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Posters A-Z Adams, Sophie Investigating the role of centrosome amplification in exosome-mediated 47 remodelling of the pancreatic tumour microenvironment. Adhami-Mojarad, Bahareh CEP19 Cooperates with FOP and CEP350 to drive early stages of 48 ciliogenesis program Akhshi, Tara Hedgehog pathway can regulate ciliogenesis 49 Alieva, Irina Ultrastructural features of the endotheliocyte centrosome and its 50 possible involvement into the functional activity of endothelium Alvarez Rodrigo, Ines Dissecting Polo kinase recruitment to the centrosome 51 Amargant, Farners Characterization of the human sperm centrosome and its role in fertility 52 Andersen, Jens S Identification of candidate phosphorylation sites regulating centriole 53 biogenesis Arbi, Marina Geminin family members take center stage in the gene network 54 controlling multiple cilia formation Arnandis, Teresa Non-cell autonomous invasion mediated by centrosome amplification 55 Au, Kin Chung Franco GAS2L1 Is a Centriole-associated Protein That Functions in 56 Centrosome Disjunction Aydogan, Mustafa Kinetics of daughter centriole growth in flies: How does distal-end 57 contribute to setting the centriole size? Page 15 EMBO Conference: Centrosomes and Spindle Pole Bodies B, Binshad SCF-FBXW7 E3 ligase regulates centriole duplication by targeting 58 HsSAS-6 Banterle, Niccolò Probing SAS-6 assembly dynamics with high speed Atomic Force 59 Microscopy Basavaraju, Shivakumar CHK1- dependent mechanisms of centrosome amplification after DNA 60 damage Beyer, Tina Cluap1: Isoform-specific protein complex analysis and 61 CRISPR/Cas9-mediated knock-out revealed new functions of Cluap1 in cellular dynamics. Bianchi, Sarah Presenter: Vakonakis, John The C. elegans centriolar protein SAS-5 binds microtubules to facilitate 62 organelle assembly Bierkamp, Christiane The role of ninein during cellular differentiation and mouse skin 63 morphogenesis Bornens, Michel Presenter: Allier, Cedric The role of centrosome in cell division cycle as monitored by lens-free 64 microscopy Bose, Arunabha Regulation of centrosome duplication by 14-3-3? 65 Breslow, David A comprehensive portrait of cilia and ciliopathies from a CRISPR-based 66 screen for Hedgehog signaling Brouhard, Gary C. elegans microtubules are highly dynamic and have non-canonical 67 lattices Page 16 Posters A-Z Bui, Hien Age selective segregation of peroxisomes during stem cell division is 68 mediated by the mitotic spindle Burkhalter, Martin D. Imbalanced mitochondrial function induces heterotaxy via aberrant 69 ciliogenesis Camargo Ortega, German Akna, a new component of the interphase centrosome, regulates 70 neurogenesis by microtubule anchoring Cernohorska, Marketa A cell-free assay for microtubule doublet assembly 71 Chan, Vincent Presenter: Chao-Chu, Jennifer To study the effect of NEK2 on chromosome instability in hepatocellular 72 carcinoma Chinen, Takumi Divergent acentrosomal pathways ensure spindle bipolarity in human 73 cancer cells Cosenza, Marco R. Asymmetric centriole rosettes in bipolar spindles cause chromosome 74 missegregation in cancer Curinha, Ana Functional Analysis of Rbm26 75 Cury, Nathália Transcriptionally, karyotypic and cell cycle consequences of microtubule 76 disassembly in acute lymphoblastic leukemia Damen, Mareike Presenter: Bazzi, Hisham Centrioles are required for the development of the skin epidermis and 77 hair follicles Dammermann, Alexander Centrioles initiate cilia assembly but are dispensable for maturation and 78 maintenance in C. elegans Page 17 EMBO Conference: Centrosomes and Spindle Pole Bodies Delgehyr, Nathalie An apical actin network induced by the beating of motile ependymal 79 cilia protects basal bodies against the shear stress exerted by the cilia Dobbelaere, Jeroen Identification and characterization of cilia proteins in Drosophila 80 melanogaster Duenas-Sanchez, Rafael Role of Bem2 in the integrity of the Spindle Position Checkpoint 81 Dwivedi, Devashish Hook2 is a dynein-dynactin adaptor required for cytokinesis 82 Erpf, Anna Christina A novel protein required for bipolar spindle formation in the one-cell 83 stage Caenorhabditis elegans embryo Farrell, KC Moderating influence of centrosome on microtubule polymer and cell 84 mechanics Firat-Karalar, Elif Nur The retinal degeneration gene product CCDC66 functions in cilium 85 formation and is regulated by centriolar satellites Fitzpatrick, Alexa Characterization