DOCSLIB.ORG

Arginase Inhibition by Piceatannol-3'-O-Β-D-Glucopyranoside Improves Endothelial Dysfunction Via Activation of Endothelial Nitr

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Arginase Inhibition by Piceatannol-3'-O-Β-D-Glucopyranoside Improves Endothelial Dysfunction Via Activation of Endothelial Nitr INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 31: 803-810, 2013 Arginase inhibition by piceatannol-3'-O-β-D-glucopyranoside improves endothelial dysfunction via activation of endothelial nitric oxide synthase in ApoE-null mice fed a high-cholesterol diet AINIENG WOO1*, WOOSUNG SHIN1*, TO DAO CUONG3, BYUNGSUN MIN3, JEONG HYUNG LEE2, BYUNG HWA JEON4 and SUNGWOO RYOO1 Departments of 1Biology, 2Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon 200-701; 3College of Pharmacy, Catholic University, Daegu 712-702; 4Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-131, Republic of Korea Received October 31, 2012; Accepted December 28, 2012 DOI: 10.3892/ijmm.2013.1261 Abstract. Elevated plasma cholesterol is a hallmark of numerous incubation neither altered the contractile response to a high K+ cardiovascular diseases that are closely linked to endothelial solution nor the relaxation response to SNP. When analyzing dysfunction indicating decreased nitric oxide (NO) production the L-arginine content using high-performance liquid in the endothelium. It has been previously demonstrated that chromatography, PG incubation increased the intracellular piceatannol-3'-O-β-D-glucopyranoside (PG) inhibits arginase L-arginine concentration. PG administration in the drinking activity and reciprocally regulates NO production. Here, we water significantly reduced fatty streak formation in ApoE-/- aimed to ascertain whether PG ameliorates vascular function mice fed an HCD. These data indicate that PG improves the in wild-type (WT) and atherogenic model mice [apolipo- pathophysiology of cholesterol-mediated endothelial dysfunc- protein E-null mice (ApoE-/-)] and to investigate the possible tion. Therefore, we conclude that the development of PG as a underlying mechanism. Preincubation of aortic vessels from novel effective therapy for preventing atherosclerotic diseases WT mice fed a normal diet (ND) with PG attenuated vaso- is warranted. constriction response to U46619 and phenylephrine (PE), while the vasorelaxant response to acetylcholine (Ach) was Introduction markedly enhanced in an endothelium-dependent manner. However, the endothelium-independent NO donor, sodium Elevated plasma cholesterol, such as native low-density nitroprusside (SNP), did not change vessel reactivity. In lipoprotein (LDL) and oxidized LDL (oxLDL), is a hallmark thoracic aorta from ApoE-/- mice, a high-cholesterol diet of numerous cardiovascular diseases including hypercholes- (HCD) induced an increase in arginase activity, a decrease in terolemia, atherosclerosis, hypertension, heart failure, and NO release and an increase in reactive oxygen species genera- diabetes. These diseases are closely linked with endothelial tion that was reversed by treatment with PG. The effect of PG dysfunction indicating decreased nitric oxide (NO) production was associated with enhanced stability of the eNOS dimer and in the endothelium. In the vasculature, NO is a vasoprotective was not dependent on the expression levels of arginase II and molecule and plays a central role in vascular homeostasis by eNOS proteins, although eNOS expression was increased in regulating vasoreactivity, platelet activation, leukocyte adhe- ApoE-/- mice fed an HCD. Furthermore, PG treatment attenu- sion and smooth muscle cell migration and proliferation (1). ated the PE-dependent contractile response, and significantly It is well established that endothelial arginase constrains improved the Ach-dependent vasorelaxation response in aortic the activity