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(12) Patent Application Publication (10) Pub. No.: US 2008/0038290 A1 Renimel Et Al

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(12) Patent Application Publication (10) Pub. No.: US 2008/0038290 A1 Renimel Et Al US 20080038290A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2008/0038290 A1 Renimel et al. (43) Pub. Date: Feb. 14, 2008 (54) COSMETIC SLIMMING COMPOSITION (30) Foreign Application Priority Data CONTAINING AN EXTRACT OF BOMASS OF THE ALGANEOCHILORS Aug. 11, 2006 (FR).............................................. O653364 OLEOABUNDANS Publication Classification (51) Int. Cl. (75) Inventors: Isabelle Renimel, Trainou (FR): Cecile A6IR 36/02 (2006.01) Lamy, Saint Jean De Braye (FR); A6IP 43/00 (2006.01) Delphine Dupont, Dourdan (FR); (52) U.S. Cl. ........................................................ 424/195.17 Sylvie Darnault, Orleans (FR) (57) ABSTRACT Correspondence Address: The invention relates to a cosmetic composition comprising, as active agent, an extract of biomass of the alga Neochloris HAMRE, SCHUMANN, MUELLER & Oleoabundans, said active agent being incorporated in a LARSON, P.C. cosmetically acceptable vehicle compatible with topical P.O. BOX 2902 application. This extract is advantageously an alcoholic or MINNEAPOLIS, MN 55402-0902 (US) aqueous-alcoholic extract, called the first extract, obtained by treating the biomass with a first alcoholic or aqueous (73) Assignee: LVMH RECHERCHE, Saint Jean De alcoholic solvent, and/or at least one fraction of said first Braye (FR) extract, obtained by fractionating said first extract. The invention further relates to a method of cosmetic treatment (21) Appl. No.: 11/818,125 in order to obtain a slimming effect, wherein a composition as defined above is applied to the appropriate part of the (22) Filed: Jun. 13, 2007 body. US 2008/0038290 A1 Feb. 14, 2008 COSMETC SLMMING COMPOSITION 0017 According to a second feature, the invention relates CONTAINING AN EXTRACT OF BOMASS OF to a process for the preparation of the extracts useful for THE ALGANEOCHLORIS OLEOABUNDANS carrying out the present invention. 0001. The invention relates to the use of an extract of 0018. According to a third feature, it relates to the appli biomass of the alga Neochloris oleoabundans in the field of cation of these same extracts as a slimming agent in the field cosmetics, and more particularly as a slimming agent in a of cosmetics. cosmetic composition. 0019. According to the essential characteristic of its first 0002) Numerous uses of microorganisms in cosmetic or feature, the invention relates to a cosmetic composition dermatological compositions are known. comprising, as active agent, an extract of biomass of the alga Neochloris Oleoabundans, said active agent being incorpo 0003. The taxonomic information concerning the alga rated in a cosmetically acceptable vehicle compatible with Neochloris Oleoabundans is given below: topical application. 0004 Division: Chlorophyta 0020. As explained earlier, the extracts of biomass of the 0005 Class: Chlorophyceae alga Neochloris Oleoabundans have valuable slimming 0006 Order: Chlorococcales properties when they are applied to the skin. 0021. There are various culture conditions that produce 0007 Family: Chlorococcaceae the biomass used according to the invention. However, the 0008 Genus: Neochloris alga Neochloris Oleoabundans will preferably be cultivated in a culture medium based on seawater or a brackish water 0009 Species: Oleoabundans with a sodium chloride content of between 10 g/l and 30 g/l. 0010 This alga is a microalga forming part of the numer preferably of between 16 g/1 and 20 g/l, this medium also ous microorganisms and algae cited in international patent optionally containing nutrients that are conventionally used. application WO 2006/008401, which describes a process for the preparation of a clarified culture medium of at least one 0022 Particularly advantageously, the sodium chloride photosynthetic marine and/or freshwater microorganism, content will be 18 g/l. and the use of these clarified culture media, especially in the 0023 The culture medium advantageously comprises field of cosmetics. brackish water, i.e. a mixture of seawater and fresh water. 0011 Said document expresses no interest in the use of 0024. The seawater used will preferably be taken from the biomass itself, but only in the use of the clarified culture the English Channel along the northern coast of Brittany, medium. particularly in the Roscoff region (Finistere, France). 