Experiment 4 LABORATORY TECHNIQUES Chem 110 Lab
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AOAC Official Method 2011.25 Insoluble, Soluble, and Total Dietary Fiber in Foods
AOAC Official Method 2011.25 supplies/fisherbrand.html) or 250 mL polypropylene bottles with Insoluble, Soluble, and Total Dietary Fiber in Foods polypropylene caps. Enzymatic-Gravimetric-Liquid Chromatography (c) Fritted crucible.—Gooch, fritted disk, Pyrex® 50 mL, First Action 2011 pore size, coarse, ASTM 40–60 µm (Corning No. 32940-50C® [Applicable to plant material, foods, and food ingredients or equivalent; http://www.labplanet.com/corning-crucible-gooch- consistent with CODEX Alimentarius Commission high-c-50-ml-32940-50c.html). Prepare four for each sample as Definition adopted in 2009 and modified slightly in 2010 follows: Ash overnight at 525°C in muffle furnace. Cool furnace to (ALINORM 09/32/REP and ALINORM 10/33/REP, respectively), 130°C before removing crucibles to minimize breakage. Remove including naturally occurring, isolated, modified, and synthetic any residual Celite and ash material by using a vacuum. Soak in 2% polymers meeting that definition.] cleaning solution, C(i), at room temperature for 1 h. Rinse crucibles See Tables 2011.25A–H for the results of the interlaboratory with water and deionized water. For final rinse, use 15 mL acetone study supporting acceptance of the method. and air dry. Add approximately 1.0 g Celite to dried crucibles and A. Principle dry at 130°C to constant weight. Cool crucible in desiccators for approximately 1 h and record mass of crucible containing Celite. A method is described for the measurement of insoluble, (d) Filtering flask.—Heavy-walled, 1 L with side arm. soluble, and total dietary fiber (IDF, SDF, and TDF, respectively), (e) Rubber ring adaptors.—For use to join crucibles with inclusive of the resistant starch (RS) and the water:alcohol soluble filtering flasks. -
Zinc and Cadmium in Paper (Reaffirmation of T 438 Cm-96)
WI 050114.01 T 438 DRAFT NO. 5 DATE July 27, 2006 TAPPI WORKING GROUP CHAIRMAN J Ishley SUBJECT CATEGORY Fillers & Pigments Testing RELATED METHODS See “Additional Information” CAUTION: This Test Method may include safety precautions which are believed to be appropriate at the time of publication of the method. The intent of these is to alert the user of the method to safety issues related to such use. The user is responsible for determining that the safety precautions are complete and are appropriate to their use of the method, and for ensuring that suitable safety practices have not changed since publication of the method. This method may require the use, disposal, or both, of chemicals which may present serious health hazards to humans. Procedures for the handling of such substances are set forth on Material Safety Data Sheets which must be developed by all manufacturers and importers of potentially hazardous chemicals and maintained by all distributors of potentially hazardous chemicals. Prior to the use of this method, the user must determine whether any of the chemicals to be used or disposed of are potentially hazardous and, if so, must follow strictly the procedures specified by both the manufacturer, as well as local, state, and federal authorities for safe use and disposal of these chemicals. Zinc and cadmium in paper (Reaffirmation of T 438 cm-96) (no changes were made since last draft) 1. Scope and significance 1.1 This method maybe used for the determination of cadmium and zinc either in paper or in highly opaque pigments. Zinc is usually present in zinc oxide, zinc sulfide, or as lithopone (a combination of zinc sulfide and barium sulfate), which is occasionally used in filled paper, in paper coatings and in high-pressure laminates and wallpaper. -
Detection of Acid-Producing Bacteria Nachweis Von Säureproduzierenden Bakterien Détection De Bactéries Produisant Des Acides
