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Analysis of Drugs Manual September 2019
Drug Enforcement Administration Office of Forensic Sciences Analysis of Drugs Manual September 2019 Date Posted: 10/23/2019 Analysis of Drugs Manual Revision: 4 Issue Date: September 5, 2019 Effective Date: September 9, 2019 Approved By: Nelson A. Santos Table of Contents CHAPTER 1 – QUALITY ASSURANCE ......................................................................... 3 CHAPTER 2 – EVIDENCE ANALYSIS ......................................................................... 93 CHAPTER 3 – FIELD ASSISTANCE .......................................................................... 165 CHAPTER 4 – FINGERPRINT AND SPECIAL PROGRAMS ..................................... 179 Appendix 1A – Definitions ........................................................................................... 202 Appendix 1B – Acronyms and Abbreviations .............................................................. 211 Appendix 1C – Instrument Maintenance Schedule ..................................................... 218 Appendix 1D – Color Test Reagent Preparation and Procedures ............................... 224 Appendix 1E – Crystal and Precipitate Test Reagent Preparation and Procedures .... 241 Appendix 1F – Thin Layer Chromatography................................................................ 250 Appendix 1G – Qualitative Method Modifications ........................................................ 254 Appendix 1H – Analytical Supplies and Services ........................................................ 256 Appendix 2A – Random Sampling Procedures -
The Interpersonal Effects of Pride and Shame Expressions on Opponents and Teammates
ORIGINAL RESEARCH published: 08 September 2015 doi: 10.3389/fpsyg.2015.01361 “Put your Hands up in the Air”? The interpersonal effects of pride and shame expressions on opponents and teammates Philip Furley 1*, Tjerk Moll 2 and Daniel Memmert 1 1 Institute of Cognitive and Team/Racket Sport Research, German Sport University Cologne, Cologne, Germany, 2 Cardiff School of Sport, Cardiff Metropolitan University, Cardiff, UK The aim of the present research was to investigate the interpersonal effects of pride and shame expressions amongst opponents and teammates in a soccer penalty scenario. Across a series of experiments using the point-light method, pride and shame expressions exerted strong effects upon observers’ anticipated emotions, associated cognitions, and performance expectations. Using the Implicit Association Test (IAT) in two pilot studies we demonstrated that the created pride and shame point-light stimuli were implicitly associated with status and performance related attributes. In Edited by: Experiment 1, observing pride expressions caused opponents to anticipate more Gerben A. Van Kleef, University of Amsterdam, Netherlands negative emotions, cognitions, and lower performance expectancies toward their next Reviewed by: performance in comparison with neutral expressions. In contrast, pride expressions led Jozefien De Leersnyder, teammates to anticipate more positive emotions (i.e., pride and happiness), cognitions, University of Leuven, Belgium Marc W. Heerdink, and performance expectations toward their next performance than neutral expressions University of Amsterdam, Netherlands (Experiments 2–4). The results are discussed within the emotions as social information *Correspondence: (EASI, Van Kleef, 2009) framework by arguing that the social context has to be taken Philip Furley, into account when investigating the interpersonal effects of emotion expressions. -
Cannabigerol Is a Potential Therapeutic Agent in a Novel Combined Therapy for Glioblastoma
