Wageningen Academic Journal of Insects as Food and Feed, 2020; 6(4): 429-440 Publishers Microbial communities and food safety aspects of crickets (Acheta domesticus) reared under controlled conditions X. Fernandez-Cassi1,2*, K. Söderqvist2, A. Bakeeva3, M. Vaga4, J. Dicksved5, I. Vagsholm2, A. Jansson4 and S. Boqvist2 1European Food Safety Authority, EU-FORA fellowship program, Parma, Italy; 2Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Almas allé 8, Uppsala 750 07, Sweden; 3Department of Molecular Sciences, Swedish University of Agricultural Sciences, Uppsala 750 07, Sweden; 4Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala 750 07, Sweden; 5Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Christian Barthels väg 4, Uppsala 756 51, Sweden;
[email protected] Received: 10 October 2019 / Accepted: 4 February 2020 © 2020 Wageningen Academic Publishers OPEN ACCESS RESEARCH ARTICLE Abstract In an approach combining microbiological culture methods with high-throughput sequencing, this study investigated the microbial communities (bacteria, moulds and yeasts) in Swedish-produced edible crickets (Acheta domesticus) reared in a controlled environment. The effects of different feeds on microbial loads and populations in crickets were also studied. The crickets used were third-generation offspring from wild-caught individuals from Sweden, which are adapted to grow in a laboratory environment. The efficiency of rinsing to decrease microbial load was evaluated not obtaining a significant decrease of plating counts for total aerobic counts (TAC) and Enterobacteriaceae. Crickets were divided into three batches and fed different diets (control feed, early-cut red clover hay (ECH), late-cut fresh red clover (LCF)) for 62 days.