DOCSLIB.ORG

Candidate Enzymes for Saffron Crocin Biosynthesis Are Localized in Multiple Cellular Compartments

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Candidate Enzymes for Saffron Crocin Biosynthesis Are Localized in Multiple Cellular Compartments Candidate enzymes for saffron crocin biosynthesis are localized in multiple cellular compartments Item Type Article Authors Demurtas, Olivia Costantina; Frusciante, Sarah; Ferrante, Paola; Diretto, Gianfranco; Azad, Noraddin Hosseinpour; Pietrella, Marco; Aprea, Giuseppe; Taddei, Anna Rita; Romano, Elena; Mi, Jianing; Al-Babili, Salim; Frigerio, Lorenzo; Giuliano, Giovanni Citation Demurtas OC, Frusciante S, Ferrante P, Diretto G, Azad NH, et al. (2018) Candidate enzymes for saffron crocin biosynthesis are localized in multiple cellular compartments. Plant Physiology: pp.01815.2017. Available: http://dx.doi.org/10.1104/pp.17.01815. Eprint version Post-print DOI 10.1104/pp.17.01815 Publisher American Society of Plant Biologists (ASPB) Journal Plant Physiology Rights Archived with thanks to Plant Physiology Download date 01/10/2021 03:59:46 Link to Item http://hdl.handle.net/10754/628006 Plant Physiology Preview. Published on May 29, 2018, as DOI:10.1104/pp.17.01815 1 Short title: Compartmentation of saffron crocin biosynthesis 2 3 Corresponding author: Giovanni Giuliano, ENEA, Italian National Agency for New 4 Technologies, Energy and Sustainable Economic Development, Casaccia Research Centre, Via 5 Anguillarese n° 301, 00123 Rome, Italy; Phone: +390630483192, E-mail: 6 [email protected] 7 8 Candidate enzymes for saffron crocin biosynthesis are localized in multiple cellular 9 compartments 10 11 Olivia Costantina Demurtas1, Sarah Frusciante1, Paola Ferrante1, Gianfranco Diretto1, Noraddin 12 Hosseinpour Azad2, Marco Pietrella1,3, Giuseppe Aprea1, Anna Rita Taddei4, Elena Romano5, 13 Jianing Mi6, Salim Al-Babili6, Lorenzo Frigerio7, Giovanni Giuliano1* 14 15 1Italian National Agency for New Technologies, Energy and Sustainable Economic 16 Development (ENEA), Casaccia Res. Ctr., 00123, Rome, Italy 17 2Department of Medicinal plant and plant production, University of Mohaghegh Ardabili, 18 Ardabil, Iran 19 3Council for Agricultural Research and Economics (CREA), Research Center for Olive, Citrus 20 and Tree Fruit, 47121 Forlì, Italy 21 4Center of Large Equipment-section of Electron Microscopy, University of Tuscia, Largo 22 dell’Università, snc, Viterbo, Italy 23 5Department of Biology, University of Rome "Tor Vergata", Via della Ricerca Scientifica Snc, 24 00133 Rome, Italy. 25 6King Abdullah University of Science and Technology (KAUST), Biological and 26 Environmental Sciences and Engineering Division, The Bioactives Lab, Thuwal 23955-6900, 27 Kingdom of Saudi Arabia 28 7School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom 29 30 One-sentence summary: Carotenoid cleavage dioxygenase 2 and candidate aldehyde 31 dehydrogenase and UDP-glycosyltransferase enzymes involved in saffron crocin biosynthesis 32 are localized in the chromoplast, the endoplasmic reticulum, and the cytoplasm. 33 34 Footnotes: 1 Downloaded from on June 3, 2018 - Published by www.plantphysiol.org Copyright © 2018 American Society of Plant Biologists. All rights reserved. Copyright 2018 by the American Society of Plant Biologists 35 Author contributions: O.C.D., S.F., P.F., G.D., N.H.A., A.R.T., E.R., J.M., S.A-B., and L.F. 36 produced data. O.C.D., M.P., G.A., S.A-B., L.F., and G.G. analyzed data. G.G. coordinated the 37 study. O.C.D. and G.G. wrote the manuscript. All authors reviewed the results and approved the 38 final version of the manuscript. 39 40 Funding: This work was supported by the European Union [From discovery to products: A 41 next generation pipeline for the sustainable generation of high-value plant products, FP7 42 Contract 613153], by baseline funding given to S. A-B. from King Abdullah University of 43 Science and Technology (KAUST) and benefited from the networking activities within the 44 European Cooperation in Science and Technology Action CA15136 (EUROCAROTEN). 