biomolecules Review Interfaces with Structure Dynamics of the Workhorses from Cells Revealed through Cross-Linking Mass Spectrometry (CLMS) Umesh Kalathiya 1,*,† , Monikaben Padariya 1,†, Jakub Faktor 1 , Etienne Coyaud 2, Javier A. Alfaro 1,3, Robin Fahraeus 1, Ted R. Hupp 1,3 and David R. Goodlett 1,4,5,* 1 International Centre for Cancer Vaccine Science, University of Gdansk, ul. Kładki 24, 80-822 Gdansk, Poland;
[email protected] (M.P.);
[email protected] (J.F.);
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[email protected] (T.R.H.) 2 Protéomique Réponse Inflammatoire Spectrométrie de Mass—PRISM, Inserm U1192, University Lille, CHU Lille, F-59000 Lille, France;
[email protected] 3 Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, Scotland EH4 2XR, UK 4 Department of Biochemistry & Microbiology, University of Victoria, Victoria, BC V8Z 7X8, Canada 5 Genome BC Proteome Centre, University of Victoria, Victoria, BC V8Z 5N3, Canada * Correspondence:
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[email protected] (D.R.G.) † These authors contributed equally to this work. Abstract: The fundamentals of how protein–protein/RNA/DNA interactions influence the structures and functions of the workhorses from the cells have been well documented in the 20th century. A di- Citation: Kalathiya, U.; Padariya, M.; verse set of methods exist to determine such interactions between different components, particularly, Faktor, J.; Coyaud, E.; Alfaro, J.A.; the mass spectrometry (MS) methods, with its advanced instrumentation, has become a significant Fahraeus, R.; Hupp, T.R.; Goodlett, approach to analyze a diverse range of biomolecules, as well as bring insights to their biomolecular D.R.