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An Entomopathogenic Caenorhabditis Briggsae 3223 The Journal of Experimental Biology 213, 3223-3229 © 2010. Published by The Company of Biologists Ltd doi:10.1242/jeb.043109 An entomopathogenic Caenorhabditis briggsae Eyualem Abebe1,*, Miriam Jumba2, Kaitlin Bonner3, Vince Gray2, Krystalynne Morris3 and W. Kelley Thomas3 1Department of Biology, Elizabeth City State University, 1704 Weeksville Road, Jenkins Science Center 421, Elizabeth City, NC 27909, USA, 2School of Molecular and Cell Biology, University of the Witwatersrand, Republic of South Africa and 3Hubbard Center for Genome Studies, University of New Hampshire, 35 Colovos Road, Durham, NH 03824, USA *Author for correspondence ([email protected]) Accepted 27 May 2010 SUMMARY Caenorhabditis elegans is a premier model organism upon which considerable knowledge of basic cell and developmental biology has been built. Yet, as is true for many traditional model systems, we have limited knowledge of the ecological context in which these systems evolved, severely limiting our understanding of gene function. A better grasp of the ecology of model systems would help us immensely in understanding the functionality of genes and evolution of genomes in an environmental context. Consequently, there are ongoing efforts to uncover natural populations of this model system globally. Here, we describe the discovery of a Caenorhabditis briggsae strain and its bacterial associate (Serratia sp.) that form an entomopathogenic complex in the wild. Laboratory experiments confirm that this nematode and its natural bacterial associate can penetrate, kill and reproduce in an insect host and that the bacterial associate can induce this insect pathogenic life cycle in other Caenorhabditis species, including C. elegans. Our findings suggest that this life history may be widespread in nature and critical to the understanding of the biology of this important model organism. Caenorhabditis–insect interaction could be a key factor in our quest for a better grasp of gene functionality in this important model species. The discovered association, consequently, would provide an ecological framework for functional genomics of Caenorhabditis. Supplementary material available online at http://jeb.biologists.org/cgi/content/full/213/18/3223/DC1 Key words: insect parasite, nematode, symbiosis, nematode–bacterial association, African Caenorhabditis briggsae. INTRODUCTION animals, most often invertebrates (Caswell-Chen et al., 2005), in Linking the complete genome sequences of an organism to its either a phoretic relationship travelling on the host between food development and behaviour remains a major goal in biology sources, or a necromenic relationship where the nematode waits for (Kamath et al., 2003). Progress towards that goal has traditionally the demise of its ‘host’ and feeds on the resulting decay (Kiontke relied on model organisms such as the nematode Caenorhabditis and Sudhaus, 2006). In both cases, it is the dauer or resistant larval elegans upon which considerable knowledge of cell and stage that is associated with the host. Phoretic and necromenic developmental biology has been built (Wood, 1988) (Wormbook, associations are two of many known associations between http://www.wormbook.org). Interest in the development of nematodes and other animals. Hitherto, none of the known alternative models for understanding host–pathogen interactions has Caenorhabditis are reported to be entomopathogenic. resulted in the use of Caenorhabditis and a variety of Among the most interesting associations within the phylum microorganisms (Couillault and Ewbank, 2002; Schulenburg and Nematoda are those of the entomopathogenic nematodes (EPNs). Ewbank, 2004; Pradel et al., 2007; Kim, 2008). Unfortunately, C. EPNs represent the collaboration between a nematode and bacterium elegans, like most model organisms, was chosen for its laboratory where, as in necromenia, the nematode feeds on bacteria using the prowess (e.g. short generation time, tractable development), and not insect as a carbon source (Burnell and Stock, 2000; Rae et al., 2008); because we had a detailed understanding of its ecology (Wood, 1988; however, in a bacterial/EPN complex, the bacteria actively kills the Feder and Mitchell-Olds, 2003; Kinotke and Sudhaus, 2006). insect after gaining access with the aid of the nematode partner. Consequently, as our catalogue of genes and the tools to explore Among species of the genus Caenorhabditis, reports show that C. function for organisms like C. elegans has become extraordinarily briggsae is necromenic, C. japonica is a facultative necromenic, rich, the absence of an ecological context has become a major and C. elegans and C. remanei are primarily phoretic with some limitation to understanding biological function (Caswell-Chen et al., questionable necromenic associations (Kiontke and Sudhaus, 2006). 