YWHAG Antibody Cat. No.: 61-614

YWHAG Antibody

Formalin-fixed and paraffin-embedded human colon Western blot analysis of YWHAG Antibody in mouse carcinoma reacted with YWHAG Antibody , which was cerebellum tissue lysates (35ug/lane) peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of widr cells using YWHAG Western blot analysis of YWHAG using rabbit polyclonal Antibody (bottom histogram) compared to a negative YWHAG Antibody using 293 cell lysates (2 ug/lane) either control cell (top histogram)FITC-conjugated goat-anti- nontransfected (Lane 1) or transiently transfected (Lane 2) rabbit secondary antibodies were used for the analysis. with the YWHAG .

Specifications

September 26, 2021 1 https://www.prosci-inc.com/ywhag-antibody-61-614.html HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse

Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Rat, HOMOLOGY: Xenopus

This YWHAG antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 136-164 amino acids from the Central region of human YWHAG.

TESTED APPLICATIONS: Flow, IHC-P, WB

For WB starting dilution is: 1:1000

APPLICATIONS: For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

PREDICTED MOLECULAR 28 kDa WEIGHT:

Properties

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by PURIFICATION: dialysis

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: YWHAG

14-3-3 gamma, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein gamma, ALTERNATE NAMES: N-terminally processed, YWHAG

ACCESSION NO.: P61981

PROTEIN GI NO.: 48428721

GENE ID: 7532

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

September 26, 2021 2 https://www.prosci-inc.com/ywhag-antibody-61-614.html Background and References

YWHAG belongs to the 14-3-3 family of which mediate by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. BACKGROUND: It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

REFERENCES: 1) Jagemann,L.R., et.al., J. Biol. Chem. 283 (25), 17450-17462 (2008)

ANTIBODIES FOR RESEARCH USE ONLY.

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September 26, 2021 3 https://www.prosci-inc.com/ywhag-antibody-61-614.html