(19) TZZ ¥ _T (11) EP 2 443 249 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.: of the grant of the patent: C12Q 1/04 (2006.01) G01N 33/84 (2006.01) 19.11.2014 Bulletin 2014/47 (86) International application number: (21) Application number: 10790013.6 PCT/US2010/038569 (22) Date of filing: 15.06.2010 (87) International publication number: WO 2010/147918 (23.12.2010 Gazette 2010/51) (54) DETECTION OF ACID-PRODUCING BACTERIA NACHWEIS VON SÄUREPRODUZIERENDEN BAKTERIEN DÉTECTION DE BACTÉRIES PRODUISANT DES ACIDES (84) Designated Contracting States: (74) Representative: Isarpatent AL AT BE BG CH CY CZ DE DK EE ES FI FR GB Patent- und Rechtsanwälte GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO Friedrichstrasse 31 PL PT RO SE SI SK SM TR 80801 München (DE) (30) Priority: 15.06.2009 US 187107 P (56) References cited: 15.03.2010 US 314140 P US-A- 4 528 269 US-A- 5 098 832 US-A- 5 164 301 US-A- 5 601 998 (43) Date of publication of application: US-A- 5 601 998 US-A- 5 786 167 25.04.2012 Bulletin 2012/17 US-B2- 6 756 225 US-B2- 7 150 977 (73) Proprietor: 3M Innovative Properties Company • DARUKARADHYA J ET AL: "Selective Saint Paul, MN 55133-3427 (US) enumeration of Lactobacillus acidophilus, Bifidobacterium spp., starter lactic acid bacteria (72) Inventors: and non-starter lactic acid bacteria from Cheddar • YOUNG, Robert, F. cheese", INTERNATIONAL DAIRY JOURNAL, Saint Paul, Minnesota 55133-3427 (US) ELSEVIER APPLIED SCIENCE, BARKING, GB, • MACH, Patrick, A. -
Gravimetric Analysis of Chloride
Chemistry 321L Manual Page 16 Gravimetric Analysis of Chloride I. Introduction The amount of chloride ion in a substance can be determined by precipitating the soluble chloride ion present with silver ion. The amount of chloride ion present will be related to the mass of silver chloride formed. + - Ag (aq) + Cl (aq) 6 AgCl (s) II. Procedure A. Preparation of Filter Crucibles (Sintered-Glass) 1. Use four filter crucibles of medium (M) porosity. Do not use crucibles marked C for coarse porosity. 2. Previously, the crucibles have been used to filter silver chloride (purple or gray, ammonia soluble). First, remove any bulk precipitate with a rubber policeman and dispose of it in a "Silver Collection" jar. Follow this with chemical cleaning. To remove silver chloride, invert the crucibles in a large beaker in a fume hood and add one or two mL of concentrated ammonia to each. Allow to soak until clean. Carefully remove the crucibles from the beaker and soak in water before removing from the fume hood. Set up a suction filtration apparatus and wash the crucible several times with water and finally with deionized water. Empty the suction flask before proceeding. Stains that are not removed by this cleaning process should not interfere with your measurements. Number, in pencil, each crucible on the frosted glass area. 3. Dry the crucibles in an oven at 120EC for about 1 hour, using a beaker, glass hooks, and watch glass (see figure). A ribbed watch glass can be used in place of the glass hooks. 4. Remove the dried crucibles from the oven and let them cool for about one minute on the counter top. -
Performance Validation of the Microbiologique Microfilm Test
MAI ET AL.: JOURNAL OF AOAC INTERNATIONAL VOL. 101, NO. X, 2018 1 FOOD BIOLOGICAL CONTAMINANTS Performance Validation of the Microbiologique MicrofilmTM Test System for AOAC Research Institute Performance Tested Method SMCertification AOAC Performance Tested Method SM 051702 Abstract ANNA SHAPOVALOVA and HARISH K. JANAGAMA Molecular Epidemiology, Inc., 15300 Bothell Way NE, The Microfilm™ Test System is intended for quantitative Lake Forest Park, WA 98155 microbiology and consists of three types of Microfilms for aerobic LONG VUONG and ALEX FRIEDRICH plate count (Microfilm APC), total coliform and Escherichia coli IEH Laboratories and Consulting Group, Inc., 15300 Bothell count (Microfilm TCEc), and yeast and