cells Article Cannabigerol Is a Potential Therapeutic Agent in a Novel Combined Therapy for Glioblastoma Tamara T. Lah 1,2,3,*, Metka Novak 1, Milagros A. Pena Almidon 4, Oliviero Marinelli 4 , Barbara Žvar Baškoviˇc 1, Bernarda Majc 1,3, Mateja Mlinar 1, Roman Bošnjak 5, Barbara Breznik 1 , Roby Zomer 6 and Massimo Nabissi 4 1 Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, 1000 Ljubljana, Slovenia; [email protected] (M.N.); [email protected] (B.Ž.B.); [email protected] (B.M.); [email protected] (M.M.); [email protected] (B.B.) 2 Faculty of Chemistry and Chemical Technology, University of Ljubljana, 1000 Ljubljana, Slovenia 3 Jožef Stefan International Postgraduate School, 1000 Ljubljana, Slovenia 4 School of Pharmacy, Experimental Medicine Section, University of Camerino, 62032 Camerino, Italy; [email protected] (M.A.P.A.); [email protected] (O.M.); [email protected] (M.N.) 5 Department of Neurosurgery, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia; [email protected] 6 MGC Pharmaceuticals d.o.o., 1000 Ljubljana, Slovenia; [email protected] * Correspondence: [email protected]; Tel.: +386-41-651-629 Simple Summary: Among primary brain tumours, glioblastoma is the most aggressive. As early relapses are unavoidable despite standard-of-care treatment, the cannabinoids delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD) alone or in combination have been suggested as a combined treatment strategy for glioblastomas. However, the known psychoactive effects of THC hamper its medical applications in these patients with potential cognitive impairment due to the progression of the Citation: Lah, T.T.; Novak, M.; Pena Almidon, M.A.; Marinelli, O.; disease. -
A Dissertation Entitled Uncovering Cannabinoid Signaling in C. Elegans
A Dissertation Entitled Uncovering Cannabinoid Signaling in C. elegans: A New Platform to Study the Effects of Medicinal Cannabis By Mitchell Duane Oakes Submitted to the Graduate Faculty as partial fulfillment of the requirements for the Doctor of Philosophy Degree in Biology ________________________________________ Dr. Richard Komuniecki, Committee Chair _______________________________________ Dr. Bruce Bamber, Committee Member ________________________________________ Dr. Patricia Komuniecki, Committee Member ________________________________________ Dr. Robert Steven, Committee Member ________________________________________ Dr. Ajith Karunarathne, Committee Member ________________________________________ Dr. Jianyang Du, Committee Member ________________________________________ Dr. Amanda Bryant-Friedrich, Dean College of Graduate Studies The University of Toledo August 2018 Copyright 2018, Mitchell Duane Oakes This document is copyrighted material. Under copyright law, no parts of this document may be reproduced without the expressed permission of the author. An Abstract of Uncovering Cannabinoid Signaling in C. elegans: A New Platform to Study the Effects of Medical Cannabis By Mitchell Duane Oakes Submitted to the Graduate Faculty as partial fulfillment of the requirements for the Doctor of Philosophy Degree in Biology The University of Toledo August 2018 Cannabis or marijuana, a popular recreational drug, alters sensory perception and exerts a range of medicinal benefits. The present study demonstrates that C. elegans exposed to -
High-Throughput Analysis of Cannabinoids by LC-UV
Featured Application: Cannabinoid Profiles on Raptor ARC-18 High-Throughput Analysis of Cannabinoids by LC-UV • Increase sample throughput with this fast, 9-minute analysis. • Baseline separation of 16 cannabinoids provides more accurate and comprehensive potency and profile data. • Simple, isocratic method is more easily transferable between instruments and labs than gradient methods. As the cannabis market grows, interest in more detailed analysis of cannabinoid profiles is expanding because more comprehensive data can be used for strain identification as well as to ensure more accurate potency testing. More than 100 cannabinoids have been isolated from cannabis to date, and these compounds can interfere with the five most commonly analyzed cannabinoids: tetrahydrocannabinol (THC), delta-9-tetra- hydrocannabinolic acid A (THCA), cannabidiol (CBD), cannabidiolic acid (CBDA), and cannabinol (CBN). The LC-UV method shown here uses a Raptor ARC-18 column to fully resolve 16 major and most frequently observed minor cannabinoids for which commercial standards are available. Baseline separation ensures positive identification and accurate quantitation. As shown, all compounds were resolved in a fast 9-minute analysis, making this method suitable for high-throughput cannabis testing labs. In addition, this analysis uses a simple isocratic mobile phase so it is more easily transferable between instruments, compared to more complex methods that incorporate atypical mobile phase gradients or additives. Peaks tR (min) Peaks tR (min) 1. Cannabidivarinic acid (CBDVA) 1.877 9. Cannabinol (CBN) 4.609 2. Cannabidivarin (CBDV) 2.086 10. Cannabinolic acid (CBNA) 5.437 3. Cannabidiolic acid (CBDA) 2.592 11. Δ9-Tetrahydrocannabinol (Δ9-THC) 5.815 4. Cannabigerolic acid (CBGA) 2.750 12. -