45 46 *Corresponding author email: [email protected] 47 48 2 Downloaded from on June 3, 2018 - Published by www.plantphysiol.org Copyright © 2018 American Society of Plant Biologists. All rights reserved. 49 Abstract 50 51 Saffron is the dried stigmas of Crocus sativus and is the most expensive spice in the world. Its 52 red color is due to crocins, which are apocarotenoid glycosides that accumulate in the vacuole to 53 a level up to 10% of the stigma dry weight. Previously, we characterized the first dedicated 54 enzyme in the crocin biosynthetic pathway, carotenoid cleavage dioxygenase 2 (CsCCD2), 55 which cleaves zeaxanthin to yield crocetin dialdehyde. In this work, we identified six putative 56 aldehyde dehydrogenase (ALDH) genes expressed in C. sativus stigmas. Heterologous 57 expression in E. coli showed that only one of corresponding proteins (CsALDH3I1) was able to 58 convert crocetin dialdehyde into the crocin precursor crocetin. CsALDH3I1 carries a C-terminal 59 hydrophobic domain, similar to that of the Neurospora membrane-associated apocarotenoid 60 dehydrogenase YLO-1. We also characterized the UDP-glycosyltransferase CsUGT74AD1, 61 which converts crocetin to crocins 1 and 2’. In vitro assays revealed high specificity of 62 CsALDH3I1 for crocetin dialdehyde and long-chain apocarotenals, and of CsUGT74AD1 for 63 crocetin. Following extract fractionation, CsCCD2, CsALDH3I1, and CsUGT74AD1 were 64 found in the insoluble fraction, suggesting their association with membranes or large insoluble 65 complexes. Analysis of protein localization in both C. sativus stigmas and following transgene 66 expression in Nicotiana benthamiana leaves revealed that CsCCD2, CsALDH3I, and 67 CsUGT74AD1 were respectively localized to the plastids, the endoplasmic reticulum (ER), and 68 the cytoplasm in association with cytoskeletal-like structures. Based on these findings and 69 current literature, we propose that the ER and cytoplasm function as “transit centers” for 70 metabolites whose biosynthesis starts in the plastid and are accumulated in the vacuole. 71 72 3 Downloaded from on June 3, 2018 - Published by www.plantphysiol.org Copyright © 2018 American Society of Plant Biologists. All rights reserved. 73 Introduction 74 One gram of dried saffron is composed of the stigmas of approximately 150 Crocus sativus 75 flowers, picked by hand. This makes saffron the most expensive spice in the world, with prices 76 ranging from 2 to 10 EUR/gram. The main organoleptic properties of the stigmas are due to the 77 accumulation of three apocarotenoid classes: crocins (composed of different glucosyl- and 78 gentiobiosyl-esters of crocetin), picrocrocin, and safranal (Tarantilis et al., 1995), conferring to 79 saffron, respectively, its red color, bitter taste, and pungent aroma. Crocins make up to 10% of 80 stigma dry weight and, by virtue of their glycosylation, are water-soluble. They have 81 applications as textile colorants and histochemical stains (Bathaie et al., 2014), antioxidants 82 (Alavizadeh and Hosseinzadeh, 2014), and in the prevention of age-related macular 83 degeneration (Bisti et al., 2014). Due to their complex structure and abundance of chiral centers, 84 crocins cannot be synthesized chemically, and there is a strong industrial interest in their 85 biotechnological production. 