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P. Gopalakrishnakone Editor-In-Chief Anita Malhotra Editor Evolution of Venomous Animals and Their Toxins Toxinology Toxinology P. Gopalakrishnakone Editor-in-Chief Anita Malhotra Editor Evolution of Venomous Animals and Their Toxins Toxinology Editor-in-Chief P. Gopalakrishnakone In recent years, the field of toxinology has expanded substantially. On the one hand it studies venomous animals, plants and micro organisms in detail to understand their mode of action on targets. On the other hand, it explores the biochemical compo- sition, genomics and proteomics of toxins and venoms to understand their three interaction with life forms (especially humans), development of antidotes and exploring their pharmacological potential. Therefore, toxinology has deep linkages with biochemistry, molecular biology, anatomy and pharmacology. In addition, there is a fast-developing applied subfield, clinical toxinology, which deals with under- standing and managing medical effects of toxins on human body. Given the huge impact of toxin-based deaths globally, and the potential of venom in generation of drugs for so-far incurable diseases (for example, diabetes, chronic pain), the contin- ued research and growth of the field is imminent. This has led to the growth of research in the area and the consequent scholarly output by way of publications in journals and books. Despite this ever-growing body of literature within biomedical sciences, there is still no all-inclusive reference work available that collects all of the important biochemical, biomedical and clinical insights relating to toxinology. Composed of 12 volumes, Toxinology provides comprehensive and authoritative coverage of the main areas in toxinology, from fundamental concepts to new developments and applications in the field. Each volume comprises a focused and carefully chosen collection of contributions from leading names in the subject. Series Titles 1. Biological Toxins and Bioterrorism 2. Clinical Toxinology in the Asia Pacific and Africa 3. Spider Venoms 4. Scorpion Venoms 5. Marine and Freshwater Toxins 6. Venom Genomics and Proteomics 7. Snake Venoms 8. Evolution of Venomous Animals and Their Venoms 9. Microbial Toxins 10. Plant Toxins 11. Toxins and Drug Discovery 12. Clinical Toxinology in Australia, Europe, and Americas More information about this series at http://www.springer.com/series/13330 P. Gopalakrishnakone Editor-in-Chief Anita Malhotra Editor Evolution of Venomous Animals and Their Toxins With 89 Figures and 10 Tables Editor-in-Chief P. Gopalakrishnakone Venom and Toxin Research Programme Department of Anatomy Yong Loo Lin School of Medicine National University of Singapore Singapore, Singapore Editor Anita Malhotra School of Biological Sciences Bangor University Bangor, Gwynedd, UK ISBN 978-94-007-6457-6 ISBN 978-94-007-6458-3 (eBook) ISBN 978-94-007-6459-0 (print and electronic bundle) DOI 10.1007/978-94-007-6458-3 Library of Congress Control Number: 2016962206 # Springer Science+Business Media Dordrecht 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Springer imprint is published by Springer Nature The registered company is Springer Science+Business Media B.V. The registered company address is: Van Godewijckstraat 30, 3311 GX Dordrecht, The Netherlands Series Preface The term TOXIN is derived from the Greek word Toeikov and is defined as a substance derived from tissues of a plant, animal, or microorganism that has a deleterious effect on other living organisms. Studying their detailed structure, function, and mechanism of action as well as finding an antidote to these toxins is the field of TOXINOLOGY, and the scientists are called TOXINOLOGISTS. In recent years, the field of toxinology has expanded substantially. On the one hand, it studies venomous animals, plants, and microorganisms in detail to under- stand their habitat, distribution, identification, as well as mode of action on targets, while on the other, it explores the biochemical composition, genomics, and proteo- mics of toxins and venoms to understand their interaction with life forms (especially humans), the development of antidotes, and their pharmacological potential for drug discovery. Therefore, toxinology has deep linkages with biochemistry, molecular biology, anatomy, pharmacology, etc. In addition, there is a fast developing applied subfield, clinical toxinology, which deals with understanding and managing medical effects of venoms and toxins on the human body following envenomations. Given the huge impact of envenomation-based deaths globally and the potential of venom in the generation of drugs for debilitating diseases (e.g., diabetes, chronic pain, and cancer), the continued research and growth of the field is imminent. Springer has taken the bold initiative of producing this series, which is not an easy target of producing about 12 volumes, namely, biological toxins and bioterrorism, clinical toxinology, scorpion venoms, spider venoms, snake venoms, marine and freshwater toxins, toxins and drug discovery, venom genomics and proteomics, evolution of venomous animals and their toxins, plant toxins, and microbial toxins. Singapore P. Gopalakrishnakone M.B.B.S., Ph.D., F.A.M.S., D.Sc. Editor-in-Chief v Acknowledgments I would like to sincerely thank the section editor of this volume, Anita Malhotra, for the invaluable contribution of her expertise and time and the authors who obliged with my request and provided a comprehensive review on the topics. Springer provided substantial technical and administrative help by many individ- uals at varying levels, but special mention should go to Sarah Mathews, Sunali Mull, Meghna Singh, Mokshika Gaur, and Audrey Wong for their tireless effort in bringing these volumes to reality. Singapore P. Gopalakrishnakone M.B.B.S., Ph.D., F.A.M.S., D.Sc. Editor-in-Chief vii Volume Preface Thomas Dobzhansky famously wrote “Nothing in Biology Makes Sense Except in the Light of Evolution” in his 1937 essay, a statement that firmly established evolution as a unifying concept in biology. To put it in another way, while classical toxinology investigated the “what” and “how” questions about animal toxins, the “why” questions involving the wider evolutionary context in which venomous animals developed was often overlooked. However, I am glad to say that this is no longer the case, and recent years have seen a free exchange of ideas between toxinologists and evolutionary biologists, with benefits to each field. Thus, this volume opens with a section containing chapters on the wider evolutionary context of venom in animals, the molecular evolu- tionary processes involved in generating diversity, and the concept of venom evolution as being driven by an arms race that also involves evolution of resistance to toxins by prey. As an evolutionary biologist, I am also strongly convinced that we can only study the evolution of toxins if it is underpinned by a clear understanding of the evolution of the animals that they evolved within. The original concept of the book was that a chapter on the evolution of venom and toxins within a particular group of venomous animals (Sect. 2) would be balanced by a chapter giving the latest understanding of the systematics of that group (Sect. 4). Our understanding of relationships changes all the time as new data or new methods of analysis become available, and the Handbook of Toxinology format is eminently suited to frequent updating. Finally, the volume finishes with a section of the evolution of venom delivery systems. The definition of a venomous animal, as opposed to a poisonous one, encompasses the evolution not just of toxins but also a specialized mechanism for administering them by injection. While venom delivery systems have been well studied in some groups, such as snakes, we are only just beginning to learn about the origins and amplification of these systems in other groups, such as centipedes. I am deeply grateful to the many authors who contributed to this volume for agreeing to share their expertise. I also wish to express my thanks to Professor Ponnampalam Gopalakrishnakone for inviting me to edit this volume and to the professional and patient editorial team at Springer (particularly Audrey Wong, Sunali Mull, and Sarah Mathews) for their support during the process. October 2016 Anita Malhotra Bangor, Gwynedd, UK B.A. (Oxon), Ph.D., F.L.S, F.R.S.B., F.Z.S.L ix Contents Part I The Wider Evolutionary Context ....................... 1 1 Evolutionary Context of Venom
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