0012. The literature so far contains no indications of the 0025. As explained earlier, the extracts used to prepare use of extracts of biomass of the alga Neochloris Oleoabun the cosmetic compositions of the invention will advanta dans as an active agent of a cosmetic composition. geously be alcoholic or aqueous-alcoholic extracts or 0013 The experiments carried out by the inventors of the extracts resulting from a fractionation of the latter. present invention on different extracts of biomass of this alga 0026. In the description which follows, first extract is showed that they were of very particular value in the field of understood as meaning the alcoholic or aqueous-alcoholic cosmetics, especially as a slimming agent. extract obtained by treating the biomass with a first alcoholic 0014) More precisely, it was apparent to the inventors of or aqueous-alcoholic solvent. the present invention that the extracts of biomass of the alga 0027. In one advantageous variant of the invention, this Neochloris Oleoabundans obtained by means of polar and/or first extract, which has valuable slimming properties, is used apolar solvents exhibited very valuable slimming properties as a slimming agent in the compositions of the invention. when they were applied topically to the skin, particularly in cosmetic compositions. 0028. In other variants, the active agent used in the cosmetic compositions of the invention is a fraction obtained 0015. It was apparent, however, that certain extracts of by fractionating this first extract. this alga proved particularly valuable for their slimming properties. This is the case of extracts obtained by means of 0029. In yet another variant, a mixture of the first extract, polar solvents, particularly alcoholic or aqueous-alcoholic as defined above, and at least one fraction of this first extract, extracts. Furthermore, it was also apparent that these obtained by fractionation thereof, may be used in the com extracts obtained by means of polar solvents, particularly position of the invention. alcoholic or aqueous-alcoholic extracts, could Subsequently 0030 The first extract will advantageously be prepared be subjected to various fractionation operations that made it using a first alcoholic or aqueous-alcoholic solvent in which possible in particular to obtain novel extracts, said extracts themselves also exhibiting valuable slimming properties the alcohol is selected from the group consisting of C to Cs while at the same time having organoleptic properties or a monoalcohols, C. to Ce glycols and mixtures thereof. coloration that are distinctly improved compared with the 0031 Methanol, ethanol and butylene glycol may be first alcoholic or aqueous-alcoholic extracts. mentioned in particular as examples of these alcohols. 0016. Thus, according to a first feature, the invention 0032. The composition of said first solvent can vary relates to a cosmetic composition comprising an extract of within very wide limits of water and alcohol. However, in biomass of the alga Neochloris Oleoabundans. the case of a mixture of ethanol and water, the respective US 2008/0038290 A1 Feb. 14, 2008 proportions of these two constituents will be chosen in the the composition of the invention in the form of a solution in range from 100% to 40% by volume of ethanol and 0% to a mixture of water and butylene glycol and particularly in 60% of water. the form of a solution containing from 0.05 to 5% by weight and preferably from 0.1 to 1% by weight of dry extract, 0033. As explained above, the first extract obtained by based on the weight of the water/butylene glycol mixture. extraction of the biomass with an alcoholic or aqueous alcoholic Solvent has particularly valuable slimming prop 0047. This will advantageously be done using a 50/50 by erties, making it a particularly valuable extract as an active Volume mixture of water and butylene glycol. agent in terms of the invention. 0048. According to a second feature, the invention relates 0034. As also explained above, especially for the purpose to a process for the preparation of an extract of Neochloris of improving the organoleptic properties of the extract oleoabundans intended especially for the preparation of a and/or reducing its coloration, the first extract may be composition as defined above. This process comprises at Subjected to various fractionation operations to give novel least one step for extraction of the biomass with a first extracts with improved properties, particularly improved alcoholic or aqueous-alcoholic solvent, as defined above, to organoleptic properties and/or a reduced coloration. give a first extract. 0035) In one advantageous variant of the invention, the 0049. This process advantageously also comprises at first extract is subjected to a partition step by liquid-liquid least one step for fractionation of this first extract. extraction between a second aqueous or aqueous-alcoholic solvent and a third solvent that is immiscible with the second 0050. In one advantageous variant, this fractionation of solvent and has a polarity of between 0 and 4.7, defined by the first extract consists of a step for partition of the first its polarity coefficient P, which produces an aqueous-alco extract between a second aqueous-alcoholic Solvent and a holic phase comprising a first fraction of the first extract, third solvent that is immiscible with the second solvent and called the second extract, and a phase consisting of the third has a polarity P of between 0 and 4.7, said partition Solvent and comprising a second fraction of the first extract, producing a second and a third extract respectively con called the third extract. tained in each of the phases made up of the second and third Solvents. 0036). For the definition of the polarity coefficient P', reference may be made to the publication by L.
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