The Rule of Medical Laboratories in Covid-19 Outbreak
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The Role Of Medical Laboratories In Covid-19 Outbreak in year 2020 DR. SHOUGI ALAKBARI DIRECTOR GENERAL OF DIAGNOSTIC MEDICINE Department of Diagnostic Medicine - MoPHP 2 Tasks and Responsibility ❑ supervising the work of: ▪ The National Center For Public Health Laboratories (NCPHL) ▪ The National Center For Blood Transfusion And Research (NCBTR) ▪ PCR facilities out of NCPH Labs Facilities / Training Requirements 3 Testing for the presence of SARS-CoV-2 (COVID-19) RNA should be performed in an appropriately equipped laboratory by staff trained to the relevant technical and safety procedures The Facilities 4 PCR units within central laboratories No PCR Unit Provider state 1 NCPHL – Aden WHO Working 2 NCPHL – Taiz WHO Working 3 NCPHL – Sayoon WHO Working 4 NCPHL – Mukalla None Note : It is nessesery to provide NCPHL –Mukalla with PCR facilities The Facilities 5 PCR units of the National AIDS program, which also used in the diagnosis of covid – 19 no PCR Unit provider state 1 Alsadaga Hospital - Aden IOM/GF working 2 Ibn sinna Hospital - Mukalla IOM/GF working Note :The first covid-19 positive case in Yemen is detected in the PCR unit of Ibn sinna hospital - Mukalla in April 2020 The Facilities 6 The New PCR units: Ministry of public health and population has received donation laboratory equipment's and supplies from King Salman Foundation (KSA) and recently from Hail Saeed Anam HSA The Facilities 7 Bellow are the supplies and equipment's provided By Hail Saeed Anam holding group (HAS) on June 2020 no Item Qty Distribution 1 Real-time PCR system 3 Lahj, Abian, Aldalea 2 Nucleic Acid Extractor 3 Lahj, Abian, Aldalea 3 Microcentrifuge 2 Shabwah and Marib 4 Vortex mixer 2 Shabwah and Marib 5 Micropipette different sizes 4 set Shabwah and Marib 6 Dry bath incubator 2 Shabwah and Marib 7 Virus collection kit (swabs+ VTM) 8.500 Aden, Taiz, Sayon, Mukalla, Shabwah, Mareb, Almahra The Facilities 8 The supplies and equipment's provided By King Salman Foundation (KSA): No Item Qty Distribution Shabwa, Marib, Almahra, 1 Real- time PCR thermocycler 5 Alwadiaa, Socotra Shabwa, Marib, Almahra, 2 Automated RNA Extraction machine 5 Alwadiaa, Socotra Shabwa, Marib, Almahra, 3 PCR Diagnostic kits (100 test / kit) 1042 kit Alwadiaa, Socotra Shabwa, Marib, Almahra, 4 RNA Isolation kits (200 test/kit) 504 kit Alwadiaa, Socotra 100,000 Shabwa, Marib, Almahra, 5 Nasal swabs with VTMs pcs Alwadiaa, Socotra Note: the MoPHP and WHO has not been involved in this distribution plan Required Equipment's & consumables not 9 provided by KSA ❑ Bio-safety Cabinet class II ❑ Benchtop microcentrifuge ❑ Vortex Mixer ❑ Adjustable micropipettes (different sizes) ❑ Autoclave ❑ A variety of PCR Plastics Note : All these supplies has been provided from the local market New PCR laboratories 10 In October 2020 the MoPHP starts these new PCR laboratories for testing SARS-COV-2 in governorates of Shabwa, Marib, Alwadiaa and Almahra, after completed all the shortcoming. In Socotra is still not working ▪ Training of staff in these PCR facilities in Shabwah and Almahra was done by : Dr.Shougi Alakbari ▪ Training in Marib and Alwadeah was done by Mohamed Bawahal under a supervision of Dr. Shougi Alakbari Planned PCR laboratories 11 Moreover, MoPHP plans to put more laboratories in Lahj, Abian and Aldalea using supplies donated by Hail Saeed Anam (HAS) during the next few weeks. Planned PCR laboratories 12 In order to make these new PCR labs functional in these new facilities the following equipment's and supplies are mandatory required. No. Item Qty Note 1 Bio-safety Cabinet class II 4 For NA extraction 2 Master mix Cabinet 4 For Master mix preparation 3 Freezer -20oC 4 To maintain PCR kits in a cold chain & sample storage 4 High speed Mico-centrifuge 4 For RNA extraction 5 Vortex mixer 8 For RNA extraction & Master mix preparation 6 Autoclave 4 Is required for decontamination of lab waste 7 Micropipette set 8 For RNA extraction & Master mix preparation, each lab should have at least 2 sets of diff. volumes 8 A variety of PCR Plastics PCR Tubs, pipette Tips of different sizes, Racks etc. SUMMARY 13 Public PCR labs – to check covid-19 suspected cases and travelers PCR labs Distribution Aden, Taiz, Sayon, Mukalla, Working PCR labs. 8 Shabwah, Marib, Almahrah, Alwadeah Planned PCR labs. 4 Lahj, Abian, Aldalea, Socotra 14 Private PCR labs approved by MoPHP – to check only travelers PCR labs Laboratory Name I-lab, Alolagy labs, Almadina In Aden 6 Diag.Center, Azal Spec.labs, King lab and Aden Diagnostic center In Sayon 2 I-lab and Alolagy labs Training of laboratory staff on PCR techniques 15 In October 2020 WHO started training of new NCPHL staff on the diagnosis of COVID-19 by real time PCR techniques Training of laboratory staff on PCR techniques 16 13 – 17 October 2020 WHO conducted training for : ❑ Shabwah ❑ Marib ❑ Almahrah 20 – 24 October : ❑ Lahj ❑ Abian ❑ Aldalae Another set of training is planned for : ❑ Socotra and Alwadiah 17 The training program on PCR techniques held in 2020 No of Governorate T. course participa Trainers venue Program nts Training of 4-5 ▪ Shabwah ▪ Bahir – WHO NCPHL- ▪ NPS sample collection laboratory From ▪ Marib ▪ Fahed - NCPHL Aden ▪ Setting up the lab. For staff on each ▪ Almahrah ▪ Laila - NCPHL COVID-19 test PCR governor ▪ Aden ▪ Shougi - MoPHP ▪ Introduction to PCR – techniques ate ▪ Lahj ▪ Riad - WHO basic principles and ▪ Abian practical steps ▪ Aldalae ▪ Lab. Waste management ▪ taiz ▪ Lab supplies management 18 The World Health Organization (WHO) has provided continued support since the spread of the covid-19 epidemic in Yemen and for every time when we are needed 19.