86 87 In C. sativus stigmas, crocin biosynthesis starts with symmetric oxidative cleavage of the C7,C8 88 and C7’,C8’ double bonds in zeaxanthin, which is catalyzed by the enzyme carotenoid cleavage 89 dioxygenase 2 (CsCCD2) producing crocetin dialdehyde (Fig. 1; Frusciante et al., 2014). Like 90 many aldehydes, crocetin dialdehyde is highly reactive and is converted into the diacid crocetin 91 by yet unidentified aldehyde dehydrogenase (ALDH) enzymes. ALDHs are NAD(P)+- 92 dependent oxidoreductases that generally contribute to different processes, such as cytoplasmic 93 male sterility, plant defense, and abiotic stress response (Kirch et al., 2004). The final step of 94 crocin biosynthesis involves glycosylation of crocetin. This reaction is usually catalyzed by 95 uridine diphosphate glycosyl transferase (UGTs) that mediate the glycosylation of secondary 96 metabolites, xenobiotics, and hormones (Wang, 2009). In Gardenia jasminoides, two crocetin 97 UGTs have been characterized (Nagatoshi et al., 2012): UGT75L6 is responsible for the 98 primary glycosylation of crocetin, producing crocetin monoglucosyl and diglucosyl esters, 99 whereas UGT94E5 is responsible for the secondary glycosylation of the glucose groups, leading 100 to the formation of one or two gentiobiose groups (Fig. 1). Contrasting data have been reported 101 on crocin formation in saffron: a crocetin UGT purified from C. sativus stigmas has been shown 102 to catalyze only primary glycosylation (Cote et al., 2001) whereas a cloned UGT (UGTCs2) has 103 been reported to mediate both types of glycosylation (Moraga et al., 2004). 104 105 The crocin biosynthetic pathway encompasses multiple subcellular compartments: the initial 106 precursor, zeaxanthin, is localized in plastids whereas the final products, crocins, accumulate in 107 vacuoles (Bouvier et al., 2003; Gomez-Gomez et al., 2017), like many other plant glycosylated 4 Downloaded from on June 3, 2018 - Published by www.plantphysiol.org Copyright © 2018 American Society of Plant Biologists. All rights reserved. 108 metabolites (Martinoia et al., 2007). We have identified the carotenoid cleavage dioxygenase 109 responsible for the first dedicated step in C. sativus carotenoid biosynthesis, CsCCD2. It has 110 been shown that CsCCD2, the enzyme that catalyzes the cleavage of zeaxanthin in crocin 111 biosynthesis (Frusciante et al., 2014),
Load more

Recommended publications
  • Function and Mechanism of WRKY Transcription Factors in Abiotic Stress Responses of Plants
    plants Review Function and Mechanism of WRKY Transcription Factors in Abiotic Stress Responses of Plants Weixing Li y , Siyu Pang y, Zhaogeng Lu and Biao Jin * College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; [email protected] (W.L.); [email protected] (S.P.); [email protected] (Z.L.) * Correspondence: [email protected] Contributed equally to this work. y Received: 26 September 2020; Accepted: 4 November 2020; Published: 8 November 2020 Abstract: The WRKY gene family is a plant-specific transcription factor (TF) group, playing important roles in many different response pathways of diverse abiotic stresses (drought, saline, alkali, temperature, and ultraviolet radiation, and so forth). In recent years, many studies have explored the role and mechanism of WRKY family members from model plants to agricultural crops and other species. Abiotic stress adversely affects the growth and development of plants. Thus, a review of WRKY with stress responses is important to increase our understanding of abiotic stress responses in plants. Here, we summarize the structural characteristics and regulatory mechanism of WRKY transcription factors and their responses to abiotic stress. We also discuss current issues and future perspectives of WRKY transcription factor research. Keywords: WRKY transcription factor; abiotic stress; gene structural characteristics; regulatory mechanism; drought; salinity; heat; cold; ultraviolet radiation 1. Introduction As a fixed-growth organism, plants are exposed to a variety of environmental conditions and may encounter many abiotic stresses, for example, drought, waterlogging, heat, cold, salinity, and Ultraviolet-B (UV-B) radiation. To adapt and counteract the effects of such abiotic stresses, plants have evolved several molecular mechanisms involving signal transduction and gene expression [1,2].
