BAIAP3 (NM 003933) Human Tagged ORF Clone Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC223185

BAIAP3 (NM_003933) Human Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: BAIAP3 (NM_003933) Human Tagged ORF Clone Tag: Myc-DDK Symbol: BAIAP3 Synonyms: BAP3 Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin ORF Nucleotide >RC223185 representing NM_003933 Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC

ATGAGACCCCGGGGAGCAGCGTTTGCAGCGGGCCCGCCAGGTGACCTGCACCTGGGCACCGCCATCGGCT TCGCAGGGGCCATCTGGAGGAGTCGGTCACCCGCCATGTCGACCTTGCTGGACATTAAGAGCAGCGTGCT CAGGCAGGTGCAGGTGTGCCCGTCCTTCCGCCGCAGGACTGAGCAGGACCCAGGGAGTGCCAGCGCCGAC CCGCAGGAGCCTGCCACGGGGGCCTGGAAACCCGGGGATGGCGTGGAGTTCTTTGCCCACATGCGCCTCA TGCTGAAGAAGGGGGAAGGCAGACAGGGCTTGCCGTGCCTCGAGGTCCCCCTGCGCAGTGGCTCGCCAGC ACCCCCGGAGCCTGTGGATCCCAGCCTCGGCCTGAGAGCCCTGGCCCCAGAGGAGGTGGAGATGCTCTAC GAGGAGGCCCTGTACACGGTGCTTTACCGCGCGGGTACCATGGGCCCTGACCAGGTGGACGACGAGGAGG CCCTGCTCAGCTATCTCCAGCAGGTGTTTGGCACCAGCCTTGAGGAGCACACTGAGGCCATCGAGCGAGT GAGGAAGGCCAAGGCCCCCACGTATGCCCTGAAAGTCTCTGTCATGCGTGCCAAGAACCTTCTGGCCAAG GACCCCAACGGCTTCAGCGACCCATACTGCATGCTGGGCATCCTGCCTGCCTCGGACGCCACGCGGGAGC CCCGTGCACAGAAGGAGCAGCGCTTCGGCTTCCGCAAGGGCAGCAAGCGCGGTGGACCCCTGCCTGCCAA GTGCATCCAGGTCACCGAGGTGAAGAGCAGCACCCTGAACCCCGTCTGGAAGGAGCACTTCCTCTTCGAG ATTGAGGATGTGAGCACGGACCAGCTGCACCTGGACATCTGGGATCATGACGACGATGTATCCCTGGTAG AAGCGTGCAGGAAGCTGAATGAAGTCATCGGCCTGAAGGGCATGGGCAGGTACTTCAAACAGATCGTCAA GTCAGCCCGCGCAAACGGGACAGCAGGACCCACCGAGGACCACACCGATGACTTCCTGGGGTGCCTCAAC ATACCTGTCCGGGAGGTGCCTGTGGCTGGCGTCGACCGCTGGTTCAAGCTGGAGCCACGCTCCAGTGCCT CGCGTGTGCAGGGACACTGCCACCTGGTTCTCAAGCTGATCACTACGCAGAGGGATACGGCCATGAGCCA GCGCGGGCGATCCGGCTTCCTGTCCCACCTGCTGCTGCTCAGCCATCTGCTGCGGTTGGAGCACTCAGCA GAGGAGCCCAACTCCAGCAGCTGGCGAGGAGAGCTCAGCACACCAGCCGCCACCATCCTCTGCCTGCACG GAGCCCAGAGCAACCTGTCACCCTTGCAGCTGGCCGTGCTGCACTGGCAGGTCAGCAGCCGCCACCATCA AACCTGCACGCTGGACTACAGCTACCTGCTGGGGCTGCTGGAGGACATGCAGGCACACTGGGAAGAGGCT CCTTCACTGCCCCAGGAGCAGGAGGAGAGCCTGGCTGATAGCCTTTCCGCCTTCTCTGAGTTCGGGCTGC

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 5 BAIAP3 (NM_003933) Human Tagged ORF Clone – RC223185

