US 20080038290A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2008/0038290 A1 Renimel et al. (43) Pub. Date: Feb. 14, 2008

(54) COSMETIC SLIMMING COMPOSITION (30) Foreign Application Priority Data CONTAINING AN EXTRACT OF BOMASS OF THE ALGANEOCHILORS Aug. 11, 2006 (FR)...... O653364 OLEOABUNDANS Publication Classification (51) Int. Cl. (75) Inventors: Isabelle Renimel, Trainou (FR): Cecile A6IR 36/02 (2006.01) Lamy, Saint Jean De Braye (FR); A6IP 43/00 (2006.01) Delphine Dupont, Dourdan (FR); (52) U.S. Cl...... 424/195.17 Sylvie Darnault, Orleans (FR) (57) ABSTRACT Correspondence Address: The invention relates to a cosmetic composition comprising, as active agent, an extract of biomass of the alga Neochloris HAMRE, SCHUMANN, MUELLER & Oleoabundans, said active agent being incorporated in a LARSON, P.C. cosmetically acceptable vehicle compatible with topical P.O. BOX 2902 application. This extract is advantageously an alcoholic or MINNEAPOLIS, MN 55402-0902 (US) aqueous-alcoholic extract, called the first extract, obtained by treating the biomass with a first alcoholic or aqueous (73) Assignee: LVMH RECHERCHE, Saint Jean De alcoholic solvent, and/or at least one fraction of said first Braye (FR) extract, obtained by fractionating said first extract. The invention further relates to a method of cosmetic treatment (21) Appl. No.: 11/818,125 in order to obtain a slimming effect, wherein a composition as defined above is applied to the appropriate part of the (22) Filed: Jun. 13, 2007 body. US 2008/0038290 A1 Feb. 14, 2008

COSMETC SLMMING COMPOSITION 0017 According to a second feature, the invention relates CONTAINING AN EXTRACT OF BOMASS OF to a process for the preparation of the extracts useful for THE ALGANEOCHLORIS OLEOABUNDANS carrying out the present invention. 0001. The invention relates to the use of an extract of 0018. According to a third feature, it relates to the appli biomass of the alga Neochloris oleoabundans in the field of cation of these same extracts as a slimming agent in the field cosmetics, and more particularly as a slimming agent in a of cosmetics. cosmetic composition. 0019. According to the essential characteristic of its first 0002) Numerous uses of microorganisms in cosmetic or feature, the invention relates to a cosmetic composition dermatological compositions are known. comprising, as active agent, an extract of biomass of the alga Neochloris Oleoabundans, said active agent being incorpo 0003. The taxonomic information concerning the alga rated in a cosmetically acceptable vehicle compatible with Neochloris Oleoabundans is given below: topical application. 0004 Division: Chlorophyta 0020. As explained earlier, the extracts of biomass of the 0005 Class: Chlorophyceae alga Neochloris Oleoabundans have valuable slimming 0006 Order: Chlorococcales properties when they are applied to the skin. 0021. There are various culture conditions that produce 0007 Family: Chlorococcaceae the biomass used according to the invention. However, the 0008 Genus: Neochloris alga Neochloris Oleoabundans will preferably be cultivated in a culture medium based on seawater or a brackish water 0009 Species: Oleoabundans with a sodium chloride content of between 10 g/l and 30 g/l. 0010 This alga is a microalga forming part of the numer preferably of between 16 g/1 and 20 g/l, this medium also ous microorganisms and algae cited in international patent optionally containing nutrients that are conventionally used. application WO 2006/008401, which describes a process for the preparation of a clarified culture medium of at least one 0022 Particularly advantageously, the sodium chloride photosynthetic marine and/or freshwater microorganism, content will be 18 g/l. and the use of these clarified culture media, especially in the 0023 The culture medium advantageously comprises field of cosmetics. brackish water, i.e. a mixture of seawater and fresh water. 0011 Said document expresses no interest in the use of 0024. The seawater used will preferably be taken from the biomass itself, but only in the use of the clarified culture the English Channel along the northern coast of Brittany, medium. particularly in the Roscoff region (Finistere, France). 