mold count (Microfilm Way NE, Lake Forest Park, WA 98155 YMC). This study evaluated the performance of the Microfilm DYLAN JOHNSON Test System against International Organization for Standardization Molecular Epidemiology, Inc., 15300 Bothell Way NE, (ISO) methods on 20 food matrixes and 2 environmental surfaces. Lake Forest Park, WA 98155 Ruggedness, robustness, and stability were also determined, LOURDES M. NADALA while inclusivity and exclusivity studies were performed on Molecular Epidemiology, Inc., 15300 Bothell Way N.E., Microfilm TCEc and YMC. An independent laboratory evaluated Lake Forest Park, WA 98155; Microbiologique, the performance on four food matrixes and one environmental Inc., 8215 Lake City Way NE, Seattle, WA 98115 surface. No significant differences and high correlation coefficients VAN NGUYEN were observed between the Microfilm Test System and the Microbiologique, Inc., 8215 Lake City Way NE, Seattle, corresponding ISO methods (ISO 4833-1:2013 for APC, ISO WA 98115 4832:2006 for total coliform count, ISO 16649-2: 2001 for E. -
Funnels and Filters
51 FUNNELS AND FILTERS Buchner Funnels 260MM (10.25") INSIDE DIAMETER, 203MM (8") OVERALL HEIGHT, 127MM (5") RIM TO PLATE CATALOG NO. DESCRIPTION PRICE/EACH H14620-0000 Funnel with Coarse Porosity Fixed Plate $216.00 H14620-1260 Funnel with Coarse Porosity Removable Plate 216.00 H14625-3510 Funnel with Medium Porosity Fixed Plate 216.00 H14626-3510 Funnel with Medium Porosity Removable Plate 216.00 H14627-0000 Funnel with Perforated Fixed Plate 216.00 Table-Top H14627-1260 Funnel with Perforated Removable Plate 216.00 Buchner 457MM (18") INSIDE DIAMETER, 292MM (11.5") OVERALL HEIGHT, 203MM (8") RIM TO PLATE Funnels CATALOG NO. DESCRIPTION PRICE/EACH With Fritware® Porous H14621-0000 Funnel with Coarse Porosity Fixed Plate $540.00 Filter or HDPE H14621-1457 Funnel with Coarse Porosity Removable Plate 540.00 H14625-3518 Funnel with Medium Porosity Fixed Plate 540.00 Perforated Plate H14626-3518 Funnel with Medium Porosity Removable Plate 540.00 Polyethylene funnels are H14628-0000 Funnel with Perforated Fixed Plate 465.00 one piece design with a H14627-1457 Funnel with Perforated Removable Plate 465.00 FIXED or REMOVABLE Custom sizes available upon request. 610MM (24") INSIDE DIAMETER, 330MM (13") OVERALL HEIGHT, 267MM (10.25") RIM TO PLATE porous filter plate CATALOG NO. DESCRIPTION PRICE/EACH supported by a multiple ring grid. Filter plates are made of high density H14622-0000 Funnel with Coarse Porosity Fixed Plate $1125.00 1 polyethylene 6.4mm ( ⁄4") thick with a non-porous ring at the periphery of the plate H14622-1610 Funnel with Coarse Porosity Removable Plate 1125.00 1 H14625-3524 Funnel with Medium Porosity Fixed Plate 1125.00 which seals filter paper when used. -
ARC Laboratory Manual for Architectural Conservators
A LABORATORY MANUAL FOR ARCHITECTURAL CONSERVATORS Jeanne Marie Teutonico ICCROM Rome 1988 CONTENTS Foreword v Preface vii General Principles: Laboratory Science 1. Sampling 3 2. Measurement and Error: Precision, Accuracy, Statistics 7 3. Measurement: Mass (Use of the Balance) 11 4. Measurement: Length (Use of the Vernier Caliper and Micrometer) 16 5. Measurement: Volume 21 6. Measurement: Solutions 26 7. Measurement: pH 30 Porous Building Materials 8. Water Absorption by Total Immersion 35 9. Water Drop Absorption 41 10. Penetration of Water: Capillary Action 43 11. Porosity of Granular Beds 45 12. Porosity in Solids: Indirect Measurement by Water Absorption 50 13. Porosity in Solids: Hydrostatic Weighing 52 14. Movement of Salts 56 15. Salt Crystallization 57 16. Qualitative Analysis of Water-soluble Salts and This publication was printed with a generous Carbonates 58 contribution from the government of 17. Semiquantititive Analysis of Water-soluble Salts 68 Finland. Earthen Building Materials 18A. Particle Size Analysis: Part I Sieving Procedure 73 ISBN 92-9077-083-X