Friend to the Martyr, a Friend to the Woman of Shame': Thinking About the Law, Shame and Humiliation Michael L
digitalcommons.nyls.edu Faculty Scholarship Articles & Chapters 2014 'Friend to the Martyr, a Friend to the Woman of Shame': Thinking About the Law, Shame and Humiliation Michael L. Perlin New York Law School Naomi Weinstein Follow this and additional works at: https://digitalcommons.nyls.edu/fac_articles_chapters Part of the Human Rights Law Commons, Law and Psychology Commons, and the Law and Society Commons Recommended Citation 24 S. Cal. Rev. L. & Soc. Just. 1 2014-2015 This Article is brought to you for free and open access by the Faculty Scholarship at DigitalCommons@NYLS. It has been accepted for inclusion in Articles & Chapters by an authorized administrator of DigitalCommons@NYLS. "FRIEND TO THE MARTYR, A FRIEND TO THE WOMAN OF SHAME": THINKING ABOUT THE LAW, SHAME AND HUMILIATION* BY: PROFESSOR MICHAEL L. PERLIN•• & NAOMI M. WEINSTEIN, ESQ.*** ABSTRACT This Article considers the intersection between law, humiliation and shame, and how the law has the capacity to allow for, encourage, or (in some cases) remediate humiliation, or humiliating or shaming behavior. The need for new attention to be paid to this question has increased exponentially as society begins to also take international human rights mandates more seriously, especially-although certainly not exclusively-in the context of the recently-ratified United Nations Convention on the Rights of Persons with Disabilities, a convention that calls for "respect for inherent dignity," and characterizes "discrimination against any person on the basis of disability [as] a violation of the inherent dignity and worth of the human person ...." Humiliation and shaming contravene basic fundamental human rights and raise important constitutional questions implicating the due process and equal protection clauses. -
Microgram Journal, Vol 2, Number 1
Washington, D. C. Office of Science and Education Vol.II,No.1 Division of Laboratory Operations January 1969 INDEXISSUE CORRECTION 11 "Structure Elucidation of 'LBJ' , by Sander W. Bellman, John W. Turczan, James Heagy and Ted M. Hopes, Micro Gram .!., 3, 6-13 (Dec. 1968) Page 7, third and fourth sentences under Discussion: Change to read: "The melting point of the acid moiety found in step (g) was 148-150°c., compared to the litera ture, v~lue of 151°c for the melting point of benzilic acid (2); thus the benzilic acid melting point gives support to the proposed structure for 'LBJ'. Spectral evidence also supports the proposed structure". MICRO-GRAMREVISION Please re-number the pages of your copies of Micro-Gram, Volume I. Re-number pages bearing printing only. Vol ume I will then be numbered from page 1, the front page of issue No. 1, through page 189 the last page of issue No. 12. To help with this task, pages contained within each issue are as follows: Issue Number Page Through 1 1 8 2 9 29 3 30 32 4 33 66 5 67 79 6 80 97 7 98 120 8 121 128 9 129 136 10 137 157 11 158 170 12 171 189 CAUTION: Use of this publication should be restricted to forensic analysts or others having a legitimate need for this material. From the Archive Library of Erowid Center http://erowid.org/library/periodicals/microgram -2- CANNABIS ,·,-...__/' Attached is a copy of 11A Short Rapid Method for the Identification of Cannabis." The method was developed by Mro H.D. -
Tuning Drug Release from Polyoxazoline-Drug Conjugates T ⁎ J