of Retinitis Pigmentosa GTPase Regulator Mutations in 86 Patients with Syndromic Phenotypes: Linking Primary and Motile Ciliopathies Florindo, Claudia Mob1: defining cell polarity for proper cell division 87 Ford, Jodie From spirals to rings: protein engineering of C. elegans SAS-6 88 Frikstad, Kari-Anne M. A CEP104-CSPP1 complex is required for formation of primary cilia 89 competent in Hedgehog-signaling Page 18 Posters A-Z Gambarotto, Davide Presenter: Gambarotto, Davide; Cernohorska, Marketa; Hamel, Virginie; Guichard, Paul Nanometric protein cartography of centrioles using Expansion 90 microscopy Gartenmann, Lisa Revealing Centriole Secrets – Examining The Protein Interactions That 91 Form The Centriole In Vivo Giessl, Andreas Pericentrin, identified at the basal-body complex in mammalian 92 photoreceptor cells, interacts with Nesprin protein Syne-2 in the retina Gogendeau, Delphine Down-regulation of different transition zone proteins in Paramecium 93 induces antagonistic effects on cilia stability Gomes, Beatriz Multivalent interactions of the scaffold protein SPD-5 underlie PCM 94 assembly in C. elegans Gönczy, Pierre Presenter: Pierron, Marie Uncovering mechanisms of centriole elimination during C. elegans 95 oogenesis Gopalakrishnan, Jay Presenter: Mariappan, Aruljothi ¬¬Premature activation of extra centrosomes by a CPAP-tubulin 96 inhibitor selectively prevents cancer cellular proliferation Goranci-Buzhala, Gladiola Cilia induction to prevent proliferation of glioma stem cells in vitro 97 Goundiam, Oumou Analysis of centrosome alterations in Epithelial Ovarian Cancers (EOCs) 98 reveals new types of defects Gunzelmann, Judith Cooperation of the receptor Spc72, the microtubule polymerase Stu2 99 and g-TuSC at microtubule minus ends Page 19 EMBO Conference: Centrosomes and Spindle Pole Bodies Gupta, Hindol Human SAS-6 regulates centriole duplication by interacting with 100 gamma-tubulin ring complex proteins Häfner, Julia Function of Cdk5rap2 during enucleation of mammalian erythroblasts 101 Haren, Laurence Role of GCP4, 5 and 6 in gamma-TuRC assembly 102 Hatzopoulos, Georgios Development of monobodies for dissecting centriole assembly 103 Hoffmann, Ingrid Presenter: Meyer, Annalena Ccdc61 is a novel centriolar satellite component that regulates spindle 104 assembly and symmetry Hoffmann, Ingrid The Cullin4-DDB1 E3 Ubiquitin Ligase Complex regulates 105 Plk4- dependent centriole duplication Howard, Ryan Investigating the relationship between poly (ADP-ribose) polymerases 106 and centrosome clustering in cancer cells Hsu, Wen-hsin Adducin-1 is essential for spindle pole integrity through its interaction 107 with TPX2 Ito, Daisuke The yeast centriole-less centrosome reveals an ancestral role for the 108 pericentriolar matrix in centriole biogenesis Jao, Li-En Regulation of centrosome maturation by localized RNA 109 Joachimiak, Ewa Phosphorylation sites on gamma-tubulin are essential for the structure 110 and functions of basal bodies in Tetrahymena Page 20 Posters A-Z Julner, Alexander High-throughput approach to understanding spindle pole body 111 dynamics Kamranvar, Siamak A. Lack of integrin is associated with centrosome abnormality and 112 subsequent genomic instability Kapoor, Varun Automated microtubule tracking: A potpourri of biology, physics, 113 computer vision and machine learning Kessler, Kristin The centrosomal protein NIMA-related serine/threonine kinase -Nek 114 1- at cilia of sensory cells Khouj, Ebtissal Centrin2 calcium-binding capacity is required for cilium formation but 115 not for NER activity. Kim, Jaeyoun Presenter: Rhee, Kunsoo Pericentrin regulation of centriole association and separation during M 116 phase Kitagawa, Daiju Crucial functions of the cartwheel structure in human procentriole 117 formation Koca Caydasi, Ayse Spindle Pole Bodies as signaling hubs for the Spindle Position 118 Checkpoint of budding yeast Koo, Stephanie Chuay-Yeng A novel TAO kinase inhibitor delays mitosis and induces mitotic 119 catastrophe in breast cancer cells with supernumerary centrosomes Kovarikova, Petra Presenter: Kovarikova, Petra; Bernatik, Ondrej Tau tubulin kinase 2 phosphorylations in cilia initiation 120 Page 21 EMBO Conference: Centrosomes and Spindle Pole Bodies Kumar, Dhivya The dynamic hub of the cell: centriolar satellites control the organization 121 of the centrosome Kurtulmus, Bahtiyar LRRC45 organises centriolar satellites for ciliogenesis 122 Laláková, Jana Exploring the role of centrosome inheritance in cell fate determination in 123 human stem-like cells Le Borgne, Pierrick Functional study of novel human PCD candidate genes in Paramecium 124 Le Guennec, Maeva Native architecture of Paramecium tetraurelia centriole 125 Le Roux-Bourdieu, Morgan How does centrosome age impact the mitotic spindle? 