Improving Quality and Efficiency in Dental Hygiene Journal of Genrotological Nursing, Vol
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Pathological and Therapeutic Approach to Endotoxin-Secreting Bacteria Involved in Periodontal Disease
toxins Review Pathological and Therapeutic Approach to Endotoxin-Secreting Bacteria Involved in Periodontal Disease Rosalia Marcano 1, M. Ángeles Rojo 2 , Damián Cordoba-Diaz 3 and Manuel Garrosa 1,* 1 Department of Cell Biology, Histology and Pharmacology, Faculty of Medicine and INCYL, University of Valladolid, 47005 Valladolid, Spain; [email protected] 2 Area of Experimental Sciences, Miguel de Cervantes European University, 47012 Valladolid, Spain; [email protected] 3 Area of Pharmaceutics and Food Technology, Faculty of Pharmacy, and IUFI, Complutense University of Madrid, 28040 Madrid, Spain; [email protected] * Correspondence: [email protected] Abstract: It is widely recognized that periodontal disease is an inflammatory entity of infectious origin, in which the immune activation of the host leads to the destruction of the supporting tissues of the tooth. Periodontal pathogenic bacteria like Porphyromonas gingivalis, that belongs to the complex net of oral microflora, exhibits a toxicogenic potential by releasing endotoxins, which are the lipopolysaccharide component (LPS) available in the outer cell wall of Gram-negative bacteria. Endotoxins are released into the tissues causing damage after the cell is lysed. There are three well-defined regions in the LPS: one of them, the lipid A, has a lipidic nature, and the other two, the Core and the O-antigen, have a glycosidic nature, all of them with independent and synergistic functions. Lipid A is the “bioactive center” of LPS, responsible for its toxicity, and shows great variability along bacteria. In general, endotoxins have specific receptors at the cells, causing a wide immunoinflammatory response by inducing the release of pro-inflammatory cytokines and the production of matrix metalloproteinases. -
Effect of Sonic and Mechanical Toothbrushes on Subgingival Microbial Flora: a Comparative in Vivo Scanning Electron Microscopy Study of 8 Subjects
Isa ni Research Effect of sonic and mechanical toothbrushes on subgingival microbial flora: A comparative in vivo scanning electron microscopy study of 8 subjects Karen B, Williams, MS, RDHVCharles M, Cobb, DDS, PhDVHeidi J. Taylor, MS, Alan R. Brown, DDSVKimberly Krust Bray, MS, Objedives; The purpose of this initial study was to evaluafe fhe effects of bcfii a sonic and a mechanical focfhbrush versus fhe effects of no freatment on depth of subgingival penetrafion of epifhelial and footh- assooiafed bacferia. Method and materials: Eight adult subjects exhibiting advanced chronic pericdontitis with at leasf 3 single-rocfed feeth thaf were in separate sextanfs wiffi facial pockefs a 4 mm and s 8 mm and fhaf required exfraction consfituted the expérimentai sample. Teeth were either subjected to 15 sec- onds of brushing wifh a mechanical toothbrush or a sonic tocfhbrush or leff untreated. The tesf toofh and fhe associafed soft fissue wall of the periodonfai pocket were removed as a single unit. Samples were processed and coded for blind examinafion by scanning elecfron microsoopy. Distribufionai and morpho- logic characferisfics of dominanf bacteria wifh specific emphasis on spirochetes were evaluafed for boffi epithelial- and foofh-associafed plaque. Results: No differences were found in morphotypes or disfribu- fional and aggregafional characteristics of epifhelial-associafed microbes in the f- to 3-mm subgingival zone befween fhe mechanical and sonic tocf h bru s h-freated groups and the control group. Both toothbrush groups featured disrupfion of microbes fhat extended up to 1 mm subgingivally. Roof surfaces on the sonic-treated samples appeared plaque-free at low magnificafion; however, af 4,70Dx, afhin layer of mixed morphofypes and intact spirochefes was found supragingivally and slighfly subgingivally. -
Toothbrush Could Cure Bad Pet Breath “But Cats Are Another Story,” Animals Also Need She Added