    [Show full text]
  • S-Abscisic Acid
    CLH REPORT FOR[S-(Z,E)]-5-(1-HYDROXY-2,6,6-TRIMETHYL-4-OXOCYCLOHEX-2-EN- 1-YL)-3-METHYLPENTA-2,4-DIENOIC ACID; S-ABSCISIC ACID CLH report Proposal for Harmonised Classification and Labelling Based on Regulation (EC) No 1272/2008 (CLP Regulation), Annex VI, Part 2 International Chemical Identification: [S-(Z,E)]-5-(1-hydroxy-2,6,6-trimethyl-4-oxocyclohex-2- en-1-yl)-3-methylpenta-2,4-dienoic acid; S-abscisic acid EC Number: 244-319-5 CAS Number: 21293-29-8 Index Number: - Contact details for dossier submitter: Bureau REACH National Institute for Public Health and the Environment (RIVM) The Netherlands [email protected] Version number: 1 Date: August 2018 Note on confidential information Please be aware that this report is intended to be made publicly available. Therefore it should not contain any confidential information. Such information should be provided in a separate confidential Annex to this report, clearly marked as such. [04.01-MF-003.01] CLH REPORT FOR[S-(Z,E)]-5-(1-HYDROXY-2,6,6-TRIMETHYL-4-OXOCYCLOHEX-2-EN- 1-YL)-3-METHYLPENTA-2,4-DIENOIC ACID; S-ABSCISIC ACID CONTENTS 1 IDENTITY OF THE SUBSTANCE........................................................................................................................1 1.1 NAME AND OTHER IDENTIFIERS OF THE SUBSTANCE...............................................................................................1 1.2 COMPOSITION OF THE SUBSTANCE..........................................................................................................................1 2 PROPOSED HARMONISED
    [Show full text]
  • Biologically Active Compounds in Seaweed Extracts Useful in Animal Diet
    20 The Open Conference Proceedings Journal, 2012, 3, (Suppl 1-M4) 20-28 Open Access Biologically Active Compounds in Seaweed Extracts - the Prospects for the Application Katarzyna Chojnacka*, Agnieszka Saeid, Zuzanna Witkowska and Łukasz Tuhy Institute of Inorganic Technology and Mineral Fertilizers, Wroclaw University of Technology ul. Smoluchowskiego 25, 50-372 Wroclaw, Poland Abstract: The paper covers the latest developments in research on the utilitarian properties of algal extracts. Their appli- cation as the components of pharmaceuticals, feeds for animals and fertilizers was discussed. The classes of various bio- logically active compounds were characterized in terms of their role and the mechanism of action in an organism of hu- man, animal and plant. Recently, many papers have been published which discuss the methods of manufacture and the composition of algal ex- tracts. The general conclusion is that the composition of extracts strongly depends on the raw material (geographical loca- tion of harvested algae and algal species) as well as on the extraction method. The biologically active compounds which are transferred from the biomass of algae to the liquid phase include polysaccharides, proteins, polyunsaturated fatty ac- ids, pigments, polyphenols, minerals, plant growth hormones and other. They have well documented beneficial effect on humans, animals and plants, mainly by protection of an organism from biotic and abiotic stress (antibacterial activity, scavenging of free radicals, host defense activity etc.) and can be valuable components of pharmaceuticals, feed additives and fertilizers. Keywords: Algal extracts, feed additives, fertilizers, pharmaceuticals, biologically active compounds. 1. INTRODUCTION was paid to biologically active compounds, useful as the components of pharmaceuticals, feeds and fertilizers.
