FAK / Focal Adhesion Kinase Antibody (C-Terminus) Rabbit Polyclonal Antibody Catalog # ALS10614

10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609

Specification

FAK / Focal Adhesion Kinase Antibody (C-Terminus) - Product Information

Application IHC Primary Accession Q05397 Reactivity Human, Mouse, Monkey Host Rabbit Clonality Polyclonal Calculated MW 119kDa KDa

FAK / Focal Adhesion Kinase Antibody (C-Terminus) - Additional Information

Gene ID 5747 Anti-FAK / Focal Adhesion Kinase antibody IHC of human Brain, Glioblastoma. Other Names Focal adhesion kinase 1, FADK 1, 2.7.10.2, Focal adhesion kinase-related nonkinase, FRNK, Protein phosphatase 1 regulatory subunit 71, PPP1R71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, PTK2, FAK, FAK1

Target/Specificity Human FAK. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Reconstitution & Storage Long term: -70°C; Short term: +4°C Anti-FAK antibody ALS10614 IHC of human Precautions lobular epithelium. FAK / Focal Adhesion Kinase Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.

FAK / Focal Adhesion Kinase Antibody (C-Terminus) - Protein Information

Name PTK2 (HGNC:9611)

Synonyms FAK, FAK1

Function

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Non-receptor protein-tyrosine kinase that Anti-FAK / Focal Adhesion Kinase antibody plays an essential role in regulating cell IHC of human Skin, Melanoma. migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal FAK / Focal Adhesion Kinase Antibody adhesions and cell protrusions, cell cycle (C-Terminus) - Background progression, cell proliferation and apoptosis. Required for early embryonic Non-receptor protein-tyrosine kinase that development and placenta development. plays an essential role in regulating cell Required for embryonic angiogenesis, normal cardiomyocyte migration and migration, adhesion, spreading, reorganization proliferation, and normal heart of the actin cytoskeleton, formation and development. Regulates axon growth and disassembly of focal adhesions and cell neuronal cell migration, axon branching and protrusions, cell cycle progression, cell synapse formation; required for normal proliferation and apoptosis. Required for early development of the nervous system. Plays a embryonic development and placenta role in osteogenesis and differentiation of development. Required for embryonic osteoblasts. Functions in integrin signal angiogenesis, normal cardiomyocyte migration transduction, but also in signaling and proliferation, and normal heart downstream of numerous growth factor development. Regulates axon growth and receptors, G-protein coupled receptors neuronal cell migration, axon branching and (GPCR), EPHA2, netrin receptors and LDL synapse formation; required for normal receptors. Forms multisubunit signaling development of the nervous system. Plays a complexes with SRC and SRC family role in osteogenesis and differentiation of members upon activation; this leads to the osteoblasts. Functions in integrin signal phosphorylation of additional tyrosine transduction, but also in signaling downstream residues, creating binding sites for scaffold of numerous growth factor receptors, G-protein proteins, effectors and substrates. coupled receptors (GPCR), EPHA2, netrin Regulates numerous signaling pathways. receptors and LDL receptors. Forms Promotes activation of phosphatidylinositol multisubunit signaling complexes with SRC and 3-kinase and the AKT1 signaling cascade. SRC family members upon activation; this Promotes activation of MAPK1/ERK2, leads to the phosphorylation of additional MAPK3/ERK1 and the MAP kinase signaling tyrosine residues, creating binding sites for cascade. Promotes localized and transient scaffold proteins, effectors and substrates. activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating Regulates numerous signaling pathways. proteins (GAPs), and thereby modulates the Promotes activation of phosphatidylinositol activity of Rho family GTPases. Signaling via 3-kinase and the AKT1 signaling cascade. CAS family members mediates activation of Promotes activation of MAPK1/ERK2, RAC1. Recruits the ubiquitin ligase MDM2 to MAPK3/ERK1 and the MAP kinase signaling P53/TP53 in the nucleus, and thereby cascade. Promotes localized and transient regulates P53/TP53 activity, P53/TP53 activation of guanine nucleotide exchange ubiquitination and proteasomal factors (GEFs) and GTPase-activating proteins degradation. Phosphorylates SRC; this (GAPs), and thereby modulates the activity of increases SRC kinase activity. Rho family GTPases. Signaling via CAS family Phosphorylates ACTN1, ARHGEF7, GRB7, members mediates activation of RAC1. RET and WASL. Promotes phosphorylation Recruits the ubiquitin ligase MDM2 to P53/TP53 of PXN and STAT1; most likely PXN and in the nucleus, and thereby regulates P53/TP53 STAT1 are phosphorylated by a SRC family activity, P53/TP53 ubiquitination and kinase that is recruited to proteasomal degradation. Phosphorylates SRC; autophosphorylated PTK2/FAK1, rather than this increases SRC kinase activity. by PTK2/FAK1 itself. Promotes Phosphorylates ACTN1, ARHGEF7, GRB7, RET phosphorylation of BCAR1; GIT2 and SHC1; and WASL. Promotes phosphorylation of PXN this requires both SRC and PTK2/FAK1. and STAT1; most likely PXN and STAT1 are Promotes phosphorylation of BMX and phosphorylated by a SRC family kinase that is PIK3R1. Isoform 6 (FRNK) does not contain a recruited to autophosphorylated PTK2/FAK1, kinase domain and inhibits PTK2/FAK1 rather than by PTK2/FAK1 itself. Promotes

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phosphorylation and signaling. Its enhanced phosphorylation of BCAR1; GIT2 and SHC1; this expression can attenuate the nuclear requires both SRC and PTK2/FAK1. Promotes accumulation of LPXN and limit its ability to phosphorylation of BMX and PIK3R1. Isoform 6 enhance serum response factor (FRNK) does not contain a kinase domain and (SRF)-dependent gene transcription. inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can Cellular Location attenuate the nuclear accumulation of LPXN Cell junction, focal adhesion. Cell and limit its ability to enhance serum response membrane; Peripheral membrane protein; factor (SRF)-dependent gene transcription. Cytoplasmic side. Cytoplasm, cell cortex Cytoplasm, cytoskeleton. Cytoplasm, FAK / Focal Adhesion Kinase Antibody cytoskeleton, microtubule organizing (C-Terminus) - References center, centrosome. Nucleus. Cytoplasm, cytoskeleton, cilium basal body Whitney G.S.,et al.DNA Cell Biol. Note=Constituent of focal adhesions. 12:823-830(1993). Detected at microtubules Andre E.,et al.Biochem. Biophys. Res. Tissue Location Commun. 190:140-147(1993). Detected in B and T-lymphocytes. Isoform 1 Ota T.,et al.Nat. Genet. 36:40-45(2004). and isoform 6 are detected in lung Nusbaum C.,et al.Nature 439:331-335(2006). fibroblasts (at protein level) Ubiquitous. Bienvenut W.V.,et al.Submitted (JUL-2007) to Expressed in epithelial cells (at protein UniProtKB. level) (PubMed:31630787).

Volume 50 µl

FAK / Focal Adhesion Kinase Antibody (C-Terminus) - Protocols

Provided below are standard protocols that you may find useful for product applications.

• Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture

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