Transcription of the Arabidopsis CPD Gene, Encoding a Steroidogenic Cytochrome P450, Is Negatively Controlled by Brassinosteroids
The Plant Journal (1998) 14(5), 593–602 Transcription of the Arabidopsis CPD gene, encoding a steroidogenic cytochrome P450, is negatively controlled by brassinosteroids Jaideep Mathur1,†, Gergely Molna´ r2, Shozo Fujioka3, protein synthesis inhibitor cycloheximide, indicating a Suguru Takatsuto4, Akira Sakurai3, Takao Yokota5, requirement for de novo synthesis of a regulatory factor. Gu¨ nter Adam6, Brunhilde Voigt6, Ferenc Nagy2, Christoph Maas7, Jeff Schell1, Csaba Koncz1,2 and 2,* Miklo´ s Szekeres Introduction 1Max Planck-Institut fu¨ rZu¨chtungsforschung, Carl-von-Linne´ -Weg 10, D-50829 Ko¨ ln, Germany, Steroid hormones play an important role in the regulation 2Institute of Plant Biology, Biological Research Center of of differentiation, sex determination, and maintenance of the Hungarian Academy of Sciences, PO Box 521, body homeostasis in animals. The biosynthesis of animal H-6701 Szeged, Hungary, steroid hormones requires at least six different cyto- 3The Institute of Physical and Chemical Research chrome P450 genes, the expression of which is tightly (RIKEN), Wako-shi, Saitama 351–01, Japan, regulated by signalling mechanisms safeguarding choles- 4Department of Chemistry, Joetsu University of terol homeostasis (Honda et al., 1993; Waterman and Education, Joetsu-shi, Niigata 943, Japan, Bischof, 1997). High cholesterol levels in animals enhance 5Department of Biosciences, Teikyo University, the synthesis of oxidized cholesterol derivatives termed Utsunomiya 320, Japan, oxysterols. In turn, oxysterols induce an end-product 6Department of Natural Products, Institute of Plant repression of genes involved in steroid biosynthesis Biochemistry, Weinberg 3, PO Box 100432, D-06018 Halle, (Brown and Goldstein, 1997; Goldstein and Brown, 1990). Germany, and Recently, genetic analysis of Arabidopsis and garden pea mutants has provided unequivocal evidence that brassino- 7Hoeschst Schering AgrEvo GmbH, Ho¨ chst Works H872N, D-65926 Frankfurt am Main, Germany steroids (BRs) are essential phytohormones (Clouse, 1996; Hooley, 1996).
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