of endothelial nitric oxide synthase (eNOS) by rings from ApoE-/- mice fed an HCD. On the other hand, PG substrate depletion, thereby reducing NO bioavailability and contributing to vascular diseases. oxLDL, the primary patho- genic lipid in atherogenesis, activates human endothelial cell arginase II by stimulating the dissociation of arginase II from Correspondence to: Professor Sungwoo Ryoo, Department of microtubules and also by inducing arginase II mRNA tran- Biology, Kangwon National University, Kangwondae-gil 1, Chuncheon, scription (2). Furthermore, atherogenic-prone apolipoprotein Kangwon-do 200-701, Republic of Korea E-null (ApoE-/-) mice treated with an arginase inhibitor exhibit E-mail: [email protected] restored NO bioavailability and endothelial function, reactive *Contributed equally oxygen species (ROS) production, and an arterial compliance similar to that observed in wild-type (WT) mice (3). Therefore, Key words: arginase, piceatannol-3'-O-β-D-glucopyranoside, endo- endothelial arginase may be a novel target for therapeutic drug thelial dysfunction, endothelial nitric oxide synthase, apolipoprotein design for vascular diseases such as atherosclerosis (3). E-null mice Rhubarb is the rhizome of Rheum undulatum and is commonly distributed in Asia. Many components of the 804 WOO et al: PICEATANNOL-3'-O-β-D-GLUCOPYRANOSIDE IMPROVES ENDOTHELIAL DYSFUNCTION rhizome possess diverse biological activities and have been to agonist across vessel rings. The dose response to the vaso- reported as being able to counter allergic (4) and diabetic states constrictors, PE (10-9-10 -4 M) and U46619 (10-9-10 -5 M), was (5), as having anti-oxidant properties (4), and as functioning as performed first. This was followed by the dose response to a vasorelaxant (6). Piceatannol, one of the active components the vasodilators, acetylcholine (Ach, 10-9-10 -5 M) and SNP of rhubarb, was recently found to inhibit lipooxygenase activity (10 -9-10 -5 M) after pre-constriction with PE (10-6 M). At the (7) and VSMC proliferation and migration (8). Recently, we end of the experiments, the NO-dependency of vasorelaxation reported that piceatannol-3'-O-β-D-glucopyranoside (PG) is a was confirmed by adding the inhibitor of guanylate cyclase potent inhibitor of arginase isoforms. PG inhibited the arginase [1H-[1,2,4]oxadizolo[4,3-a]quinoxalin-1-one (ODQ), 10-6 M)]. isoforms in a dose-dependent manner, resulting in augmented NO production by enhancing eNOS dimer stabilization (9). Arginase activity. Tissue lysates were prepared using lysis Based on these data, we hypothesized that PG regulates buffer (50 mM Tris-HCl, pH 7.5, 0.1 mM EDTA and protease vascular function. Therefore, we examined whether PG inhibitors) by homogenization at 4˚C followed by centrifuga- improves NO/ROS production and endothelial dysfunction in tion for 20 min at 14,000 x g at 4˚C. The supernatants were ApoE-/- mice fed a high-cholesterol diet (HCD). We also inves- used to assay for arginase activity as previously described (10). tigated whether arginase inhibition by PG restores L-arginine bioavailability and attenuates fatty streak formation in this Estimation of NO or ROS generation using DAF-FM or DHE atherogenic mouse model. in isolated mice aorta. Mice aortic rings were isolated and incubated overnight at 37˚C in 5% CO2 in Dulbecco's modified Materials and methods Eagle's medium containing 2% FBS and antibiotics (1X) in the presence of PG (50 µmol/l) (10). The fluorescence from the Animals. Twenty 10-week-old male wild-type (WT) aortic endothelium was measured at different time intervals (C57BL/6J) and ApoE-/- mice (Dae Han Biolink Co.) were under microscopy (9). studied. The study was approved in accordance with the Guide for the Care and Use of Laboratory Animals (Institutional Determination of intracellular L-arginine concentrations. Review