    [Show full text]
  • Abscisic Acid Is Involved in the Wound-Induced Expression of the Proteinase Inhibitor II Gene in Potato and Tomato HUGO PENA-CORTIS*T, Jose J
    Proc. Nadl. Acad. Sci. USA Vol. 86, pp. 9851-9855, December 1989 Botany Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato HUGO PENA-CORTIS*t, Jose J. SANCHEZ-SERRANO*, RUDIGER MERTENSt, LOTHAR WILLMITZER*, AND SALOME' PRAT* *Institut fur Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000, Berlin 33, Federal Republic of Germany; and tSchering AG, Gollanczstrasse 57-101, D-1000, Berlin 28, Federal Republic of Germany Communicated by J. Schell, August 9, 1989 ABSTRACT Plants respond to wounding or pathogen at- abscisic acid (ABA) have been reported as a result of water tack by a variety of biochemical reactions, involving in some or osmotic stress conditions (8, 9), whereas ethylene biosyn- instances gene activation in tissues far apart from the actual site thesis has been associated with the initial response of the of wounding or pathogen invasion. One of the best analyzed plant tissue to mechanical wounding (10). Indirect evidence examples for such a systemic reaction is the wound-induced for the involvement of ABA in wound responses has also expression of proteinase inhibitor genes in tomato and potato been obtained from two maize proteins, whose synthesis is leaves. Local wounding ofpotato or tomato plants results in the induced by water stress and by ABA and in addition shows accumulation of proteinase inhibitors I and II throughout the low wound inducibility (11, 12). aerial part of the plant. In contrast to wild-type plants, abscisic We, therefore, decided to test whether or not ABA is acid-deficient mutants ofpotato (droopy) and tomato (sit) show involved in the systemic induction of the PI-II gene.
    [Show full text]
  • Functions of Jasmonic Acid in Plant Regulation and Response to Abiotic Stress
    International Journal of Molecular Sciences Review Functions of Jasmonic Acid in Plant Regulation and Response to Abiotic Stress Jia Wang 1 , Li Song 1, Xue Gong 1, Jinfan Xu 1 and Minhui Li 1,2,3,* 1 Inner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization, Baotou Medical College, Baotou 014060, China; [email protected] (J.W.); [email protected] (L.S.); [email protected] (X.G.); [email protected] (J.X.) 2 Pharmaceutical Laboratory, Inner Mongolia Institute of Traditional Chinese Medicine, Hohhot 010020, China 3 Qiqihar Medical University, Qiqihar 161006, China * Correspondence: [email protected]; Tel.: +86-4727-1677-95 Received: 29 December 2019; Accepted: 18 February 2020; Published: 20 February 2020 Abstract: Jasmonic acid (JA) is an endogenous growth-regulating substance, initially identified as a stress-related hormone in higher plants. Similarly, the exogenous application of JA also has a regulatory effect on plants. Abiotic stress often causes large-scale plant damage. In this review, we focus on the JA signaling pathways in response to abiotic stresses, including cold, drought, salinity, heavy metals, and light. On the other hand, JA does not play an independent regulatory role, but works in a complex signal network with other phytohormone signaling pathways. In this review, we will discuss transcription factors and genes involved in the regulation of the JA signaling pathway in response to abiotic stress. In this process, the JAZ-MYC module plays a central role in the JA signaling pathway through integration of regulatory transcription factors and related genes. Simultaneously, JA has synergistic and antagonistic effects with abscisic acid (ABA), ethylene (ET), salicylic acid (SA), and other plant hormones in the process of resisting environmental stress.