3DLIFE Page Editor: Jim Barr, 754-0424 LAKE CITY REPORTER LIFE SUNDAY, JANUARY 20, 2013 3D PETS Toothbrush could cure bad pet breath “But cats are another story,” Animals also need she added. brushing at home, Harvey said that’s because cats’ mouths are smaller, their regular checkups. teeth sharper and they could care less about bonding with a human By SUE MANNING during designated tooth time. Associated Press Keith said she took it slow when she began brushing the LOS ANGELES — Dogs and teeth of her 8-year-old greyhound cats can’t brush, spit, gargle or Val. She started with one tooth at floss on their own. So owners a time and used a foamless fla- who want to avoid bad pet breath vored gel that dogs can swallow. will need to lend a hand. “She started to nibble (on “Brushing is the gold stan- the toothbrush) and I rubbed dard for good oral hygiene at it on her front teeth. I didn’t home. It is very effective, but make a big deal out of it. I didn’t some dogs and more cats don’t worry about brushing the first appreciate having something half dozen times. It was just a in their mouth,” said Dr. Colin little bonding thing. Eventually, Harvey, a professor of surgery I brushed one tooth. Now she and dentistry in the Department stands there and lets me brush of Clinical Studies for the all her teeth,” she said. University of Pennsylvania’s The gel doesn’t require water School of Veterinary Medicine. -
Dentinal Hypersensitivity: a Review
Dentinal Hypersensitivity: A Review Abstract Dentinal hypersensitivity is generally reported by the patient after experiencing a sharp pain caused by one of several different stimuli. The pain response varies substantially from one person to another. The condition generally involves the facial surfaces of teeth near the cervical aspect and is very common in premolars and canines. The most widely accepted theory of how the pain occurs is Brannstrom’s hydrodynamic theory, fluid movement within the dentinal tubules. The dental professional, using a variety of diagnostic techniques, will discern the condition from other conditions that may cause sensitive teeth. Treatment of the condition can be invasive or non-invasive in nature. The most inexpensive and efficacious first line of treatment for most patients is a dentifrice containing a desensitizing active ingredient such as potassium nitrate and/or stannous fluoride. This review will address the prevalence, diagnosis, and treatment of dentinal hypersensitivity. In addition the home care recommendations will focus on desensitizing dentifrices. Keywords: Dentinal hypersensitivity, hydrodynamic theory, stannous fluoride, potassium nitrate Citation: Walters PA. Dentinal Hypersensitivity: A Review. J Contemp Dent Pract 2005 May;(6)2:107-117. © Seer Publishing 1 The Journal of Contemporary Dental Practice, Volume 6, No. 2, May 15, 2005 Introduction The prevalence of dentinal hypersensitivity Dentifrices and mouth rinses are routinely used has been reported over the years in a variety as a delivery system for therapeutic agents of ways: as greater than 40 million people such as antimicrobials and anti-sensitivity in the U.S. annually1, 14.3% of all dental agents. Therapeutic oral care products are patients2, between 8% and 57% of adult dentate available to assist the patient in the control of population3, and up to 30% of adults at some time dental caries, calculus formation, and dentinal during their lifetime.4 hypersensitivity to name a few. -
Desensitizing Agent Reduces Dentin Hypersensitivity During Ultrasonic Scaling: a Pilot Study Dentistry Section
Original Article DOI: 10.7860/JCDR/2015/13775.6495 Desensitizing Agent Reduces Dentin Hypersensitivity During Ultrasonic Scaling: A Pilot Study Dentistry Section TOMONARI SUDA1, HIROAKI KOBAYASHI2, TOSHIHARU AKIYAMA3, TAKUYA TAKANO4, MISA GOKYU5, TAKEAKI SUDO6, THATAWEE KHEMWONG7, YUICHI IZUMI8 ABSTRACT of the dentin hypersensitivity agent. Evaluation of effects on Background: Dentin hypersensitivity can interfere with optimal dentin hypersensitivity was determined by a questionnaire and periodontal care by dentists and patients. The pain associated visual analog scale (VAS) pain scores after ultrasonic scaling. with dentin hypersensitivity during ultrasonic scaling is intolerable The statistical analysis was performed using the paired Student for patient and interferes with the procedure, particularly during t-test and Spearman rank correlation coefficient. supportive periodontal therapy (SPT) for patients with gingival Results: The desensitizing agent reduced the mean VAS pain recession. score from 69.33 ± 16.02 at baseline to 26.08 ± 27.99 after Aim: This study proposed to evaluate the desensitizing effect of application. The questionnaire revealed that >80% patients the oxalic acid agent on pain caused by dentin hypersensitivity were satisfied and requested the application of the desensitizing during ultrasonic scaling. agent for future ultrasonic scaling sessions. Materials and Methods: This study involved 12 patients who Conclusion: This study shows that the application of the oxalic were incorporated in SPT program and complained of dentin acid agent considerably reduces pain associated with dentin hypersensitivity during ultrasonic scaling. We examined the hypersensitivity experienced during ultrasonic scaling. This availability of the oxalic acid agent to compare the degree of pain control treatment may improve patient participation and pain during ultrasonic scaling with or without the application treatment efficiency. -