Board, Kangwon National University). The intracellular concentration of L-arginine was determined by high-performance liquid chromatography (HPLC) using Protocol. To determine the effect of PG on vascular reactivity, pre-column derivatization with o-phthalaldehyde (OPA) by we studied aortic rings isolated from 20 male C57BL/6J WT modification of a previously published method (11). L-arginine mice fed a normal diet (ND) and 20 male ApoE-/- mice fed (100 µmol/l) was added to the cell lysate (0.5 ml) as an internal an HCD (D12108C; Research Diet Inc., USA) for 6 weeks. standard. The samples were extracted on solid-phase extraction Aortic rings were incubated with or without PG (50 µmol/l) cartridges (CBA Bond Elute; Varian, Inc.). Recovery rates were for 18 h as previously described (9). For the pathological assay, 87.5±3.9%. Eluates were dried over nitrogen and resuspended PG was administered in the drinking water to ApoE-/- mice in double-distilled water for HPLC analysis. HPLC was for 6 weeks when the mice were started on the HCD. Given performed on a computer-controlled Waters chromatography that each mouse consumed ~10 ml water/day this represented system (M600E) consisting of an automatic injector (M7725i; a daily dose of ~500 µg/mouse/day of PG. Waters Co.) and a fluorescence detector (FP-1520; Jasco Co.) located in the Central Laboratory of Kangwon National Western blot analysis. Aortic vessel lysates were subjected University. Samples were incubated for exactly 1 min with OPA to SDS-PAGE, and densitometry of the bands was conducted reagent (5.4 mg/ml OPA in borate buffer, pH 8.4, containing using NIH ImageJ (9). To analyze the ratio of eNOS dimer 0.4% 2-mercaptoethanol) before automatic injection for the to monomer, proteins were separated using low-temperature HPLC. The OPA derivative of L-arginine was separated on a SDS-PAGE followed by western blot analysis (9). 150x4.6 mm, 3.5-µm Zorbax Eclipse XDB-C18 column with the fluorescence detector set at Ex 340 nm and Em 450 nm. Aortic vascular tension assay. Male C57BL/6J mice fed an Samples were eluted from the column with 0.96% citric acid/
Load more

Recommended publications
  • Synthase from Rat Liver
    Proc. Nati. Acad. Sci. USA Vol. 89, pp. 5361-5365, June 1992 Biochemistry Purification of a distinctive form of endotoxin-induced nitric oxide synthase from rat liver (endothelium-derived relaxing factor/L-arginine/calmodulin) TOM EVANS, ADAM CARPENTER, AND JONATHAN COHEN* Department of Infectious Diseases, Royal Postgraduate Medical School, Du Cane Road, London W12 ONN, United Kingdom Communicated by Robert F. Furchgott, March 6, 1992 (received for review November 18, 1991) ABSTRACT An endotoxin-induced form of nitric oxide We are interested in the hepatic form of the NO synthase, synthase (EC 1.14.23) was purified to homogeneity from rat which is induced by the administration of endotoxin (8). liver by sequential anion-exchange chromatography and afflin- Livers from untreated animals show minimal NO synthase ity chromatography using 2',5'-ADP-Sepharose. The enzyme activity. NO production in the liver seems to suppress protein has a subunit molecular mass of 135 kDa as determined by synthesis and protects the liver against damage (18). We have SDS/PAGE, a maximum specific activity of 462 nmol of purified this enzyme to homogeneity and show that it is citrulline formed from arginine per min per mg, and a K. for strongly stimulated by calmodulin, whereas its activity is arginine of 11 jIM. The enzyme was strongly stimulated by the only affected to a small degree by free calcium concentra- addition of calmodulin with an EC50 of 2 nM, but removal of tions-even in the presence ofcalmodulin. Thus, this hepatic free calcium from the assay medium only reduced activity by endotoxin-induced NO synthase is distinct from that de- 15%.