Via di San Michele 13 00153 Rome RM, Italy Printed in Italy III Earthen Building Materials (continued) FOREWORD 18B. Particle Size Analysis: Part II Sedimentation Procedure: Hydrometer Method 83 19. Plastic Limit of Soils 96 After his appointment as director of ICCROM in 1977, Sir Bernard FEILDEN proposed to emphasize the interdisciplinary 20. Liquid Limit of Soils 102 character of conservation by introducing scientists to field work and architects to laboratories. Consequently it was decided to introduce a series of laboratory exercises Stone. Brick and Mortars designed for the needs of architectural conservators and conservation architects in the program of the International 21. -
Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position. -
Thermal Activity of Base Course Material Related to Pavement Cracking
THERMAL ACTIVITY OF BASE COURSE MATERIAL RELATED TO PAVEMENT CRACKING by Samuel H. Carpenter Robert L. Lytton Research Report Number 18-2 Environmental Deterioration of Pavement Research Project 2-8-73-18 Conducted for the State Department of Highways and Public Transportation in cooperation with the U.S. Department of Transportation Federal Highway Administration by the Texas Transportation Institute Texas A&M Univeristy College station, Texas December, 197 5 TECHNICAL REPORT STANDARD TITLE PACf 1. Report No. 2. Government Accession No. 3. Recipient's Catalog No. ~4.-;Ti~tl-e-an~d~Su~b~ti~tle------------~------------------------~S~.~R~e-pa-rt~D~at_e _________________ _ Thermal Activity of Base Course Material Related to December. 1975 Pavement Cracking 6. Performing Organi •ation Code 7. Authorl s) 8. Performing Organi •ation Report No. Samuel H. Carpenter and Robert L. Lytton Research Report No. 18-2 9. Performing Organi !at ion Name and Address 10. Work Unit Na. Texas Transportation Institute Texas A&M University 11. Contract or Grant No. College Station, Texas 77843 Study No. 2-8-73-18 13. Type of Report and Period Covered ~~----------------~--------------------------~12. Sponsoring 'Agency Name and Address Texas State Department of Highways and Public Trans Inter'm1 _ September, 1972 portation; Transportation Planning Division December, 1975 P. 0. Box 5051 14. Sponsoring Agency Code Austin, Texas 78763 15. Supplementary Notes Research performed in cooperation with DOT, FHWA. Study Title: 11 Environmental Deterioration of Pavement 11 16. Abstract Preliminary studies into environmental deterioration of pavements indicated that low-temperature cracking of the asphalt concrete surface was not likely for the west Texas area. -
Flinn Scientific 2019 Purchase Guide a Quick and Easy Checklist of Science Essentials
Flinn Scientific 2019 Purchase Guide A Quick and Easy Checklist of Science Essentials Use this Purchase Guide as a handy tool for: • Taking Inventory • Order Preparation • Budget Management • Future Planning See your Flinn Scientific Catalog/Reference Manual SCIENTIFIC or visit www.flinnsci.com for product details. It’s Easy to Order Tom Trapp from Flinn Scientific! National Account Development Consultant [email protected] www.flinnsci.com/tom-trapp/sa1001 Online 402-960-5578 (mobile) www.flinnsci.com Offering personal assistance to help meet your science curriculum, supply, and lab safety needs. Email [email protected] Quality Products, Fast Delivery, Fax and Low Prices Guaranteed 1-866-452-1436 (toll free) Mail Flinn Scientific, Inc. P.O. Box 219 Batavia, IL 60510-0219 Phone 1-800-452-1261 7:30 am to 5:00 pm CT Monday through Friday Our Guarantee Flinn Scientific, Inc. guarantees that no sale is complete unless the customer is satisfied. Every item we furnish will either conform to the catalog specification, or we will ask your permission, prior to shipment, to ship an alternative product. If you find a lower published nationally advertised catalog price for an identical item, Flinn will “meet or beat” that price. Use this purchase guide containing popular product recommendations ©2019 Flinn Scientific, Inc. All Rights Reserved. to prepare your order, take inventory, and manage your budget. 