2005). Sudhaus (Sudhaus, 1993) suggested the two well-known EPN A concerted effort is now underway to obtain a greater genera, i.e. Heterorhabditis and Steinernema, most probably evolved understanding of C. elegans outside of the laboratory including the from necromenic nematodes through the association with an first studies focusing on genetic diversity in natural populations of entomopathogenic bacterium. C. elegans and related species (Barrière and Félix, 2005; Fitch, 2005; Entomopathogenesis appears to have evolved at least twice in Sivasundar and Hey, 2005; Cutter et al., 2006; Haag et al., 2007). nematodes (Poinar, 1993; Blaxter et al., 1998): once in the lineage Rather than being a truly free-living soil nematode as it is often giving rise to the Heterorhabditis and its bacterial associate portrayed, Caenorhabditis is thought to be associated with other Photorhabdus and once in the lineage leading to the Steinernematidae THE JOURNAL OF EXPERIMENTAL BIOLOGY 3224 E. Abebe and others (Steinernema) and its bacterial associate Xenorhabdus. These EPNs mellonella to reflect possible natural conditions. The two other have broad insect host ranges, and are used as agents of biological cultures were reared under normal C. elegans laboratory conditions control and to study the evolution of the mutualistic relationships using nematode growth media (NGM) agar with one set seeded between nematodes and bacteria (Adams et al., 2006). The collection with E. coli OP50 and the other set seeded with the isolated of EPNs from nature is traditionally by the use of Galleria traps, associated bacterium, Serratia sp. SCBI (South African which are wax moth larvae (caterpillars) buried in the soil (Bedding Caenorhabditis briggsae isolate). The culture maintained on E. and Akhurst, 1975). The Galleria cadavers are collected and coli OP50 was initially bleached twice following a C. elegans nematodes (virtually always either Steinernematidae or culture contaminant cleaning protocol (Stiernagle, 2006) to Heterorhabditis) are collected. The EPN families have global eliminate its bacterial associate – Serratia sp. SCBI. Frozen stock distributions with apparent co-evolution of nematode and bacterium cultures of nematode and associated bacterium in glycerol stored (Adams et al., 2007). These nematodes can routinely be cultured on at –80°C were established and are maintained at the Hubbard agar plates with bacteria as a food source but in nature are considered Center for Genome Studies at the University of New Hampshire. to always be involved in entomopathogenic life cycles. In Galleria trap experiments conducted globally, only rarely are other nematodes Establishing nematode identity reported (Rueda et al., 1993; Ye et al., 2010). Recently, Galleria We studied nematodes morphologically at 1000ϫ magnification trap experiments and direct isolation methods have, however, using an Olympus IX81 inverted compound microscope with a revealed nematodes other than members of the Steinernematidae and differential interference contrast option. We also studied nematodes Heterorhabditis (Young-Keun et al., 2007; Zhang et al., 2008). using an Amray 3300FE field emission scanning electron Moreover, Zhang and colleagues (Zhang et al., 2009) reported a new microscope (SEM) with PGT Imix-PC microanalysis system to species of Serratia that they found symbiotically associated with a evaluate whether bacteria were attached to the nematode cuticle new entomopathogenic nematode genus discovered earlier (Zhang surface externally. et al., 2008). Partial DNA sequences of the small and large subunit ribosomal Here we report our findings, an entomopathogenic genes, internal transcribed spacer and mitochondrial genes Caenorhabditis and its bacterial associate, from a recent Galleria cytochrome oxidase II (COII) and NADH dehydrogenase subunit trapping experiment that included several independent Galleria traps 5 (ND5) were amplified and sequenced from the nematode strain in soils from three provinces (North West, Mpumalanga and Kwa using the primers in supplementary material TableS1. Once the Zulu-Natal) in South Africa. species level identity of the strain was established as C. briggsae (Dougherty and Nigon 1949), we conducted further comparisons MATERIALS AND METHODS between our strain, i.e. C. briggsae KT0001, and 10 other wild- Isolation of nematodes and their bacterial associate type isolates of C. briggsae obtained from the Caenorhabditis Soil samples for the study were collected from three sites at farms Genetics Center (Table1) using six microsatellite loci amplified with in three provinces of South Africa; North West, Mpumalanga and primers listed in supplementary material TableS1. Kwa Zulu-Natal.
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