    [Show full text]
  • Effect of Arginase Inhibition on Ischemia-Reperfusion Injury in Patients with Coronary Artery Disease with and Without Diabetes Mellitus
    Effect of Arginase Inhibition on Ischemia-Reperfusion Injury in Patients with Coronary Artery Disease with and without Diabetes Mellitus Oskar Ko¨ vamees*, Alexey Shemyakin, John Pernow Department of Medicine, Karolinska Institutet, Stockholm, Sweden Abstract Background: Arginase competes with nitric oxide synthase for their common substrate L-arginine. Up-regulation of arginase in coronary artery disease (CAD) and diabetes mellitus may reduce nitric oxide bioavailability contributing to endothelial dysfunction and ischemia-reperfusion injury. Arginase inhibition reduces infarct size in animal models. Therefore the aim of the current study was to investigate if arginase inhibition protects from endothelial dysfunction induced by ischemia-reperfusion in patients with CAD with or without type 2 diabetes (Clinical trial registration number: NCT02009527). Methods: Male patients with CAD (n = 12) or CAD + type 2 diabetes (n = 12), were included in this cross-over study with blinded evaluation. Endothelium-dependent vasodilatation was assessed by flow-mediated dilatation (FMD) of the radial artery before and after 20 min ischemia-reperfusion during intra-arterial infusion of the arginase inhibitor (Nv-hydroxy-nor- L-arginine, 0.1 mg/min) or saline. Results: The forearm ischemia-reperfusion was well tolerated. Endothelium-independent vasodilatation was assessed by sublingual nitroglycerin. Ischemia-reperfusion decreased FMD in patients with CAD from 12.765.2% to 7.964.0% during saline administration (P,0.05). Nv-hydroxy-nor-L-arginine administration prevented the decrease in FMD in the CAD group (10.364.3% at baseline vs. 11.563.6% at reperfusion). Ischemia-reperfusion did not significantly reduce FMD in patients with CAD + type 2 diabetes. However, FMD at reperfusion was higher following nor-NOHA than following saline administration in both groups (P,0.01).
    [Show full text]
  • Effects of Γ-Aminobutyric Acid on the Erectogenic Properties of Sildenafil
    Available online on www.ijtpr.com International Journal of Toxicological and Pharmacological Research 2017; 9(3); 234-243 ISSN: 0975-5160 Research Article Effects of γ-Aminobutyric Acid on the Erectogenic Properties of Sildenafil Adefegha S A1*, Oyeleye S I1,2 Oboh G1 1Functional food and Nutraceutical Laboratory, Department of Biochemistry, Federal University of Technology, Akure, P.M.B. 704, Akure 340001, Nigeria 2Department of Biomedical Technology, Federal University of Technology, Akure, P.M.B. 704, Akure 340001, Nigeria Available Online:25th July, 2017 ABSTRACT Erectile dysfunction (ED) is a disorder of increasing socio-economic burden. Therapeutic drugs such as sildenafil have been in use for the treatment of ED, but with their associated side effects. γ-aminobutyric acid (GABA) is a neurotransmitter with possible vasodilatory properties. In this study, the effect of GABA on the erectogenic properties of sildenafil was investigated. Aqueous solution of GABA and sildenafil (1 mM) was separately prepared as well as the mixtures of both (75% GABA + 25% sildenafil; 50% GABA + 50% sildenafil; 25% GABA + 75% sildenafil). Thereafter, the in vitro effects of all the studied samples on the activities of arginase, angiotensin-I converting enzyme (ACE) and acetylcholinesterase (AChE) were investigated. The results revealed that all the samples inhibited arginase, ACE and AChE activities. Considering the various combinations, 25% GABA + 75% sildenafil had the highest arginase inhibitory effect, 50% GABA + 50% sildenafil showed the highest ACE inhibiting effect, while 25% GABA + 75% sildenafil exhibited the highest AChE inhibitory effect. Therefore, the observed enzyme inhibiting effect of sildenafil, GABA and their various combinations on rat penile arginase, ACE and AChE activities could be part of the mechanism by which they elicit their erectogenic properties.