0012. The literature so far contains no indications of the 0025. As explained earlier, the extracts used to prepare use of extracts of biomass of the alga Neochloris Oleoabun the cosmetic compositions of the invention will advanta dans as an active agent of a cosmetic composition. geously be alcoholic or aqueous-alcoholic extracts or 0013 The experiments carried out by the inventors of the extracts resulting from a fractionation of the latter. present invention on different extracts of biomass of this alga 0026. In the description which follows, first extract is showed that they were of very particular value in the field of understood as meaning the alcoholic or aqueous-alcoholic cosmetics, especially as a slimming agent. extract obtained by treating the biomass with a first alcoholic 0014) More precisely, it was apparent to the inventors of or aqueous-alcoholic solvent. the present invention that the extracts of biomass of the alga 0027. In one advantageous variant of the invention, this Neochloris Oleoabundans obtained by means of polar and/or first extract, which has valuable slimming properties, is used apolar solvents exhibited very valuable slimming properties as a slimming agent in the compositions of the invention. when they were applied topically to the skin, particularly in cosmetic compositions. 0028. In other variants, the active agent used in the cosmetic compositions of the invention is a fraction obtained 0015. It was apparent, however, that certain extracts of by fractionating this first extract. this alga proved particularly valuable for their slimming properties. This is the case of extracts obtained by means of 0029. In yet another variant, a mixture of the first extract, polar solvents, particularly alcoholic or aqueous-alcoholic as defined above, and at least one fraction of this first extract, extracts. Furthermore, it was also apparent that these obtained by fractionation thereof, may be used in the com extracts obtained by means of polar solvents, particularly position of the invention. alcoholic or aqueous-alcoholic extracts, could Subsequently 0030 The first extract will advantageously be prepared be subjected to various fractionation operations that made it using a first alcoholic or aqueous-alcoholic solvent in which possible in particular to obtain novel extracts, said extracts themselves also exhibiting valuable slimming properties the alcohol is selected from the group consisting of C to Cs while at the same time having organoleptic properties or a monoalcohols, C. to Ce glycols and mixtures thereof. coloration that are distinctly improved compared with the 0031 Methanol, ethanol and butylene glycol may be first alcoholic or aqueous-alcoholic extracts. mentioned in particular as examples of these alcohols. 0016. Thus, according to a first feature, the invention 0032. The composition of said first solvent can vary relates to a cosmetic composition comprising an extract of within very wide limits of water and alcohol. However, in biomass of the alga Neochloris Oleoabundans. the case of a mixture of ethanol and water, the respective US 2008/0038290 A1 Feb. 14, 2008

proportions of these two constituents will be chosen in the the composition of the invention in the form of a solution in range from 100% to 40% by volume of ethanol and 0% to a mixture of water and butylene glycol and particularly in 60% of water. the form of a solution containing from 0.05 to 5% by weight and preferably from 0.1 to 1% by weight of dry extract, 0033. As explained above, the first extract obtained by based on the weight of the water/butylene glycol mixture. extraction of the biomass with an alcoholic or aqueous alcoholic Solvent has particularly valuable slimming prop 0047. This will advantageously be done using a 50/50 by erties, making it a particularly valuable extract as an active Volume mixture of water and butylene glycol. agent in terms of the invention. 0048. According to a second feature, the invention relates 0034. As also explained above, especially for the purpose to a process for the preparation of an extract of Neochloris of improving the organoleptic properties of the extract oleoabundans intended especially for the preparation of a and/or reducing its coloration, the first extract may be composition as defined above. This process comprises at Subjected to various fractionation operations to give novel least one step for extraction of the biomass with a first extracts with improved properties, particularly improved alcoholic or aqueous-alcoholic solvent, as defined above, to organoleptic properties and/or a reduced coloration. give a first extract. 0035) In one advantageous variant of the invention, the 0049. This process advantageously also comprises at first extract is subjected to a partition step by liquid-liquid least one step for fractionation of this first extract. extraction between a second aqueous or aqueous-alcoholic solvent and a third solvent that is immiscible with the second 0050. In one advantageous variant, this fractionation of solvent and has a polarity of between 0 and 4.7, defined by the first extract consists of a step for partition of the first its polarity coefficient P, which produces an aqueous-alco extract between a second aqueous-alcoholic Solvent and a holic phase comprising a first fraction of the first extract, third solvent that is immiscible with the second solvent and called the second extract, and a phase consisting of the third has a polarity P of between 0 and 4.7, said partition Solvent and comprising a second fraction of the first extract, producing a second and a third extract respectively con called the third extract. tained in each of the phases made up of the second and third Solvents. 