AGCTGCTGCGCCAGCTCCGAGACTACTTCCCTGCCACCAACAGCACCGCTGTCCACCGCCTGGAGCTGCT GCTGAAGTGTCTGGGCAAGCTGCAGCTCTTCCAACCCTCCTTTGAGATCTGCCCCTTCGAGTCGGAGCTG AACATGGACATTGCTGCGGCCCTGAAGAGAGGCAACCGTGAGTGGTACGACAGGATCCTGAATGACAAGA GTCCCCGAGAGCAGCCAGGACCACAGCGCCTGCCTGGGCTGGTTGTGCTGGCTGACGCCGTCTATGATGA CCTTCAGTTCTGCTACAGTGTGTACGCCAGCCTCTTCCACAGCATCCTCAATGTGGACGTCTTCACCCTG ACCTTCCGGCAGCTGGAGCGTCTGGTGGCTGAGGAGGCGTGGGTGCTGACGGAGGAGCTGAGCCCCAAGA TGACCCTGGAGGTGGCCTCGGGGCTCTTTGAGCTCTACCTGACCCTGGCTGACCTCCAGCGCTTCTGGGA TAGCATCCCTGGCCGGGACAGCCGCTCTCTGGCCCTGGCTGGCATCCACGCCCCCTTCCTGCCTGCTGTG AAGCTCTGGTTCCAAGTGCTGAGGGACCAGGCCAAGTGGAGGCTTCAGGGAGCCGTGGACATGGACACGC TGGAGCCCGTGGACGCCTCCTCCAGGCACAGCAGCTCCGCAGCCACTGCTGGTCTCTGCCTCAGCCACAT CCAGGAGTTGTGGGTGCGCCTGGCGTGGCCTGACCCTGCCCAGGCTCAGGGGCTGGGCACCCAGCTTGGC CAGGACGTGTGTGAGGCCACCCTCTTCTATACGGAGCTGCTTCGGAAGAAGGTGGACACTCAGCCAGGGG CGGCCGGTGAAGCAGTGAGCGAGGCGCTCTGCGTGGTCCTCAACAATGTGGAGCTCGTGCGCAAGGCTGC TGGGCAGGCCTTGAAGGGCCTGGCATGGCCAGAGGGGGCCACGGGGCCCGAGGGGGTGCTCCCCCGCCCT CTGCTCAGCTGCACACAGGCCCTGGACGATGATCTGCAACGGGAGGCCCACACGGTGACAGCGCACCTGA CCTCTAAGATGGTGGGCGACATCCGCAAGTATGTACAGCACATCAGTCTCTCGCCTGACTCCATCCAGAA CGATGAGGCCGTGGCCCCGCTCATGAAGTACCTGGATGAGAAGCTGGCCCTGCTGAACGCCTCGCTGGTG AAGGGGAACCTGAGCAGGGTGCTGGAGGCCCTGTGGGAGCTACTCCTCCAGGCCATTCTGCAGGCGCTGG GTGCAAACCGTGACGTCTCTGCTGATTTCTACAGCCGCTTCCATTTCACGCTGGAGGCCCTGGTCAGTTT TTTCCACGCAGAGGGTCAGGGTTTGCCCCTGGAGAGCCTGAGGGATGGAAGCTACAAGAGGCTGAAGGAG GAGCTGCGGCTGCACAAATGTTCCACCCGCGAGTGCATCGAGCAGTTCTACCTGGACAAGCTCAAACAGA GGACCCTGGAGCAGAACCGGTTTGGACGCCTGAGCGTCCGTTGCCATTACGAGGCGGCTGAGCAGCGGCT GGCCGTGGAGGTGCTGCACGCCGCGGACCTGCTCCCCCTGGACGCCAACGGCTTAAGTGACCCCTTTGTG ATCGTGGAGCTGGGCCCACCGCATCTCTTTCCACTGGTCCGCAGCCAGAGGACCCAGGTGAAGACCCGGA CGCTGCACCCTGTATACGACGAACTCTTCTACTTTTCCGTGCCTGCCGAGGCGTGCCGCCGCCGCGCGGC CTGTGTGTTGTTCACCGTCATGGACCACGACTGGCTGTCCACCAACGACTTCGCTGGGGAGGCGGCCCTC GGCCTAGGTGGCGTCACTGGTGTCGCCCGGCCCCAGGTGGGCGGGGGTGCAAGGGCTGGGCAGCCTGTCA CCCTGCACCTGTGCCGGCCCAGAGCCCAGGTGAGATCTGCGCTGAGGAGGCTGGAAGGCCGCACCAGCAA GGAGGCGCAGGAGTTCGTGAAGAAACTCAAGGAGCTGGAGAAGTGCATGGAGGCGGACCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 5 BAIAP3 (NM_003933) Human Tagged ORF Clone – RC223185