    [Show full text]
  • Competitive Metabolism of L-Arginine: Arginase As a Therapeutic Target In
    JBR Journal of Biomedical Research，2011，25(5):299-308 http://elsevier.com/wps/ Review find/journaldescription.cws_ home/723905/description#description Competitive metabolism of L-arginine: arginase as a therapeutic ☆ target in asthma * Jennifer M. Bratt, Amir A. Zeki, Jerold A. Last, Nicholas J. Kenyon Department of Internal Medicine, Division of Pulmonary and Critical Care and Sleep Medicine, University of California, Davis, CA 95616, USA Received 25 April 2011, Revised 24 June 2011, Accepted 21 July 2011 Abstract Exhaled breath nitric oxide (NO) is an accepted asthma biomarker. Lung concentrations of NO and its amino acid precursor, L-arginine, are regulated by the relative expressions of the NO synthase (NOS) and arginase iso- forms. Increased expression of arginase I and NOS2 occurs in murine models of allergic asthma and in biopsies of asthmatic airways. Although clinical trials involving the inhibition of NO-producing enzymes have shown mixed results, small molecule arginase inhibitors have shown potential as a therapeutic intervention in animal and cell culture models. Their transition to clinical trials is hampered by concerns regarding their safety and potential tox- icity. In this review, we discuss the paradigm of arginase and NOS competition for their substrate L-arginine in the asthmatic airway. We address the functional role of L-arginine in inflammation and the potential role of arginase inhibitors as therapeutics. Keywords: nitric oxide, L-arginine, arginase, nor-NOHA, nitrosation, nitric oxide synthase INTRODUCTION from accumulation of collagens in the submucosal and [3] Asthma is a common disease characterized by a reticular basement membrane . The airway remod- syndrome of persistent airway inflammation and re- eling and resultant reduction in overall lung function versible airway obstruction.
    [Show full text]
  • Increased Arginine Amino Aciduria/Urea Cycle Disorder
    Newborn Screening ACT Sheet Increased Arginine Amino Aciduria/Urea Cycle Disorder Differential Diagnosis: Argininemia (ARG) Condition Description: The urea cycle is the enzyme cycle whereby ammonia is converted to urea. In argininemia, defects in arginase, a urea cycle enzyme, may result in hyperammonemia. Take the Following IMMEDIATE Actions • Contact family to inform them of the newborn screening result and ascertain clinical status (poor feeding, vomiting, lethargy, tachypnea). • Immediate telephone consultation with pediatric metabolic specialist. (See attached list.) • Evaluate the newborn (poor feeding, vomiting, lethargy, hypotonia, tachypnea, seizures and signs of liver disease). • If any sign is present or infant is ill, IMMEDIATELY initiate emergency treatment for hyperammonemia in consultation with metabolic specialist. • Transport to hospital for further treatment in consultation with metabolic specialist. • Initiate timely confirmatory/diagnostic testing and management, as recommended by specialist. • Initial testing: immediate plasma ammonia, plasma quantitative amino acids, and urine orotic acid. • Repeat newborn screen if second screen has not been done. • Provide family with basic information about hyperammonemia. • Report findings to newborn screening program. Diagnostic Evaluation: Specific diagnosis is made by plasma quantitative amino acid analysis revealing increased arginine and urine orotic acid analysis revealing increased orotic acid, respectively. Blood ammonia determination may also reveal hyperammonemia. Clinical
    [Show full text]
  • Arginase Deficiency
