bioRxiv preprint doi: https://doi.org/10.1101/313494; this version posted May 3, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. An ancestral role of pericentrin in centriole formation through SAS-6 recruitment Daisuke Ito1*, Sihem Zitouni1, Swadhin Chandra Jana1, Paulo Duarte1, Jaroslaw Surkont1, Zita Carvalho-Santos1,2, José B. Pereira-Leal1, Miguel Godinho Ferreira1,3 and Mónica Bettencourt-Dias1* 1. Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, Oeiras 2780-156, Portugal. 2. Present address: Champalimaud Centre for the Unknown, Lisbon, Portugal 3. Institute for Research on Cancer and Aging of Nice (IRCAN), INSERM U1081 UMR7284 CNRS, 06107 Nice, France *Correspondence to:
[email protected] (DI);
[email protected] (MBD) Running title: Ancestral role of pericentrin-SAS-6 interaction Key words: centrosome, centriole, fission yeast, SPB, SAS-6, pericentrin, calmodulin, evolution Short summary The pericentriolar matrix (PCM) is not only important for microtubule-nucleation but also can regulate centriole biogenesis. Ito et al. reveal an ancestral interaction between the centriole protein SAS-6 and the PCM component pericentrin, which regulates centriole biogenesis and elongation. 1 bioRxiv preprint doi: https://doi.org/10.1101/313494; this version posted May 3, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract The centrosome is composed of two centrioles surrounded by a microtubule-nucleating pericentriolar matrix (PCM). Centrioles regulate matrix assembly. Here we ask whether the matrix also regulates centriole assembly.