Protein Sequence: >RC223185 representing NM_003933 Red=Cloning site Green=Tags(s)

MRPRGAAFAAGPPGDLHLGTAIGFAGAIWRSRSPAMSTLLDIKSSVLRQVQVCPSFRRRTEQDPGSASAD PQEPATGAWKPGDGVEFFAHMRLMLKKGEGRQGLPCLEVPLRSGSPAPPEPVDPSLGLRALAPEEVEMLY EEALYTVLYRAGTMGPDQVDDEEALLSYLQQVFGTSLEEHTEAIERVRKAKAPTYALKVSVMRAKNLLAK DPNGFSDPYCMLGILPASDATREPRAQKEQRFGFRKGSKRGGPLPAKCIQVTEVKSSTLNPVWKEHFLFE IEDVSTDQLHLDIWDHDDDVSLVEACRKLNEVIGLKGMGRYFKQIVKSARANGTAGPTEDHTDDFLGCLN IPVREVPVAGVDRWFKLEPRSSASRVQGHCHLVLKLITTQRDTAMSQRGRSGFLSHLLLLSHLLRLEHSA EEPNSSSWRGELSTPAATILCLHGAQSNLSPLQLAVLHWQVSSRHHQTCTLDYSYLLGLLEDMQAHWEEA PSLPQEQEESLADSLSAFSEFGLQLLRQLRDYFPATNSTAVHRLELLLKCLGKLQLFQPSFEICPFESEL NMDIAAALKRGNREWYDRILNDKSPREQPGPQRLPGLVVLADAVYDDLQFCYSVYASLFHSILNVDVFTL TFRQLERLVAEEAWVLTEELSPKMTLEVASGLFELYLTLADLQRFWDSIPGRDSRSLALAGIHAPFLPAV KLWFQVLRDQAKWRLQGAVDMDTLEPVDASSRHSSSAATAGLCLSHIQELWVRLAWPDPAQAQGLGTQLG QDVCEATLFYTELLRKKVDTQPGAAGEAVSEALCVVLNNVELVRKAAGQALKGLAWPEGATGPEGVLPRP LLSCTQALDDDLQREAHTVTAHLTSKMVGDIRKYVQHISLSPDSIQNDEAVAPLMKYLDEKLALLNASLV KGNLSRVLEALWELLLQAILQALGANRDVSADFYSRFHFTLEALVSFFHAEGQGLPLESLRDGSYKRLKE ELRLHKCSTRECIEQFYLDKLKQRTLEQNRFGRLSVRCHYEAAEQRLAVEVLHAADLLPLDANGLSDPFV IVELGPPHLFPLVRSQRTQVKTRTLHPVYDELFYFSVPAEACRRRAACVLFTVMDHDWLSTNDFAGEAAL GLGGVTGVARPQVGGGARAGQPVTLHLCRPRAQVRSALRRLEGRTSKEAQEFVKKLKELEKCMEADP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV Chromatograms: https://cdn.origene.com/chromatograms/mk6677_c03.zip Restriction Sites: SgfI-MluI Cloning Scheme:

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 5 BAIAP3 (NM_003933) Human Tagged ORF Clone – RC223185

Plasmid Map:

ACCN: NM_003933 ORF Size: 3561 bp OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

RefSeq: NM_003933.4, NP_003924.2 RefSeq Size: 4523 bp RefSeq ORF: 3564 bp Locus ID: 8938 UniProt ID: O94812 Protein Families: Druggable Genome MW: 131.7 kDa Gene Summary: This p53-target gene encodes a brain-specific angiogenesis inhibitor. The protein is a seven- span transmembrane protein and a member of the secretin receptor family. It interacts with the cytoplasmic region of brain-specific angiogenesis inhibitor 1. This protein also contains two C2 domains, which are often found in proteins involved in signal transduction or membrane trafficking. Its expression pattern and similarity to other proteins suggest that it may be involved in synaptic functions. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 4 / 5 BAIAP3 (NM_003933) Human Tagged ORF Clone – RC223185

Product images:

Western blot validation of overexpression lysate (Cat# [LY418343]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un- transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223185 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).

Coomassie blue staining of purified BAIAP3 protein (Cat# [TP323185]). The protein was produced from HEK293T cells transfected with BAIAP3 cDNA clone (Cat# RC223185) using MegaTran 2.0 (Cat# [TT210002]).