Catalog # Aliquot Size

U231-911-05 3 x 5 nmol U231-911-20 3 x 20 nmol U231-911-50 3 x 50 nmol

UBE2N (UBC13) siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # U231-911

Lot # Z2109-29

Specificity Formulation UBE2N (UBC13) siRNAs are designed to specifically knock- The siRNAs are supplied as a lyophilized powder and down human UBE2N (UBC13) expression. shipped at room temperature.

Product Description Reconstitution Protocol UBE2N (UBC13) siRNA is a pool of three individual Briefly centrifuge the tubes (maximum RCF 4,000g) to synthetic siRNA duplexes designed to knock-down collect lyophilized siRNA at the bottom of the tube. human UBE2N (UBC13) mRNA expression. Each siRNA is Resuspend the siRNA in 50 µl of DEPC-treated water 19-25 bases in length. The accession number is (supplied by researcher), which results in a 1x stock NM_003348. solution (10 µM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot Gene Aliases 1x stock solutions for storage. UBC13; UbcH-ben Related Products Storage and Stability The lyophilized powder is stable for at least 4 weeks at Product Name Catalog Number room temperature. It is recommended that the UBE2N (UBC13) U231-30H lyophilized and resuspended siRNAs are stored at or UBE2O Protein U232-30G below -20oC. After resuspension, siRNA stock solutions ≥2 UBE2R1 Protein U234-30H µM can undergo up to 50 freeze-thaw cycles without UBE2R2 (UBC3B) Protein U235-30H significant degradation. For long-term storage, it is UBE2U Protein U237-30G recommended that the siRNA is stored at -70oC. For most UBE2V2 Protein U239-30H favorable performance, avoid repeated handling and UBE2W (UBC16) Protein U240-30G multiple freeze/thaw cycles. UBE2Z (USE1) Protein U241-31H Anti-UBE2N (UBC13) U231-63R Scientific Background UBE2N (UBC13) or -conjugating enzyme E2N is a member of the E2 ubiquitin-conjugating enzyme family UBE2N (UBC13) siRNA Set I siRNA duplexes targeted against three exon regions which plays a role in DNA post-replication repair. UBE2N gene encodes a novel human E2 that may be involved in Catalog # U231-911 protein degradation in the muscles and testis (1). UBE2N Lot # Z2109-29 plays a role in initiation of homologous recombination Packaging Specifications 2.5 nmol/tube for 3 x 5nmol during double-strand break repair (2). UBE2N activity may have therapeutic uses in controlling inflammatory Format Lyophilized powder Stability 1yr at -70oC from date of shipment responses and it is also important in the induction of Storage & Shipping The lyophilized powder is stable for at immune responses. least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at References or below -20oC. After resuspension, 1. Yamaguchi, T. et.al: Cloning and expression of cDNA siRNA stock solutions ≥2 µ M can encoding a human ubiquitin-conjugating enzyme undergo up to 50 freeze-thaw cycles without significant degradation. For similar to the Drosophila bendless gene product. J. long-term storage, it is recommended Biochem. 120: 494-497, 1996. that the siRNA is stored at -70oC. For 2. Zhao, G. Y. et.al: A critical role for the ubiquitin- most favorable performance, avoid repeated handling and multiple conjugating enzyme Ubc13 in initiating homologous freeze/thaw cycles. recombination. Molec. Cell 25: 663-675, 2007.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.