    Arginase Deficiency Lauren Ballard, Alex Belardo, Lainie Boyle, Abby Dryden, Rachel Kirchner, M’Lynn Gwinner, Shreya Nalluri, & Julia Slogar Service Learning Initiative in Biochemistry 5614 (Autumn 2018) Argininemia: An autosomal recessive disorder Video on Inheritance Patterns HNEkidshealth. “Autosomal Recessive Inheritance - Genetics.” YouTube, YouTube, 30 Mar. 2015, www.youtube.com/watch?v=Nv6qUsKYodA. Argininemia ● Inherited disorder that causes arginine (amino acid) and ammonia to build up in the blood ● The deficiency usually becomes evident by 3 years of age ● Occurs once in every 300,000 to 1,000,000 individuals ● Caused by a mutation in the ARG1 gene, which encodes the enzyme arginase Baby's first foods: Where to begin? Digital image retrieved ● Inherited in an autosomal recessive pattern October 31, 2018 from https://www.kabritausa.com/blog/babys-first-foods-begin/ Protein Metabolism Amino acids Peptide Protein How Your Body Uses Amino Acids as Proteins. Digital image retrieved October 31, 2018 https://socratic.org/questions/amino-acids-as-monomers-of-protein ● Amino Acids are the building blocks of proteins ● Dietary protein must be broken down into amino acids Protein Metabolism + NH4 Urea (waste) Dietary Protein Amino Acids Carbon Glucose backbone (energy) Intracellular Protein Argininemia and the Urea Cycle ● Disorder of the urea cycle ● Our bodies produce ammonia as metabolic waste - Ammonia is highly toxic and must be discarded ● The urea cycle in the liver converts ammonia to urea, a less toxic compound Urea Cycle. Digital image retrieved October 31, 2018 from https://www.slideshare.net/YESANNA/urea-cycle-44200147 What is Argininemia? ● Disorder of the urea cycle ● Dysfunctional arginase enzyme disrupts final step of urea cycle ● Build-up of ammonia in the blood X Urea Cycle.
    [Show full text]
  • Increased Arginase II Activity Contributes to Endothelial Dysfunction Through Endothelial Nitric Oxide Synthase Uncoupling in Aged Mice
    EXPERIMENTAL and MOLECULAR MEDICINE, Vol. 44, No. 10, 594-602, October 2012 Increased arginase II activity contributes to endothelial dysfunction through endothelial nitric oxide synthase uncoupling in aged mice Woosung Shin1, Dan E. Berkowitz2,3 ArgII. These results might be associated with and Sungwoo Ryoo1,4 increased L-arginine bioavailability. Collectively, these results suggest that ArgII may be a valuable 1Department of Biology target in age-dependent vascular diseases. College of Natural Sciences Kangwon National University Keywords: aging; arginase II; endothelial nitric oxide Chuncheon 200-701, Korea synthase uncoupling; small interfering RNA; vascular 2Department of Anesthesiology and Critical Care Medicine diseases 3Department of Biomedical Engineering Johns Hopkins Medical Institutions Baltimore, MD 21287, USA Introduction 4Corresponding author: Tel, 82-33-250-8534; Fax, 82-33-251-3990; E-mail, [email protected] Cardiovascular disease is the leading cause of http://dx.doi.org/10.3858/emm.2012.44.10.068 morbidity and mortality in both industrialized and developing countries. Despite effective treatments Accepted 27 July 2012 for several established cardiovascular risk factors, Available Online 2 August 2012 such as hypertension and hypercholesterolemia, the incidence of cardiovascular disease is predicted Abbreviations: ABH, 2 (S)-amino-6-boronohexanoic acid; Ach, to increases as the population ages. Characteristic acetylcholine; Arg I, arginase I; ArgII, arginase II; DAF, 4-amino- events in the aging cardiovascular system include 5-methylamino-2', 7'-difluorescein; DHE, dihydroethidium; L-arg, vascular stiffness (Meaume et al., 2001), enhanced G L-arginine; L-NAME, L-N -nitroarginine methyl ester; MnTBAP, Mn reactive oxygen species (ROS) production (Finkel (III)tetrakis (4-benzoic acid) porphyrin chloride; ns, not significant; and Holbrook, 2000; Brandes et al., 2005), and ROS, reactive oxygen species; siArgII, siRNA to arginase II decreased nitric oxide (NO) bioavailability (Adler et al., 2003; Csiszar et al., 2008).
