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Developmental Regulation and Function of AMPA Receptor Subunits in Chicken Lumbar Motoneurons Xianglian Ni University of Vermont

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University of Vermont ScholarWorks @ UVM Graduate College Dissertations and Theses Dissertations and Theses 2009 Developmental Regulation and Function of AMPA Receptor Subunits in Chicken Lumbar Motoneurons Xianglian Ni University of Vermont Follow this and additional works at: https://scholarworks.uvm.edu/graddis Recommended Citation Ni, Xianglian, "Developmental Regulation and Function of AMPA Receptor Subunits in Chicken Lumbar Motoneurons" (2009). Graduate College Dissertations and Theses. 161. https://scholarworks.uvm.edu/graddis/161 This Dissertation is brought to you for free and open access by the Dissertations and Theses at ScholarWorks @ UVM. It has been accepted for inclusion in Graduate College Dissertations and Theses by an authorized administrator of ScholarWorks @ UVM. For more information, please contact [email protected]. DEVELOPMENTAL REGULATION AND FUNCTION OF AMPA RECEPTOR SUBUNITS IN CHICKEN LUMBAR MOTONEURONS A Dissertation Presented by Xianglian Ni to The Faculty of the Graduate College of The University of Vermont In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy Specializing in Biology October, 2009 Accepted by the Facult!, of'the Graduate College, the University of Vermont, in partial fulfillment of the requirement for the degree of Doctor of Philosophy specializing in Biology. Dissertation Examination Committee: Advisor LS,, ~inU0.Vigoreaux, Ph.D. (? &A- Chairperson George C. ellman, P1i.D. Associate Dean, Graduate College Patricia A. Sloko\~sl<i,P1i.D. Date: July 27. 2009 ABSTRACT Ca2+ influx through ionotropic glutamate receptors regulates a variety of developmental processes including neurite outgrowth and naturally occurring cell death. In the CNS, NMDA receptors were originally thought to be the sole source of Ca2+ influx through glutamate receptors; however, AMPA receptors also allow a significant influx of Ca2+ ions. The Ca2+ permeability of AMPA receptors is regulated by the insertion of one or more edited GluR2 subunits into the receptors. Although Ca2+-permeable AMPA receptors are a familiar feature in developing neurons, the developmental function of these receptors during the formation of the nervous system has yet to be established. This study was designed to investigate the expression and functional role of Ca2+-permeable AMPA receptors in developing chicken spinal motoneurons. Our results demonstrate that chicken lumbar motoneurons express functional AMPA receptors as early as embryonic day (E) 5. Electrophysiological recordings of kainate-evoked currents indicate a significant reduction in the Ca2+ permeability of AMPA receptors between E6 and E11. During this developmental period, the Ca2+ permeability of AMPA receptors decreases three-fold. Reduction in the Ca2+ permeability of AMPA receptors is accompanied by increased expression of GluR2 mRNA in the spinal motoneuron pool. Changes in GluR2 mRNA expression occur in parallel to changes in GluR2 protein expression in the chicken ventral spinal cord. Changes in the Ca2+-permeability of AMPA receptors are not mediated by age-dependent changes in the editing pattern of GluR2 subunits. At early stages of development, functional AMPA receptors were composed of a combination of GluR3 and GluR4 subunits. mRNA analysis indicates that GluR4 is the most abundant subunit in the chicken ventral spinal cord between E6 and E11. Immunohistochemistry analysis of spinal cord sections also demonstrated that both GluR3 and GluR4 proteins are expressed at E6 and E11. Expression of Ca2+-permeable AMPA receptors regulates the maturation of dendritic outgrowth in developing spinal motoneurons. Measurements of dendritic length and branching pattern demonstrate significant changes in the dendritic morphology of spinal motoneurons between E6 and E11. Blockade of AMPA receptor activation with CNQX between E5 and E8 causes a significant increase in dendritic outgrowth in lumbar motoneurons, when compared with vehicle-treated embryos. Treatment of chicken embryos with CNQX between E8 and E11, when AMPA receptors become Ca2+- impermeable, has no affect on dendritic morphology. However, blockade of NMDA receptor activation with MK-801 causes a significant reduction in dendritic outgrowth of lumbar motoneurons by E11. These findings indicate that AMPA receptor activation between E5 and E8 limits dendritic outgrowth in developing motoneurons, whereas NMDA receptor activation is involved in dendritic remodeling after the establishment of synaptic contacts with sensory afferents. CITATIONS Chapter 2 from this dissertation has been published in the following form: Ni, X., Sullivan, G.J., Martin-Caraballo, M.. (2007) Developmental characteristics of AMPA receptors in chick lumbar motoneurons. Developmental Neurobiology 67:1419-1432. Chapter 4 from this dissertation has been submitted for publication to Journal of Comparative Neurology on May, 15, 2009 in the following form: Ni, X., Martin-Caraballo, M.. Differential effect of glutamate receptor blockade on dendritic outgrowth in lumbar motoneurons. Journal of Comparative Neurology. ii ACKNOWLEDGEMENTS Here, I will thank all the people who have helped and encouraged me to finish my graduate study at the University of Vermont. First of all, thank Dr. Miguel Martin-Caraballo, my advisor for providing all the academic, financial and moral support for my work. His dedication towards work and his honesty in treating graduate students are absolutely unforgettable, which will have a positive influence on my future work. Second, my committee members Dr. George C. Wellman, Dr. Jim O. Vigoreaux and Dr. Karen M. Lounsbury for their advice and encouragement during the past few years. Thomm Buttolph, Edward Zelazny, Dr. Sheryl White for their enthusiasm and dedication to technical training and support. Yimin Liu for encouraging and guiding me to get used to the new environment and helping me solve technical problems. Andrew Shepherd for helpful comments on an early draft of my dissertation and helping me to correct grammar mistakes. Donna Panko, Elaine Morse and Norma Miller for helping and encouraging me to face all the difficulties. My labmates Deblina Dey, Yoon Yone Jung and Alexander Gokin for their help and bringing friendly atmosphere while working in the lab. All my friends in Vermont, who brought me joy while living far away from my family. Lastly, my parents and my dear sister who always care about me and encourage me to realize my goals in life. To my fiancé for his trust and endless support. iii This research project was supported by the Center of Biomedical Research Excellence (COBRE) in Neuroscience at the University of Vermont. iv TABLE OF CONTENTS Page CITATIONS………………………………………………………………………………ii ACKNOWLEDGEMENTS………………………………………………………..……..iii LIST OF FIGURES……………………………………………………………..............viii CHAPTER 1 INTRODUCTION……………………………………………………………………..….1 1. Glutamate receptors: a general Overview……………………………………...……..1 2. Posttranscriptional modifications of AMPA receptors………………………….........6 3. Role of GluR2 subunit in regulating AMPA receptor properties……………….........8 4. AMPA receptor function……………………………………………………..……...11 5. Developmental regulation of AMPA receptors……………………………….…….13 6. Regulation of AMPA receptor function by electrical activity………………..……..16 7. Regulation of AMPA receptor function by trophic factors……………………........18 8. The avian spinal cord has many advantages for cellular studies of neuronal development…………………………………………………………………….……...20 9. Generation of spontaneous electrical activity is an early feature of spinal cord development…………………………………………………………………………....26 10. Role of cell death in spinal cord development…………………………………......27 11. Morphological maturation of spinal motoneurons…………………………...........29 12. Rationale for studying the developmental regulation of AMPA receptors in spinal motoneurons.……………………………………………………………………….......32 Figures………………………………………………………….....................................35 References………………………………………………………………………...........38 v CHAPTER 2 Developmental Characteristics of AMPA Receptors in Chick Lumbar Motoneurons…………………………………………………………………………...52 Abstract……………………………………………………………………………..….53 Introduction………………………………………………………………………..…...54 Methods………………………………………………………………………………...57 Results……………………………………………………………………………….....65 Discussion……………………………………………………………………………...71 References……………………………………………………………………………...81 Figures……………………………………………………………………………….....87 CHAPTER 3 Subunit composition of AMPA receptors in the chicken spinal cord during early Embryonic development…………………….………………………………………....97 Abstract………………………………………………………………………………...98 Introduction..……………………………………………………………………...........99 Methods……………………………………………………………………………….102 Results………………………………………………………………………………...108 Discussion…..………………………………………………………………………...112 References……..……………………………………………………………………...116 Figures…………………………………………………………..………………….....119 CHAPTER 4 Differential effect of glutamate receptor blockade on dendritic outgrowth in lumbar Motoneurons……….………………………………………………………………….125 Abstract…………………………………………………………………..…………...127 vi Introduction…………………………………………………………………...............128 Methods…..…………………………………………………………………………...131 Results………………………………………………..……………………………….136 Discussion…………………………………..………………………………………...145 References………………..…………………………………………………………...152 Figures………..………………………………………………………………….........156 CHAPTER 5 General discussion and future aims………………..……………………………........173 1. General discussion……………………………………………………..……..…..173 2. Conclusions…………………………………………..…………………………...181
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  • Oxford Handbooks Online (