The Inpatient Rehabilitation Facility – Patient Assessment Instrument (Irf-Pai) Training Manual
THE INPATIENT REHABILITATION FACILITY – PATIENT ASSESSMENT INSTRUMENT (IRF-PAI) TRAINING MANUAL: EFFECTIVE 10/01/2012 For patient assessments performed when a patient is discharged on or after October 1, 2012, the IRF-PAI Training Manual: Effective 10/01/2012 is the version of the manual that must be used when performing the patient assessment and recording that assessment data on the IRF-PAI. Copyright 2001- 2003 UB Foundation Activities, Inc. (UBFA, Inc.) for compilation rights; no copyrights claimed in U.S. Government works included in Section I, portions of Section IV, Appendices G and I, and portions of Appendices B, C, E, F, and H. All other copyrights are reserved to their respective owners. Copyright ©1993-2001 UB Foundation Activities, Inc. for the FIM Data Set, Measurement Scale, Impairment Codes, and refinements thereto for the IRF-PAI, and for the Guide for the Uniform Data Set for Medical Rehabilitation, as incorporated or referenced herein. The FIM mark is owned by UBFA, Inc. IRF-PAI Training Manual Effective 10/01/2012 CMS Help Desk: For all questions related to recording data on the IRF patient assessment instrument (IRF- PAI) or using the Inpatient Rehabilitation Validation Entry (IRVEN) Software: Phone: 1-800-339-9313 Fax: 1-888-477-7871 Email: [email protected] Coverage Hours: 8:00am (ET) to 8:00pm (ET) Monday through Friday Please note: When sending an email, to receive priority, please include 'IRF Clinical' in the subject line. Toll-Free Customer Service for IRF billing/payment questions: See link below to the Medicare Learning Network. A document on that page provides toll-free customer service numbers for the fiscal intermediaries and Medicare Administrative Contractors (MACs). -
Peri-Implantitis: a Review of the Disease
DENTISTRY ISSN 2377-1623 http://dx.doi.org/10.17140/DOJ-2-117 Open Journal Review Peri-Implantitis: A Review of the Disease *Corresponding author and Report of a Case Treated with Zeeshan Sheikh, Dip.Dh, BDS, MSc, PhD Department of Dentistry Allograft to Achieve Bone Regeneration University of Toronto Room 222 Fitzgerald Building 150 College Street Toronto, ON M5S 3E2, Canada Haroon Rashid1#, Zeeshan Sheikh2#*, Fahim Vohra3, Ayesha Hanif1 and Michael Glogauer2 Tel. +1-416-890-2289 E-mail: [email protected]; [email protected] #These authors contributed equally Volume 2 : Issue 3 1Division of Prosthodontics, College of Dentistry, Ziauddin University, Karachi, Pakistan Article Ref. #: 1000DOJ2117 2Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Canada 3College of Dentistry, Division of Prosthodontic, King Saud University, Riyadh, Saudi Arabia Article History Received: September 20th, 2015 Accepted: October 1st, 2015 ABSTRACT Published: October 5th, 2015 Dental implants offer excellent tooth replacement options however; peri-implantitis can limit their clinical success by causing failure. Peri-implantitis is an inflammatory process Citation around dental implants resulting in bone loss in association with bleeding and suppuration. Rashid H, Sheikh Z, Vohra F, Hanif A, Glogauer M. Peri-implantitis: a review Dental plaque is at the center of its etiology, and in addition, systemic diseases, smoking, and of the disease and report of a case parafunctional habits are also implicated. The pathogenic species associated with peri-implan- treated with allograft to achieve bone titis include, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Tanner- regeneration. Dent Open J. 2015; ella forsythia. The goal in the management of peri-implantitis is the complete resolution of 2(3): 87-97. -
Intergeneric Communication in Dental Plaque Biofilms