0036). For the definition of the polarity coefficient P', reference may be made to the publication by L. R. SNYDER 0051 Finally, the process of the invention may comprise entitled “Classification of the solvent properties of common additional conventional steps for purification of the different liquids' in Journal of Chromatography 92 (1974) 223-230. extracts for the purpose of improving their organoleptic qualities or their coloration. 0037 For this fractionation step, the second solvent advantageously consists of a mixture of water and an alcohol 0052 Examples of such steps which may be mentioned selected from the group consisting of C to Cs monoalcohols are steps for decolorization over activated charcoal, e.g. and C2 to Ce glycols. XCV charcoal, or filtration steps, e.g. with a filter cut-off of 0038 Methanol, ethanol, butylene glycol and mixtures between 40 and 0.20 um. thereof will be chosen as the preferred alcohol. 0053) The invention further relates to the use of the extracts or mixtures of extracts as defined above, or as 0039 Those skilled in the art will understand that the obtained by the process defined above, as a slimming agent proportions of water and alcohol depend on the nature of the in a cosmetic composition or for the manufacture of a alcohol, but also on that of the third solvent. cosmetic composition. 0040. If the second solvent is aqueous-alcoholic and comprises ethanol, the latter is advantageously present in a 0054 The results given in the Examples which follow proportion of at least 20% by volume. clearly illustrate the lipolytic activity of the extracts accord ing to the invention by monitoring the release of fatty acids 0041. The third solvent, which is immiscible with the in situ from skin explants. second solvent and has a polarity P" of between 0 and 4.7, is advantageously selected from the group consisting of chlo 0055 Finally, according to a final feature, the invention roform, ethyl acetate, diethyl ether and mixtures thereof. relates to a method of cosmetic care of the human body in order to obtain a slimming effect. This method comprises the 0042. As explained earlier, the second and/or third application of a composition as defined above to a part of the extract, as defined above, will advantageously be chosen as body in need thereof. an extract constituting an active agent in terms of the invention. 0056 Such a method comprises selecting the part of the body that requires a slimming treatment, and applying a 0043. The proportions of extract(s) comprised in the composition of the invention to this part of the body. compositions of the invention can vary within very wide limits. 0057 The Examples which follow illustrate the invention 0044) However, these proportions will advantageously be without implying a limitation. between 0.0003 and 2% by weight, based on the total weight of the composition, the percentage being expressed as dry EXAMPLES extract(s). I. Culture of the Alga Neochloris Oleoabundans and Recov 0045. These proportions are advantageously between ery of the Biomass 0.003 and 0.3% by weight. 0058. The strain used originates from the UTEX algoth 0046. Furthermore, in one particularly advantageous eque (algae collection of the University of Texas in Austin) embodiment, even if the extract is not obtained by extraction and has the reference UTEX 1185. This strain was isolated with butylene glycol, it proves valuable to introduce it into in Saudi Arabia. US 2008/0038290 A1 Feb. 14, 2008

0059. In the context of the Example, the culture was resolubilized by adding 100 ml of distilled water, frozen and effected in batch mode in a 20 m tank filled with brackish then lyophilized to give an extract, which is hereafter water containing 18 g/l of salt, with the addition of a nutrient denoted as A1. medium of the following composition: 0081 a.2. Following the same extraction scheme as that 0060 Na-EDTA: 4.36.10 g/1 described for the preparation of extract A in item 1.a), the filtration residue obtained by extraction with methanol is in 0061 FeC1.6HO: 1.58.10 g/1 this case resolubilized in 100 ml of chloroform, and 95 ml 0062 NaHCO: 0.6.10 g/1 of water and 5 ml of methanol are added. A liquid-liquid partition is effected between the two phases. The organic 0063 MnC14HO: 0.36.10 g/1 phase is discarded and the aqueous phase is rewashed twice 0064 CuSO4.5HO: 1.10 g/1 with 50 ml of chloroform and then it too is discarded for the preparation of the extract described in item a.3) below. The 0065 ZnSO.7HO: 2.2.10 g/1 organic phases are pooled and then evaporated to dryness on a rotary evaporator, resolubilized in 50 ml of water and 0.066 CoC1.6HO: 1.10 g/1 lyophilized. The resulting extract is hereafter denoted as A2. 