    Oxford Handbooks Online (

    AMPA and Kainate Receptors AMPA and Kainate Receptors G. Brent Dawe, Patricia M. G. E. Brown, and Derek Bowie The Oxford Handbook of Neuronal Ion Channels Edited by Arin Bhattacharjee Subject: Neuroscience, Molecular and Cellular Systems Online Publication Date: Oct 2020 DOI: 10.1093/oxfordhb/9780190669164.013.8 Abstract and Keywords α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and kainate-type gluta­ mate receptors (AMPARs and KARs) are dynamic ion channel proteins that govern neu­ ronal excitation and signal transduction in the mammalian brain. The four AMPAR and five KAR subunits can heteromerize with other subfamily members to create several com­ binations of tetrameric channels with unique physiological and pharmacological proper­ ties. While both receptor classes are noted for their rapid, millisecond-scale channel gat­ ing in response to agonist binding, the intricate structural rearrangements underlying their function have only recently been elucidated. This chapter begins with a review of AMPAR and KAR nomenclature, topology, and rules of assembly. Subsequently, receptor gating properties are outlined for both single-channel and synaptic contexts. The struc­ tural biology of AMPAR and KAR proteins is also discussed at length, with particular fo­ cus on the ligand-binding domain, where allosteric regulation and alternative splicing work together to dictate gating behavior. Toward the end of the chapter there is an overview of several classes of auxiliary subunits, notably transmembrane AMPAR regula­ tory proteins and Neto proteins, which enhance native AMPAR and KAR expression and channel gating, respectively. Whether bringing an ion channel novice up to speed with glutamate receptor theory and terminology or providing a refresher for more seasoned biophysicists, there is much to appreciate in this summation of work from the glutamate receptor field.