JOURNAL OF BACTERIOLOGY, Dec. 2000, p. 7067–7069 Vol. 182, No. 24 0021-9193/00/$04.00ϩ0 Copyright © 2000, American Society for Microbiology. All Rights Reserved. NOTES Intergeneric Communication in Dental Plaque Biofilms HUA XIE,1* GUY S. COOK,2 J. WILLIAM COSTERTON,3 GREG BRUCE,4 4 5 TIMOTHY M. ROSE, AND RICHARD J. LAMONT School of Dentistry, Meharry Medical College, Nashville, Tennessee1; Bacterin Inc.,2 and Center for Biofilm Engineering, Montana State University,3 Bozeman, Montana; and Department of Pathobiology4 and Department of Oral Biology,5 University of Washington, Seattle, Washington Received 27 June 2000/Accepted 21 September 2000 Downloaded from Dental plaque is a complex biofilm that accretes in a series of discrete steps proceeding from a gram-positive streptococcus-rich biofilm to a structure rich in gram-negative anaerobes. This study investigated information flow between two unrelated plaque bacteria, Streptococcus cristatus and Porphyromonas gingivalis. A surface pro- tein of S. cristatus caused repression of the P. gingivalis fimbrial gene (fimA), as determined by a chromosomal fimA promoter-lacZ reporter construct and by reverse transcription-PCR. Signaling activity was associated jb.asm.org with a 59-kDa surface protein of S. cristatus and showed specificity for the fimA gene. Furthermore, P. gingivalis was unable to form biofilm microcolonies with S. cristatus. Thus, S. cristatus is capable of modulating virulence gene expression in P. gingivalis, consequently influencing the development of pathogenic plaque. at MONTANA STATE UNIV AT BOZEMAN on October 13, 2009 The study of the ability of bacterial cells to communicate which were grown in Trypticase Peptone broth supplemented with one another and coordinate behavior is a burgeoning field with yeast extract (5 mg/ml) and 0.5% glucose at 37°C aerobi- with relevance to a number of microbial ecosystems (5, 6, 11, cally; Treponema denticola GM-1, which was cultured for 5 12, 17). -
Toothbrush, Its Design and Modifications : an Overview
Journal of Current Medical Research and Opinion Received 12-07-2020 | Accepted 11-08-2020 | Published Online 12-08-2020 DOI: https://doi.org/10.15520/jcmro.v3i08.322 ISSN (O) 2589-8779 | (P) 2589-8760 CMRO 03 (08), 570−578 (2020) REVIEW ARTICLE Toothbrush, its Design and Modifications : An Overview ∗ Silky Mehta1 C.V.Sruthi Vyaasini2 Lucky Jindal3 Vishnu Sharma4 Talika Jasuja5 1MDS, Paedodontics and Abstract Preventive Dentistry, Faridabad, Tooth brush has been an integral part of a daily routine across many Haryana cultures around the world from the times of antiquity to the 21st century. 2 PG Student, Department of Over the years, several types of toothbrush has been invented. Some Periodontics and Implantology, of the them are useful for physically and mentally handicapped J.N.Kapoor DAV (C) Dental College, Yamuna Nagar, Haryana children The aim of this review article is to describe toothbrush design and various modifications that have been made in the several 3Senior Lecturer, Department of Paedodontics and Preventive years. Dentistry, JCD Dental College, Keywords: Bristle,Cleaning, Head,Toothbrush Sirsa Haryana 4Private Consultant, Yamuna Nagar, Haryana 5Intern, J.N.Kapoor DAV (C) Dental College, Yamuna Nagar, Haryana 1 INTRODUCTION design of brush, method of brushing, time taken and also on supervision in care of small children. (3) ffective plaque control facilitates good gingi- Over its long history, the toothbrush has evolved to val and periodontal health, prevents tooth de- become a scientifically designed tool using modern Ecay and preserves oral health for lifetime. (1) ergonomic designs and safe and hygienic materials The various methods commonly used for plaque that benefit us all. -
Sensitive Teeth Causes & Treatment Options
SENSITIVE TEETH CAUSES & TREATMENT OPTIONS TEETHMATE™ DESENSITIZER The future is now… create hydroxyapatite HAVING SENSITIVE TEETH SENSITIVITY CAN HAVE VARIOUS CAUSES, AND THERE ARE DIFFERENT TREATMENT OPTIONS IS A POPULATION-WIDE The conditions for dentin sensitivity are that the dentin There are many treatment strategies and even more must be exposed and the tubules must be open on both products that are used to eliminate dentin sensitivity. the oral and the pulpal sides. Patients suffering from However, today there is unfortunately still no universally dentin sensitivity describe the pain sensation as a severe, accepted treatment method. The many variables, the PROBLEM sharp, usually short-term pain in the tooth. placebo effect, and the many treatment methods get Holland et al.1 characterise dentin sensitivity as a short, in the way of the design of studies4. In most cases, the sharp pain resulting from exposed dentin in response to treatment of dentin sensitivity starts with the application various stimuli. These stimuli are typically thermal, i.e. by of desensitizing toothpaste. After this or simultaneously, evaporation, tactile, i.e. by osmosis or chemically, or not the treatment can be supplemented with one or more And something every practice has to deal with due to any other form of dental pathological defect. treatment options5. Patients with dentin sensitivity may react to air blown But what exactly do we mean by sensitive teeth? How many from the air-syringe or to scratching with a probe on the PREVALENCE patients report to dental practices with this problem and is this tooth surface. Of course, it is essential to rule out possible According to several publications6 7 8 9 10, dentin sensitivity figure in line with the prevalence? What are the different causes causes of the pain other than dentin sensitivity. -
