphylococcus vitulinus DSM 15615ᵀ Staphylococcus vitulinus DSM 9931 Staphylococcus vitulinus DSM 9930 Staphylococcus epidermidis MG90 2 0 0 0 0 No12_91 Staphylococcus epidermidis ATCC 14990ᵀ No12_94 2 0 0 0 0 Ago13_70 Ago13_74 No12_102 No12_123 No12_39 No12_40 No12_41 6 1 1 2 1 Staphylococcus pasteuri DSM 10656ᵀ Staphylococcus warneri ATCC 27836ᵀ Staphylococcus pasteuri DSM 10657 5 1 1 2 1 No12_103 Staphylococcus warneri DSM 20036 Staphylococcus warneri DSM 20316ᵀ Staphylococcus warneri Mb18796_1 CHB May13_101 Feb13_5 No13_171 Staphylococcus caprae DSM 20608ᵀ No13_187 No13_184 No13_188 Staphylococcus epidermidis 4b_r ESL Staphylococcus epidermidis 6b_s ESL No13_176 Staphylococcus epidermidis ATCC 12228 Staphylococcus epidermidis ATCC 12228 Staphylococcus epidermidis ATCC 14990ᵀ May13_17 May13_184 Staphylococcus saprophyticus ATCC15305ᵀ Staphylococcus epidermidis DSM 3269 Staphylococcus epidermidis DSM 1798 0 0 1 0 17 No13_190 Staphylococcus epidermidis ATCC 14990ᵀ No13_189 0 0 1 0 7 No13_177 No13_181 No13_183 No13_179 No13_180 No13_182 Staphylococcus epidermidis 10547 CHB No13_178 No13_185 No13_186 No13_175 No13_191 Staphylococcus pettenkoferi DSM 19554ᵀ Staphylococcus pettenkoferi KL906013_BK14723 DT Staphylococcus pettenkoferi 0903M12_075701 IBS Staphylococcus pettenkoferi KL906013_BK14723 Staphylococcus capitis ssp urealyticus DSM 6717ᵀ Staphylococcus capitis Mb18717_1 CHB Staphylococcus capitis ssp capitis DSM 20325 Staphylococcus capitis ssp capitis DSM 20326ᵀ Staphylococcus capitis ssp capitis DSM 6180 1 0 0 0 0 No12_104 Staphylococcus capitis subs capitis ATCC27840ᵀ Staphylococcus lugdunensis DSM 4804ᵀ 1 0 0 0 0 Staphylococcus lugdunensis DSM 4806 Staphylococcus lugdunensis DSM 4805 Staphylococcus lugdunensis 20659_1 CHB Staphylococcus lugdunensis DSM 6670 Staphylococcus aureus ssp aureus DSM 11822 Staphylococcus aureus ssp aureus DSM 20491 Staphylococcus aureus ssp aureus DSM 3463 Staphylococcus aureus ssp aureus DSM 20232 Staphylococcus aureus ATCC 33591 Staphylococcus aureus ATCC 33862 Staphylococcus aureus ATCC 25923 Staphylococcus aureus ATCC 29213 Staphylococcus aureus ssp aureus DSM 346 Staphylococcus aureus ssp aureus DSM 4910 Staphylococcus aureus ssp anaerobius DSM 20714ᵀ Staphylococcus aureus ssp aureus DSM 20652 Staphylococcus simiae DSM 17638 Staphylococcus simiae DSM 17639 Staphylococcus simiae DSM 17636ᵀ Staphylococcus simiae DSM 17637 1000 800 600 400 200 0 .
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