Home Search Collections Journals About Contact us My IOPscience Simultaneous measurement of rotational and translational diffusion of anisotropic colloids with a new integrated setup for fluorescence recovery after photobleaching This article has been downloaded from IOPscience. Please scroll down to see the full text article. 2012 J. Phys.: Condens. Matter 24 245101 (http://iopscience.iop.org/0953-8984/24/24/245101) View the table of contents for this issue, or go to the journal homepage for more Download details: IP Address: 131.211.152.249 The article was downloaded on 22/05/2012 at 11:06 Please note that terms and conditions apply. IOP PUBLISHING JOURNAL OF PHYSICS: CONDENSED MATTER J. Phys.: Condens. Matter 24 (2012) 245101 (15pp) doi:10.1088/0953-8984/24/24/245101 Simultaneous measurement of rotational and translational diffusion of anisotropic colloids with a new integrated setup for fluorescence recovery after photobleaching B W M Kuipers, M C A van de Ven, R J Baars and A P Philipse Van’t Hoff Laboratory for Physical and Colloid Chemistry, Debye Institute for Nanomaterials Science, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands E-mail:
[email protected] Received 20 February 2012, in final form 10 April 2012 Published 8 May 2012 Online at stacks.iop.org/JPhysCM/24/245101 Abstract This paper describes an integrated setup for fluorescence recovery after photobleaching (FRAP) for determining translational and rotational Brownian diffusion simultaneously, ensuring that these two quantities are measured under exactly the same conditions and at the same time in dynamic experiments. The setup is based on translational-FRAP with a fringe pattern of light for both the bleaching and monitoring of fluorescently labeled particles, and rotational-FRAP, which uses the polarization of a short bleach light pulse to create a polarization anisotropy.