1 www.flinnsci.com Flinn Scientific 2019 Purchase Guide 1 Item Rec. Item Rec. Product / Item Name Qty 2019 Price Total Product / Item Name Qty 2019 Price Total No. Qty No. Qty Safety & Personal Protection Equipment Aspirator, Water, Polypropylene AP1203 1 $ 19.30 $ - Apron, rubberized, 27" W X 36" L AP7125 30 $ 15.00 $ - Autoclave, Electric, Portable AP1004 1 $ 865.20 $ - Apron, plastic, 30" W x 36" L AP7120 30 $ 7.25 $ - ♦ Balance, Flinn Triple Beam OB2181 $ 115.00 $ - Gloves, Butyl rubber for conc. -
Bunsen Burner Safety
Proper Use of a Bunsen Burner If using a Bunsen burner in a biological safety cabinet, see: http://research.wayne.edu/oehs/pdf/gas-in-bsc.pdf Prior to Lighting Bunsen Burner: • Know the location of the laboratory’s main gas shut off valve and fire extinguisher. • Place the Bunsen burner away from any overhead shelving, equipment, or light fixtures by at least 12 inches. • Remove all papers, notebooks, combustible materials, and excess chemicals from the area. • Tie-back any long hair, dangling jewelry, or loose clothing. • Use correct tubing (butyl rubber hose). Inspect hose for cracks, holes, pinch points, or any defect and replace if necessary. Ensure that the hose fits securely on the gas valve and the burner. • Notify others in the lab that the burner will be in use. Lighting Bunsen Burner: • Before turning on gas, have a sparker/lighter with extended nozzle to ignite burner. Never use matches. • Check connections to burner and desk outlet valve. • Close needle valve and collar. • Open gas valve fully. • Open needle valve 1/2 turn. • Use the spark/lighter to light the flame. • Adjust flame by turning the collar to regulate air flow and produce an appropriate flame for the experiment (typically a medium blue flame). During Use: • Never leave a lighted burner unattended. Drafts (including those created by a hood) may extinguish the flame, but the gas will still be on. This could result in an explosion. • Never face the vessel (test tube, beaker, etc.) being used to heat a material toward yourself or others near the burner. In some experiments, a hot plate may be used in place of the Bunsen burner. -
MT 058 Linkam Stage THMS600 And
Ref : LINKAM – EXAMINA - THMS 600 DESCRIPTION The THMS600 system can be programmed and controlled by either the TMS94,TP94 or CI94 temperature programmers. In order to cool the stage below ambient temperature, the LNP94/2 or LNP94/1 must be used. This manual describes the set up for a THMS600 heating/freezing stage connected to a TMS94 programmer and THM-LNP94/2 automatic cooling pump. The TP94 and CI94 have similar connections to the TMS94 and should be easy to set up following the procedures within this manual. The THM-LNP94/2 is similar to the THM-LNP94/1 THM-LNP94/2 Cooling Pump and Dewar except that the THM-LNP94/2 uses an I 2C cable to communicate with the temperature programmer, so that it automatically selects TMS94 Temperature Programmer the correct pumping speed specific to the programmed rate. THMS600 Microscope Stage If you have any problems with setup or with the operation of the equipment, please contact Linkam Scientific, we will be happy to help. - 1 - Ref : Complete list of THMS600 Accessories Part Part Name Part Description No. 9521 STGLD THMS600 stage connection lead 7017 PVC1 Plastic tube for stage body water cooling 2200 WGI Water/Gas Valve Insert 2202 WVC Water/Gas Valve Connector 2208 SGC Stage Gas Port Valve 9566 VS Complete Valve Set 9573 MGF Set of 6 Magnetic Feet for Mounting Stage 2061 SRR Silicon Rings for Lid and Base 9579 RI17 Stainless Steel Ring Set 2231 TCH Tube Clip Holder (for Nitrogen de-fogging stage lid tube) 2624 ORTHMS Set of O-Rings for THMS Stage Body and Lid 2220 THMS/CC Crucible Carrier for