126 Leong, Su Ling In vitro reconstitution of microtubule nucleation by the gamma-tubulin 127 small complex and the Mto1/2 complex reveals a novel function for Mzt1 Lerit, Dorothy Regulation of transposable element activity and microtubule 128 organization in Drosophila Li, Qiuhong Beatrice Presenter: Li, Qiuhong Beatrice; Kantsadi, Anastassia
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  • PP2A-B′ Holoenzyme Substrate Recognition, Regulation and Role in Cytokinesis

    PP2A-B′ Holoenzyme Substrate Recognition, Regulation and Role in Cytokinesis

    OPEN Citation: Cell Discovery (2017) 3, 17027; doi:10.1038/celldisc.2017.27 ARTICLE www.nature.com/celldisc PP2A-B′ holoenzyme substrate recognition, regulation and role in cytokinesis Cheng-Guo Wu1,2,8, Hui Chen1,8, Feng Guo1,8,9, Vikash K Yadav3,8, Sean J Mcilwain4, Michael Rowse1, Alka Choudhary5, Ziqing Lin6, Yitong Li1, Tingjia Gu1, Aiping Zheng1,10, Qingge Xu6, Woojong Lee1, Eduard Resch7, Benjamin Johnson1, Jenny Day1, Ying Ge6, Irene M Ong4, Mark E Burkard5, Ylva Ivarsson3,*, Yongna Xing1,2,* 1McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin at Madison, School of Medicine and Public Health, Madison, WI, USA; 2Biophysics Program, University of Wisconsin at Madison, Madison, WI, USA; 3Department of Chemistry—BMC, Uppsala University, Uppsala, Sweden; 4Biostatistics and Medical Informatics, Wisconsin Institutes of Medical Research, University of Wisconsin at Madison, School of Medicine and Public Health, Madison, WI, USA; 5Department of Medicine, Hematology/Oncology, UW Carbone Cancer Center, University of Wisconsin at Madison, School of Medicine and Public Health, Madison, WI, USA; 6Department of Cell and Regenerative Biology, Human Proteomic Program, School of Medicine and Public Health, Madison, WI, USA; 7Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Project Group Translational Medicine and Pharmacology TMP, Frankfurt am Main, Germany Protein phosphatase 2A (PP2A) is a major Ser/Thr phosphatase; it forms diverse heterotrimeric holoenzymes that counteract kinase actions. Using a peptidome that tiles the disordered regions of the human proteome, we identified proteins containing [LMFI]xx[ILV]xEx motifs that serve as interaction sites for B′-family PP2A regulatory subunits and holoenzymes.
  • Is Cep70, a Centrosomal Protein with New Roles in Breast Cancer Dissemination and Metastasis, a Facilitator of Epithelial-Mesenchymal Transition?

    Is Cep70, a Centrosomal Protein with New Roles in Breast Cancer Dissemination and Metastasis, a Facilitator of Epithelial-Mesenchymal Transition?

    Is Cep70, a centrosomal protein with new roles in breast cancer dissemination and metastasis, a facilitator of epithelial-mesenchymal transition? Pedro A. Lazo 1,2 1 Experimental Therapeutics and Translational Oncology Program, Instituto de Biología Molecular y Celular del Cáncer, Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Salamanca, Salamanca, Spain 2 Instituto de Investigación Biomédica de Salamanca (IBSAL), Hospital Universitario de Salamanca, Salamanca, Spain Running title: Cep70 and EMT Disclosures: None declared. Contact address: [email protected] 1 Introduction Microtubules are driving mechanisms of chromosomes, intracellular organelle movement, and cell shape and motility. Microtubules are organized on centrosomes, which are assembled in microtubule-organizing centers (MTOC). In mitosis there is no nuclear envelope and centromeres associated to microtubules are mainly involved in chromosome redistribution into daughter cells. In differentiated cells the microtubule- organizing centers are dispersed in the cytoplasm (non centrosomal (ncMTOC)) and interact with the minus end of microtubules through γ-tubulin. 1 However, it is not known if the microtubule contribution to tumor biology is only by facilitating tumor aneuploidy. During tumor dissemination, a process not linked to cell division, important changes take place in cell shape and motility. Microtubules are very dynamic because of their inherent structural instability, and this plasticity facilitates their reorganization during the epithelial-mesenchymal