0067 MgSO: 4.95.10 g/1 0082 a.3. The aqueous phase as defined in item a.2) is 0068 NaNO: 0.17 g/1 evaporated on a rotary evaporator, resolubilized in 50 ml of water and lyophilized to give an extract, which is hereafter 0069. HBO, 2.47.10 g/1 denoted as A3. 0070 NaHCO: 4.23.10 g/1 2. Preparation of an Extract B by a First Aqueous-Ethanolic 0071 KHPO, 3.475.10.2 g/1 Extraction and Fractionation of this Extract 0072 CaC1: 2.94.10 g/1 0083) a. Preparation of an Aqueous-Ethanolic Extract B 0084. 250 ml of an ethanol/water mixture (94/4 volume/ 0073 Vitamin B: 1.10 g/1 volume, hereafter V/v) are added to 100 g of algal biomass 0074) Vitamin B: 5.107 g/1 containing 14.6% of dry extract in a 500 ml round-bottomed flask. The mixture is refluxed for 30 minutes. After cooling, 0075) Vitamin H: 5.107 g/1 the extract is centrifuged at 4000 rpm for 20 minutes and the 0076. It is also possible to employ a semicontinuous centrifugation residue is then re-extracted a total of 3 times mode. In the type of tank used, the culture reaches maturity with 250 ml of aqueous-alcoholic solution. after 12 to 15 days. 0085. The Supernatants are subsequently pooled, filtered on a Bichner funnel using a 0.40 um filter (GF/F, Whatman) 0077. At the end of this period, either all or part of the and then evaporated to dryness on a rotary evaporator. algal Suspension is withdrawn. 0086) The resulting extract B is then dissolved in DMSO 0078. The biomass is then concentrated by centrifugation at a concentration of 5% (weight/volume, hereafter w/v) for with an acceleration of 14000 g (1 g=9.18 m/s). the biological tests. II. Preparation of Extracts According to the Invention 0087 b. Fractionation of Extract B by Liquid-Liquid 1. Preparation of Extracts from a First Extraction with Partition to Prepare an Extract B1 and an Extract B2 Methanol 0088 2 g of the previous extract B are diluted in about 80 0079 a. 250 ml of methanol are added to 100 g of algal ml of an ethanol/water mixture (20/40, V/v) to give a 2.5% biomass containing 26% of dry extract in a 500 ml round solution. 53 ml of ethyl acetate are added to form a two bottomed flask. The mixture is heated at 65° C. for 30 phase system. The two phases are separated in order to minutes under nitrogen. The whole is centrifuged at 400 rpm recover the first organic phase. The aqueous phase is for 10 minutes. The liquid phase is separated from the solid rewashed with 53 ml of ethyl acetate and this is repeated a residue and discarded. The filtration residue is then trans total of 4 times. The 4 organic phases are pooled and then ferred to a 250 ml round-bottomed flask and re-extracted evaporated on a rotary evaporator to give an extract, which with 250 ml of methanol under the same conditions. This is hereafter called extract B1. operation is repeated one final time. The three liquid phases 0089. The aqueous phase is also dried on a rotary evapo are pooled and then evaporated to dryness on a rotary rator to give a dry extract, which is hereafter called extract evaporator. The solids obtained are resolubilized in 100 ml B2. of water and then frozen and lyophilized. This extract is 0090 Extract B1 is solubilized in DMSO at a concentra ultimately in the form of a powder and is hereafter denoted tion of 5% (w/v) and extract B2 is dissolved in water at a as extract A. concentration of 5% (w/v) for the biological tests. 0080 a.1. Following the same preparatory scheme as that described in item a), the liquid phase, which is again 3. Preparation of an Extract C by a First Aqueous-Ethanolic collected, is evaporated to dryness on a rotary evaporator Extraction and then resolubilized, but this time with 200 ml of heptane. 0.091 250 ml of an ethanol/water mixture (96/4, v/v) are The whole is treated with ultrasound and then centrifuged added to 100 g of algal biomass containing 12% of dry for 30 minutes at 4000 rpm. The supernatant is recovered, extract in a 500 ml round-bottomed flask. The mixture is evaporated to dryness on a rotary evaporator and then refluxed for 30 minutes. After cooling, the extract is centri US 2008/0038290 A1 Feb. 14, 2008 fuged at 400 rpm for 20 minutes and the centrifugation 0110 Assay of the Free Fatty Acids Present in the residue is then re-extracted a total of 3 times with 250 ml of Medium: aqueous-alcoholic Solution. 0111. After extraction of the culture medium, the free 0092. The supernatants are subsequently pooled, filtered fatty acids present therein are separated off and assayed by on a Bichner funnel (GF/F filter, Whatman, 40 um) and then high performance thin layer chromatography. evaporated to dryness on a rotary evaporator. The resulting dry extract is extract C. It is then dissolved in DMSO at a III 3. Results concentration of 5% (w/v) for the biological tests. 