European Polymer Journal 120 (2019) 109241 Contents lists available at ScienceDirect European Polymer Journal journal homepage: www.elsevier.com/locate/europolj Tuning drug release from polyoxazoline-drug conjugates T ⁎ J. Milton Harrisa, ,1, Michael D. Bentleya, Randall W. Moreaditha, Tacey X. Viegasa,1, Zhihao Fanga, Kunsang Yoona, Rebecca Weimera, Bekir Dizmanb, Lars Nordstiernac a Serina Therapeutics, Inc., 601 Genome Way, Suite 2001, Huntsville, AL 35806, USA2 b Sabanci University, Faculty of Engineering and Natural Sciences, Tuzla, 34956 İstanbul, Turkey2 c Department of Chemistry and Chemical Engineering, Chalmers University of Technology, SE-412 96 Göteborg, Sweden ARTICLE INFO ABSTRACT Keywords: Poly(2-oxazoline)-drug conjugates with drugs attached via releasable linkages are being developed for drug Poly(2-oxazoline) or POZ delivery. Such conjugates with pendent ester linkages that covalently bind drugs to the polymer backbone ex- Poly(2-ethyl-2-oxazoline) or PEOZ hibit significantly slower hydrolytic release rates in plasma than the corresponding PEG- and dextran-drug Pendent drugs conjugates. The slow drug release rates in-vitro of these POZ-drug conjugates contribute to extended in-vivo Degradable ester linkages pharmacokinetic profiles. In some instances, the release kinetics may be relatively sustained and ideal foronce-a- Pharmacokinetics week subcutaneous injection, whereas the native drug by itself may only have an in-vivo half-life of a few hours. Drug delivery Phenolic drugs The origin of this unusual kinetic and pharmacokinetic behavior is proposed here to involve folding of the POZ conjugate such that the relatively hydrophobic drug forms a central core, and the relatively hydrophilic polymer wraps around the core and slows enzymatic attack on the drug-polymer chemical linkage. -
Paralinguistic and Nonverbal Behaviour in Social Interactions: a Lens Model Perspective
Paralinguistic and Nonverbal Behaviour in Social Interactions: A Lens Model Perspective by Nicole Ann Ethier A thesis presented to the University of Waterloo in fulfillment of the thesis requirement for the degree of Doctor of Philosophy in Psychology Waterloo, Ontario, Canada, 2010 © Nicole Ann Ethier 2010 I hereby declare that I am the sole author of this thesis. This is a true copy of the thesis, including any required final revisions, as accepted by my examiners. I understand that my thesis may be made electronically available to the public. ii Abstract It is widely accepted in our society that people‟s paralinguistic (i.e., non-semantic characteristics of the voice) and nonverbal (i.e., posture, gestures, and facial expressions) behaviours play an important role in conveying information about their personality traits. Two particularly relevant traits include one‟s preferred levels of dominance and affiliation, which are the two major axes of the interpersonal circumplex. The current study investigates how dominance and affiliation are conveyed through paralinguistic and nonverbal behaviour using a lens model framework. Two major issues addressed by this framework include: 1) How do observers make inferences about people‟s dominance and affiliation using paralinguistic and nonverbal behaviours and 2) How do people‟s trait dominance and affiliation relate to these behavioural cues? To examine these two questions, we collected data from 114 opposite-sex dyads who worked together to complete a relatively unstructured collaborative task. The videotaped interactions were coded for specific paralinguistic (e.g., pitch, volume, resonance) and nonverbal (e.g., hand gestures, trunk posture, facial expressions) behaviours, in addition to coding more global displays of dominance and affiliation. -
Recent Developments in Cannabis Chemistry
Recent Developments in Cannabis Chemistry BY ALEXANDER T. SHULGIN, Ph.D. The marijuana plant Cannabis sativa contains a bewildering Introduction array of organic chemicals. As is true with other botanic species, there are representatives of almost all chemical classes present, including mono- and sesquiterpenes, carbohy- drates, aromatics, and a variety of nitrogenous compounds. Interest in the study of this plant has centered primarily on the resinous fraction, as it is this material that is invested with the pharmacological activity that is peculiar to the plant. This resin is secreted by the female plant as a protective agent during seed ripening, although it can be found as a microscopic exudate through the aerial portions of plants of either sex. The pure resin, hashish or charas, is the most potent fraction of the plant, and has served as the source material for most of the chemical studies. The family of chemicals that has been isolated from this source has been referred to as the cannabinoid group. It is unique amongst psychotropic materials from plants in that there are no alkaloids present. The fraction is totally nitro- gen-free. Rather, the set of compounds can be considered as analogs of the parent compound cannabinol (I), a fusion product of terpene and a substituted resorcinol. Beyond the scope of this present review are such questions as the distribution of these compounds within the plant, the bo- tanic variability resulting from geographic distribution, the diversity of pharmacological action assignable to the several Reprinted from Journal of Psychedelic Drugs, vol. II, no. 1, 197 1. 