    [Show full text]
  • Abscisic Acid Mediates Drought and Salt Stress Responses in Vitis Vinifera—A Review
    International Journal of Molecular Sciences Review Abscisic Acid Mediates Drought and Salt Stress Responses in Vitis vinifera—A Review Daniel Marusig and Sergio Tombesi * Dipartimento di Scienze delle Produzioni Vegetali Sostenibili, Università Cattolica del Sacro Cuore, 29122 Piacenza, Italy; [email protected] * Correspondence: [email protected]; Tel.: +39-0523-599221 Received: 21 October 2020; Accepted: 15 November 2020; Published: 17 November 2020 Abstract: The foreseen increase in evaporative demand and reduction in rainfall occurrence are expected to stress the abiotic constrains of drought and salt concentration in soil. The intensification of abiotic stresses coupled with the progressive depletion in water pools is a major concern especially in viticulture, as most vineyards rely on water provided by rainfall. Because its economical relevance and its use as a model species for the study of abiotic stress effect on perennial plants, a significant amount of literature has focused on Vitis vinifera, assessing the physiological mechanisms occurring under stress. Despite the complexity of the stress-resistance strategy of grapevine, the ensemble of phenomena involved seems to be regulated by the key hormone abscisic acid (ABA). This review aims at summarizing our knowledge on the role of ABA in mediating mechanisms whereby grapevine copes with abiotic stresses and to highlight aspects that deserve more attention in future research. Keywords: ABA; grapevine; stomata; drought; metabolism; carbohydrates; salinity 1. Introduction Climate change is expected to have negative impacts on the socioeconomic system [1]. Despite the discrepancy between the projected scenarios, even the most optimistic models foresee an increase in the occurrence and duration of anomalous droughts, especially in the Mediterranean-climate regions, where water sources will be increasingly scarce [2].
    [Show full text]
  • On the Biosynthesis and Evolution of Apocarotenoid Plant Growth Regulators
    On the biosynthesis and evolution of apocarotenoid plant growth regulators. Item Type Article Authors Wang, Jian You; Lin, Pei-Yu; Al-Babili, Salim Citation Wang, J. Y., Lin, P.-Y., & Al-Babili, S. (2020). On the biosynthesis and evolution of apocarotenoid plant growth regulators. Seminars in Cell & Developmental Biology. doi:10.1016/ j.semcdb.2020.07.007 Eprint version Post-print DOI 10.1016/j.semcdb.2020.07.007 Publisher Elsevier BV Journal Seminars in cell & developmental biology Rights NOTICE: this is the author’s version of a work that was accepted for publication in Seminars in cell & developmental biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Seminars in cell & developmental biology, [, , (2020-08-01)] DOI: 10.1016/j.semcdb.2020.07.007 . © 2020. This manuscript version is made available under the CC- BY-NC-ND 4.0 license http://creativecommons.org/licenses/by- nc-nd/4.0/ Download date 27/09/2021 08:08:14 Link to Item http://hdl.handle.net/10754/664532 1 On the Biosynthesis and Evolution of Apocarotenoid Plant Growth Regulators 2 Jian You Wanga,1, Pei-Yu Lina,1 and Salim Al-Babilia,* 3 Affiliations: 4 a The BioActives Lab, Center for Desert Agriculture (CDA), Biological and Environment Science 5 and Engineering (BESE), King Abdullah University of Science and Technology, Thuwal, Saudi 6 Arabia.
    [Show full text]
  • The Effect of Abscisic Acid Chronic Treatment on Neuroinflammatory
    Sánchez-Sarasúa et al. Nutrition & Metabolism (2016) 13:73 DOI 10.1186/s12986-016-0137-3 RESEARCH Open Access The effect of abscisic acid chronic treatment on neuroinflammatory markers and memory in a rat model of high-fat diet induced neuroinflammation Sandra Sánchez-Sarasúa1†, Salma Moustafa1†, Álvaro García-Avilés1, María Fernanda López-Climent2, Aurelio Gómez-Cadenas2, Francisco E. Olucha-Bordonau1 and Ana M. Sánchez-Pérez1* Abstract Background: Western diet and lifestyle are associated with overweight, obesity, and type 2 diabetes, which, in turn, are correlated with neuroinflammation processes. Exercise and a healthy diet are important in the prevention of these disorders. However, molecules inhibiting neuroinflammation might also be efficacious in the prevention and/or treatment of neurological disorders of inflammatory etiology. The abscisic acid (ABA) is a phytohormone involved in hydric-stress responses. This compound is not only found in plants but also in other organisms, including mammals. In rodents, ABA can play a beneficial role in the regulation of peripheral immune response and insulin action. Thus, we hypothesized that chronic ABA administration might exert a protective effect in a model of neuroinflammation induced by high-fat diet (HFD). Methods: Male Wistar rats were fed with standard diet or HFD with or without ABA in the drinking water for 12 weeks. Glucose tolerance test and behavioral paradigms were performed to evaluate the peripheral and central effects of treatments. One-Way ANOVA was performed analyzed statistical differences between groups. Results: The HFD induced insulin resistance peripherally and increased the levels of proinflammatory markers in in the brain. We observed that ABA restored glucose tolerance in HFD-fed rats, as expected.