0.112. The viability and morphology of the adipocytes are III. Assay of the Lipolytic Activity of the Extracts of the monitored by histology. After being kept alive for 8 days, the Invention reference and treated explants exhibit neither visible degra dation nor cell necrosis. III 1. Principle of the Assay 0113. The lipolytic activity is evaluated by determining 0093. The object is to evaluate the lipolytic activity of the proportions of free fatty acids released into the culture extracts of Neochloris Oleoabundans according to the inven medium. tion on explants of adipose tissues that are kept alive. 0114. The results obtained are given in the Tables below 0094. The different extracts are incorporated into the and correspond to the weights of fatty acids released into the culture medium under the conditions described below. culture medium during the eight days of treatment, 0.095. After a contact time of 8 days, the activity is expressed in Jug. evaluated by assaying the fatty acids released into the a. Results Obtained with Extract B According to the Inven culture medium. tion III 2. Procedure Fatty Acids Released 0096 Explants: (weights in Lig) 0097 Preparation of 9 explants of adipose tissues and keeping them alive in BEM (BIO-EC's Explants Medium) 0115) 0098 Division of the explants into 3 batches of 3 explants: Mean Standard deviation 0099 a reference batch, in the presence of the culture Reference 8.7 1.4 medium Caffeine 0.1% 46.9 6.7 Extract B 0.01% 16.2 2.8 0100 a batch treated with caffeine as a positive refer CC b. Results Obtained with Extract B1 (Ethyl Acetate Fraction) 0101 a batch treated with an extract according to the invention Fatty Acids Released 0102 Test Products: (weights in Lig) 0.103 Positive reference: The caffeine is used at a final concentration of 0.1% (1 mg/ml) in the survival 0.116) medium, i.e. at a concentration ten times greater than that of the extracts according to the invention, the Mean Standard deviation caffeine no longer having a lipolytic effect at a con Reference 3.6 O.6 centration of 0.01% (100 g/ml). Caffeine 0.1% 12.9 1.8 0.104 Extract of Neochloris according to the invention: Extract B1 O.O1% 20.8 3.6 The extract is in the form of a dry powder which is solubilized in DMSO at a concentration of 50% and then diluted to /500 in the explant culture medium so as c. Results Obtained with Extract B2 (Aqueous-Ethanolic to be tested at a final concentration of 0.01% (100 Fraction) g/ml). Fatty Acids Released 0105. Application of the Extracts: (weights in Lig) 0106. At D0 the explants are kept alive in 2 ml of culture medium in which the test extract is incorporated. 0117) 0107 This treatment is repeated at D2, D4 and D6. 0108 Samplings: Mean Standard deviation Reference 3.6 O.6 0109). At D2, D4, D6 and D8 the culture medium is Caffeine 0.1% 12.9 1.8 sampled. For each explant the media sampled at D2, D4, D6 Extract B2 O.O1% 10.8 1.3 and D8 are combined in the same tube and stored at -20°C. for assaying the fatty acids present in the medium. US 2008/0038290 A1 Feb. 14, 2008

III 4. Conclusions 2. The composition according to claim 1, wherein the 0118. It is seen that all the test extracts according to the biomass is obtained by cultivation of the alga Neochloris invention have a significant lipolytic activity inasmuch as a oleoabundans in a culture medium based on seawater or a Substantial amount of fatty acid is released into the medium. brackish water with a sodium chloride content of between 10 This lipolytic activity can even be considered as very g/l and 30 g/l, said medium also optionally comprising significant, particularly that of extract Bi, because at the nutrients. 0.01% dose used for the extracts according to the invention, 3. The composition according to claim 2, wherein the caffeine, a positive reference well known for its lipolytic sodium chloride content of said culture medium is 18 g/l. activity, is no longer active. 4. The composition according to claim 2, wherein the culture medium comprises a brackish water. IV. Formulation Examples 5. The composition according to claim 1, wherein said 0119) The topical cosmetic compositions described extract is an alcoholic or aqueous-alcoholic extract, called below are prepared in conventional manner from the fol the first extract, obtained by treating the biomass with a first lowing centesimal compositions, expressed by weight. alcoholic or aqueous-alcoholic solvent, and/or at least one fraction of said first extract, obtained by fractionating said Slimming gel first extract. 