Russian Entomol. J. 29(1): 20–32 © RUSSIAN ENTOMOLOGICAL JOURNAL, 2020 An illustrated taxonomic key to genera of Mirinae (Hemiptera: Heteroptera: Miridae) with three new records from Iran Èëëþñòðèðîâàííûé îïðåäåëèòåëü ðîäîâ Mirinae (Hemiptera: Heteroptera: Miridae) è òðè íîâûõ íàõîäêè äëÿ Èðàíà Mozhgan Zamani, Reza Hosseini* Ì. Çàìàíè, Ð. Õîññåéíè Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran. P.O. Box: 41635–1314. E–mail: [email protected], [email protected] *corresponding author KEY WORDS: Identification key, female genitalia, New record, Mirinae, Miridae, Iran. КЛЮЧЕВЫЕ СЛОВА. Определитель, гениталии самок, новые находки, Mirinae, Miridae, Иран. ABSTRACT. A comprehensive identification key to be phytophagous [Schuh, Slater, 1995; Wheeler, 2001]. has been compiled for 35 genera belonging to three Mirinae subfamily has been categorized into six tribes of the Mirinae subfamily found in Iran. Illustra- tribes including Herdoniini, Hyalopeplini, Mecistosce- tion of male and female genitalia for most genera, lini, Mirini, Resthenini, and Stenodemini [Schwartz, digital images of habitus and other diagnostic charac- 2008; Cassis, Schuh, 2012]. Three of them, namely ters were provided to aid unequivocal identification. Herdoniini, Mirini and Stenodemini have been reported Rauniella ishtar (Linnavuori, 1984), Cyphodema from Iran [Ghahari, Cherot, 2014]. These tribes respec- humbaba Linnavuori, 1984, and Stenotus binotatus tively comprise with 38, 250 and 34 genera in the world (Fabricius, 1794) are briefly redescribed and reported [Schuh, 2002–2013], where about 1, 27 and 6 genera as three new species for Iranian fauna. found and documented from Iran [Aukema, Rieger, 1999; Aukema et al., 2013]. Validity of report for Cypho- РЕЗЮМЕ. Составлен определитель для 35 родов, dema mendosa Montandon, 1887 is in doubt and it is принадлежащих к трем трибам подсемейства Mirinae, unclear if vouchers were deposited for further examina- известных из Ирана. Приведены изображения габи- tion. The genus Hadrodemus was also erroneously recor- туса, мужских и женских гениталий и других диагно- ded from Iranian fauna [Aukema et al., 2013]. стических признаков для большинства родов, позво- Although the Mirinae is one of the best studied sub- ляющие однозначно идентифицировать эти роды. families of Miridae in the world from a taxonomic per- Rauniella ishtar (Linnavuori, 1984), Cyphodema spective, there are few valid keys designed specially to humbaba Linnavuori, 1984 и Stenotus binotatus aid the identification of genera in this group [Carvalho, (Fabricius, 1794) кратко переописаны и указаны как 1955; Kerzhner, 1964; Wagner, Weber, 1964; Wagner, новые виды для фауны Ирана. 1974; Rosenzweig, 2001; Schwartz, 2008]. So far only a few taxonomic works have been done on the subfamily in Introduction Iran [e.g., Linnavouri, 1999, 2006, 2009; Hosseini, 2014, 2015, 2016] and no appropriate taxonomic key has been compiled for identification of Iranian genera. Miridae or plant bugs are the largest family of Hemi- During spring–summer of 2018 we conducted a ptera, with more than 11020 described species in the field investigation in mountainous regions and deep world [Cassis, Schuh, 2012]. This family comprises sev- valleys in Guilan, Mazandaran and western part of en subfamilies including Deraeocorinae, Mirinae, Ortho- Kurdistan provinces. Examination of sampled material tylinae, Cylapinae, Bryocorinae, Isometopinae, and Phyli- resulted in finding of three species new to Iranian fauna nae. Among them, Mirinae with at least 4100 species in which are briefly redescribed below. approximately 350 genera is considered to be one of the We also compiled a comprehensive taxonomic key most diverse and complex group of Miridae. Most spe- for identification of all Mirinae genera currently known cies inhabit on grasses and herbaceous plants and appear from Iran. Illustration of male and female genitalia of How to cite this article: Zamani M., Hosseini R. 2020. An illustrated taxonomic key to genera of Mirinae (Hemiptera: Heteroptera: Miridae) with three new records from Iran // Russian Entomol. J. Vol.29. No.1. P.20 – 32. doi: 10.15298/rusentj.29.1.04 An illustrated taxonomic key to genera of Mirinae from Iran 21 most of genera, digital images of habitus and diagnostic 2nd segment #/$: 1.6x/ 1.4–1.5x as long as width of head; characters for new record species are provided to aid length of pronotum (dorsal view) #/$: 0.96–0.98/ 0.94– unequivocal identification. 