    [Show full text]
  • Balancing Trade-Offs Between Biotic and Abiotic Stress Responses Through Leaf Age-Dependent Variation in Stress Hormone Cross-Talk
    Balancing trade-offs between biotic and abiotic stress responses through leaf age-dependent variation in stress hormone cross-talk Matthias L. Berensa, Katarzyna W. Wolinskaa, Stijn Spaepena, Jörg Zieglerb, Tatsuya Noboria, Aswin Naira,1, Verena Krülera,2, Thomas M. Winkelmüllera, Yiming Wanga, Akira Minea,3, Dieter Beckera, Ruben Garrido-Otera,c, Paul Schulze-Leferta,c,4, and Kenichi Tsudaa,4 aDepartment of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; bDepartment of Molecular Signal Processing, Leibnitz Institute of Plant Biochemistry, 06120 Halle, Germany; and cCluster of Excellence on Plant Sciences, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany Contributed by Paul Schulze-Lefert, December 10, 2018 (sent for review October 8, 2018; reviewed by Xinnian Dong and Murray R. Grant) In nature, plants must respond to multiple stresses simultaneously, microbiota (7), which contribute to plant performance under which likely demands cross-talk between stress-response pathways adverse environmental conditions (8–11). SA modulates coloni- to minimize fitness costs. Here we provide genetic evidence that zation of the A. thaliana root microbiota by specific bacterial biotic and abiotic stress responses are differentially prioritized in taxa, although this was not accompanied by a detectable impact Arabidopsis thaliana leaves of different ages to maintain growth on host survival of the tested SA biosynthesis and signaling and reproduction under combined biotic and
    [Show full text]
  • Signaling Crosstalk Between Salicylic Acid and Ethylene/Jasmonate in Plant Defense: Do We Understand What They Are Whispering?
    International Journal of Molecular Sciences Review Signaling Crosstalk between Salicylic Acid and Ethylene/Jasmonate in Plant Defense: Do We Understand What They Are Whispering? Ning Li 1,†, Xiao Han 2,3,†, Dan Feng 2,3,†, Deyi Yuan 1 and Li-Jun Huang 1,* 1 State Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China; [email protected] (N.L.); [email protected] (D.Y.) 2 College of Biological Science and Engineering, Fuzhou University, Fuzhou 350116, China; [email protected] (X.H.); [email protected] (D.F.) 3 Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China * Correspondence: [email protected]; Tel./Fax: +86-0731-8562-3406 † These authors contributed equality to this work. Received: 9 January 2019; Accepted: 2 February 2019; Published: 4 February 2019 Abstract: During their lifetime, plants encounter numerous biotic and abiotic stresses with diverse modes of attack. Phytohormones, including salicylic acid (SA), ethylene (ET), jasmonate (JA), abscisic acid (ABA), auxin (AUX), brassinosteroid (BR), gibberellic acid (GA), cytokinin (CK) and the recently identified strigolactones (SLs), orchestrate effective defense responses by activating defense gene expression. Genetic analysis of the model plant Arabidopsis thaliana has advanced our understanding of the function of these hormones. The SA- and ET/JA-mediated signaling pathways were thought to be the backbone of plant immune responses against biotic invaders, whereas ABA, auxin, BR, GA, CK and SL were considered to be involved in the plant immune response through modulating the SA-ET/JA signaling pathways.
