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Pierce's Disease Research Progress Reports for 2015

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Pierce's Disease Research Progress Reports for 2015 Research Progress Reports Pierce’s Disease and Other Designated Pests and Diseases of Winegrapes - December 2015 - Compiled by: Pierce’s Disease Control Program California Department of Food and Agriculture Sacramento, CA 95814 Chief Editor: Thomas Esser, CDFA Assistant Editor: Raj Randhawa, CDFA Cover Design and Photograph: Sean Veling, CDFA Cite as: Research Progress Reports: Pierce’s Disease and Other Designated Pests and Diseases of Winegrapes. December 2015. California Department of Food and Agriculture, Sacramento, CA. Available on the Internet at: http://www.cdfa.ca.gov/pdcp/Research.html Acknowledgements: Many thanks to the scientists and cooperators who are conducting research on Pierce’s disease and other designated pests and disease of winegrapes, and submitted reports for inclusion in this document. Note to Readers: The reports contained in this document have not been subject to scientific peer review. TABLE OF CONTENTS Section 1: Pierce’s Disease and Its Vectors REPORTS Assessing Pierce’s Disease Spread in Grape Lines with Novel Defensive Traits Rodrigo Almeida .............................................................................................................................................. 3 Evaluating Potential Shifts in Pierce’s Disease Epidemiology Rodrigo Almeida .............................................................................................................................................. 5 Exploiting a Chitinase to Suppress Xylella fastidiosa Colonization of Plants and Insects Rodrigo Almeida .............................................................................................................................................. 7 Identification of a New Virulence Factor Required for Pierce’s Disease and Its Utility in Development of a Biological Control Thomas J. Burr, Patricia Mowery, Luciana Cursino, and Lingyun Hao .......................................................... 9 Chimeric Antimicrobial Protein and Polygalacturonase-Inhibiting Protein Transgenic Grapevine Field Trial Abhaya M. Dandekar ..................................................................................................................................... 18 Defining the Role of Secreted Virulence Proteins LesA and PrtA in the Pathobiology of Xylella and in the Development of Pierce’s Disease Abhaya M. Dandekar ..................................................................................................................................... 27 The Riverside County Glassy-winged Sharpshooter Program in the Temecula Valley Matt Daugherty and Diane Soto ..................................................................................................................... 36 RNA-Interference and Control of the Glassy-winged Sharpshooter and Other Leafhopper Vectors of Xylella fastidiosa Bryce W. Falk ................................................................................................................................................. 40 High-Throughput Live Cell Screen for Small Molecules Targeting the TolC Efflux Pump of Xylella fastidiosa Dean W. Gabriel ............................................................................................................................................. 45 Field Evaluation of Grape Plants Expressing Potential Protective DNA Sequences Effective Against Pierce’s Disease David G. Gilchrist and James E. Lincoln ....................................................................................................... 49 Field Evaluation of Grape Plants Expressing PR1 and UT456 Transgenic DNA Sequences for Protection Against Pierce’s Disease David G. Gilchrist and James E. Lincoln ....................................................................................................... 54 Transgenic Rootstock-Mediated Protection of Grapevine Scions By Single and Stacked DNA Constructs David G. Gilchrist, James E. Lincoln, Abhaya M. Dandekar, and Steven Lindow ....................................... 59 - i - Conducting Pierce’s Disease Symptom Evaluations at the Solano County Field Trial Research Block Deborah A. Golino ......................................................................................................................................... 67 Biological Control of Pierce’s Disease of Grapevine in the UC Riverside Vineyard with a Benign Strain of Xylella fastidiosa Donald Hopkins .............................................................................................................................................. 72 Selective Disruption of Glassy-winged Sharpshooter Maturation and Reproduction by RNA- Interference Shizuo George Kamita ................................................................................................................................... 77 Characterization of the Xylella fastidiosa PhoP/Q and ColR/ColS Two-Component Regulatory System Bruce Kirkpatrick ........................................................................................................................................... 84 Evaluation of Pierce’s Disease Resistance in Transgenic Vitis vinifera Grapevines Expressing Xylella fastidiosa Hemagglutinin Protein Bruce Kirkpatrick ........................................................................................................................................... 93 Substrate-Borne Vibrational Signals in Intraspecific Communication of the Glassy-winged Sharpshooter Rodrigo Krugner and Valerio Mazzoni ........................................................................................................ 104 Comparison and Optimization of Different Methods to Alter Diffusible Signal Factor Mediated Signaling in Xylella fastidiosa in Plants to Achieve Pierce’s Disease Control Steven Lindow .............................................................................................................................................. 110 Continued Field Evaluation of Diffusible Signal Factor Producing Grape for Control of Pierce’s Disease Steven Lindow .............................................................................................................................................. 127 Monitoring for Insecticide Resistance in the Glassy-winged Sharpshooter Thomas M. Perring and Nilima Prabhaker ................................................................................................... 142 Field Evaluations of Grafted Grape Lines Expressing Polygalacturonase-Inhibiting Proteins Ann L.T. Powell and John M. Labavitch ..................................................................................................... 147 Management of Insecticide Resistance in Glassy-winged Sharpshooter Populations Using Toxicological, Biochemical, and Genomic Tools Richard Redak, Bradley White, and Frank Byrne ........................................................................................ 157 Genome Editing of TAS4, MIR828, and TargetsMY BA6/A7: A Critical Test of Xylella fastidiosa Infection and Spreading Mechanisms in Pierce’s Disease Chris Rock, Leo De La Fuente, Sunitha Sukumaran, and Sy Mamadou Traore .......................................... 164 Continuation of the Field Evaluation of New Strategies for the Management of Pierce’s Disease of Grapevine Philippe Rolshausen, Matt Daugherty, and Peggy Mauk ............................................................................. 171 Evaluation of Natural Products Isolated from Grapevine Fungal Endophytes for Control of Pierce’s Disease Philippe Rolshausen, Caroline Roper, and Katherine Maloney ...................................................................181 - ii - Characterization and Inhibition of Xylella fastidiosa Proteins Secreted by the Type II Secretion System and Their Secretion Machinery Caroline Roper ............................................................................................................................................. 188 Characterization of the Lipopolysaccharide-Mediated Response to Xylella fastidiosa Infection in Grapevine Caroline Roper and Dario Cantu .................................................................................................................. 201 Development of a Grape Tissue Culture and Transformation Platform for the California Grape Research Community David M. Tricoli ........................................................................................................................................... 213 Expanding the Range of Grape Rootstock and Scion Genotypes That Can Be Genetically Modified for Use in Research and Product Development David M. Tricoli ........................................................................................................................................... 223 Breeding Pierce’s Disease Resistant Winegrapes Andrew Walker ............................................................................................................................................ 231 Molecular Breeding Support for the Development of Pierce’s Disease Resistant Winegrapes Andrew Walker and Dario Cantu ................................................................................................................. 240 ABSTRACTS Stable Plasmid Vectors for Complementation of Xylella fastidiosa Mutants in Planta Lindsey P. Burbank and Drake C. Stenger ................................................................................................... 248 Whole Genome Sequence Analyses of Xylella fastidiosa Pierce’s Disease
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