Personal Care

Consumer Care NAB™ Queen of the Prairie PF A Royal Potion for Youthful-Looking Skin

INCI Name: Water & Spirea ulmaria Extract leaves are oval and toothed with prominent veins running along SAP #: 140220 the undersides. The terminal leaves are tri-lobed and the are off-white to pink, with five tiny petals. The flowers grow in terminal clusters that bloom from June through August. The root of Key Product Attributes the Queen of the Prairie was first used by the Fox Indians for heart troubles and “love potions”. In addition, this was also valued for –– SPF Boosting its astringent properties and used to treat diarrhea, dysentery, and –– Promotes cell renewal to stop bleeding. It is the high tannin content contained within this –– Increases cell respiration in vitro plant that is responsible for this astringent action. –– Astringent properties Not only is this plant rich in tannins, but also in salicylates, specifically salicylic acid. Extracts from Queen of the Prairie were Product Information also utilized to treat the flu, arthritis, and fevers. In the mid 1830s salicylic acid was first isolated from the flowers of this herb, perhaps Queen of the Prairie, also commonly known as the Meadowsweet a forerunner to aspirin and thus the reason it is so effective against and scientifically as rubra, can be found in the flu symptoms and fevers. Today, many herbalists prepare teas from wetsoils, woods and marshes of Northeastern to Midwestern the flowers and roots to treat many of the same ailments mentioned North America. This perennial can grow from 2 to 8 feet tall. The previously. www.lonza.com www.lonza.com/personalcare

Claims Substantiation Photoprotection and Reduction in Erythema The photoprotective quality of NAB™ Queen of the Prairie PF was evaluated In Vitro – Cellular Proliferation and Respiration by exploring the extract’s ability to protect against UV exposure. The study NAB™ Queen of the Prairie PF was tested for cellular proliferation on hu- was performed on five human subjects with normal skin coloration. Each man fibroblast cultures to determine the ability of this natural extract to panelist was exposed to UVA/UVB light in order to determine each individ- influence proliferation. A 0.01 % treatment of NAB™ Queen of the Prairie PF ual’s MED. Following determination of MED, each panelist was exposed to showed an 88 % increase in cell proliferation. The Warburg Assay was em- 1.2 MED – 4 MEDs of UV to the adjacent sites treated with 20 % NAB™ Queen ployed to evaluate skin respiration qualities for NAB™ Queen of the Prairie PF. of the Prairie PF or water. Erythema was measured via Minolta Chroma- Cells were analyzed for oxygen uptake by a Gilson IG-14 differential respiro- meter 24 hours post UV exposure. Results showed a 50 % protection against meter. It was found that 10 % NAB™ Queen of the Prairie PF increased cel- UV exposure. A follow up in vitro study on fibroblasts was also performed lular respiration by 55 %. using NAB™ Queen of the Prairie PF where human fibroblasts were grown in a nutrient rich media. Cultured dishes were then treated with NAB™ In Vivo Cellular Renewal Queen of the Prairie PF, covered with quartz and exposed to UV. Skin cell renewal was tested via Dansyl Chloride. Subjects were treated with 5 % Dansyl Chloride in petrolatum and covered with a patch for 24 hours. Following UV exposure, CFU ability was measured via standard measures. Results indicated that NAB™ Queen of the Prairie PF increased skin cell Results showed that NAB™ Queen of the Prairie PF exposed cells were renewal by 27 % when used at a 10 % concentration. Results may be provided significant protection against UV exposure. viewed in the graph. Typical Properties NAB™ Queen of the Prairie PF Appearance Clear to hazy amber liquid Odor Characteristic 100 Solids (1 gm – 1 hr – 105 °C) 0.4 % Minimum 88

Percent Nitrogen 0.0 – 0.10 % 80 Ash (800 °C) 0.20 % Maximum pH (Direct @ 25 °C) 5.0 – 7.0 60 55 Microbial Content 500 opg Maximum, No Pathogens Recommended Use Level 2 – 10 % 40 27 20

Cell Renewal Fibroblast Proliferation Cell Respiration 10 % Extract 0.01 % Extract 10 % Extract

USA Switzerland This product information corresponds to our knowledge on the subject at the date of publication and Lonza Consumer Care Lonza Ltd we assume no obligation to update it. It is offered without warranty, and is intended for use by per- sons who are experienced and knowledgeable in the field and capable of determining the suitability 70 Tyler Place Muenchensteinerstrasse 38 of ingredients for their specific applications. Because we cannot anticipate all variations in actual end- South Plainfield, NJ 07080 4002 Basel use conditions, we assume no liability and make no warranty in connection with your use of our prod- ucts or product information. We do not guarantee the efficacy of active ingredients, delivery systems, Tel +1 908 561 5200 Tel +41 61 316 81 11 functional ingredients, rheology modifiers, natural or botanical products, preservative and protection systems or proteins in any specific application or use. The information we provide is not intended to substitute for testing. You should perform your own tests to determine for yourself the suitability and efficacy of ingredients in your application and conditions of use. The information we provide should not be construed as a license to operate under or a recommendation to infringe any patent or other intellectual property right, and you should ensure that your use does not infringe any such rights. Our products are for industrial use only. WE MAKE NO WARRANTY (INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR PURPOSE) OF ANY KIND, EXPRESS OR IMPLIED, OTHER THAN THAT OUR PRODUCTS CONFORM TO THE APPLICABLE PRODUCT SPECIFICATIONS.

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Personal Care – NAB™ Queen of the Prairie PF – 11/14