PAWR Antibody Cat. No.: 27-683

PAWR Antibody

Antibody used in WB on Human Jurkat 0.5-1.0 Antibody used in IHC on Human kidney. ug/ml.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human PAWR.

TESTED APPLICATIONS: ELISA, IHC, WB

PAWR antibody can be used for detection of PAWR by ELISA at 1:62500. PAWR antibody APPLICATIONS: can be used for detection of PAWR by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate

PREDICTED MOLECULAR 37 kDa WEIGHT:

September 27, 2021 1 https://www.prosci-inc.com/pawr-antibody-27-683.html Properties

PURIFICATION: Antibody is supplied as total IgG.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store PAWR STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: PAWR

ALTERNATE NAMES: PAWR, PAR4, Par-4

ACCESSION NO.: NP_002574

PROTEIN GI NO.: 55769533

GENE ID: 5074

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

The tumor suppressor WT1 represses and activates transcription. Anti-Prostate Apoptosis Response Protein Par-4 (PAWR) is a WT1-interacting protein that itself functions as a BACKGROUND: transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells.

REFERENCES: 1) Bergmann, M., et al., (2004) Ann.Hematol.83(10), 646-653.

ANTIBODIES FOR RESEARCH USE ONLY.

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