Bead Size Recommendation Guide Pluribead®

Bead Size Recommendation Guide pluriBead® pluriBead® is available in two different sizes: S-pluriBead® (4 mL) or Whole Blood Buffy Coat PBMC / Cell M-pluriBead® (10 mL). Each is differently efficient. If you are not sure Culture wether to use S-pluriBead® or M-pluriBead ®- this guide shows our recommendation. Recommendation Recommendation Recommendation

Marker Cell Types S M S M S M Human CD2 T cells, NK cell subset ✓ ✓ ✓ CD3 T cells ✓ ✓ ✓ CD4 Helper T cells ✓ ✓ ✓ CD5 T cells, B cells subset ✓ ✓ ✓ CD7 T cells, NK cells, pre-B cells ✓ ✓ ✓ CD8 Cytotoxic T cells ✓ ✓ ✓ CD9 Platelets, granulocytes ✓ ✓ ✓ CD11a Leukocytes ✓ ✓ ✓ CD11b Granulocytes, monocytes, macrophages ✓ ✓ ✓ CD14 Monocytes ✓ ✓ ✓ CD15 Granulocytes ✓ ✓ ✓ CD16 Neutrophils, granulocytes ✓ ✓ ✓ CD19 B cells ✓ ✓ ✓ CD21 B cells, T cell subset ✓ ✓ ✓ CD31 Platelets, endothelial cells ✓ ✓ ✓ CD43 T cells, NK cells, granulocytes ✓ ✓ ✓ CD44 Most tissue cells ✓ ✓ ✓ CD45 Leukocytes ✓ ✓ ✓ CD55 Hematopoietic cells ✓ ✓ ✓ CD95 Apoptotic cells ✓ ✓ ✓ CD117 Hematopoietic progenitor cells (c-Kit+) ✓ ✓ ✓ CD138 Plasma cells ✓ ✓ ✓ CD235a Erythrocytes (RBC) ✓ ✓ ✓ CD271 Mesemchymal stem/stromal cells, Schwann cells ✓ ✓ ✓ CD326 Epithelial Cells, circulating tumor cells ✓ ✓ ✓ Murine CD4 Helper T cells ✓ N/A N/A ✓ CD8 Cytotoxic T cells ✓ N/A N/A ✓ CD11b Monocytes, macrophages, NK cells, granulocytes ✓ N/A N/A ✓ CD11c Dendritic cells, activated T cells ✓ N/A N/A ✓ CD14 Monocytes ✓ N/A N/A ✓ CD19 B cells ✓ N/A N/A ✓ CD31 Platelets, endothelial cells ✓ N/A N/A ✓ CD45 Leukocytes ✓ N/A N/A ✓ F4/80 Macrophages ✓ N/A N/A ✓ Ly6G Granulocytes ✓ N/A N/A ✓ Universal Label your own specific antibody ✓ ✓ ✓

* rare cells strongly dependend on the number of target cells in the sample material ** cross reaction with platelets in this sample material! N/A - not available (sample material)

T Cell B Cell

CD3 CD19 CD4 (Helper T cell) CD8 (Cytotoxic T cell)

Monocyte/Macrophage Granulocyte

CD14 CD15 CD33 CD16 (Neutrophile) Ly6G (Murine) F4/80 (Murine)

Dentritic Cell Platelet

CD11c CD9 (Whole blood) CD86 CD41 CD61

Stem Cell Erythrocyte

CD117 (Hematopoietic progenitor cell) CD235a CD271 (Mesenchymal stem/stromal cell)

Endothelial Cell Epithelial Cell

CD31 CD326

pluriBead® is a unique cell separation technology that works without any magnetic components. The proce- dure is simple:

You sieve your pluriBeads (with bound target cells) down a strainer - the pluriBeads with your target cells stay on top, the unwanted cells run through. After de- taching, you have your target cells ready.