    [Show full text]
  • Analysis of Nitrogen Utilization Capability During the Proliferation and Maturation Phases of Norway Spruce (Picea Abies (L.) H.Karst.) Somatic Embryogenesis
    Article Analysis of Nitrogen Utilization Capability during the Proliferation and Maturation Phases of Norway Spruce (Picea abies (L.) H.Karst.) Somatic Embryogenesis Julia Dahrendorf 1, David Clapham 2 and Ulrika Egertsdotter 1,3,* 1 Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-901 83 Umeå, Sweden; [email protected] 2 BioCentre, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden; [email protected] 3 G.W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA * Correspondence: [email protected]; Tel.: +1-404-663-6950 Received: 1 April 2018; Accepted: 22 May 2018; Published: 24 May 2018 Abstract: Somatic embryogenesis (SE) is a laboratory-based method that allows for cost-effective production of large numbers of clonal copies of plants, of particular interest for conifers where other clonal propagation methods are mostly unavailable. In this study, the effect of L-glutamine as an organic nitrogen source was evaluated for three contrasted media (containing NH4 + NO3 without glutamine, or glutamine + NO3, or glutamine without inorganic nitrogen) during proliferation and maturation of Norway spruce somatic embryos through analyses of activities of the key enzymes of nitrogen metabolism: nitrate reductase (NR), glutamine synthetase (GS) and arginase. A major change in nitrogen metabolism was indicated by the increased activity of GS from zero in the proliferation stage through maturation to high activity in somatic embryo-derived plantlets; furthermore, NR activity increased from zero at the proliferation stage to high activity in maturing embryos and somatic-embryo derived plantlets.
    [Show full text]
  • Arginase Isoforms Expression of Nitric Oxide Synthase and Populations Of
    Microenvironments in Tuberculous Granulomas Are Delineated by Distinct Populations of Macrophage Subsets and Expression of Nitric Oxide Synthase and This information is current as Arginase Isoforms of September 26, 2021. Joshua T. Mattila, Olabisi O. Ojo, Diane Kepka-Lenhart, Simeone Marino, Jin Hee Kim, Seok Yong Eum, Laura E. Via, Clifton E. Barry III, Edwin Klein, Denise E. Kirschner, Sidney M. Morris, Jr., Philana Ling Lin and JoAnne L. Downloaded from Flynn J Immunol 2013; 191:773-784; Prepublished online 7 June 2013; doi: 10.4049/jimmunol.1300113 http://www.jimmunol.org/content/191/2/773 http://www.jimmunol.org/ Supplementary http://www.jimmunol.org/content/suppl/2013/06/07/jimmunol.130011 Material 3.DC1 References This article cites 66 articles, 18 of which you can access for free at: http://www.jimmunol.org/content/191/2/773.full#ref-list-1 by guest on September 26, 2021 Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606.
    [Show full text]
  • Arginase As a Potential Biomarker of Disease Progression: a Molecular Imaging Perspective
    International Journal of Molecular Sciences Review Arginase as a Potential Biomarker of Disease Progression: A Molecular Imaging Perspective Gonçalo S. Clemente 1 , Aren van Waarde 1, Inês F. Antunes 1, Alexander Dömling 2 and Philip H. Elsinga 1,* 1 Department of Nuclear Medicine and Molecular Imaging, University Medical Center Groningen, University of Groningen, 9713 GZ Groningen, The Netherlands; [email protected] (G.S.C.); [email protected] (A.v.W.); [email protected] (I.F.A.) 2 Department of Drug Design, Groningen Research Institute of Pharmacy, University of Groningen, 9713 AV Groningen, The Netherlands; [email protected] * Correspondence: [email protected]; Tel.: +31-50-361-3247 Received: 2 July 2020; Accepted: 23 July 2020; Published: 25 July 2020 Abstract: Arginase is a widely known enzyme of the urea cycle that catalyzes the hydrolysis of L-arginine to L-ornithine and urea. The action of arginase goes beyond the boundaries of hepatic ureogenic function, being widespread through most tissues. Two arginase isoforms coexist, the type I (Arg1) predominantly expressed in the liver and the type II (Arg2) expressed throughout extrahepatic tissues. By producing L-ornithine while competing with nitric oxide synthase (NOS) for the same substrate (L-arginine), arginase can influence the endogenous levels of polyamines, proline, and NO•. Several pathophysiological processes may deregulate arginase/NOS balance, disturbing the homeostasis and functionality of the organism. Upregulated arginase expression is associated with several pathological processes that can range from cardiovascular, immune-mediated, and tumorigenic conditions to neurodegenerative disorders. Thus, arginase is a potential biomarker of disease progression and severity and has recently been the subject of research studies regarding the therapeutic efficacy of arginase inhibitors.