Dental Plaque: a Host- Associated Biofilm
DENTAL PLAQUE DENTAL PLAQUE: A HOST- ASSOCIATED BIOFILM • Dental plaque is a host-associated biofilm. • The significance of the biofilm environment has been increasingly recognized in recent years because the environment itself may alter properties of the microorganisms. • The biofilm community is initially formed through bacterial interactions with the tooth and then through physical and physiologic interactions among different species within the microbial mass . Macroscopic Structure and Composition of Dental Plaque • Dental plaque can be defined as the soft deposits that form the biofilm adhering to the tooth surface or other hard surfaces in the oral cavity, including removable and fixed restorations. • Materia alba refers to soft accumulations of bacteria and tissue cells that lack the organized structure of dental plaque and are easily displaced with a water spray. • Calculus is a hard deposit that forms by mineralization of dental plaque and is generally covered by a layer of unmineralized plaque. • Dental plaque is broadly classified as Supragingival plaque is found at or above the gingival margin; the supragingival plaque in direct contact with the gingival margin is referred to as marginal plaque. • Subgingival plaque is found below the gingival margin, between the tooth and the gingival sulcular tissue. • The different regions of plaque are significant to different processes associated with diseases of the teeth and periodontium. • For example, marginal plaque gingivitis. Supragingival plaque and tooth-associated subgingival plaque are critical in calculus formation and root caries . Subgingival plaque soft tissue destruction different forms of periodontitis. Dental plaque is composed primarily of microorganisms . Cultivation studies, in which bacteria are isolated and characterized in the laboratory, indicate that more than 500 distinct microbial species are found in dental plaque Nonbacterial microorganisms that are found in plaque include Mycoplasma species, yeasts, protozoa, and viruses . -
Plaque Removal Effect by Round-Shaped Toothbrush
Research Article Journal of Dentistry and Oral Biology Published: 04 Jan, 2021 Plaque Removal Effect by Round-Shaped Toothbrush Kosuke Muraoka1*, Masaki Morishita1, Ryota Kibune1, Makoto Yokota2 and Shuji Awano1 1Department of Oral Function, Division of Clinical Education Development and Research, Kyushu Dental University, Kitakyushu, Japan 2Yokota Dental Academy, Fukuoka, Japan Abstract Background and Objective: Plaque control is the most important factor in periodontal therapy. Especially patient with periodontitis needs good plaque control. We have developed round-shaped toothbrush and have tested its effectiveness of plaque removal. Materials and Methods: We have selected ten volunteer students from Kyushu dental university dental hygienist course, and all volunteers were informed and consented. All members had healthy and periodontal tissue. Before examination, plaque index was calculated by O’Leary’s Plaque Control Record (PCR). After 10 min brushing by bath method, plaque index were calculated again. Results: Round-shaped toothbrush revealed significantly high plaque removal rate on lingual side of mandibular molars. Conclusion: Round-shaped toothbrush might have possibility to lead to the perfect plaque control. Keywords: Round-shaped toothbrush; Oral hygiene; Tooth brushing; Dental plaque Introduction It is widely accepted that most significant cause of periodontal disease is bacterial plaque [1,2]. The importance of plaque control in periodontal therapy had been recognized widely. Rosling et al. [3] were reported the importance of brushing method to cleanse supragingival plaque to in order to OPEN ACCESS stabilize the periodontal tissue. In addition, Axelsson and lindhe [4] were emphasized the important *Correspondence: role of continuous plaque control even in the maintenance phase. To maintain good prognosis by Kosuke Muraoka, Department of Oral periodontal therapy and long-term periodontal tissue health, daily plaque control by the patients Function, Division of Clinical Education themselves is essential.