1.07; width of posterior margin of pronotum #/$: 1.7/1.83. MALE GENITALIA. Left paramere sickle-shaped, later- al side of sensory lobe with setiferous tubercles transformed Materials and methods into distinct teeth (Fig. 8); right paramere broad and short, basal margin dentate, ventrally with small setiferous tuber- cles (Fig. 9); vesica with several membranous lobes, a single In an ongoing faunal study in Iran, mirid specimens spiculum and a small spinulated plate near secondary gonop- were collected by sweeping during spring–summer 2018 ore, one of the main membranous lobes laterally with small from Guilan, Mazandaran and also the western part of dentate field, another one dorsally dentate and sclerotized, Kurdistan provinces. Authentically identified species secondary gonopore large and oval (Fig. 7). available in the insect collection of the Natural History FEMALE GENITALIA. Dorsal labiate plate wide, lateral- Museum of the University of Guilan (UGNHM) were ly prominent, with pointed and reinforced margins; sclerotized used for compiling generic taxonomic key, although, rings small, oval, distinctly separated, placed laterally, ob- Capsus, Pantilius and Rhabdomiris were not available liquely oriented; posterior wall with large interramal sclerites for examination. Adult specimens were examined using (medially fused), small and spinose interramal lobes (medially separated), broadly rounded, disk–shaped and spinose dorsal a stereomicroscope (GX Microscope, Australia). All structure and prominent medial process (Fig. 81). measurements were made with ocular micrometer and COMMENTS. Nine species of Cyphodema have been are given in millimeters. Dissections of male genitalia described in the world [Schuh, 2002–2013], and Cyphodema were performed using the technique described by Scud- humbaba is the first record from Iran. Collected on herba- der and Schwartz [2012]. The male and female genitalia ceous host plants in meadow. were photographed with a Canon EOS 600D DSLR Rauniella Rosenzweig, 1997 camera attached to Leica DM 1000 microscope and illustrated using a drawing tube attached to an BH-2 Rauniella ishtar (Linnavuori, 1984) Olympus microscope. Color images of the adult dry- Figs 5–6, 10–13, 89. mounted specimens were captured using a hand-made SPECIMENS EXAMINED. Iran, Kurdistan province: Sarva- automated digital imaging system composed of a Canon bad; Daraki 5#, 1$ (35°19´N 46°09´E, 1831m), Bahram abad EOS 70D DSLR camera, Canon EF 100 mm f/2.8 USM (35°21´N 46°14´E, 1183m) S. Mohammadi, sweeping. 15.VI.2018. DIAGNOSIS. Rauniella ishtar can be distinguished by Macro lens and a 65 mm Meike macro extension tube. the following characters: interocular width/width of eye #/$: Partially focused images (more than 65) were combined 1.4–1.57/ 1.73; pronotum laterally below the humeral angles using Helicon Focus image stacking software (ver. 6) with black markings; femora with rows of dark brown spots; (http://www.heliconsoft.com). Images were edited us- tibia with black spines; lateral side of left paramere subapi- ing Adobe Photoshop CC 2017. Newly collected and cally with a large dentate projection; right paramere medially examined specimens are deposited in the insect collec- constricted; vesica without spiculum; secondary gonopore tion of the Natural History Museum of the University of forming an inverted saddle; sclerotized rings of female geni- Guilan (UGNHM), Rasht, Iran. Terminology of the talia large, elongate-oval; dorsal labiate plate with a large male and female genitalia follows Konstantinov [2003] antero-medial projection; median process of posterior wall and Schwartz [2008] respectively. prominent. MEASUREMENTS. Body length #/$: 7.75–8.15/ 7.89; width #/$: 2.41/ 2.62; interocular width/width of eye #/$: Taxonomy 1.4–1.57/ 1.73; lengths of antennal segments I–IV#/$: 1.16– 1.23, 2.86–3.16, 1.83–1.94, ?/ 1.07, 2.98, 1.6, ?; 2nd segment Cyphodema Fieber, 1858 #/$: 2.4–2.7x/ 2.3x longer than width of head; length of pronotum (dorsal view) #/$: 1.19/ 1.37; width of posterior Cyphodema humbaba Linnavuori, 1984 margin of pronotum #/$: 2–2.15/ 2.36. Figs 3–4, 7–9, 81. MALE GENITALIA. Apophysis of left paramere form- SPECIMENS EXAMINED. Iran, Kurdistan province: Sarva- ing almost right angle with its body, with large subapical bad; Daraki 2#, 2$ (35°19´N 46°09´E, 1831m), S. Mohammadi, dentate projection on lateral side, apically curved sideward, sweeping. 15.VI.2018. sensory lobe and lateral margin of left paramere with setifer- DIAGNOSIS. Cyphodema humbaba is recognized by the ous tubercles transformed into distinct teeth (Figs 11–12); following characters: interocular width/width of eye #/$: right paramere medially constricted (Fig. 13); vesica with 1.38–1.5/ 1.6; labium reaching or slightly surpassing meta- three main large lobes, without spiculum; one of the main coxa; clavus blackish, posteriorly stramineous; corium posteri- lobes dentate and sclerotized; other two main lobes, membra- orly with large blackish marking; tibia with golden spines; nous and