    [Show full text]
  • The Importance of Arginase for Vascular Dysfunction in Patients with Glucose and Lipid Abnormalities
    The Karolinska Institutet, Department of Medicine Cardiology Unit, Karolinska University Hospital Stockholm, Sweden The importance of arginase for vascular dysfunction in patients with glucose and lipid abnormalities by Oskar Kövamees Stockholm 2017 The cover figure is reprinted with permission from Shutterstock.com. All previously published papers are reproduced with permission from the publisher. Published and printed by E-print AB 2017 © Oskar Kövamees, 2017 ISBN 978-91-7676-716-0 To my family “Do not go where the path may lead, go instead where there is no path and leave a trail.” Ralph Waldo Emerton Karolinska Institutet, Department of Medicine, Cardiology Unit, Karolinska University Hospital, Stockholm, Sweden The importance of arginase for vascular dysfunction in patients with glucose and lipid abnormalities AKADEMISK AVHANDLING som för avläggande av medicine doktorsexamen vid Karolinska Institutet offentligen försvaras i Rehabsalen, Norrbacka, Karolinska Universitetssjukhuset Solna, fredagen den 9 juni 2017 kl 09.00 av Oskar Kövamees M.D. Huvudhandledare: Fakultetsopponent: Professor John Pernow Professor Per-Anders Jansson Enheten för kardiologi Avd. för molekylär och klinisk medicin Institutionen för medicin, Solna Institutionen för medicin Karolinska Institutet Sahlgrenska Akademin, Göteborgs Universitet Bihandledare: Betygsnämnd: Professor Claes-Göran Östenson Professor Lars Lind Enheten för endokrinologi och diabetes Enheten för kardiovaskulär epidemiologi Institutionen för molekylär medicin och Institutionen för medicinska
    [Show full text]
  • Role of the Nitric Oxide-Cyclic GMP Pathway in Regulation of Vaginal Blood Flow
    International Journal of Impotence Research (2003) 15, 355–361 & 2003 Nature Publishing Group All rights reserved 0955-9930/03 $25.00 www.nature.com/ijir Role of the nitric oxide-cyclic GMP pathway in regulation of vaginal blood flow SW Kim1, S-J Jeong2, R Munarriz2,NNKim2, I Goldstein2 and AM Traish2,3* 1Department of Urology, Seoul National University College of Medicine, Seoul, Korea; 2Department of Urology, Boston University School of Medicine, Boston, Massachusetts, USA; and 3Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts, USA The regulatory role of nitric oxide (NO) in vaginal perfusion remains unclear. We used specific inhibitors of enzymes in the NO-cyclic GMP (NO-cGMP) pathway and investigated their effects on vaginal blood flow in the rabbit. NO synthase (NOS) activity was similar in both the proximal and distal rabbit vagina; whereas, arginase activity was 3.4-fold higher in the distal vagina. Intravenous administration of the NOS inhibitor L-NAME resulted in a 66% reduction in genital tissue oxyhemoglobin and a 53% reduction in vaginal blood flow. This attenuation occurred despite a 20–30% increase in systemic arterial pressure. The arginase inhibitor ABH caused a 2.1-fold increase in genital tissue oxyhemoglobin and 34% increase in vaginal blood flow. The guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one and the phosphodiesterase type 5 inhibitor sildenafil caused in a 37% reduction and a 44% increase in vaginal blood flow, respectively. These observations suggest that the NO-cGMP pathway is an important regulator of vaginal hemodynamics. International Journal of Impotence Research (2003) 15, 355–361.
    [Show full text]