    [Show full text]
  • ENDOGENOUS QUANTIFICATION of ABSCISIC ACID and INDOLE-3- ACETIC ACID in SOMATIC and ZIGOTIC EMBRYOS of Nothofagus Alpina (Poepp
    RESEARCH ENDOGENOUS QUANTIFICATION OF ABSCISIC ACID AND INDOLE-3- ACETIC ACID IN SOMATIC AND ZIGOTIC EMBRYOS OF Nothofagus alpina (Poepp. & Endl.) Oerst. Pricila Cartes Riquelme1*, Darcy Ríos Leal1, Kátia Sáez Carrillo2, Matilde Uribe Moraga1, Sofía Valenzuela Aguilar1, Stephen Joseph Bolus3, and Manuel Sánchez Olate1 Abscisic acid (ABA) and indole-3-acetic acid (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation of the embryo. The goal of the assay was to compare endogenous levels of ABA and IAA between somatic embryos (SE) and zygotic embryos (ZE) of Nothofagus alpina (Poepp. & Endl.) Oerst. In this study, a somatic embryo maturation assay involving the addition of varying concentrations of exogenous ABA was performed on cotyledonary-stage of N. alpina. Furthermore, the endogenous levels of ABA and IAA were quantified in the immature ZE, the mature ZE, and the embryonic axis of a mature embryo of N. alpina. The current study utilized high performance liquid chromatography (HPLC) for quantification. The maturation treatments performed did not present significant differences in the endogenous ABA levels in SE. However, significant differences did exist in levels of ABA and IAA between SE submitted to the different maturation treatments and mature ZE of N. alpina. The application of exogenous ABA to the culture medium increased endogenous ABA levels, therefore, increasing the number of germinated somatic embryos. Thus, the plant conversion process was also successfully completed in somatic embryos of N. alpina. Key words: Somatic embryogenesis, maturation, germination, HPLC. othofagus alpina (Poepp. & Endl.) Oerst., “raulí”, of the donor plants are “reprogrammed.” These cells is a native species of the Chilean forest.
    [Show full text]
  • Apocarotenoids: a Brief Review
    International Journal of Research and Review Vol.7; Issue: 12; December 2020 Website: www.ijrrjournal.com Review Article E-ISSN: 2349-9788; P-ISSN: 2454-2237 Apocarotenoids: A Brief Review Seba M C, Anatt Treesa Mathew, Sheeja Rekha, Prasobh G R Department of Pharmaceutical Chemistry, Sree Krishna College of Pharmacy and Research Centre, Parassala, Kerala Corresponding Author: Seba M C ABSTRACT The development of apocarotenoids is started by oxidative cleavage reactions Carotenoids are broad group of natural that can be catalyzed by enzymes, generally pigments, extending from red to orange, to CCDs, or take place via exposure of yellow colours. Produced by plants and certain carotenoids to ROS. β-Cyclocitral is an microorganisms (e.g.: fungi, bacteria and example for a carotenoid cleavage product microalgae), carotenoids have significant physiological function (e.g.: light harvesting). that can be produced following both Apocarotenoids are organic compounds, which scenarios. The formation of this β-carotene– is cleavage products of C40 isoprenoid derived volatile in photosynthetic tissues is 1 pigments, named carotenoids, catalyzed by initiated by a singlet oxygen O2 attack, enzyme carotenoid oxygenase, produced which takes place in the photosystem II, absolutely by plants and microorganisms. particularly in the presence of high light Apocarotenoids show vital roles in several stress, while in citrus fruits, it is catalyzed biological activities (e.g., plant hormones). by a CCD (CCD4b) that cleaves β-carotene, Various carotenoids and apocarotenoids have β-cryptoxanthin, and zeaxanthin. Although great economic significance in feed, cosmetics, some CCDs display a quite relaxed substrate food supplements, pharmaceutical industries and and site (double bond) specificity , cleavage also possess high commercial values Key Words: Carotenoids, Apocarotenoids, reactions catalyzed by these enzymes Carotenoid oxygenase generally take place at definite double bond(s) in defined carotenoid/apocarotenoid INTRODUCTION substrate(s).
    [Show full text]