6. The composition according to claim 5, wherein the Deionized water 73.5% alcohol of said first solvent is an alcohol selected from the Alcohol 96.2 vol% 21 AMPS polymer (Sepigel 305) 3 group consisting of C-C monoalcohols, C-C glycols and Preservative O.3 mixtures thereof. Perfume concentrate O.1 7. The composition according to claim 6, wherein said Extract of Neochloris oleoabundans 2 alcohol is methanol, ethanol or butylene glycol. (extract B1 at 3% in wateributylene glycol, 50/50) Sodium hyaluronate (high molecular weight) O.1 8. The composition according to claim 5, wherein said Slimming cream first solvent comprises 100% to 40% by volume of ethyl alcohol and 0% to 60% by volume of water. Glyceryl Stearate + PEG-100 stearate 6.0% Hydrogenated polyisobutene 3.0 9. The composition according to claim 5, wherein said Squalane 3.0 fractionation results from a partition step by liquid-liquid Glyceryl caprylate caprate triglycerides 3.0 extraction of said first extract between a second aqueous or Glycerol 2.O Octyl methoxycinnamate 2.O aqueous-alcoholic solvent and a third solvent that is immis Cetylstearyl octanoate 1.5 cible with the second solvent and has a polarity of between Beeswax 1.5 0 and 4.7, defined by its polarity coefficient P', which Cetyl alcohol 1.O produces an aqueous-alcoholic phase comprising a first Stearyl alcohol 1.O fraction of the first extract, called the second extract, and a Dimethicone 1.O Xanthan gum O.2 phase consisting of the third solvent and comprising a Extract of Neochloris oleoabundans 2 second fraction of the first extract, called the third extract. (extract B2 at 3% in wateributylene glycol, 50/50) 10. The composition according to claim 9, wherein the Preservatives, perfume, colorants O.2 Water qSp second solvent is an aqueous-alcoholic solvent, the alcohol Fluid slimming emulsion of this second solvent being selected from the group con sisting of C-C monoalcohols, C-C glycols and mixtures Octyl palmitate 7.0% thereof. Glyceryl caprylate caprate triglycerides 3.0 Octyl octanoate 2.O 11. The composition according to claim 10, wherein said Phenyl trimethicone 2.O alcohol is methanol, ethanol or butylene glycol. Glycerol 2.O 12. The composition according to claim 10, wherein said Stearic acid 1.O Sorbitan 1.O alcohol is ethanol and is present in said aqueous-alcoholic Cetyl alcohol O.S solvent in a proportion of at least 20% by volume. Stearyl alcohol O.S 13. The composition according to claim 9, wherein said Extract C of Neochloris oleoabundans 1 third solvent is selected from the group consisting of chlo Preservatives, perfume, colorants, neutralizer O.1 Water qSp roform, ethyl acetate, diethyl ether and mixtures thereof. Slimming lotion 14. The composition according to claim 1, wherein said extract is the first extract as defined in claim 5. Butylene glycol 3% EDTA O.1 15. The composition according to claim 1, wherein the Solubilizer 1 extract is the second extract as defined in one of claim 10. Perfume concentrate O.1 16. The composition according to claim 1, wherein the Alcohol 5.2 extract is the third extract as defined in claim 10. Extract A of Neochloris oleoabundans O.S Benzophenone-4 O.13 17. The composition according to claim 1, wherein it Preservatives, perfume, colorants, neutralizer O.1 comprises at least one first extract and/or second extract Water qSp and/or third extract as defined in claim 5. 18. The composition according to claim 1, wherein it comprises from 0.003 to 2% by weight, expressed as solids, 1. A cosmetic composition, comprising, as active agent, based on the weight of said composition of Neochloris an extract of biomass of the alga Neochloris oleoabundans, Oleoabundans biomass extract. said active agent being incorporated in a cosmetically 19. The composition of claim 1, which comprises said acceptable vehicle compatible with topical application. extract in efficient amount to obtain a slimming effect. US 2008/0038290 A1 Feb. 14, 2008

20. A process for the preparation of an extract of Neochlo second aqueous-alcoholic or aqueous solvent and a third ris Oleoabundans which comprises at least one step for solvent that is immiscible with the second solvent and has a extraction of biomass of the alga Neochloris Oleoabundans polarity P" of between 0 and 4.7, said partition producing a with a first alcoholic or aqueous-alcoholic Solvent, as second and a third extract respectively contained in each of defined in claim 6, to give a first extract. the phases made up of the second and third solvents. 21. The process according to claim 20, wherein it also 23. A method providing a slimming effect for a human comprises at least one step for fractionation of said first body, which comprises application of a composition as eXtract. defined in claim 1 to a part of the body in need thereof. 22. The process according to claim 21, wherein it com prises a step for partition of said first extract between a k k k k k