Pisolithus Aureosericeus Fungal Planet Description Sheets 423

422 Persoonia – Volume 36, 2016

Pisolithus aureosericeus Fungal Planet description sheets 423

Fungal Planet 452 – 4 July 2016 Pisolithus aureosericeus M.P. Martín, Kaewgrajang, Phosri & Watling, sp. nov.

Etymology. From Latin aureus and sericeus, referring to the colour and (2013) clearly grouped the new sequences with species of texture of the peridium. P. aurantioscabrosus from Malaysia collected under Shorea Classiﬁcation — Sclerodermataceae, Boletales, Agaricomy macropera. However, the specimens of P. aureosericeus form cetes. a cluster together as a group of their own, and were collected under Hopea odorata. Moreover, the peridium surface is slightly Macroscopic characteristics — Basidiomes subglobose to velvety and golden yellow, and the basidiospores are strongly broadly ellipsoid, gasterocarp, 10–50 mm, sessile. Peridium ornamented with wedge-shaped extensions giving a very rough surface slightly velvety, golden yellow at ﬁrst, later buff to snuff appearance; although Pisolithus spores are ornamented this brown. Rhizomorphs at the base, small, 0.8–1.3 mm high present feature is rather uncommon in the genus and helps to × 0.3–0.5 diam. Gleba orange-brown become ferruginous delimit this new taxon under the microscope. powdery mass at maturity by the breakdown of the peridioles. KUFKUFF002F002 (1),(1), KU351835,KU351835, PP Peridioles subglobose to broadly ellipsoid, 0.2–0.8 × 1.0–1.2 mm diam, thin-walled, surface smooth, bright yellow or greenish CladeClade 15,15, Pisolithus KUFF002KUFF002 (2),(2), KU351836,KU351836, PP sp.sp. nonov.v. aureosericeusaureosericeus KUFF001,KUFF001, KKU351837,U351837, H yellow, later a snuff-brown powdery mass when they mature. 100 Microscopic characteristics — Constituent hyphae inter- KUFKUFF003F003 (1),(1), KU351838,KU351838, PP twined, cream to ochraceous, thin-walled, 2–3.5 mm broad, septate without encrustation, clamp-connections present. Ba 5151 KUKUFF003FF003 ( (2),2), KU KU351839,351839, P sidia not seen. Spores globose to subglobose, 8.5–10 × 8–11 KUKUFF003FF003 (3),(3), KU351840,KU351840, PP 100 µm excluding ornamentation, pale brown, densely ornamented 2 Clade 11, P. aurantioscabrosus with pyramidal spines (0.5–0.8 µm long). Clade 13, P. indicus Typus. Thailand, Nakhon Ratchasima, alt. 470 m, N14°29'59" E101°56'22", 100 2 Clade 1, Pisolithus sp. on clay loam soil, under Hopea odorata trees, 8 Aug. 2012, T. Kaewgrajang 69 100 KUFF001 (holotype Herbarium Kasetsart University, ITS sequence GenBank 5 Clade 12, P. abditus KU351837, MycoBank MB851695). (Clade 10, AF374624, Pisolithus sp.) hailand Additional materials examined. T , Nakhon Ratchasima, alt. 470 m, 95 7 Clade 9, P. microcarpus N14°29'59" E101°56'22", on clay loam soil, under Hopea odorata trees, 8 Aug. 51 89 2012, T. Kaewgrajang KUFF002 (Herbarium Kasetsart University, ITS se- 65 2 Clade 8 (Pisolithus sp.) quences GenBank KU351835, KU351836); ibid., T. Kaewgrajang KUFF003 AF004732 (under Pisolithus sp.) (Herbarium Kasetsart University, ITS sequences GenBank KU351838, KU 98 <50 351839, KU351840). 22 Clade 7, P. albus complex

Notes — The genus Pisolithus has for a long time been (Clade 10, AF374646, Pisolithus sp.) considered a genus of species with mainly a xerophytic life- (Clade 9, AF440867, P. microcarpus) strategy, being found in shrub-land, woodland-clearings, even 98 6 Clade 2 wasteland, and all generally on highly mineral soils (Pilát 1958). , P. marmoratus 99 For many years the dark coloured, elongated, narrow stemmed 7 Clade 3, P. calongei 73 Pisolithus kisslingii was perhaps the only species linked to 9 Clade 6 P. tinctorius 91 , tropical areas being described from the rain forest in Sumatra 53 12 Clade 5 P. orientalis (Fischer 1906); although, specimens of this genus had been , 54 located amongst the dried collections of the late John H. Corner 12 Clade 4, P. arhizus 74 now housed in the herbarium of the Royal Botanic Garden, 6 Clade 14, P. capsulifer Edinburgh. However, after intensive work in the last few years in more tropical plant-communities has demonstrated a wealth FM213344S, Scleroderma citrinum of species of Pisolithus present certainly in South-East Asia. GU373495, Suillus luteus The present study delimits a further species, similar in colour 2.0 to P. aurantioscabrosus, but differing markedly in the smoother One of the 100 equally most parsimony trees obtained after and slightly velvety outer surface of the exoperidium in contrast a heuristic search of the ITS sequence alignment (PAUP to the erect squamules of the former. Phylogenetic analyses v. 4.0b10). Following Phosri et al. (2012) and Martín et al. (parsimony), based on three collections (six specimens/six (2013), sequences of Suillus luteus and Scleroderma citrinum sequences) of P. aurosericeus, and previously published data, were included as outgroup. Pisolithus sequences were distri mainly from Martin et al. (2002), Phosri et al. (2012), Martín et al. buted in 15 main clades, clade number after Martin et al. (2002); percentage of bootstrap values (> 50 %) are indicated on the branches. The P. aureosericeus clade is marked with a grey Colour illustrations. Thailand, Nakhon Ratchasima (T. Kaewgrajang); a. basidiome (KUFF001); b. basidiomes (KUFF001) detail to shown the square (H: Holotype; P: Paratypes); the accession number peridioles; c, d. spores (KUFF001). Scale bars = 10 mm (basidiomes), 1 μm from EMBL/GenBank or UNITE databases are indicated to (spores). the rest of terminals.

María P. Martín, Departamento de Micología, Real Jardín Botánico-CSIC, Plaza de Murillo 2, 28014 Madrid, Spain; e-mail: [email protected] Tharnrat Kaewgrajang, Department of Forest Biology, Faculty of Forestry, Kasetsart University, 50 Ngamwongwan Rd, Latyao, Chatuchak, Bangkok 10900, Thailand; e-mail: [email protected] Cherdchai Phosri, Faculty of Science, Nakhon Phanom University, 214, Moo 12, Nittayo Road, Nong Yart Sub-district, Muang District, Nakhon Phanom, 48000, Thailand; e-mail: [email protected] Roy Watling, Caledonian Mycological Enterprises, Vrelah, 26 Blinkbonny Avenue, Edinburgh, EH4 3HU, Scotland, UK; e-mail: [email protected]