397 398 Marijuana: Medical Papers distinct compounds present, and the various preparations and customs of administration. -
Religious Use of Marijuana Cases
Summaries of Religious Use of Marijuana Cases Alabama regulate was conceivable, only that none had Rheuark v. State (1992) 601 So.2d 135 been proposed. Statutes prohibiting possession of marijuana and controlled substances did not violate freedom of California: religion rights of defendant claiming that he People v. Mullins (1975) 50 Cal. App 3d 61 worshipped plants as gods and used marijuana Facts: A deputy sheriff had gone to defendant's and psilocybin as religious practice. premises at night at the invitation of a man who Court compared Defendant’s argument to a past lived in a teepee on the property and who had told ruling on polygamy and quoting the cases the officer there were marijuana plants growing Reynolds v. United State 98 U.S. 145 and there. The officer took two plants as samples to Cleveland v. United States 329 U.S. 14 which in be tested and, three days later, went back to the sum says that morality is defined by statute and property with a search warrant. Defendant's Congress and not the individual’s perceptions of property is not enclosed or surrounded by a morality. Therefore because the possession of fence. From Ten Mile Creek Road there is a marijuana is unlawful, it doesn’t matter that the driveway that leads onto defendant's property. Defendant used it for religious use. There is no gate at the entrance to the property. At the entrance there is a sign reading “Universal Arizona: Life Church of Christ Light.” Defendant's wife People v. Hardesty (2009) 214 P.3d 1004 testified that on and prior to May 30, 1972, there FACTS: Hardesty was driving his van at night was a “no trespassing” sign at the entrance to the when an officer stopped him because one property; that a church known as “Universal Life headlight was out. -
Preparative Isolation of Cannabinoids from Cannabis Sativa by Centrifugal Partition Chromatography
See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/244594577 Preparative Isolation of Cannabinoids from Cannabis sativa by Centrifugal Partition Chromatography Article in Journal of Liquid Chromatography & Related Technologies · December 2004 DOI: 10.1081/JLC-200028170 CITATIONS READS 47 2,645 5 authors, including: Arno Hazekamp Robert Verpoorte Bedrocan BV Leiden University 50 PUBLICATIONS 975 CITATIONS 732 PUBLICATIONS 21,203 CITATIONS SEE PROFILE SEE PROFILE Some of the authors of this publication are also working on these related projects: The effects of inhaled delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD) on pain relief and subjective effects in fibromyalgia patients View project All content following this page was uploaded by Robert Verpoorte on 20 June 2014. The user has requested enhancement of the downloaded file. Cannabis; extracting the medicine i Arno Hazekamp Cannabis; extracting the medicine Proefschrift Universiteit Leiden ISBN 978-90-9021997-4 Printed by PrintPartners Ipskamp B.V., Amsterdam, The Netherlands Paper cover: Cannabis Pur 100% (250 grams) from Grafisch Papier, The Nederlands. Photo cover: Dutch medicinal cannabis, variety “Bedrocan”. ii Cannabis; extracting the medicine Proefschrift Ter verkrijging van de graad van Doctor aan de Universiteit Leiden, op gezag van de Rector Magnificus prof. mr. P. F. van der Heijden, hoogleraar in de faculteit der Rechtsgeleerdheid, volgens besluit van het College voor Promoties te verdedigen op woensdag 5 september 2007 klokke 15.00 uur door Arno Hazekamp Geboren op 15 maart 1976 te Bilthoven iii Promotiecommissie Promotor Prof. dr. R. Verpoorte Referent Dr. C. Giroud (Institut Universitaire de Médecine Légale, Lausanne, Switzerland) Overige leden Prof.